A small scale isotropic mass transfer model was developed for the local liquid side mass transfer coefficients in gas-liquid-solid three-phase flow airlift loop reactor for Newtonian and non-Newtonian fluids. It is ba...A small scale isotropic mass transfer model was developed for the local liquid side mass transfer coefficients in gas-liquid-solid three-phase flow airlift loop reactor for Newtonian and non-Newtonian fluids. It is based onHigbie's penetration theory and Kolmogoroff's theory of isotropic turbulence withwhere ε1 is local rate of energy dissipation, Af is the local microscale, η1 is the local Kolmogoroff scale and D is the diffusion coefficient. The capability of the proposed model is discussed in the light of experimental data obtained from 12 L gas-liquid-solid three-phase flow airlift loop reactor using Newtonian and non-Newtonian fluids. Good agreement with the experimental data was obtained over a wide range of conditions suggesting a general applicability of the proposed model.展开更多
AIM： Hydrodynamics based transfection （HBT）, the injection of a large volume of naked plasmid DNA in a short time is a relatively simple, efficient and safe method for in vivo transfection of liver cells. Though us...AIM： Hydrodynamics based transfection （HBT）, the injection of a large volume of naked plasmid DNA in a short time is a relatively simple, efficient and safe method for in vivo transfection of liver cells. Though used for quite some time, the mechanism of gene transfection has not yet been elucidated. METHODS： A lucJferase encoding plasmid was injected using the hydrodynamics based procedure into normal and thioacetamide-induced fibrotic Sprague Dawley rats. Scanning and transmission electron microscopy images were taken. The consequence of a dual injection of Ringer solution and luciferase pDNA was followed. Halofuginone, an anti collagen type I inhibitor was used to reduce ECM load in fibrotic rats prior to the hydrodynamic injection. RESULTS： Large endothelial gaps formed as soon as 10＇ following hydrodynamic injection; these gradually returned to normal 10 d post injection. Hydrodynamic administration of Ringer 10 or 30 m prior to moderate injection of plasmid did not result in efficient transfection suggesting that endothelial gaps by themselves are not sufficient for gene expression. Gene transfection following hydrodynamic injection in thioacetamide induced fibrotic rats was diminished coinciding with the level of fibrosis. Halofuginone, a specific collagen type I inhibitor, alleviated this effect. CONCLUSION： The hydrodynamic pressure formed following HBT results in the formation of large endothelial gaps. These gaps, though important in the transfer of DNA molecules from the blood to the space of Disse are not enough to provide the appropriate conditions for hepatocyte transfection. Hydrodynamics based injection is applicable in fibrotic rats provided that ECM load is reduced.展开更多
An experimental investigation is carded out to study fluid flow and heat transfer characteristics on the endwall fitted with arrays （ 7 × 7 ） of short rectangular plate fins of different pattern （co-angular an...An experimental investigation is carded out to study fluid flow and heat transfer characteristics on the endwall fitted with arrays （ 7 × 7 ） of short rectangular plate fins of different pattern （co-angular and zigzag） for different pitch ratio. Experiments were conducted in a rectangular duct of 50 mm height for an air flow of Reynolds number ranged from 18750 to 62500 based on the equivalent diameter and air velocity of the duct. Infrared image analysis technique was employed to make clear the characteristics of local heat transfer coefficients on fin base, endwall and overall surface. Flow pattern around the short rectangular plates were visualized by inducing fluorescent dye in a water channel and longitudinal vortices were observed. Increasing the distance between plates in flow direction causes heat transfer enhancement for co-angular pattern, while decreasing the distance causes heat transfer enhancement for zigzag pattern. Zigzag pattern with pitch ratio 2 is found to be more effective in heat transfer enhancement than any other cases investigated.展开更多
基金the National Natural Science Foundation of China (No. 20176040, No. 20336030 and No. 90206001).
文摘A small scale isotropic mass transfer model was developed for the local liquid side mass transfer coefficients in gas-liquid-solid three-phase flow airlift loop reactor for Newtonian and non-Newtonian fluids. It is based onHigbie's penetration theory and Kolmogoroff's theory of isotropic turbulence withwhere ε1 is local rate of energy dissipation, Af is the local microscale, η1 is the local Kolmogoroff scale and D is the diffusion coefficient. The capability of the proposed model is discussed in the light of experimental data obtained from 12 L gas-liquid-solid three-phase flow airlift loop reactor using Newtonian and non-Newtonian fluids. Good agreement with the experimental data was obtained over a wide range of conditions suggesting a general applicability of the proposed model.
基金Supported by the Israel Science Foundation, No. 537/01, the C. Rosenblantt Cancer Research Fund and the Rappaport Institute Fund
文摘AIM： Hydrodynamics based transfection （HBT）, the injection of a large volume of naked plasmid DNA in a short time is a relatively simple, efficient and safe method for in vivo transfection of liver cells. Though used for quite some time, the mechanism of gene transfection has not yet been elucidated. METHODS： A lucJferase encoding plasmid was injected using the hydrodynamics based procedure into normal and thioacetamide-induced fibrotic Sprague Dawley rats. Scanning and transmission electron microscopy images were taken. The consequence of a dual injection of Ringer solution and luciferase pDNA was followed. Halofuginone, an anti collagen type I inhibitor was used to reduce ECM load in fibrotic rats prior to the hydrodynamic injection. RESULTS： Large endothelial gaps formed as soon as 10＇ following hydrodynamic injection; these gradually returned to normal 10 d post injection. Hydrodynamic administration of Ringer 10 or 30 m prior to moderate injection of plasmid did not result in efficient transfection suggesting that endothelial gaps by themselves are not sufficient for gene expression. Gene transfection following hydrodynamic injection in thioacetamide induced fibrotic rats was diminished coinciding with the level of fibrosis. Halofuginone, a specific collagen type I inhibitor, alleviated this effect. CONCLUSION： The hydrodynamic pressure formed following HBT results in the formation of large endothelial gaps. These gaps, though important in the transfer of DNA molecules from the blood to the space of Disse are not enough to provide the appropriate conditions for hepatocyte transfection. Hydrodynamics based injection is applicable in fibrotic rats provided that ECM load is reduced.
文摘An experimental investigation is carded out to study fluid flow and heat transfer characteristics on the endwall fitted with arrays （ 7 × 7 ） of short rectangular plate fins of different pattern （co-angular and zigzag） for different pitch ratio. Experiments were conducted in a rectangular duct of 50 mm height for an air flow of Reynolds number ranged from 18750 to 62500 based on the equivalent diameter and air velocity of the duct. Infrared image analysis technique was employed to make clear the characteristics of local heat transfer coefficients on fin base, endwall and overall surface. Flow pattern around the short rectangular plates were visualized by inducing fluorescent dye in a water channel and longitudinal vortices were observed. Increasing the distance between plates in flow direction causes heat transfer enhancement for co-angular pattern, while decreasing the distance causes heat transfer enhancement for zigzag pattern. Zigzag pattern with pitch ratio 2 is found to be more effective in heat transfer enhancement than any other cases investigated.
