Effect of ammonia at different concentrations on aerobic granular sludge and activated sludge was investigated in this study. Meanwhile, bacterial diversity variation and ammonia oxidizing bacterium （AOB） quantifica...Effect of ammonia at different concentrations on aerobic granular sludge and activated sludge was investigated in this study. Meanwhile, bacterial diversity variation and ammonia oxidizing bacterium （AOB） quantification within both kinds of sludge were monitored by terminal restriction fragment length polymorphism （T-RFLP） and real-time PCR （RT-PCR） technique, respectively. The results showed that the COD removal of both kinds of sludge changed slightly when the ammonia removal efficiency decreased gradually with the ammonia concentration increased from 100 mg L^-1 to 500 mg L^-1 Furthermore, activated sludge demonstrated higher ammonia removal ability than that of aerobic granular sludge （10%- 16%）. As revealed by T-RFLP, activated sludge was of higher ammonia removal ability and more abounding bacterial diversity than that of aerobic granular sludge, suggesting that the bacterial diversity was probably relevant to the ammonia removal. The RT-PCR results indicated that the AOB population size of activated sludge and aerobic granular sludge were 2.80× 10^4-3.44× 10^4cells （g dried sludge）^-1 and 7.83×10^4-1.18×10^5cells （g dried sludge）^-1, respectively. There is no obvious positive correlation between the ammonia removal ability and number of AOB in both kinds of sludge.展开更多
基金Acknowledge： The study are supported by the Natural Science Foundation of Jiangsu Province （No. BK2005402） and National Natural Science Foundation （No. 30640018）.
文摘Effect of ammonia at different concentrations on aerobic granular sludge and activated sludge was investigated in this study. Meanwhile, bacterial diversity variation and ammonia oxidizing bacterium （AOB） quantification within both kinds of sludge were monitored by terminal restriction fragment length polymorphism （T-RFLP） and real-time PCR （RT-PCR） technique, respectively. The results showed that the COD removal of both kinds of sludge changed slightly when the ammonia removal efficiency decreased gradually with the ammonia concentration increased from 100 mg L^-1 to 500 mg L^-1 Furthermore, activated sludge demonstrated higher ammonia removal ability than that of aerobic granular sludge （10%- 16%）. As revealed by T-RFLP, activated sludge was of higher ammonia removal ability and more abounding bacterial diversity than that of aerobic granular sludge, suggesting that the bacterial diversity was probably relevant to the ammonia removal. The RT-PCR results indicated that the AOB population size of activated sludge and aerobic granular sludge were 2.80× 10^4-3.44× 10^4cells （g dried sludge）^-1 and 7.83×10^4-1.18×10^5cells （g dried sludge）^-1, respectively. There is no obvious positive correlation between the ammonia removal ability and number of AOB in both kinds of sludge.
