[Objective] The aim was to optimize the fermentation medium and conditions of antibiotic active substances produced by Antarctic psychrotrophic bacterium Rheinheimera sp.97.[Method] Single-factor experiment and orthog...[Objective] The aim was to optimize the fermentation medium and conditions of antibiotic active substances produced by Antarctic psychrotrophic bacterium Rheinheimera sp.97.[Method] Single-factor experiment and orthogonal test were adopted to optimize the fermentation medium of antibiotic active substances produced by Antarctic psychrotrophic bacterium R.sp.97,while the fermentation conditions were optimized by single-factor experiment.[Result] The optimum fermentation medium for the antibiotic active substances production was as follows：tryptone 3.0 g/L,ammonium sulfate 1.0 g/L,starch 2.0 g/L,NaCl 15.0 g/L.The optimized fermentation conditions were as follows：the starting pH of medium was 8.0,fermentation temperature was 10 ℃,liquid volume in Erlenmeyer flask was 30 %（V/V）and inoculation amount was 1%（V/V）.Under the optimized fermentation medium and conditions,the antibacterial activity of R.sp.97 was increased by 18.1%.[Conclusion] This study had provided basis for the antibiotic active substances produced by Antarctic psychrotrophic bacterium R.sp.97.展开更多
[Objective] The aim was to study if the fermentation broth of Kombucha has protein with antimicrobial activity. [Method] The effects of different cultivation time on cell concentration, pH, total protein concentration...[Objective] The aim was to study if the fermentation broth of Kombucha has protein with antimicrobial activity. [Method] The effects of different cultivation time on cell concentration, pH, total protein concentration and inhibition zone were studied. The fermentation broth of Kombucha on the sixth day was adjusted to different pH. Fermentation broth treated with protease was put into the plates of Escherichia co/i, Bacillus cereue and Staphylococcus aureus as control, as well as ampenicilin. The zone of broth inhibition was measured. [ Result] The concentration of general protein was of positive relevance to the antimicrobial activity of Kombucha fermentation broth. The zone of inhibition decreased with rising pH and was the lowest when pH was 7. Later, as pH increasing, it enlarged. The inhibition effect of processed protease reduced remarkably. [ Conclusion] The fermentation broth of Kombucha revealed antimicrobial proteins.展开更多
The improvement of propionic acid production for antifungal activity, as fermenting by calcium alginate immobilized cells of Propionibacterium acidipropionici TISTR 442 was investigated by using whey as substrate. Opt...The improvement of propionic acid production for antifungal activity, as fermenting by calcium alginate immobilized cells of Propionibacterium acidipropionici TISTR 442 was investigated by using whey as substrate. Optimal condition for immobilization was performed by adjusting tube distance to CaCI2 solution to be 4-6 cm and 7 mL/min flow rate of alginate gel. The production of propionic acid by immobilized cells in a 2 L fermentor using 1% CaCO3 and 5 N KOH to control the pH at 6.5 gave maximum propionic acid and they had consistent potential to recycle 2 rounds of fermentation and produced the total of 29.24 g/L propionic acid （15.85 ± 0.25 g/L and 13.39 ± 0.25 g/L propionic acid from Batch 1 and Batch 2 fermentation, respectively）. Compared to free-cell fermentation, propionic acid productivity increased 20% （0.083 g/h vs. 0.070 g/h） and fermentation time reduced 11% （192 h vs. 216 h） in 2 L fermentor with 40 g/L initial total sugar from whey. The fermented propionic acid as well as the commercial propionic acid from chemical process was able to inhibit the growth of the fungal tested.展开更多
Coriolus versicolor has been reported to have biological activities such as anticancer, antibacterial, antibiotics and immunopotentiation. The authors tried to ferment using lactobacilli associated with various biolog...Coriolus versicolor has been reported to have biological activities such as anticancer, antibacterial, antibiotics and immunopotentiation. The authors tried to ferment using lactobacilli associated with various biological functions and expected that fermented extract by lactobacilli will be superior to non-fermented extract in antioxidant activity. C. versicolor was extracted with 70% ethanol and then it was fermented by Lactobacillus acidophilus KCTC3164 and Lactobacillus plantarum KCTC3099, respectively. 1,l-dipheny｜-2-picrylhydrazyl （DPPH） scavenging activity, superoxide dismutase （SOD）-like activity and tetrazolium salt 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assays were performed. DPPH scavenging activity of fermented extract by L. acidophilus was higher than fermented extract by L. plantarum and antioxidant effect of fermented extract was 20%-30% higher than non-fermented extract. SOD-like activity was represented 18% in fermented extract by L. acidophilus, and was 27% in fermented extract by L. plantarum higher than non-fermented extract. As a result, this study suggests that it may need an efficient protocol for dominant bio-physiological activities such as extract volume or appropriate working volume of cultured cell or mixture condition. The fermented C. versicolor extract should provide with useful antioxidant agent in the human skin.展开更多
