期刊文献+
共找到15篇文章
< 1 >
每页显示 20 50 100
抑制白细胞介素-17活性短肽疫苗对ApoE-/-小鼠免疫功能的影响
1
作者 王顺 《实用临床医药杂志》 CAS 2015年第3期126-127,142,共3页
白细胞介素17(IL-17)是近年来发现的又一新的细胞因子[1],它主要由活化的T淋巴细胞产生,能诱导多种基质细胞产生多种前炎性细胞因子和造血活性因子,如白细胞介素-6(IL-6),白细胞介素-8(IL-8),粒细胞集落刺激因子(GCSF),肿瘤坏... 白细胞介素17(IL-17)是近年来发现的又一新的细胞因子[1],它主要由活化的T淋巴细胞产生,能诱导多种基质细胞产生多种前炎性细胞因子和造血活性因子,如白细胞介素-6(IL-6),白细胞介素-8(IL-8),粒细胞集落刺激因子(GCSF),肿瘤坏死因子?α(TNF?α)、白细胞介素-1(IL-1),前列腺素E2(PGE2)等[2-3]。 展开更多
关键词 抑制IL-17活性短肽疫苗 APO E-/-小鼠 安全剂量 免疫功能
下载PDF
一种包含抗菌和抗毒素双重活性的炭疽疫苗的研究
2
作者 唐盛高 钟江 《国外医学(微生物学分册)》 2004年第3期48-48,共1页
美国现有预防炭疽的疫苗只针对炭疽毒素,而炭疽感染是一个双重的过程,既有细菌复制,也有毒素产生。本文研制了一种针对以上两种过程的新的双重活性疫苗DAAV(DAAV-1和DAAV-2),并且通过一系列方法比较,证明了这一疫苗在增强特异性抗... 美国现有预防炭疽的疫苗只针对炭疽毒素,而炭疽感染是一个双重的过程,既有细菌复制,也有毒素产生。本文研制了一种针对以上两种过程的新的双重活性疫苗DAAV(DAAV-1和DAAV-2),并且通过一系列方法比较,证明了这一疫苗在增强特异性抗体产生和体液免疫方面均有显著作用。 展开更多
关键词 炭疽疫苗 炭疽感染 双重活性疫苗 DAAV 体液免疫
下载PDF
抑制IL-17活性短肽疫苗的抗动脉粥样硬化的治疗作用
3
作者 王顺 《医学信息(医学与计算机应用)》 2014年第35期118-118,119,共2页
目的:将具有抑制IL-17活性的短肽疫苗主动免疫ApoE-/-小鼠,研究IL-17疫苗对动脉粥样硬化的治疗作用。方法选取8 w龄健康雄性ApoE-/-小鼠,分两组,一组用生理盐水腹腔注射,一组用IL-17疫苗(0002)腹腔注射,每组12只,分别于0、2、4、6 w共免... 目的:将具有抑制IL-17活性的短肽疫苗主动免疫ApoE-/-小鼠,研究IL-17疫苗对动脉粥样硬化的治疗作用。方法选取8 w龄健康雄性ApoE-/-小鼠,分两组,一组用生理盐水腹腔注射,一组用IL-17疫苗(0002)腹腔注射,每组12只,分别于0、2、4、6 w共免疫4次,实验结束后,测体重,以自动分析仪检测血清中血脂含量,留取心脏以油红染色的方法检测主动脉瓣环及主动脉大体标本的斑块面积及其成分分析。结果结果表明IL-17疫苗(0002)在未影响小鼠体重及血脂情况下显著的减少斑块面积(P<0.01)。结论通过诱导产生具有靶向阻断作用的中和性抗体来产生作用的IL-17疫苗,为动脉粥样硬化的防治提供新思路。 展开更多
关键词 抑制IL-17活性短肽疫苗 APOE-/-小鼠 动脉粥样硬化 斑块面积
下载PDF
法国新型非活性炭疽疫苗研究取得进展
4
《口岸卫生控制》 2001年第6期29-29,共1页
法国巴斯德研究所日前宣告,该所研制的新型非活性炭疽疫苗已经过动物实验。目前他们正在寻找合作伙伴,以期尽快做人体实验。
关键词 法国 活性炭疽疫苗 实验 免疫接种
下载PDF
最新流行性感冒活性病毒疫苗简介
5
作者 程娟 《中国医药情报》 2004年第1期54-54,共1页
2003年6月17日,美国FDA通过Flu—Mist,这是第一种喷鼻式管理的流感疫苗,即将在美国上市。也是美国通过的第一种活性病毒流感疫苗。FluMist用来预防在5~49岁的健康人体内由于感染A型和B型病毒引起的流感疾病。5~8岁的儿童在第一年使... 2003年6月17日,美国FDA通过Flu—Mist,这是第一种喷鼻式管理的流感疫苗,即将在美国上市。也是美国通过的第一种活性病毒流感疫苗。FluMist用来预防在5~49岁的健康人体内由于感染A型和B型病毒引起的流感疾病。5~8岁的儿童在第一年使用Flu—Mist流感疫苗时,两剂之间至少要相隔6周。9~49岁的人群只需一剂疫苗。 展开更多
关键词 流行性感冒 活性病毒疫苗 B型病毒 预防
下载PDF
肠道细菌性病原疫苗
6
作者 江丽君 陈锦荣 《生物制品快讯》 2003年第6期1-3,共3页
关键词 肠道细菌性病原体 肠道感染 活性口服疫苗 活性疫苗
下载PDF
妊娠期使用流感疫苗的安全性
7
作者 赵璐 《国外医学(妇产科学分册)》 2007年第3期221-221,共1页
妊娠妇女在流行性感冒的季节易患重症流感及肺部并发症。尽管实际上使用非活性流感疫苗对母婴均是安全的,但在这些高危人群中流感疫苗的使用率仍很低,其主要原因是对妊娠期使用流感疫苗的安全性认识不足。为评估流感疫苗在妊娠中晚期... 妊娠妇女在流行性感冒的季节易患重症流感及肺部并发症。尽管实际上使用非活性流感疫苗对母婴均是安全的,但在这些高危人群中流感疫苗的使用率仍很低,其主要原因是对妊娠期使用流感疫苗的安全性认识不足。为评估流感疫苗在妊娠中晚期使用的安全性,自位于德克萨斯州休斯顿的一家大型综合性医院1998年7月1日-2003年6月30日5个流感季节的电脑数据,选取7183对在6个月内接种非活性疫苗并且单胎的健康母婴样本,作为基本数据研究。用t检验以及x^2检验或F检验,对各组中的变量进行比较。 展开更多
关键词 流感疫苗 安全性 妊娠期 大型综合性医院 流感季节 活性疫苗 肺部并发症 流行性感冒
下载PDF
重组猪圆环病毒2型Cap蛋白在毕赤酵母中的优化表达及疫苗活性研究 被引量:2
8
作者 王斌 罗时渝 +2 位作者 谢景毅 叶燕锐 潘力 《现代食品科技》 EI CAS 北大核心 2018年第4期51-55,共5页
