玉米大斑病菌毒素的液相色谱分离及7种纯组分的活性监测

由玉米大斑病菌(HT-毒素)引起的玉米大斑病是世界玉米产区的重要病害之一,罹病的玉米植株叶片迅速萎蔫死亡,严重地威胁着玉米的产量,造成很大的经济损失.近年来由于大面积种植的玉米品种不抗病,大斑病日趋严重[1].

加氢精制反应器模拟与催化剂活性的软件监测

利用反应动力学模型及非等温动力学数据的分析方法，采用生产实际数据对加氢精制反应器进行计算机模拟，模型充分考虑了物流性质与操作条件的影响，数值模拟结果与实际情况吻合较好，得出转化率随床层的分布曲线等较为全面的信息。

利用稻瘟霉分生孢子跟踪分离面包海星中的海星皂苷活性成分

目的研究面包海星中的海星皂苷活性成分。方法以稻瘟霉模型进行活性监测,采用多种层析手段和波谱技术对海星皂苷活性成分跟踪分离并鉴定其结构。结果分离获得14个海星皂苷,鉴定了其中12个结构(1～12),包括7个新的海星皂苷(6～12)。其中大多对稻瘟霉显示活性且对K562和BEL7402肿瘤细胞株显示显著或中等强度的细胞毒性,2个皂苷有溶血作用。结论利用稻瘟霉模型对海星皂苷活性成分进行跟踪分离是可行的,该研究也为进一步开发抗肿瘤新药提供了有价值的先导化合物和科学依据。

Design of pharmacodynamic monitoring system based on acupuncture differential thermometry

To control drug effects by detecting temperature difference between biologically active point（BAP）and intact area of skin for treatment of mental illness,a device is developed for monitoring the temperature of BAP and the dose medication and its change in real time to increase effectiveness of treatment.Two electrodes by Foll R method are used and BAP is determined based on topographic anatomical reference points.The temperature values are measured by integral thermometers DS18B20.the received data are processed and temperature difference is calculated and displayed under the control of microcontroller Atmega32.The obtained data confirm the correlation between the temperature difference indicators BAP C7,Gi4 and neurological scales assessing severity of mental illness.The experimetal results show that the temperature difference can be criteria for evaluating the effects of drugs,which is the basis for computer control systems of of medical process of mental patients.

A New Indicator Cell Line Established to Monitor Bovine Foamy Virus Infection

In order to improve the accuracy for quantitating the bovine foamy virus (BFV) in vitro,we developed a baby hamster kidney cell (BHK)-21-derived indicator cell line containing a plasmid that encodes the firefly luciferase driven by the BFV long terminal repeat promoter (LTR,from-7 to 1012).The BFV titer could be determined by detecting the luciferase expression since the viral trans-activator BTas protein activates the promoter activity of the LTR.One clone,designated BFVL,was selected from ten neomycin-resistant clones.BFVL showed a specific and inducible dose-and time-dependent luciferase activity in response to BFV infection.Although the changes in luciferase activity of BFVL peaked at 84 h post infection,it was possible to differentiate infected and uninfected cells at 48 h post infection.A linear relationship was established between the multiplicity of infection (MOI) of BFV and the activated ratio of luciferase expression in BFVL.Moreover,the sensitivity of the BFVL-based assay for detecting infectious BFV was 10,000 times higher than the conventional CPE-based assay at 48 h post infection.These findings suggest that the BFVL-based assay is rapid,easy,sensitive,quantitative and specific for detection of BFV infection.

Manila clam Venerupis philippinarum as a biomonitor to metal pollution

The Manila clam Venerupis philippinarum is a good biomonitor/bioindicator to marine metal pollution and is frequently used in aquatic toxicology. Two dominant pedigrees (white and zebra) of clam are distributed in the Bohai Sea; however, little attention has been paid to potential biological differences between these two pedigrees. In this study, we tested the sensitivity of both pedigrees to marine metal (cadmium and zinc) pollution biomonitoring and marine environmental toxicology. Results demonstrate significant biological differences in gills of white and zebra clams based on metabolic profiles and antioxidant enzyme activities. In addition, we found that hypotaurine, malonate and homarine were relatively high in white clam gills, while alanine, arginine, glutamate, succinate, 4-aminobutyrate, taurine and betaine were high in zebra clam gills. Zebra clam gills were also more sensitive to a mixture of Cd and Zn, as shown by antioxidant enzyme activities and metabolic profiles, but white clam gills could accumulate more Zn. Therefore, we suggest that the white pedigree can be used as a biomonitor to marine Zn pollution, whereas the zebra pedigree can be used for toxicology studies on Cd and Zn mixed pollution.

基于动物热活性检测系统分析动物热活性影响因素

目的基于动物热活性监测系统进行动物热活性影响因素分析,以期对中药寒热药性评价进行方法学探索。方法采用动物热活性监测系统,考察品种、性别及适应时间对ICR、BALB/c及KM种小鼠在不同温区停留时间比例（RTR）的影响。结果 ICR、BALB/c种小鼠可耐受最低温度为15℃,最高为40℃;与适应前比较,15℃温区内ICR、BALB/c、KM种小鼠分别在6、8、4h,30℃温区内分别在6、6、4h,40℃温区内分别在4、6、8h,RTR值均开始减少（P〈0.05）;雌、雄性KM小鼠在15~40℃温区的RTR值差异无统计学意义（P〉0.05）。结论在动物热活性监测系统中,3个品种小鼠的可耐受温度范围大致为15~40℃,累计适应4~8h后对温区间温差能形成相对稳定的记忆,且雌、雄性KM小鼠对同一温度的感知基本一致。