基金Supported by National Department Public Benefit Marine Scientific Research Foundation(201005032-2)National High Technology Research and Development Program of China(2007AA091905)~~
文摘[Objective] The aim was to optimize the fermentation medium and conditions of antibiotic active substances produced by Antarctic psychrotrophic bacterium Rheinheimera sp.97.[Method] Single-factor experiment and orthogonal test were adopted to optimize the fermentation medium of antibiotic active substances produced by Antarctic psychrotrophic bacterium R.sp.97,while the fermentation conditions were optimized by single-factor experiment.[Result] The optimum fermentation medium for the antibiotic active substances production was as follows：tryptone 3.0 g/L,ammonium sulfate 1.0 g/L,starch 2.0 g/L,NaCl 15.0 g/L.The optimized fermentation conditions were as follows：the starting pH of medium was 8.0,fermentation temperature was 10 ℃,liquid volume in Erlenmeyer flask was 30 %（V/V）and inoculation amount was 1%（V/V）.Under the optimized fermentation medium and conditions,the antibacterial activity of R.sp.97 was increased by 18.1%.[Conclusion] This study had provided basis for the antibiotic active substances produced by Antarctic psychrotrophic bacterium R.sp.97.
基金Supported by Scientific Research Fund of Beijing Educational DepartmentBeijing Higher School Personnel Teaching Program
文摘[Objective] The aim was to study if the fermentation broth of Kombucha has protein with antimicrobial activity. [Method] The effects of different cultivation time on cell concentration, pH, total protein concentration and inhibition zone were studied. The fermentation broth of Kombucha on the sixth day was adjusted to different pH. Fermentation broth treated with protease was put into the plates of Escherichia co/i, Bacillus cereue and Staphylococcus aureus as control, as well as ampenicilin. The zone of broth inhibition was measured. [ Result] The concentration of general protein was of positive relevance to the antimicrobial activity of Kombucha fermentation broth. The zone of inhibition decreased with rising pH and was the lowest when pH was 7. Later, as pH increasing, it enlarged. The inhibition effect of processed protease reduced remarkably. [ Conclusion] The fermentation broth of Kombucha revealed antimicrobial proteins.
文摘The improvement of propionic acid production for antifungal activity, as fermenting by calcium alginate immobilized cells of Propionibacterium acidipropionici TISTR 442 was investigated by using whey as substrate. Optimal condition for immobilization was performed by adjusting tube distance to CaCI2 solution to be 4-6 cm and 7 mL/min flow rate of alginate gel. The production of propionic acid by immobilized cells in a 2 L fermentor using 1% CaCO3 and 5 N KOH to control the pH at 6.5 gave maximum propionic acid and they had consistent potential to recycle 2 rounds of fermentation and produced the total of 29.24 g/L propionic acid （15.85 ± 0.25 g/L and 13.39 ± 0.25 g/L propionic acid from Batch 1 and Batch 2 fermentation, respectively）. Compared to free-cell fermentation, propionic acid productivity increased 20% （0.083 g/h vs. 0.070 g/h） and fermentation time reduced 11% （192 h vs. 216 h） in 2 L fermentor with 40 g/L initial total sugar from whey. The fermented propionic acid as well as the commercial propionic acid from chemical process was able to inhibit the growth of the fungal tested.
文摘Coriolus versicolor has been reported to have biological activities such as anticancer, antibacterial, antibiotics and immunopotentiation. The authors tried to ferment using lactobacilli associated with various biological functions and expected that fermented extract by lactobacilli will be superior to non-fermented extract in antioxidant activity. C. versicolor was extracted with 70% ethanol and then it was fermented by Lactobacillus acidophilus KCTC3164 and Lactobacillus plantarum KCTC3099, respectively. 1,l-dipheny｜-2-picrylhydrazyl （DPPH） scavenging activity, superoxide dismutase （SOD）-like activity and tetrazolium salt 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assays were performed. DPPH scavenging activity of fermented extract by L. acidophilus was higher than fermented extract by L. plantarum and antioxidant effect of fermented extract was 20%-30% higher than non-fermented extract. SOD-like activity was represented 18% in fermented extract by L. acidophilus, and was 27% in fermented extract by L. plantarum higher than non-fermented extract. As a result, this study suggests that it may need an efficient protocol for dominant bio-physiological activities such as extract volume or appropriate working volume of cultured cell or mixture condition. The fermented C. versicolor extract should provide with useful antioxidant agent in the human skin.
