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固定化脲素酶的活化载体制备的方法 被引量:1
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作者 汪瑾 朱连奎 《南京医学院学报》 CSCD 1989年第2期143-145,共3页
酶是参与机体多种化学反应的生物催化剂。在近代生物化学的发展中,酶的应用更加广泛,但酶对热、强酸、强碱、有机溶媒不稳定,很快失活。近年来通过载体活化制备固定化酶,它具有酶特有的催化活性,不溶于水和高的稳定性。因此在研究酶的利... 酶是参与机体多种化学反应的生物催化剂。在近代生物化学的发展中,酶的应用更加广泛,但酶对热、强酸、强碱、有机溶媒不稳定,很快失活。近年来通过载体活化制备固定化酶,它具有酶特有的催化活性,不溶于水和高的稳定性。因此在研究酶的利用,特别是制作生物化学传染器上是非常有利的。本实验是以光谱纯SiO_2颗粒及含SiO_260%的玻璃纤维膜作为固定脲素酶载体。采用氨基硅烷化和戊二醛交联的方法制备了固定脲素酶的活化载体。经固定化效果检验。 展开更多
关键词 固定化脲素酶 活化载体 制备
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硅胶负载型聚烯烃催化剂(Ⅰ)──不同类型硅胶载体热活化颗粒的TEM和SAED研究 被引量:5
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作者 刘柏平 任晓红 +1 位作者 阳永荣 戎顺熙 《中国有色金属学报》 EI CAS CSCD 北大核心 1998年第S2期26-29,共4页
利用TEM和选区电子衍射(SAED)方法对T-1,T-3和T-4三种硅胶载体原料及其受热活化过程的影响进行了研究,阐明了各种硅胶载体颗粒中原级粒子的尺度、形态和结合特征,以及它们受不同热活化条件的影响。并发现了600℃热活化使T-3和T-4... 利用TEM和选区电子衍射(SAED)方法对T-1,T-3和T-4三种硅胶载体原料及其受热活化过程的影响进行了研究,阐明了各种硅胶载体颗粒中原级粒子的尺度、形态和结合特征,以及它们受不同热活化条件的影响。并发现了600℃热活化使T-3和T-4硅胶载体颗粒发生局部烧结现象的实验依据。 展开更多
关键词 聚烯烃催化剂 硅胶载体活化 TEM SAED
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硅胶负载型聚烯烃催化剂(Ⅱ)──热活化硅胶载体及催化剂颗粒的TEM和SAED研究 被引量:4
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作者 刘柏平 任晓红 +1 位作者 阳永荣 戎顺熙 《中国有色金属学报》 EI CAS CSCD 北大核心 1998年第S2期30-32,共3页
利用TEM和选区电子衍射(SAED)的方法对T-1硅胶载体颗粒在800℃和900℃热活化条件下的变化进行了研究,阐明了硅胶载体颗粒中原级粒子的尺度、形态和结合特征等受热活化条件的影响。并发现了T-1硅胶载体颗粒发生局部烧结现象起始温度为... 利用TEM和选区电子衍射(SAED)的方法对T-1硅胶载体颗粒在800℃和900℃热活化条件下的变化进行了研究,阐明了硅胶载体颗粒中原级粒子的尺度、形态和结合特征等受热活化条件的影响。并发现了T-1硅胶载体颗粒发生局部烧结现象起始温度为900℃的实验依据。对催化剂颗粒的研究表明,在一定的催化剂组分配比条件下,组分的负载化对热活化载体颗粒的结构形态影响很小,催化剂的物相结构特征不会改变。 展开更多
关键词 聚烯烃催化剂 硅胶载体活化 TEM SAED
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乙二胺羟丙基壳聚糖固定化天门冬酰胺酶的研究 被引量:8
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作者 方波 王任远 +3 位作者 宋道云 周纪宁 江体乾 陈鸿雁 《华东理工大学学报(自然科学版)》 CAS CSCD 北大核心 2001年第4期341-343,348,共4页
研制了新型壳聚糖胺基衍生物——乙二胺羟丙基壳聚糖 ( EDA- HPCS) ,并将其用于固定天门冬酰胺酶。分别考察了甲醛活化载体和戊二醛活化载体对固定化酶活力的影响。结果表明 ,在适当的固定化条件下 ,甲醛活化 EDA- HPCS的固定化酶活力约... 研制了新型壳聚糖胺基衍生物——乙二胺羟丙基壳聚糖 ( EDA- HPCS) ,并将其用于固定天门冬酰胺酶。分别考察了甲醛活化载体和戊二醛活化载体对固定化酶活力的影响。结果表明 ,在适当的固定化条件下 ,甲醛活化 EDA- HPCS的固定化酶活力约为 30 U/g载体 ,而戊二醛活化EDA- HPCS固定化酶活力约为 1 展开更多
关键词 天门冬酰胺酶 固定化酶 壳聚糖胺基衍生物 乙二胺羟丙基壳聚糖 活化载体 蛋白质合成
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酶传感器中GOD酶固定化方法条件的选择 被引量:2
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作者 朱玲 安哲 《哈尔滨医科大学学报》 CAS 2002年第4期322-323,共2页
目的 研究尼龙网上胆碱氧化酶的不同固定化方法对酶活性的影响 ,以及测定体系pH值的选择。方法 采用活化载体 共价结合法和戊二醛交联法。结果 发现用改进后的活化载体 共价结合法固定的酶活性高 ,使用寿命长 ,酶不易损失 ,重现性好。
关键词 生物传感器 酶固定化 胆碱氧化酶 活化载体-共价结合法 戊二醛交联法
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要不断探索思想政治工作新途径
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作者 曹海军 吴斯 杨伟红 《黑龙江金融》 2000年第7期44-44,共1页
关键词 思想政治工作 新途径 思想政治工作网络化 管理机制 思想政治教育 金融业 新时期 多轮驱动 活化载体 安达市
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A palladium single-atom catalyst toward efficient activation of molecular oxygen for cinnamyl alcohol oxidation 被引量:3
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作者 Qinghao Shang Nanfang Tang +6 位作者 Haifeng Qi Shuai Chen Guoliang Xu Chuntian Wu Xiaoli Pan Xiaodong Wang Yu Cong 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 北大核心 2020年第12期1812-1817,共6页