Cylex ImmuKnow测定稳定肝移植患者CD4＋T淋巴细胞活性临床分析

目的探讨中国人肝移植术后肝功能稳定患者ImmuKnow免疫细胞功能测定与临床免疫抑制状况的相关性。方法收集46例肝移植术后肝功能稳定患者的临床资料，选取性别、年龄、病因、血常规、肝功能、肾功能、乙肝五项和丙肝病毒载量、CD淋巴细胞亚群、他克莫司血药谷浓度、ImmuKnow值作为观察指标，对术后肝功能稳定患者ImmuKnow值与其他各相关临床指标进行多因素回归分析。结果肝移植术后（22±15）个月肝功能稳定患者ImmuKnow值为（203±114）ATP ng／ml（38．47～524．06ATP ng／ml）。回归分析显示ImmuKnow值与白细胞计数、CD3＋淋巴细胞绝对计数以及CD3＋CD4＋T淋巴细胞绝对计数有相关性（P〈0．05）。ImmuKnow值与他克莫司谷值浓度、他克莫司日用量以及患者性别、年龄及其他肝功能指标没有相关性（P〉0．05）。5例合并活动性丙型肝炎病毒感染者有较低的ImmuKnow值（〈61ATP ng／ml）。结论Cylex ImmuKnow检测可以用于稳定肝移植患者免疫抑制的监测，具有潜在的临床应用价值。综合评价肝移植患者免疫状态需要联合ImmuKnow、T淋巴细胞亚群、血药浓度、肝功能指标检测等相关检查，才能有效指导肝移植受体免疫抑制剂的个体化使用。

Effect of Temperature Variation on the Separation of Sesamin and Sesamolin by Simulated Moving Bed

In this work, sesamin and sesamolin is separated and purified by SMB （simulated moving bed） chromatography. Purity of sesamin and sesamolin can reach 99.2% and 99.9 %, and they can be recovered by 99.9% and 99.4%, respectively. Concentrations of the extract and raffinate was monitored and periodical change of the concentration was observed on experiment. Effects of non-linear adsorption, dead volume, and temperature on the separation are reviewed by the triangle theory to explain the periodical change of concentration. It is found that dead volume has little effect, and temperature fluctuation significantly affects thc separation by SMB. The temperature seriously affects the robust operation of SMB due to the change of Henry＇s constant, which will change the selectivity and restrain the productivity. Although the productivity in this study is only 0.0416 g/L-hr, the cost of the stationary phase used in this study is much lower than that of packed in analytical column. By using this stationary phase, the authors are still able to separate the mixture of sesamin and sesmolin. This will economically attract attention for the application of SMB to purify bioactive compounds on developing botanical drugs.

重组人血管内皮生长因子抑制剂中异天门冬氨酸含量检测

目的：建立对重组人血管内皮生长因子抑制剂进行异天门冬氨酸（Isoasp）含量检测的方法。方法：利用ISOQUANT异天门冬氨酸检测试剂盒并结合HPLC的方法检测重组人血管内皮生长因子抑制剂原液和成品中的Isoasp含量,并对该方法进行加标回收率试验、精密度和准确性的验证。结果：40、25、6.25 pmol·L-1的3种浓度对照品的加标回收率均在80%~120%的范围内。对1批原液和1批成品的各3个样品分别进行测定,原液和成品中每摩尔蛋白的Isoasp含量平均值分别为（0.066±0.010）、（0.093±0.008）mol,RSD分别为15.2%和8.6%,原液和成品中各1个样品的25 pmol·L-1对照品的加标回收率分别为90.2%和108.1%。结论：经方法学验证,本文所建立的方法可作为该产品的Isoasp含量的常规检测方法,也为融合蛋白及单抗类制品的Isoasp含量检测提供借鉴。

Response enhancement of olfactory sensory neurons-based biosensors for odorant detection

This paper presents a novel strategy for the response enhancement of olfactory sensory neurons (OSNs)-based biosensors by monitoring the enhancive responses of OSNs to odorants. An OSNs-based biosensor was developed on the basis of the light addressable potentiometric sensor (LAPS), in which rat OSNs were cultured on the surface of LAPS chip and served as sensing elements. LY294002, the specific inhibitor of phosphatidylinositol 3-kinase (PI3K), was used to enhance the responses of OSNs to odorants. The responses of OSNs to odorants with and without the treatment of LY294002 were recorded by LAPS. The results show that the enhancive effect of LY294002 was recorded efficiently by LAPS and the responses of this OSNs-LAPS hybrid biosensor were enhanced by LY294002 by about 1.5-fold. We conclude that this method can enhance the responses of OSNs-LAPS hybrid biosensors, which may provide a novel strategy for the bioelectrical signal monitor of OSNs in biosensors. It is also suggested that this strategy may be applicable to other kinds of OSNs-based biosensors for cellular activity detection, such as microelectrode array (MEA) and field effect transistor (FET).

血液透析滤过治疗维持性血液透析患者顽固性高血压的临床观察

探讨维持性血压透析患者顽固性高血压治疗中实施血液透析滤过的效果。方法:46例顽固性高血压患者用电脑系统随机排序法分为对照组23例、观察组23例,分别行维持性血液透析、血液透析滤过治疗。比较两组血压、血浆监测肾素活性(RA)及血管紧张素II(AngII)水平。结果:组间治疗后收缩压、舒张压、平均动脉压比,观察组均较低(P<0.05)。治疗后,两组血浆RA和AngII比,观察组均较低(P<0.05)。结论:维持性血液透析患者顽固性高血压治疗中实施血液透析滤过疗法,可控制血压以及平均动脉压水平,改善相关指标,具有可推广价值。