家禽的活样品检查和尸体剖检

检查个体或群体的一般表现并尽量判断哪个器官或系统发病,记录有诊断价值的症状和病变。尸体剖检,就要选择在群体中发病有代表性的禽鸟;如果出现死亡,则发病和死亡的禽鸟都要进行剖检。禽的尸体剖检需从头、消化道、呼吸道、生殖器官及胸腔、腹腔等脏器进行排查,初步判断所患疾病。1活样本检查有些疾病尤其是影响运动的,在活禽是非常容易诊断的。

从活虫体样品中分离虫生真菌初探

１９９２～１９９３年，从广西全境（Ｎ２１°～２６°，Ｅ１０６°～１１１°）各经纬线上采集活虫样品，分离虫生真菌。结果表明，其平均检出率为８．９％，检出率与昆虫种类、作物种类、采样地区及采样时间有关：鳞翅目幼虫样品的检出率高于鞘翅目和半翅目成虫；生长期长的树木上采集的样品检出率高于生长期短的蔬菜、豆类；不同经纬度采集的样品，检出率随经度上升而升高，随纬度的升高而下降；不同时间采集的样品，检出率从高至低依次为５月、４月、３月。

广州市活禽批发市场禽流感病毒污染状况调查

[目的 ]了解广州市活禽批发市场禽流感病毒的污染情况,为广州市制定有针对性的防控措施提供技术支持。[方法 ]2015年2月—2016年1月,按照随机抽样原则,对广州市2个活禽批发市场进行调查,每月采集1次棉拭子样品(共3 090份),并对所采集的样品进行禽流感病毒荧光RT-PCR检测。[结果 ]在该期间内,2个活禽批发市场的样品禽流感总阳性率为52.50%(1 625/3 090);禽流感病毒检出阳性率较高的月份是4月和9—12月,较低的是6—8月;水禽的禽流感感染风险是鸡的1.6倍,环境中存在禽流感病毒的风险是鸡的1.39倍;各月份中,1月、3月、11月和12月环境样品的禽流感病毒阳性率显著高于家禽样品(P<0.01),而6月份家禽样品的禽流感病毒阳性率显著高于环境样品(P<0.01);来自广东省内(广州市除外)的活禽样品禽流感病毒阳性率最高,为49.31%,来自外省市的禽流感通用型阳性率最低,为31.43%(P<0.01)。[结论 ]调查发现,广州市活禽批发市场的禽流感高发期为春夏及秋冬交替季节;活禽批发市场中的水禽和批发市场环境是禽流感病毒流行的关键危险因素。调查结果提示,今后要加强春夏和秋冬交替季节的禽流感防控工作,加强对批发市场水禽的禽流感防控和批发市场环境的清洗、消毒,以切实降低活禽批发市场的禽流感污染水平。

Adsorption of volatile organic compounds on three activated carbon samples:Effect of pore structure

To investigate the influence of the activated carbon pore structure on the adsorption of volatile organic compounds （VOCs）, three commercial activated carbon samples were chosen. The fixed-bed thermostatic adsorption experiments were conducted under certain conditions, where toluene, acetone, and 1, 2-dichloroethane acted as adsorbents. Then, the incidence relation between the experimental results and the activated carbon pore structure was analyzed. After that, the results of the correlation analysis were verified in accordance with fractal theory and adsorption characteristic curve analysis. The results show that the pore diameter gradient is helpful for strengthening the intemal diffusion. Under the same condition, the adsorption of organic gases tends to be selective, and the positions of toluene, acetone and 1, 2-dichloroethane adsorbed on the activated carbon are mainly in the ranges of 1.27-1.49 nm, 0.67-0.84 nm and 1.39-1.75 nm, respectively. The relationship between adsorption capacity and activated carbon pore volume can accurately explain the spreading process of the adsorbents in the activated carbon.

Cloud-point extraction and preconcentration of bisphenol A from water samples

A novel cloud-point extraction （CPE） was successfully used in preconcentration of biphenol A （BPA） from aqueous solutions. Majority of BPA is extracted into the surfactant-rich phase. The parameters affecting the CPE such as concentration of surfactant and electrolyte, equilibration temperature and time and pH of sample solution were investigated. The samples were analyzed by high-performance liquid chromatography with ultraviolet detection. Under the optimized conditions, preconcentration of 10 mL sample gives a preconcentration factor of 11. The limit of detection （LOD） and limit of quantification （LOQ） are 0.1 μg/L and 0.33 μg/L, respectively. The linear range of the proposed method is 0.2-20 μg/L with correlation coefficients greater than 0.998 7 and the spiking recove6es are 97.96%-100.42%. The interference factor was tested and the extraction mechanism was also investigated. Thus, the developed CPE has proven to be an efficient, green, rapid and inexpensive approach for extraction and preconcentration of BPA from water samples.