猪圆环病毒2型(PCV2)是引起断奶猪多系统衰竭综合症(PMWS)的主要致病原,在全世界范围内对养猪业造成了巨大的经济损失。猪圆环病毒2型Cap蛋白(PCV2-Cap)是制备猪蓝耳病疫苗的主要靶蛋白。针对目前PCV2疫苗生产工艺复杂、成本较高的问题... 猪圆环病毒2型(PCV2)是引起断奶猪多系统衰竭综合症(PMWS)的主要致病原,在全世界范围内对养猪业造成了巨大的经济损失。猪圆环病毒2型Cap蛋白(PCV2-Cap)是制备猪蓝耳病疫苗的主要靶蛋白。针对目前PCV2疫苗生产工艺复杂、成本较高的问题,本研究利用毕赤酵母表达系统研究PCV2-Cap蛋白的重组表达及疫苗活性,将PCV2-Cap基因序列去除核定位信号序列(NLS)后,经密码子偏好性优化,构建重组表达载体,并转化至毕赤酵母宿主菌X-33,经Zeocin抗性筛选获得重组菌株。SDS-PAGE和Western blot结果显示Cap蛋白在酵母表达系统中成功表达,蛋白质大小为26 ku。5 L罐高密度发酵小试,得到PCV2-Cap蛋白在发酵上清液中的浓度约为0.53 mg/m L,占总蛋白的23.5%。动物免疫试验显示重组Cap蛋白能刺激机体产生特异性抗体。结果表明,PCV2-Cap基因在毕赤酵母中实现了分泌表达,为PCV2-Cap毕赤酵母亚单位疫苗的研制提供了数据支持。 展开更多
关键词 猪圆环病毒2型 毕赤酵母 重组表达 疫苗活性
原文传递
猪支原体肺炎的防治措施 被引量:3
9
作者 徐淑艳 《吉林畜牧兽医》 2021年第10期37-38,共2页
随着养猪业逐渐实现规模化、集约化的饲养场生产模式,生产数量扩增,在饲养过程中传染性疾病发病率增高的问题也逐渐显现。猪支原体肺炎虽然被发现已久,但是在规模化饲养后,患病率明显增高,成为发病率最高、影响范围最广的疫病之一,造成... 随着养猪业逐渐实现规模化、集约化的饲养场生产模式,生产数量扩增,在饲养过程中传染性疾病发病率增高的问题也逐渐显现。猪支原体肺炎虽然被发现已久,但是在规模化饲养后,患病率明显增高,成为发病率最高、影响范围最广的疫病之一,造成了巨大经济损失的同时,也严重的影响了养猪业的良性发展。现阶段对猪支原体肺炎的预防主要以疫苗接种为主,我国主要以研发的弱毒疫苗为主,虽然免疫效果极佳,但是因为疫苗接种的方法与难度较大、养殖人员的专业性不足等问题一直得不到理想的预防效果。在患病猪只的治疗过程中,因为抗生素仅能起到抑制作用,能够避免并发症与二次感染,但是无法完全清楚病原,造成停药后反复复发,并且同一种药物长时间进行治疗还会使支原体对其产生耐药性,严重的影响了正常治疗与生产。本文将对猪支原体肺炎的防治措施与研究发展进行详细的阐述,为养殖户与相关工作人员提供相应的参考。 展开更多
关键词 猪支原体肺炎 弱毒性活疫苗 活性疫苗 抗生素
下载PDF
Preparation of Purified and Inactivated HP-PRRS Vaccine
10
作者 唐艳林 宋睿 +2 位作者 李静姬 苗丽娟 何玉友 《Agricultural Science & Technology》 CAS 2014年第6期937-941,共5页
[Objective] The aim of this study was to improve the purification and protective potency of HP-PRRS inactivated vaccine. [Method] HP-PRRS virus that had been multiplied inside Marc-145 cells was collected and concentr... [Objective] The aim of this study was to improve the purification and protective potency of HP-PRRS inactivated vaccine. [Method] HP-PRRS virus that had been multiplied inside Marc-145 cells was collected and concentrated 50 times and then inactivated. Complete virions were separated and collected by chromatography with Sepharose 4 Fast Flow. Oil adjuvant was added to prepare purified inactivated vaccine. [Result] Viral protein was separated from other proteins by purification and the viral protein contents ranged from 76.7% to 82.4%, and 96% of the expected serum proteins were removed. Protective potency of purified vaccine was above 4/5 and positive conversion rate of antibody was over 86%, both higher than that of unpurified vaccine. The differences were significant. [Conclusion] The experiment il-lustrated that the immune efficacy of vaccine can be enhanced through concentrat- ing and purifying, while the non-viral protein can be removed, so that allergic reaction and stress response cadsed by vaccine inoculation can be avoided. 展开更多
关键词 HP-PRRS purified inactivated vaccine Protective potency Positive con-version rate of antibody Stress response
下载PDF
Model for the Vaccination against the Poliomyelitis 被引量:1
11
作者 Irene Duarte Gandica Lina Marcela Ocampo +1 位作者 Edwin Femando Duque Matin Maria Mercedes Gonzáilez 《Journal of Mathematics and System Science》 2013年第2期62-67,共6页