Selective aerobic oxidation of alcohols under mild conditions is of great importance yet challenging,with the activation of molecular oxygen(O2)as a crucial capability of the catalysts.Herein,we demonstrate that an Al... Selective aerobic oxidation of alcohols under mild conditions is of great importance yet challenging,with the activation of molecular oxygen(O2)as a crucial capability of the catalysts.Herein,we demonstrate that an Al2O3-supported Pd single-atom catalyst leads to higher activity and selectivity compared to Pd nanoparticles for the oxidation of cinnamyl alcohol.The Al2O3 support used in this study is rich in coordinately unsaturated Al3+sites,which are apt for binding to Pd atoms through oxygen bridges and present a distinct metal-support interaction(MSI).The suitable MSI then leads to a unique electronic characteristic of the Pd single atoms,which can be confirmed via X-ray photoelectron spectroscopy,normalized X-ray absorption near-edge structure,and diffuse reflectance Fourier transform infrared spectroscopy.Moreover,this unique electronic state is proposed to be responsible for its high catalytic activity.With the help of in-situ UV-vis spectra and electron spin resonance spectra,a specific alcohol oxidation route with O2 activation mechanism is then identified.Active oxygen species behaving chemically like singlet-O2 are generated from the interaction of O2 with Pd1/Al2O3,and then oxidize the partially dehydrogenated intermediates produced by the adsorbed allylic alcohols and Pd atoms to the desired alkenyl aldehyde.This work provides a promising path for the design and development of high-activity catalysts for aerobic oxidation reactions. 展开更多
关键词 Single-atom catalysis PALLADIUM Alcohol oxidation Oxygen activation Metal support interaction
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Effects of the Supports on Activity of Supported Nickel Catalysts for Hydrogenation of m-Dinitrobenzene to m-Phenylenediamine 被引量:10
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作者 刘迎新 陈吉祥 张继炎 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2007年第1期63-67,共5页
The hydrogenation of m-dinitrobenzene to m-phenylenediamine in liquid phase was studied with the nickel catalysts supported on SiO2, TiO2, γ-Al2O3, MgO and diatomite carders. Based on the experiments of X-ray diffrac... The hydrogenation of m-dinitrobenzene to m-phenylenediamine in liquid phase was studied with the nickel catalysts supported on SiO2, TiO2, γ-Al2O3, MgO and diatomite carders. Based on the experiments of X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), temperature-programmed reduction (TPR), temperature-programmed desorption of hydrogen (H2-TPD) and activity evaluation, the physico-chemical and catalytic properties of the catalysts were investigated. Among the catalysts tested, the SiO2 supported nickel catalyst showed the highest activity and selectivity towards m-phenylenediamine, over which 97.3% m-dinitrobenzene conversion and 95.1% m-phenylenediamine yield were obtained at 373K under hydrogen pressure of 2.6MPa after reaction for 6 h when using ethanol as solvent. Although TiO2 and diatomite supported nickel catalysts also presented high activity, they had lower selectivity towards m-phenylenediamine. As for γ-Al2O3 and MgO supported catalysts were almost inactive for the object reaction. It was shown that both the activity and selectivity of the catalysts were strongly depended on the interaction between nickel and the support. The higher activities of Ni/SiO2, Ni/TiO2 and Ni/diatomite could be attributed to the weaker metal-support interaction, on which Ni species presented as crystallized Ni metal particles. On the other hand, there existed strong metal-support interaction in Ni/MgO and Ni γ-Al2O3, which causes these catalysts more difficult to be reduced and the availability of Ni active sites decreased, resulting in their low catalytic activity. 展开更多
