采用活性荧光黄FL和反应型抗紫外线整理剂UV-SUNCELLIQ对蚕丝织物进行染色和抗紫外线同浴整理，探讨了染色温度和时间，纯碱、氯化钠、紫外吸收剂和染料用量对蚕丝织物表观色深K／S值和紫外线防护指数UPF的影响，分析了染色织物的荧光...采用活性荧光黄FL和反应型抗紫外线整理剂UV-SUNCELLIQ对蚕丝织物进行染色和抗紫外线同浴整理，探讨了染色温度和时间，纯碱、氯化钠、紫外吸收剂和染料用量对蚕丝织物表观色深K／S值和紫外线防护指数UPF的影响，分析了染色织物的荧光效果、染色牢度和耐水洗性能。结果表明，染色蚕丝织物的K／S值和UPt’指数，随染色时间的延长和氯化钠用量的增加而逐渐升高；随着温度的升高和纯碱用量的增加，其K／S值和UPF指数先升高后降低；染料质量分数为2％～4％（om0时，染色蚕丝织物可满足警示服的荧光要求。当染料质量分数3%（omf），抗紫外线整理剂UV-SUN CEL LIQ5%（omf），纯碱0．5g／L，氯化钠50g／L，在70℃保温60min时，染色织物UPF指数接近120，且耐洗性很好，经30次标准洗涤后，其UPF指数仍超过90。展开更多
DNA-binding fluorochromes are often used for vital staining of plant cell nuclei. However, it is not always sure whether the cells after staining still remain in living state. We chose several criteria to estimate the...DNA-binding fluorochromes are often used for vital staining of plant cell nuclei. However, it is not always sure whether the cells after staining still remain in living state. We chose several criteria to estimate the validity of real vital staining for sexual cell nuclei. These were: the cytoplasmic streaming in pollen tubes whose nuclei were stained, the simultaneous visualization of fluo-rochromatic reaction and nucleus staining in isolated generative cells, and the capability of isolated, prestained generative or sperm cells to fuse with other protoplasts. The results confirmed that 4',6-diamidino-2-phenylindole (DAPI), Hoechst 33258 and mithramycin could be used as real vital stains, though their efficiency varied from case to case; among them DAPI showed best effect. The fluorescent vital staining technique offered a useful means fori-dentification and selection of heterokaryons in gametoplast manipulation studies.展开更多
文摘采用活性荧光黄FL和反应型抗紫外线整理剂UV-SUNCELLIQ对蚕丝织物进行染色和抗紫外线同浴整理，探讨了染色温度和时间，纯碱、氯化钠、紫外吸收剂和染料用量对蚕丝织物表观色深K／S值和紫外线防护指数UPF的影响，分析了染色织物的荧光效果、染色牢度和耐水洗性能。结果表明，染色蚕丝织物的K／S值和UPt’指数，随染色时间的延长和氯化钠用量的增加而逐渐升高；随着温度的升高和纯碱用量的增加，其K／S值和UPF指数先升高后降低；染料质量分数为2％～4％（om0时，染色蚕丝织物可满足警示服的荧光要求。当染料质量分数3%（omf），抗紫外线整理剂UV-SUN CEL LIQ5%（omf），纯碱0．5g／L，氯化钠50g／L，在70℃保温60min时，染色织物UPF指数接近120，且耐洗性很好，经30次标准洗涤后，其UPF指数仍超过90。
文摘DNA-binding fluorochromes are often used for vital staining of plant cell nuclei. However, it is not always sure whether the cells after staining still remain in living state. We chose several criteria to estimate the validity of real vital staining for sexual cell nuclei. These were: the cytoplasmic streaming in pollen tubes whose nuclei were stained, the simultaneous visualization of fluo-rochromatic reaction and nucleus staining in isolated generative cells, and the capability of isolated, prestained generative or sperm cells to fuse with other protoplasts. The results confirmed that 4',6-diamidino-2-phenylindole (DAPI), Hoechst 33258 and mithramycin could be used as real vital stains, though their efficiency varied from case to case; among them DAPI showed best effect. The fluorescent vital staining technique offered a useful means fori-dentification and selection of heterokaryons in gametoplast manipulation studies.
