Peroxisome proliferator-activated receptors （PPARs） are ligand-activated transcription factors known to play a pivotal role in regulations of metabolism. In order to yield soluble ligand binding domain of PPARδ （P...Peroxisome proliferator-activated receptors （PPARs） are ligand-activated transcription factors known to play a pivotal role in regulations of metabolism. In order to yield soluble ligand binding domain of PPARδ （PPARδLBD） for screening ligands, the cDNA was amplified using total RNA from HepG2 cells by RT-PCR. Then the enzyme-digested product was inserted . downstream of the malE gene in the vectorpMAL-p2x, which encoded maltose-binding protein （MBP）, resulting in the expression of an MBP-PPARδLBD fusion protein. The recombinant plasmid was transformed into E. coli TBI that was cultured shakily at 30 ℃, 200 r/min and induced by 0.4 mmol/L IPTG for 6 h. The cells were harvested by centrifugation and broken by sonication. The expressed fusion protein was soluble and accounted for 0.31 of the total protein in the supernatant. Western blot analysis showed that the expressed MBP-PPARδLBD could bind to anti-MBP-antibody. The MBP-PPARδLBD fusion protein of 77 kDa and the PPARδLBD protein of 34 kDa were obtained by amylose-resin affinity chromatography without or with digestion of Factor Xa. They were both homogeneity, judged by SDS-PAGE. The recombinant MBP-PPARδLBD and PPARδLBD protein with high purity is obtained, which provides the necessary material for screening and researching PPARδ ligands.展开更多
This paper presents a brief review of distant biological communication phenomena, information fields explained as a transduction of information without energy displacement, experimental requirements for testing this h...This paper presents a brief review of distant biological communication phenomena, information fields explained as a transduction of information without energy displacement, experimental requirements for testing this hypothesis with human beings using electrophotonic analysis, oxymetry and electromagnetic shielding. Finally, authors present preliminary results and future work on this new field of interdisciplinary research.展开更多
Philosophy of science has arisen as alternative to epistemology, because scientific development wanted another kind of explanation than traditional epistemological one. The latter kind of explanation is theoretically ...Philosophy of science has arisen as alternative to epistemology, because scientific development wanted another kind of explanation than traditional epistemological one. The latter kind of explanation is theoretically loaded and based on latent ontological assumptions. Epistemology offers science a ＂road map＂ for researcher＇s thinking. Thus epistemology knows what science should be, and philosophy of science take science for granted--existing as an empirical fact. Philosophy of science had always been a reflective and critical discipline that didn＇t want any a-priory knowledge. The main distinction between these fields of knowledge is as follows： epistemology is theoretical and projective kind of knowledge whereas philosophy of science is a non-theoretical, or better, a ＂post-theoretical＂ discipline. All facts and statements arising from philosophy of science lie within the heterogeneous space, where there is continuous transit of pre-theoretical, theoretical and post-theoretical versions of knowledge. The inevitability of conservation and utilization of human thought products is recognized, which is supplemented by the need to study the impact of theoretical thinking upon social practices.展开更多
Natural products are often secondary metabolites in living organisms with a wide variety of biological activities. The diversification of their structures, aiming to the search for biologically active small molecules ...Natural products are often secondary metabolites in living organisms with a wide variety of biological activities. The diversification of their structures, aiming to the search for biologically active small molecules by expanding chemical and functional spaces, is a major area of current interest in synthetic chemistry. However, developing synthetic accessibility and efficiency often faces challenges associated with structural complexity. Synthetic biology has recently emerged and is promising to accomplish complex molecules; by contrast, the application to structural diversification of natural products relies on the understanding, development and utilization of compatible biosynthetic machinery. Here, we review the strategies primarily concerning the artificial evolution of microbial natural products whose biosynthesis features template enzymology, including ribosomaUy synthesized and post-translationally modified peptides as well as the assembly line-resultant polyketides, non-ribosomal peptides and hybrids. The establishment of these approaches largely facilitates the expansion of the molecular diversity and utility through bioengineering at different stages/levels of biosynthetic pathways.展开更多
The stimuli-responsive nanomaterials are gaining more and more interest in the biological field,including cell imaging and biosensing etc. Nanomaterials in response to the bio-relevant stimuli(i.e., p H, enzymes and o...The stimuli-responsive nanomaterials are gaining more and more interest in the biological field,including cell imaging and biosensing etc. Nanomaterials in response to the bio-relevant stimuli(i.e., p H, enzymes and other bioactive molecules) can be utilized to enhance imaging(i.e., optical imaging, MRI, and multi-mode imaging) sensitivity via disease site-specific delivery and controlled release, which helps to diagnose cancer at an early stage or to monitor progression during treatment. In the triggered responsive process, smart nanomaterials undergo changes in physiochemical properties that can cause cytotoxicity or influence on cell functions due to the interactions between nanomaterials and cells. In order to promote the design and fabrication of effective platforms for therapeutics and diagnostics, special attention should be paid to these effects. By taking the advantages of intracellular stimuli, the controlled self-assembly in living cells can be achieved, which has been used for various in situ detections and insights into biological self-assembly. In this review, the recent advances in cell imaging, cytotoxicity and self-assembly of intracellular stimuli-responsive nanomaterials are summarized. Some principles for the further design and applications of intracellular stimuli-responsive nanomaterials and future perspectives are discussed.展开更多