关键词 HYDROGENATION M-PHENYLENEDIAMINE M-DINITROBENZENE supported nickel catalyst metal-support interaction
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Catalytic methanation of syngas over Ni-based catalysts with different supports 被引量:3
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作者 Yincong Liu Lingjun Zhu +5 位作者 Xiaoliu Wang Shi Yin Furong Leng Fan Zhang Haizhou Lin Shurong Wang 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2017年第5期602-608,共7页
Co-precipitation method was selected for the preparation of Ni/Al_2O_3, Ni/ZrO_2 and Ni/CeO_2 catalysts, and their performances in methanation were investigated in this study. The structure and surface properties of t... Co-precipitation method was selected for the preparation of Ni/Al_2O_3, Ni/ZrO_2 and Ni/CeO_2 catalysts, and their performances in methanation were investigated in this study. The structure and surface properties of these catalysts were characterized by BET, XRD, H_2-TPD, TEM and H_2-TPR. The results showed that the catalytic activity at low temperature followed the order: Ni/Al_2O_3>Ni/ZrO_2>Ni/CeO_2. Ni/Al_2O_3 catalyst presented the best catalytic performance with the highest CH_4 selectivity of 94.5%. The characterization results indicated that the dispersion of the active component Ni was the main factor affecting the catalytic activity and the one with higher dispersion gave better performance. 展开更多
关键词 Methanation Ni dispersion Catalytic activity Catalyst support Stability
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Top-down synthesis strategies: Maximum noble-metal atom efficiency in catalytic materials 被引量:1
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作者 Yaxin Chen Zhiwei Huang +3 位作者 Xiao Gu Zhen Ma Jianmin Chen Xingfu Tang 《Chinese Journal of Catalysis》 CSCD 北大核心 2017年第9期1588-1596,共9页
Top‐down synthesis has been used to prepare catalytic materials with nanometer sizes,but fabricating atomically dispersed metal catalysts remains a challenge because surface single metal atoms are prone to aggregatio... Top‐down synthesis has been used to prepare catalytic materials with nanometer sizes,but fabricating atomically dispersed metal catalysts remains a challenge because surface single metal atoms are prone to aggregation or coalescence.A top‐down strategy is used to synthesize atomically dispersed metal catalysts,based on supported Ag nanoparticles.The changes of the geometric and electronic structures of the Ag atoms during the top‐down process are studied using the in situ synchrotron X‐ray diffraction technique,ex situ X‐ray absorption spectroscopy,and transmission electron microscopy.The experimental results,coupled with the density functional theory calculations,demonstrate that the electronic perturbation of the Ag frontier orbitals,induced by the Ag‐O interactions at the perimeter of the metal‐support interface,is the driving force of the top‐down process.The top‐down synthesis has two important functions:to increase the number of catalytic active sites and to facilitate the study of complex reaction mechanisms(e.g.,formaldehyde oxidation)by developing single‐site model catalysts. 展开更多
关键词 Top‐down synthesis Atomic dispersion Catalytic active site Electronic metal‐support interaction Formaldehyde oxidation