Application of Neutron Activation Analysis Technique for the Analysis of Soil Samples from Farmlands of Yebrage Hawariat, East Gojjam, Ethiopia

Farmers may not be conscious for their farmland＇s nutrients, soil organic matter, water and air because they simply concerned only for their labor availability and soil fertility losses. The composition and proportion of these components greatly influence soil physical properties, including texture, structure and porosity, the fraction of pore space in a soil. The soil of this farmland must be able to supply adequate amount of plant nutrients, in forms which can be absorbed by the crop, within its lifespan. Deficiencies or imbalances in the supply of any of essential elements can compromise growth, affecting root development, cell division, crop quality, crop yield and resistance to disease and drought. This study was conducted to fill this knowledge gap in order to develop economically vital and environmentally accepted nutrient management strategies for the use of soils in agricultural lands. The objective of this study is to assess the elemental contents and concentration of soil samples collected from farmlands of ＂Yebrage＂ using neutron activation analysis （NAA） techniques regardless of oxidation state, chemical form or physical locations. NAA is used to determine the elemental composition and concentrations present in a soil. The macro/micronutrient and organic matter deficiencies have been verified in agricultural soils through increased use of soil testing and plant analysis. The challenge for agriculture over the coming decades will meet the world＇s increasing demands for food in a sustainable way. Current issues and future challenges point out that as long as agriculture remains a soil based industry, major decreases in productivity likely to be attained ensuring that plants do not have adequate and balanced supply of nutrients.

Oval mucosal opening bloc biopsy after incision and widening by ring thread traction for submucosal tumor

Gastric submucosal tumors(SMTs) less than 2 cm are generally considered benign neoplasms, and endoscopic observation is recommended, but SMTs over 2 cm, 40% of which are gastrointestinal stromal tumors(GISTs), have malignant potential. Although the Japanese Guidelines for GIST recommend partial surgical resection for GIST over 2 cm with malignant potential as well as en bloc large tissue sample to obtain appropriate and large specimens of SMTs, several reports have been published on tissue sampling of SMTs, such as with endoscopic ultrasound sound fine needle aspiration, submucosal tunneling bloc biopsy, and the combination of bite biopsy and endoscopic mucosal resection. Because a simpler, more accurate method is needed for appropriate treatment, we developed oval mucosal opening bloc biopsy after incision and widening by ring thread traction for submucosal tumor(OMOB) approach. OMOB was simple and enabled us to obtain large samples under direct procedure view as well as allowed us to restore to original mucosa.

Behavior of phenol adsorption on thermal modified activated carbon

Adsorption process is acknowledged as an effective option for phenolic wastewater treatment. In this work, the activated carbon （AC） samples after thermal modification were prepared by using muffle furnace. The phenol ad- sorption kinetics and equilibrium measurements were carried out under static conditions at temperature ranging from 25 to 55 ~C. The test results show that the thermal modification can enhance phenol adsorption on AC samples. The porous structure and surface chemistry analyses indicate that the decay in pore morphology and decrease of total oxygen-containing functional groups are found for the thermal modified AC samples. Thus, it can be further inferred that the decrease of total oxygen-containing functional groups on the modified AC sam- ples is the main reason for the enhanced phenol adsorption capacity. For both the raw sample and the optimum modified AC sample at 900 ~C, the pseudo-second order kinetics and Langmuir models are found to fit the exper- imental data very well. The maximum phenol adsorption capacity of the optimum modified AC sample can reach 144,93 mg.g ~ which is higher than that of the raw sample, i.e. 119.53 mg.g 1. Adsorption thermodynamics analysis confirms that the phenol adsorption on the optimum modified AC sample is an exothermic process and mainly via physical adsorption.

Role of Ni(NO_3)_2 in the preparation of a magnetic coal-based activated carbon

The role of Nil（NO3）2 in the preparation of a magnetic activated carbon is reported in this paper. Magnetic coal-based activated carbons （MCAC） were prepared from Taixi anthracite with low ash content in the presence of Ni（NO3）2. The MCAC materials were characterized by a vibrating sample magnetometer （VSM）, X-ray diffraction （XRD）, a scanning electric microscope （SEM）, and by N2 adsorption. The cylindri- cal precursors and derived char were also subjected to thermogravimetric analysis to compare their behavior of weight losses during carbonization. The results show that MCAC has a larger surface area （1074 m21g） and a higher pore volume （0.5792 cm3/g） with enhanced mesopore ratio （by about 10~）. It also has a high saturation magnetization （1.6749 emu/g） and low coercivity （43.26 Oe）, which allows the material to be magnetically separated. The MCAC is easily magnetized because the nickel salt is con- vetted into Ni during carbonization and activation. Metallic Ni has a strong magnetism on account of electrostatic interaction. Added Ni（NO3）2 catalyzes the carbonization and activation process by accelerat- ing burn off of the carbon, which contributes to the development of mesopores and macropores in the activated carbon.

