切应力和溶血磷脂酰胆碱对内皮细胞表面黏附分子表达的影响

在体内 ,内皮细胞的功能不仅受化学因子的调节 ,而且还受力学因素的影响。为探讨流体切应力和溶血磷脂酰胆碱 ( L ysophosphatidylcholine,L yso- PC)的双重作用对培养的人脐静脉内皮细胞 ( Hum an um bilical veinendothelial cells,HU VECs)表面黏附分子 ICAM- 1、VCAM- 1、E- selectin表达的影响 ,采用流式细胞仪技术检测了L yso- PC( 3 0 μg/m l)和流体切应力 ( 2 .2 3、4.2 0 dyne/cm2 )的协同作用下内皮细胞黏附分子表达的变化。结果显示 :在受剪切作用之前 ,用 L yso- PC孵育激活内皮细胞 ,或预先剪切后再用 L yso- PC孵育 ,内皮细胞的 ICAM- 1和VCAM- 1表达与两种刺激同时作用相比 ,显著增加 ( P<0 .0 5 ) ;切应力或 L yso- PC的单独作用 ,以及两种刺激同时存在对 HU VEC的 E- selectin表达无显著影响。而在受剪切作用之前 ,用 L yso- PC孵育激活内皮细胞 ,或预先剪切后再用 L yso- PC孵育 ,内皮细胞的 E- selectin表达与两种刺激同时作用相比 ,显著增加 ( P<0 .0 5 )。结论认为 :即使在不利于细胞黏附的力学环境中 ,流体切应力与 L yso- PC的协同作用 。

Turbulent boundary layers and hydrodynamic flow analysis of nanofluids over a plate

A numerical analysis of the log-law behavior for the turbulent boundary layer of a wall-bounded flow is performed over a flat plate immersed in three nanofluids(Zn O-water,SiO_(2)-water,TiO_(2)-water).Numerical simulations using CFD code are employed to investigate the boundary layer and the hydrodynamic flow.To validate the current numerical model,measurement points from published works were used,and the compared results were in good compliance.Simulations were carried out for the velocity series of 0.04,0.4 and 4 m/s and nanoparticle concentrations0.1% and 5%.The influence of nanoparticles’ concentration on velocity,temperature profiles,wall shear stress,and turbulent intensity was investigated.The obtained results showed that the viscous sub-layer,the buffer layer,and the loglaw layer along the potential-flow layer could be analyzed based on their curving quality in the regions which have just a single wall distance.It was seen that the viscous sub-layer is the biggest area in comparison with other areas.Alternatively,the section where the temperature changes considerably correspond to the thermal boundary layer’s thickness goes a downward trend when the velocity decreases.The thermal boundary layer gets deep away from the leading edge.However,a rise in the volume fraction of nanoparticles indicated a minor impact on the shear stress developed in the wall.In all cases,the thickness of the boundary layer undergoes a downward trend as the velocity increases,whereas increasing the nanoparticle concentrations would enhance the thickness.More precisely,the log layer is closed with log law,and it is minimal between Y^(+)=50 and Y^(+)=95.The temperature for nanoparticle concentration φ=5%is higher than that for φ=0.1%,in boundary layers,for all studied nanofluids.However,it is established that the behavior is inverted from the value of Y^(+)=1 and the temperature for φ =0.1% is more important than the case of φ =5%.For turbulence intensity peak,this peak exists at Y^(+)=100 for v=4 m/s,Y^(+)=10 for v=0.4 m/s and Y^(+)=8 for v=0.04 m/s.

Experimental and CFD Studies on the Performance of Microfiltration Enhanced by a Turbulence Promoter

This paper reports experimental and computational fluid dynamics(CFD) studies on the performance of microfiltration enhanced by a helical screw insert.The experimental results show that the use of turbulence pro-moter can improve the permeate flux of membrane in the crossflow microfiltration of calcium carbonate suspension,and flux improvement efficiency is strongly influenced by operation conditions.The energy consumption analysis indicates that the enhanced membrane system is more energy saving at higher feed concentrations.To explore the intrinsic mechanism of flux enhancement by a helical screw insert,three-dimensional CFD simulation of fluid flow was implemented.It reveals that hydrodynamic characteristics of fluid flow inside the channel are entirely changed by the turbulence promoter.The rotational flow pattern increases the scouring effect on the tube wall,reducing the particle deposition on the membrane surface.The absence of stagnant regions and high wall shear stress are respon-sible for the enhanced filtration performance.No secondary flow is generated in the channel,owing to the streamline shape of helical screw insert,so that the enhanced performance is achieved at relatively low energy consumption.

