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EGCG对过氧化氢造成的螺旋神经节细胞损害的抑制作用 被引量:9
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作者 刘国辉 谢鼎华 +2 位作者 朱纲华 伍伟景 葛圣雷 《听力学及言语疾病杂志》 CAS CSCD 2004年第5期317-320,i001,共5页
目的 探讨表没食子儿茶素没食子酸盐 [(- ) -epigallocatechin - (3) -gallate ,EGCG]对过氧化氢(H2 O2 )所致培养耳蜗螺旋神经节细胞 (spiralganglioncells ,SGC)氧化损伤的保护作用。方法 SGC在体外培养 2 4小时后 ,分别加入不同浓... 目的 探讨表没食子儿茶素没食子酸盐 [(- ) -epigallocatechin - (3) -gallate ,EGCG]对过氧化氢(H2 O2 )所致培养耳蜗螺旋神经节细胞 (spiralganglioncells ,SGC)氧化损伤的保护作用。方法 SGC在体外培养 2 4小时后 ,分别加入不同浓度 (2 5、5 0、10 0、2 0 0及 5 0 0 μmol L)的H2 O2 和 或EGCG(10、5 0及 10 0 μg ml)后继续培养 2 4小时 ,采用四唑盐 (MTT)比色试验和Hoechst332 5 8核染色法分别检测细胞活力和凋亡率 ,并以经典抗氧化剂维生素C为对照。结果 当H2 O2 浓度≥ 5 0 μmol L时 ,细胞存活率及细胞凋亡率与对照组相比明显下降 (P <0 .0 5 ) ,并随H2 O2浓度升高而加重。EGCG处理后 ,能明显提高细胞存活率 ,降低细胞凋亡率 (P <0 .0 5 ) ,随着EGCG浓度提高 ,其保护作用加强 ,且较VitC更具优势。结论 绿茶提取物EGCG在H2 O2 展开更多
关键词 耳蜗 螺旋神经节细胞 活性氧/过氧化氢 表没食子儿茶素没食子酸盐 细胞活力 凋亡
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Effects of Drought Stress on Activity of POD and CAT in Wheat during Generation Period 被引量:5
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作者 陈军 高贵珍 +2 位作者 方雪梅 张兴桃 吕晓娇 《Agricultural Science & Technology》 CAS 2013年第11期1529-1531,共3页
[Objective] This study aimed to investigate the changes in activity of POD and CAT in wheat during generation period under drought stress. [Method] Four wheat cultivars, including Yunong 51, Zhoumai 22, Jimai 19 and H... [Objective] This study aimed to investigate the changes in activity of POD and CAT in wheat during generation period under drought stress. [Method] Four wheat cultivars, including Yunong 51, Zhoumai 22, Jimai 19 and Huaimai 22, were selected as experimental materials and cultured under drought stress simulated by different concentrations of PEG6000, to determine the activity of POD and CAT. [Result] POD and CAT activity in wheat during generation period increased gradual y under stress of 5% and 15% PEG6000 from 0 to 120 h; POD and CAT activity in wheat under stress of 15% PEG6000 increased more compared with that under stress of 5% PEG6000; POD and CAT activity in wheat under stress of 25% and 35% PEG6000 increased first and then decreased. Stress duration significantly af-fected the activity of POD and CAT in wheat during generation period. [Conclusion] This study provided theoretical references for breeding drought-tolerant wheat. 展开更多
关键词 WHEAT Germination period POD activity -CAT activity
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Catalytic Oxidative Desulfurization of Gasoline Using Vanadium(V)-substituted Polyoxometalate/H_2O_2/Ionic Liquid Emulsion System 被引量:2
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作者 Ge Jianhua Zhou Yuming +1 位作者 Yang Yong Xue Mengwei 《China Petroleum Processing & Petrochemical Technology》 SCIE CAS 2012年第1期25-31,共7页
Aiming at deep desulfurization of gasoline,three amphiphilic catalysts [C18H37N(CH3)3]3+x [PMo12-xVxO40](x=1,2,or 3) were prepared and characterized.The amphiphilic vanadium(V)-substituted polyoxometalates were dissol... Aiming at deep desulfurization of gasoline,three amphiphilic catalysts [C18H37N(CH3)3]3+x [PMo12-xVxO40](x=1,2,or 3) were prepared and characterized.The amphiphilic vanadium(V)-substituted polyoxometalates were dissolved in water-immiscible ionic liquid([Bmim]PF6),forming a H2O2-in-[Bmim]PF6 emulsion desulfurization system with 30 m% H2O2 serving as the oxidant.The catalytic oxidation of sulfur-containing model oil has been studied in detail under various reaction conditions using this system.The ionic liquid emulsion system showed high catalytic oxidative activity in the treatment of commodity gasoline.Furthermore,the mechanism of catalytic oxidative desulfurization was also elaborated. 展开更多
关键词 DESULFURIZATION vanadium (V)-substituted polyoxometalates ionic liquid emulsion catalytic oxidation
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Inhibitory effect of Cr(Ⅵ) on activities of soil enzymes
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作者 彭兵 黄顺红 +3 位作者 杨志辉 柴立元 许友泽 苏长青 《Journal of Central South University》 SCIE EI CAS 2009年第4期594-598,共5页
To evaluate the influence of various Cr(Ⅵ) concentrations (0.05, 0.25, 0.50, 1.00 and 2.00 g/kg) on the activity of soil enzymes, the activities of catalase, polyphenol oxidase, dehydrogenase, alkaline phosphatase in... To evaluate the influence of various Cr(Ⅵ) concentrations (0.05, 0.25, 0.50, 1.00 and 2.00 g/kg) on the activity of soil enzymes, the activities of catalase, polyphenol oxidase, dehydrogenase, alkaline phosphatase in soils were investigated in the incubation experiment with a period of 35 d. The results indicate that all the tested Cr(Ⅵ) concentrations significantly inhibit dehydrogenase activity by over 70% after 35 d. The activity of alkaline phosphatase is slightly inhibited during the whole experiment except for on the day 7. Cr(Ⅵ) has no obvious effect on the activity of catalase in soil. On the contrary, Cr(Ⅵ) stimulates the activity of polyphenol oxidase. The results suggest that dehydrogenase activity can be used as an indicator for assessing the severity of chromium pollution. 展开更多
关键词 Cr(Ⅵ) SOIL POLLUTION enzyme activity INHIBITION
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