A Comparison between Neutron Activation Analysis and X-Ray Fluorescence Methods in Analyzing the Granite Samples

This study focused on the performance of where elements analysing techniques were used to detect the elements in granite stones. These techniques are NAA （neutron activation analysis） and XRF （X-ray fluorescence）. They were applied to detect the elements in samples which had been chosen from different areas of Pulua Penang in Malaysia collected by geophysics group which helped to describe and identify the elements found in the granite stone that were used in the study procedures to control the analytical results. The integration of both methods has enabled the researcher to determine 40 elements in the samples. The numbers of elements detected by XRF analysis method are 12 elements （Ar, K, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu and Zn）; while, the elements detected by NAA method have three folds of elements with XRF analysis method were 35 elements （Na, AI, Si, K, Ca, Sc,Ti, Mn, Fe, Co, Ga, Ce, As, Br, Rb, Zr, Sb, I, Cs, Ba, La, Nd, Sm, Eu,Tb, Dy, Yb, Lu, Hf, Ta, W, Au, Pa and Np）. Seven common elements were detected in both techniques： K, Sc, Ti, V, Mn, Fe and Co. Si has a higher concentration in NAA technique which is 331.8 ppm. Sc has a lower concentration in XRF technique which is 0.25 ppm. Nd has a lower concentration in NAA technique which is 3.09 - 10-5 ppm. Finally, it is found that the NAA is better to detect the elements than XRF.

Biodeterioration of Building Timbers in the High-Water-Activity Built Environment of Nigeria

Moulds have been reported to destroy volumes of timbers in buildings annually. As a result, timber components within the built environment decline and fail to fulfill their basic requirements. This research focused on the isolation and evaluation of the prevalence and effects of deteriorating moulds in the rain forest and swampy rain forest regions of Nigeria where the water activity is as high as 0.7. To accomplish this, decayed timber samples were aseptically collected on buildings from six strategic locations. The samples were serially diluted and inoculated onto Sabouraud Dextrose Agar medium in Petal dishes. The Petri dishes were incubated for 72 h at 30 ~C. Thereafter, moulds were isolated through visual and microscopic observations. The commonly encountered moulds were evaluated and analyzed. It was observed that, prevalence of moulds on buildings used for non residential purpose were higher. There was no significant difference between the prevalence on the components located inside the building and those outside the building. Ceiba pentandra exhibited highest degradation while Masonia altissima resisted most. The most deteriorating moulds were Aspergillus, Mucor, Rhizopus and Gliocladium. The deteriorations of Ceiba pentandra, Afzelia africana, Lophira alata, Anogessus leocarpus and Gossweilerodendron balsamiferum timbers under Aspergillus attack were projected.

Structural Aerospace Composite/Metallic Samples under Fatigue Loads： Experimental Activity on Three Case Studies

The use of composite materials in aerospace engineering is ever increasing. Properties such as low weight, high performance, high stiffness and the ability for it to be tailored specifically for different structural uses has increased its importance in recent years. Specific experimental/numerical activities were developed in the last decade at the aerospace department of Polito and are under consideration in recent years regarding the analysis of fatigue behavior of composite/metallic structural samples under cyclic loading condition. A review of the main significant results connected to three case-studies is presented in the paper. The first is related to the definition and analysis of innovative joint for composite high aspect ratio aircraft structures. The modular design oriented to limit component dimensions for transportability requires the presence of a removable joints able to overcome the typical low bearing failure level of composite pin-loaded joint under static and fatigue loading distribution according to airworthiness requirements. The static experiments demonstrate the consistency of the proposed advanced joint configuration that postpones the joint failure to high load level according to the structural requirements. The cyclic loading phase takes as reference point the failure load of the initial configuration and defines a loading cycle with a maximum higher than the failure load of the initial configuration. The fatigue behavior of the advanced joint also demonstrates a substantial consistency with expected duration of 1 e6 cycles as required by the structural design for operating reasons. Specific indication on fatigue limit with respect to critical amplitude is reported. The second is related to the presence of specific damaged situation in thin-walled fiat/stiffened composite plate. Stiffened configurations are frequently used to increase buckling load level. Unexpected events on solid/stiffened composite panels can introduce a certain level of damage, typically delamination, that can cause reduction in buckling design level and reduction in global strength. The presence of cyclic load and fatigue effect can have an important consequence on damage propagation and structural integrity. The damaged area determined by the skin-stiffener de-bonding of a certain dimension is investigated under static compression and cyclic compression. Local buckling of damaged area is determined and pointed out by tests in uniaxial compression. The experimental static behavior points out the presence of a snap-effect during loading and un-loading. Fatigue loading configuration is applied in the range of post-critical local configuration considered as the most effective situation. Preliminary fatigue results are presented and discussed. The third is related to preliminary investigation on the effect of fatigue life reduction of 2024 AI alloy in corrosive （exfoliation） environment. The effect of corrosion is taken into consideration introducing specific concentration factors into the life estimation relationship. Differences between fatigue in prior corroded specimens and fatigue in presence of corrosive environment are emphasized. No crack propagation is considered. Related concentration factors are derived and compared by the few experimental results in order to define some guidelines for design process and to improve aircraft better evaluation of component structural integrity in operative situations. A preliminary approach is presented in the paper in order to correctly identify the contribution of corrosive environment in coupled fatigue loading phase. The results are discussed and future improvements are suggested.