Effects of Bulbous Bow on Cross-Flow Vortex Structures Around a Streamlined Submersible Body at Intermediate Pitch Maneuver： A Numerical Investigation

A flow field around a streamlined body at an intermediate angle of incidence is dominated by cross-flow separation and vortical flow fields. The separated flow leads to a pair of vortices on the leeside of the body; therefore, it is essential to accurately determine this pair and estimate its size and location. This study utilizes the element-based finite volume method based on RANS equations to compute a 3D axisymmetric flow around a SUBOFF bare submarined hull. Cross-flow vortex structures are then numerically simulated and compared for a submarine with SUBOFF and DRDC STR bows. Computed results of pressure and shear stress distribution on the hull surface and the strength and locations of the vortex structures are presented at an intermediate incidence angle of 20°. A wind tunnel experiment is also conducted to experimentally visualize the vortex structures and measure their core locations. These experimental results are compared with the numerical data, and a good agreement is found.

Influence of Collective Boulder Array on the Surrounding Time-averaged and Turbulent Flow Fields

Arrays of large immobile boulders,which are often encountered in steep mountain streams,affect the timing and magnitude of sediment transport events through their interactions with the approach flow.Despite their importance in the quantification of the bedload rate,the collective influence of a boulder array on the approach timeaveraged and turbulent flow field has to date been overlooked.The overarching objective is,thus,to assess the collective effects of a boulder array on the time-averaged and turbulent flow fields surrounding an individual boulder within the array,placing particular emphasis on highlighting the bed shear stress spatial variability.The objective of this study is pursued by resolving and comparing the timeaveraged and turbulent flow fields developing around a boulder,with and without an array of isolated boulders being present.The results show that the effects of an individual boulder on the time-averaged streamwise velocity and turbulence intensity were limited to the boulder's immediate vicinity in the streamwise(x/d c < 2-3) and vertical(z/d c < 1) directions.Outside of the boulder's immediate vicinity,the time-averaged streamwise velocity was found to be globally decelerated.This global deceleration was attributed to the form drag generated collectively by the boulder array.More importantly,the boulder array reduced the applied shear stress exerted on theindividual boulders found within the array,by absorbing a portion of the total applied shear.Furthermore,the array was found to have a "homogenizing" effect on the near-bed turbulence thus significantly reducing the turbulence intensity in the near-bed region.The findings of this study suggest that the collective boulder array bears a portion of the total applied bed shear stress as form drag,hence reducing the available bed shear stress for transporting incoming mobile sediment.Thus,the effects of the boulder array should not be ignored in sediment transport predictions.These effects are encapsulated in this study by Equation(6).

Possible effect of fluid shear stress on osteoclastogenesis

Bone remodeling is performed under the joint action of osteoblasts and osteoclasts. Since the effect of osteoclasts has been gradually recognized on bone and joint diseases, targeted researches toward osteoclasts have become a hot research field. This article reviews the relevant medical literature concerning the possible effects of the fluid shear stress (FSS) on the osteoclastogenesis chiefly from the aspects of RANKL-RANK-OPG system, the macrophage colony-stimulating factor (M-CSF), and calcitonin receptor (CTR). On the basis of the changes of the expression of osteoclastic activities, it is suggested that FSS is a potent, important regulator of bone metabolism.

Enhancement in Magnetorheological Effect of Magnetorheological Elastomers by Surface Modification of Iron Particles

In order to obtain magnetorheological （MR） elastomers with high magnetorheological effect, a family of anisotropic rubber-based MR elastomers was developed using a new form of chemical modification. Three different kinds of surfactants, i.e. anionic, nonionic and compound surfactants, were employed separately to modify iron particles. The MR effect was evaluated by measuring the dynamic shear modulus of MR elastomer with a magneto-combined dynamic mechanical analyzer. Results show that the relative MR effect can be up to 188% when the iron particles are modified with 15% Span 80. Besides the surface activity of Span 80, however, such high modifying effect is partly due to the plasticizing effect of Span 80. Compared with the single surfactant, the superior surface activity of compound surfactant makes the relative MR effect reach 77% at a low content of 0.4%. Scanning electron microscope observation shows that the modification of compound surfactant results in perfect compatibility between particles and rubber matrix and special self-assembled structure of particles. Such special structure has been proved beneficial to the improvement of the relative MR effect.