基金Supported by the National Natural Science Foundation of China (30572353)
文摘Peroxisome proliferator-activated receptors （PPARs） are ligand-activated transcription factors known to play a pivotal role in regulations of metabolism. In order to yield soluble ligand binding domain of PPARδ （PPARδLBD） for screening ligands, the cDNA was amplified using total RNA from HepG2 cells by RT-PCR. Then the enzyme-digested product was inserted . downstream of the malE gene in the vectorpMAL-p2x, which encoded maltose-binding protein （MBP）, resulting in the expression of an MBP-PPARδLBD fusion protein. The recombinant plasmid was transformed into E. coli TBI that was cultured shakily at 30 ℃, 200 r/min and induced by 0.4 mmol/L IPTG for 6 h. The cells were harvested by centrifugation and broken by sonication. The expressed fusion protein was soluble and accounted for 0.31 of the total protein in the supernatant. Western blot analysis showed that the expressed MBP-PPARδLBD could bind to anti-MBP-antibody. The MBP-PPARδLBD fusion protein of 77 kDa and the PPARδLBD protein of 34 kDa were obtained by amylose-resin affinity chromatography without or with digestion of Factor Xa. They were both homogeneity, judged by SDS-PAGE. The recombinant MBP-PPARδLBD and PPARδLBD protein with high purity is obtained, which provides the necessary material for screening and researching PPARδ ligands.
文摘This paper presents a brief review of distant biological communication phenomena, information fields explained as a transduction of information without energy displacement, experimental requirements for testing this hypothesis with human beings using electrophotonic analysis, oxymetry and electromagnetic shielding. Finally, authors present preliminary results and future work on this new field of interdisciplinary research.
文摘Philosophy of science has arisen as alternative to epistemology, because scientific development wanted another kind of explanation than traditional epistemological one. The latter kind of explanation is theoretically loaded and based on latent ontological assumptions. Epistemology offers science a ＂road map＂ for researcher＇s thinking. Thus epistemology knows what science should be, and philosophy of science take science for granted--existing as an empirical fact. Philosophy of science had always been a reflective and critical discipline that didn＇t want any a-priory knowledge. The main distinction between these fields of knowledge is as follows： epistemology is theoretical and projective kind of knowledge whereas philosophy of science is a non-theoretical, or better, a ＂post-theoretical＂ discipline. All facts and statements arising from philosophy of science lie within the heterogeneous space, where there is continuous transit of pre-theoretical, theoretical and post-theoretical versions of knowledge. The inevitability of conservation and utilization of human thought products is recognized, which is supplemented by the need to study the impact of theoretical thinking upon social practices.
基金supported by the National Natural Science Foundation of China (81402831, 21520102004, 31430005, 21472231)Science and Technology Commission of Shanghai Municipality (Shanghai, China) (14JC1407700, 15JC1400400) of China
文摘Natural products are often secondary metabolites in living organisms with a wide variety of biological activities. The diversification of their structures, aiming to the search for biologically active small molecules by expanding chemical and functional spaces, is a major area of current interest in synthetic chemistry. However, developing synthetic accessibility and efficiency often faces challenges associated with structural complexity. Synthetic biology has recently emerged and is promising to accomplish complex molecules; by contrast, the application to structural diversification of natural products relies on the understanding, development and utilization of compatible biosynthetic machinery. Here, we review the strategies primarily concerning the artificial evolution of microbial natural products whose biosynthesis features template enzymology, including ribosomaUy synthesized and post-translationally modified peptides as well as the assembly line-resultant polyketides, non-ribosomal peptides and hybrids. The establishment of these approaches largely facilitates the expansion of the molecular diversity and utility through bioengineering at different stages/levels of biosynthetic pathways.
基金supported by the National Natural Science Foundation of China(51120135001)Ph.D.Programs Foundation of Ministry of Education of China(20110101130005)
文摘The stimuli-responsive nanomaterials are gaining more and more interest in the biological field,including cell imaging and biosensing etc. Nanomaterials in response to the bio-relevant stimuli(i.e., p H, enzymes and other bioactive molecules) can be utilized to enhance imaging(i.e., optical imaging, MRI, and multi-mode imaging) sensitivity via disease site-specific delivery and controlled release, which helps to diagnose cancer at an early stage or to monitor progression during treatment. In the triggered responsive process, smart nanomaterials undergo changes in physiochemical properties that can cause cytotoxicity or influence on cell functions due to the interactions between nanomaterials and cells. In order to promote the design and fabrication of effective platforms for therapeutics and diagnostics, special attention should be paid to these effects. By taking the advantages of intracellular stimuli, the controlled self-assembly in living cells can be achieved, which has been used for various in situ detections and insights into biological self-assembly. In this review, the recent advances in cell imaging, cytotoxicity and self-assembly of intracellular stimuli-responsive nanomaterials are summarized. Some principles for the further design and applications of intracellular stimuli-responsive nanomaterials and future perspectives are discussed.