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Promoting NO_(x)reduction via in situ activation of perovskite supported Pd catalysts under alternating lean-burn/fuel-rich operating atmospheres 被引量:1
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作者 Dongyue Zhao Yuexi Yang +6 位作者 Zhongnan Gao Mengxin Yin Ye Tian Jing Zhang Zheng Jiang Xiaobo Yu Xingang Li 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 2021年第5期795-807,共13页
Herein,we report the excellent De-NO_(x)performance of La0.7Sr0.3MnO3(LSM)perovskite-supported Pd catalysts(Pd-LSM)in alternating lean-burn/fuel-rich atmospheres using C3H6 as reductant and describe the in situ activa... Herein,we report the excellent De-NO_(x)performance of La0.7Sr0.3MnO3(LSM)perovskite-supported Pd catalysts(Pd-LSM)in alternating lean-burn/fuel-rich atmospheres using C3H6 as reductant and describe the in situ activation of the Pd catalysts via metal-support interaction(MSI)tuning.The NO_(x)reduction conversion of the Pd-LSM catalyst increased significantly from 56.1%to 90.1%and the production of N2O was suppressed.Our results demonstrated that this behavior was mainly attributed to the in situ transformation of Pd2+into Pd0 during the reaction.The generated Pd0 species could readily activate the C3H6 reductant and achieve an eight-fold higher turnover frequency than Pd2+for the reduction of NO_(x).Moreover,excessive MSIs inhibited the in situ generation of Pd0,and thereby,lowered the De-NO_(x)activity of the catalyst even at high Pd dispersion.In addition,the Pd-LSM catalysts exhibited much higher S tolerance than conventional Al_(2)O_(3)-supported catalysts.Our study provides a new approach for analyzing and designing highly active metal catalysts operated under dynamic alternating oxidizing/reducing atmospheric conditions. 展开更多
关键词 LEAN-BURN NO_(x)reduction Metal-support interactions In situ activation Pd
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Acetylene hydrochlorination over supported ionic liquid phase(SILP)gold-based catalyst:Stabilization of cationic Au species via chemical activation of hydrogen chloride and corresponding mechanisms 被引量:7
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作者 Jia Zhao Saisai Wang +9 位作者 Bolin Wang Yuxue Yue Chunxiao Jin Jinyue Lu Zheng Fang Xiangxue Pang Feng Feng Lingling Guo Zhiyan Pan Xiaonian Li 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 2021年第2期334-346,共13页
The activation of HCl by cationic Au in the presence of C2H2 is important for the construction of active Au sites and in acetylene hydrochlorination.Here,we report a strategy for activating HCl by the Au-based support... The activation of HCl by cationic Au in the presence of C2H2 is important for the construction of active Au sites and in acetylene hydrochlorination.Here,we report a strategy for activating HCl by the Au-based supported ionic liquid phase(Au–SILP)technology with the[N(CN)2^–]anion.This strategy enables HCl to accept electrons from[N(CN)2^–]anions in Au–[N(CN)2^–]complexes rather than from pure[Bmim][N(CN)2],leading to notable improvement in both the reaction path and the stability of the catalyst without changing the reaction triggered by acetylene adsorption.Furthermore,the induction period of the Au–SILP catalyst was shown to be absent in the reaction process due to the high Au(III)content in the Au(Ⅲ)/Au(Ⅰ)site and the high substrate diffusion rate in the ionic liquid layer.This work provides a facile method to improve the stability of Au-based catalysts for acetylene hydrochlorination. 展开更多