The poliomyelitis is an acute infection produced by the polio virus that affects the human central nervous system. It is transmitted by fecal-oral and respiratory contact.There are two types of vaccine, OPV (live att... The poliomyelitis is an acute infection produced by the polio virus that affects the human central nervous system. It is transmitted by fecal-oral and respiratory contact.There are two types of vaccine, OPV (live attenuated virus) and IPV (inactivated polio virus). Currently, there is a plan of vaccination until the age of 5 with OPV. The children vaccinated expel a virus (derived from the vaccine) to the environment, and some of the people that have oral contact with them, get vaccinated by the herd behavior. Nevertheless, taking into account the lately observed facts about the reversion to virulence of the oral polio vaccine during its circulation in the environment, a change in the current vaccination schedule is being contemplated, where the oral polio vaccine can be replaced by the inactivated vaccine. Nowadays, In Colombia the inactivated oral polio vaccine is recommended for children presenting immune deficiency who are vaccinated with IPV. These children do not expel poliovirus to the environment. This work presents a mathematical model that describes the dynamics of the infection in a population where the two types of vaccination are carried out. The population is divided into two groups of age and Michaelis-Menten interactions. Different strategies of vaccination are simulated and analyzed. 展开更多
关键词 POLIOMYELITIS vaccine OPV vaccine IPV VACCINATION mathematical model.
下载PDF
Review for Porcine Reproductive and Respiratory Syndrome Virus
12
作者 Tao XING Xingxing YU +6 位作者 Jun ZHANG Hongfei ZHANG Haigen WANG Guangsheng ZHOU Min ZHANG Chuanmin LIU Jingui LI 《Agricultural Science & Technology》 CAS 2017年第1期147-150,179,共5页
Porcine reproductive and respiratory syndrome (PRRS) is the severest disease of pigs worldwide, caused by a highly genetically diverse RNA virus, called Porcine reproductive and respiratory syndrome virus (PRRSV).... Porcine reproductive and respiratory syndrome (PRRS) is the severest disease of pigs worldwide, caused by a highly genetically diverse RNA virus, called Porcine reproductive and respiratory syndrome virus (PRRSV). The research summarized the genome characteristics of PRRSV particles and the most updated knowledge of structure protein function, and introduced the intellectual of PRRSV transmission and host immune response, which is very important for prevention and control for PRRS. A report showed that mass vaccination can stabilize the immunity of the entire herd, and this is the first required step for a PRRS eradication plan. However, the attenuated live vaccines may not achieve a valid prevention. The final goal of the EU project is to develop new generation, efficacious and safe maker vaccines that can be adapted to temporary changes and geographical differences.Robinson reported that broadly antibodies could neutralize all rapidly evolving type Ⅰ and type Ⅱ viruses, while further studies are expected to elucidate mechanisms of neutralizing antibody production and maturation and to investigate conserved epitope targets of cross-neutralization in this rapidly evolving virus. 展开更多