数字全息显微定量相位成像的实验研究

建立了一套预放大式数字全息显微成像系统,通过对样品进行显微放大,实现了高分辨率的定量相位成像;并通过计算机控制相机自动曝光记录序列的数字全息图实现了动态相位成像。用标准样品验证了系统测量的准确性;以活体洋葱表皮细胞和血红细胞为样品,获得了清晰的定量相位像;以置于水环境的草履虫为样品,实现了动态成像。实验结果表明建立的系统可以实现高分辨率的动态定量相位成像,可以应用于生物活体样品的显微研究。

Hydrothermal activities and seawater acidification in the Late Devonian F-F transition:Evidence from geochemistry of rare earth elements

Rare-earth dements （REE） in limestones were measured by ICP-MS from the Yangdi section across the Frasnian-Famennian （F-F） transition in Guilin, Guangxi of South China. The average EREE contents of measured samples are 33.32 ppm. The average LREE/HREE ratio is 6.35. Data show LREE-enriched REE distribution pattern in the Yangdi section. Most of samples are characterized by positive Eu anomaly with average of 1.13, and there are two maxima of Eu anomaly just below the F-F boundary. Almost all analytical samples show obvious tetrad effects. Remarkable M-shaped tetrad effects are observed in these samples with positive Eu anomaly. The characters of REE and some relative elements （Fe, Mn, Al, etc.） indicate that hydro- thermal activities occurred and resulted in seawater acidification in Upper and Lower Kellwasser horizons of the Late Devonian. The changes of marine environment caused by hydrothermal activities, such as seawater acidification, heavy metal pollution, and eutrophication, would destroy the neritic ecosystem and finally result in the F-F mass extinction.

A novel purification technique for noble gas isotope analyses of authigenic minerals

Noble and active gases are released from geological samples during gas extraction for noble gas isotope analyses. The active gases should be removed before inletting to mass spectrometers for the analyses. The normal noble gas preparation systems can clean up most geological samples. However, authigenic minerals from sedimentary rocks in oil/gas fields contain organic matter, which cannot be cleaned up by the normal preparation systems and thus influence the noble gas analyses. We introduce a novel gas purification system （PRC patent No. ZL201320117751.2）, which includes several reversible purification pumps with different absorbing and degassing temperatures. It can well clean up water steam, carbon dioxide and organic gas- es. Mica minerals are often used for 40Ar/39Ar dating. A muscovite sample （2082MS） which could not be cleaned up by the normal preparation system with two SAES NP10 getters, becomes the test sample for a comparative experiment in this study. The experiment is assigned into 4 sections with the organic gas removal system （OGRS） ＂Closed/Opened＂ in turn. When the OGRS is closed only with two NP10 getters for purification, the 40At intensities increase in curves with inlet time because of impurities, the 40Ar/39Ar dating results yield age errors about ＋2%-±1% （20-）. When the OGRS is opened for purification, in contrast, the 40Ar intensities decrease linearly with inlet time. This indicates that the gases have been cleaned up effectively, and the 40Ar/39Ar results yield ages with errors in ±0.4%. The OGRS is very helpful to obtain high-quality analysis data.

Concentrations and distributions of Dechlorane Plus in environmental samples around a Dechlorane Plus manufacturing plant in East China

A comprehensive investigation into the occur- rence of Dechlorane Plus （DP） in environmental samples around a DP manufacturing plant in East China was per- formed. The total syn-DP and anti-DP concentrations found in the soil, sediment, active air, and passive air samples were 0.50-2,315 pg/g dry weight （dw）, 0.32-20.5 ng/g dw, 5.52-3,332 pg/m^3, and 1.00-4,560 pg/m^3, respectively. There were consistent trends in the spatial distributions of DP in the soil and air samples, with the DP concentrations de- creasing dramatically as the distance from the plant in- creased. The mean anti-DP isomer fractional abundances （fanti） in the soil, sediment, active air, and passive air samples were 0.67, 0.68, 0.70, and 0.64, respectively. These fanti values were consistent with the fanti value for the technical DP produced in the DP manufacturing plant （0.68）, meaning that the DP had been released during the local DP production activities.