关键词 Acetylene hydrochlorination Electron density Hydrogen chloride activation Stabilization mechanism Gold-based supported ionic liquid phase catalyst
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Lentiviral vector-mediated down-regulation of IL-17A receptor in hepatic stellate cells results in decreased secretion of IL-6 被引量:3
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作者 Sheng-Chu Zhang Yi-Hu Zheng +5 位作者 Pan-Pan Yu Tan Hooi Min Fu-Xiang Yu Chao Ye Yuan-Kang Xie Qi-Yu Zhang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第28期3696-3704,共9页
AIM: To investigate the mechanism of interleukin (IL)-6 secretion through blocking the IL-17A/IL-17A recepto (IL-17RA) signaling pathway with a short hairpin RNA (shRNA) in hepatic stellate cells (HSCs) in vitro . MET... AIM: To investigate the mechanism of interleukin (IL)-6 secretion through blocking the IL-17A/IL-17A recepto (IL-17RA) signaling pathway with a short hairpin RNA (shRNA) in hepatic stellate cells (HSCs) in vitro . METHODS: HSCs were derived from the livers of adul male Sprague-Dawley rats. IL-6 expression was evalu ated using real-time quantitative polymerase chain reaction and enzyme linked immunosorbent assay. The phosphorylation activity of p38 mitogen activated pro tein kinases (MAPK) and extracellular regulated pro tein kinases (ERK) 1/2 upon induction by IL-17A and suppression by IL-17RA shRNA were examined using Western blotting.RESULTS: IL-6 expression induced by IL-17A was significantly increased compared to control in HSCs (P < 0.01 in a dose-dependent manner). Suppression of IL17RA using lentiviral-mediated shRNA inhibited IL-6 expression induced by IL-17A compared to group with only IL-17A treatment (1.44 ± 0.17 vs 4.07 ± 0.43, P < 0.01). IL-17A induced rapid phosphorylation of p38 MAPK and ERK1/2 after 5 min exposure, and showed the strongest levels of phosphorylation of p38 MAPK and ERK1/2 at 15 min in IL-17A-treated HSCs. IL-6 mRNA expression induced by IL-17A (100 ng/mL) for 3 h exposure was inhibited by preincubation with specific inhibitors of p38 MAPK (SB-203580) and ERK1/2 (PD-98059) compared to groups without inhibitors preincubation (1.67 ± 0.24, 2.01 ± 0.10 vs 4.08 ± 0.59, P < 0.01). Moreover, lentiviral-mediated IL-17RA shRNA 1 inhibited IL-17A-induced IL-6 mRNA expression compared to random shRNA in HSCs (1.44 ± 0.17 vs 3.98 ± 0.68, P < 0.01). Lentiviral-mediated IL17RA shRNA 1 inhibited phosphorylation of p38 MAPK and ERK1/2 induced by 15 min IL-17A (100 ng/mL) exposure. CONCLUSION: Down-regulation of the IL-17RA receptor by shRNA decreased IL-6 expression induced by IL-17A via p38 MAPK and ERK1/2 phosphorylation in HSCs. Suppression of IL-17RA expression may be a strategy to reduce the inflammatory response induced by IL-17A in the liver. 展开更多
关键词 Interleukin 17A Interleukin 6 Hepatic stellate cells Liver fibrosis
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结合化学知识探讨ATP水解放能实质 被引量:1
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作者 蒋选荣 姜维萍 《生物学通报》 CAS 2021年第8期12-14,共3页
结合化学学科知识,从基团转移、化学键的断裂和形成等方面,阐明了ATP作为活化载体分子的实质就是在磷酰基(-PO_(3)^(2-))和磷酸基团(-OPO_(3)^(2-))转化过程中,将多余的能量用于物质结合过程;从化学键的角度,阐明了ATP提供生命活动能量... 结合化学学科知识,从基团转移、化学键的断裂和形成等方面,阐明了ATP作为活化载体分子的实质就是在磷酰基(-PO_(3)^(2-))和磷酸基团(-OPO_(3)^(2-))转化过程中,将多余的能量用于物质结合过程;从化学键的角度,阐明了ATP提供生命活动能量的实质是在酶的催化下,通过化学键的断裂和生成过程中产生的自由能负差,促进物质合成反应的进行;从热力学角度,阐明ATP分子耦合细胞内化学反应的生理机制。 展开更多
关键词 ATP 活化载体分子 化学键耦合反应
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