关键词 Porcine reproductive and respiratory syndrome VIRUS VACCINE Broadly antibodies Prevention and control
下载PDF
Titration Method Validation of Live Vaccines against Infectious Bursal Disease
13
作者 Fatima Tahiri Khadija Id Sidi Yahia +1 位作者 Benaissa Attrassi Driss Belghyti 《Journal of Life Sciences》 2013年第10期1084-1102,共19页
Validating a method of analysis goes through different steps, which aims at testing the normality of measurements distribution, estimating the uncertainty of the components of a measurement (i.e., accuracy and correc... Validating a method of analysis goes through different steps, which aims at testing the normality of measurements distribution, estimating the uncertainty of the components of a measurement (i.e., accuracy and correctness), and finally, define the control tests of non degradation of the method performances. This paper outlines the steps for validating a biological method of analysis. It involves the construction of an experimental design, a statistical model, and the preparation of an interne laboratory reference material (pilot vaccine). The latter is used to study the impact of deviation and variation factors, in order to, optimize the analytical method, to evaluate the bias (random error), and to calculate the uncertainty of measurement, and make the control charts. This method is applied in the titration of live viral vaccines of Gumboro disease on chicken's embryos fibroblasts. The experimental results show that potential influence factors related to the titration method had no significant influence on the obtained results. Taking into account these results, an operating mode has been elaborated. The finalized method proved to be faithful to standard deviation of repeatability and reproducibility of 0.21 and 0.22, respectively, with a confidence level of 95%. The calculated uncertainty of measurement is equal to 0.2, which represents the average error level of a titer. A homogeneous stock of interne laboratory reference vaccine (MRIL), with an average titer of 5.9 log DIT 50, was produced and the control chart set in away to provide the laboratory with an important tool of control and monitoring of the viral titers evolution in time, as well as, the mastery of the validated titration method performances. 展开更多
关键词 Live vaccine of infectious bursal disease titration method characterization experimental design method validation ACCURACY UNCERTAINTY STATISTICS control chart.