A computational analysis of the impact of mass transport and shear on three-dimensional stem cell cultures in perfused micro-bioreactors

In this study, Computational Fluid Dynamics(CFD) is used to investigate and compare the impact of bioreactor parameters(such as its geometry, medium flow-rate, scaffold configuration) on the local transport phenomena and, hence, their impact on human mesenchymal stem cell(hM SC) expansion. The geometric characteristics of the TissueFlex174;(Zyoxel Limited, Oxford, UK) microbioreactor were considered to set up a virtual bioreactor containing alginate(in both slab and bead configuration) scaffolds. The bioreactor and scaffolds were seeded with cells that were modelled as glucose consuming entities. The widely used glucose medium, Dulbecco's Modified Eagle Medium(DMEM), supplied at two inlet flow rates of 25 and 100 μl·h^(-1), was modelled as the fluid phase inside the bioreactors. The investigation, based on applying dimensional analysis to this problem, as well as on detailed three-dimensional transient CFD results, revealed that the default bioreactor design and boundary conditions led to internal and external glucose transport, as well as shear stresses, that are conducive to h MSC growth and expansion. Furthermore, results indicated that the ‘top-inout' design(as opposed to its symmetric counterpart) led to higher shear stress for the same media inlet rate(25 μl·h^(-1)), a feature that can be easily exploited to induce shear-dependent differentiation. These findings further confirm the suitability of CFD as a robust design tool.

Temperature rise and flow of Zr-based bulk metallic glasses under high shearing stress

Deformation of the bulk metallic glasses (BMGs) and the creation and propagation of the shear bands are closely interconnected.Shearing force was loaded on Zr 41.2 Ti 13.8 Cu 12.5 Ni 10.0 Be 22.5 (Vit.1) BMGs by cutting during the turning of the BMG rod.The temperature rise of alloy on the shear bands was calculated and the result showed that it could reach the temperature of the super-cooled liquid zone or exceed the melting point.The temperature rise caused viscous fluid flow and brought about the deformation of BMGs.This suggested that the deformation of BMGs was derived,at least to some extent,from the adiabatic shear temperature rise.

Blood flow of Carreau fluid in a tapered artery with mixed convection

This research is concerned with the mathematical modeling and analysis of blood flow in a tapered artery with stenosis. The analysis has been carried out in the presence of heat and mass transfer. Constitutive equation of Carreau fluid has been invoked in the mathematical formulation. The representation of blood flow is considered through an axially non-symmetrical but radially symmetric stenosis. Symmetry of the distribution of the wall, shearing stress and resistive impectartce and their growth with the developirtg stenosis is given due attention. Solutions have been obtained for the velocity, temperature, concentration, resistance impedance, wall shear stress and shearing stress at the stenosis throat. Graphical illustrations associated with the tapered arteries namely converging, diverging and non-tapered arteries are examined for different parameters of interest. Streamlines have been plotted and discussed.

Mixed convection analysis for blood flow through arteries on Williamson fluid model

In this paper, the blood flow through a tapered artery with a stenosis by considering axially non-symmetric but radially symmetric mild stenosis on blood flow characteris- tics is analyzed, assuming the flow is steady and blood is treated as Williamson fluid. The effects of mixed convection heat and mass transfer are also carried out. Perturbation solutions have been calculated for velocity, temperature, concentration, resistance impedance, wall shear stress and shearing stress at the stenosis throat. The graphical results of different types of tapered arteries （i.e. converging tapering, diverging tapering, non-tapered artery） have been examined for different parameters of interest. Streamlines have been plotted at the end of the paper.

PRESSURE FLOW OF A SECOND GRADE FLUID THROUGH A CHANNEL OF VARYING WIDTH WITH APPLICATION TO STENOSED ARTERY

Analytical solutions are obtained for steady flow of an incompressible second grade fluid in an axisymmetric channel of varying width. Three approximate methods are used depending upon three different geometrical configuration. The results obtained are applied to study the flow of a second grade fluid through a smooth constriction. To understand the flow behavior near stenosis, resistance to the flow, shear stress at the wall and stress at the stenosis throat are calculated. The results obtained are numerically evaluated for different values of dimensionless non-Newtonian parameters λ1 and λ2 and maximum height of the stenosis δm. It is observed that as we increase the value of these parameters the resistance to the flow, wall shear stress and stress at the stenosis throat increase.

Mathematical analysis of Phan-Thien-Tanner fluid model for blood in arteries

In the present paper, we have studied the blood flow through tapered artery with a stenosis. The non-Newtonian nature of blood in small arteries is analyzed mathematically by considering the blood as Phan-Thien-Tanner fluid. The representation for the blood flow is through an axially non-symmetrical but radially symmetric stenosis. Symmetry of the distribution of the wall shearing stress and resistive impedance and their growth with the developing stenosis is another important feature of our analysis. Exact solutions have been evaluated for velocity, resistance impedance, wall shear stress and shearing stress at the stenosis throat. The graphical results of different type of tapered arteries （i.e. converging tapering, diverging tapering, non-tapered artery） have been examined for different narameters of interest.