下载PDF
Immunogenicity and safety of a new inactivated hepatitis A vac cine in young adults: a comparative study 被引量:3
14
作者 任爱国 冯福民 +2 位作者 马俊荣 徐应军 刘崇柏 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第10期1483-1485,共3页
OBJECTIVE: To evaluate the immunogenicity, safety, and dosage of a new inactivated hepatitis A vaccine administered to young adults. METHODS: One hundred and four normal adult volunteers, seronegative for hepatitis A ... OBJECTIVE: To evaluate the immunogenicity, safety, and dosage of a new inactivated hepatitis A vaccine administered to young adults. METHODS: One hundred and four normal adult volunteers, seronegative for hepatitis A virus and hepatitis B surface antigen, were randomly assigned to one of three groups. The high-dose group received a primary dose of 1000 units of the new vaccine, the low-dose group received a primary dose of 500 units of the same vaccine, and the Havrix group received a primary dose of 1440 enzyme-linked immunosorbent assay units of Havrix, a licensed inactivated hepatitis A vaccine. All groups received a booster dose of the same vaccine 6 months after the primary dose. Local and systemic adverse reactions, seroconversion rates, and geometric mean titers of hepatitis A virus antibodies were measured in all three groups. RESULTS: Local and systemic reaction types and rates were similar in all three groups after primary and booster doses, although local reactions were more frequent in the Havrix group following the primary dose. No serious adverse reactions occurred. One month after the primary dose, the seroconversion rate was 87.5% in the high-dose group, 70.0% in the low-dose group, and 50.0% in the Havrix group (P = 0.001, versus the high-dose group). At month 6 (before administration of the booster dose), seroconversion rates were 96.9% in the high-dose group, 65.0% in the low-dose group (P = 0.0029), and 68.8% in the Havrix group (P = 0.007). All subjects in all groups seroconverted by one month after receipt of the booster dose. Geometric mean titers were similar in all three groups at month 1, but were higher in the high-dose group (264 mIU/ml) than those in the Havrix group (135 mIU/ml) at month 6 (P = 0.0013). One month after the booster dose, geometric mean titers in the high-dose group (2747 mIU/ml) were higher than those in the low-dose group (1657 mIU/ml) (P = 0.0223) or in the Havrix group (1316 mIU/ml) (P = 0.01). CONCLUSIONS: This new inactivated hepatitis A vaccine is immunogenic and safe; two doses of either 500 or 1000 units can induce hepatitis A virus antibodies well above the protection level. 展开更多
关键词 Adolescent Adult Comparative Study Hepatitis A Vaccines Humans Research Support Non-U.S. Gov't Vaccines Attenuated
原文传递
炭疽毒素显性失活抑制剂的安全性和免疫原性
15
作者 蔡任之 《国际生物制品学杂志》 CAS 2006年第2期91-92,共2页
关键词 炭疽毒素 免疫原性 失活 显性 炭疽杆菌毒素 安全性 抑制剂 哈佛大学医学院 现行疫苗 双重活性疫苗
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部