流刺蟹网装饵料获得增产的经验

浙江省定海县金塘区的渔民，在总路线的光辉照耀下，解放了思想，破除了迷信，树立起敢想敢说敢作的共产主义风格，在拟饵钓的尼龙布和采用白布钓蟹的启示下，在蟹流网方面也采用了上饵料的方法，首先由党支部书记包龙夫同志带头试验，结果，比不装饵的增产30％以上。现在把这种网具的规格和上饵经验分述如下：

核团微量注射下丘脑视前区“针刺灌流液”对去卵巢大鼠整体效应的研究

目的本文采用针刺一组特定穴位后的去卵巢大鼠视前区(POA)推挽灌流液,微量注射于另一个去卵巢大鼠的下丘脑POA,观察其是否可作为一种针刺信息分子的载体,产生相应的(针刺)效应。为建立一种能在离体组织和培养细胞上,研究针刺治病信号传(转)导机制的可行的实验方法。方法采用切除双侧卵巢导致下丘脑-垂体-卵巢轴(HPOA)功能异常的大鼠模型。将动物分成收集灌流液组和注射灌流液组两大组。收集灌流液组又分为收集电针后下丘脑POA推挽灌流液(简称“针刺灌流液”)组(OVXAP)和去卵巢大鼠POA灌流液组(OVXP);OVXAP给予电针“关元”为主的一组穴位处理,以阴道脱落细胞学检查和R IA测定针刺后动物血雌二醇(E2),作为判断针效的指标;用HPLC测定“针刺灌流液”中的单胺类和氨基酸递质及β-内啡肽的含量作为质控标准,对“针刺灌流液”进行质量控制;注射灌流液组又分为注射“针刺灌流液”组(IOVXAP)、注射灌流液对照组(IOVXP)和注射人工脑脊液对照组(IaCSF)。注射前后分别作动物阴道脱落细胞学检查和血E2水平测定,以观察其是否可作为一种针刺信息分子载体,将针刺效应转移到新的个体,产生针刺样效应。结果IOVXAP大鼠血E2显著高于IOVXP和IaCSF(P<0.05),且IOVXAP大鼠阴道涂片出现大量成熟脱落细胞,而IOVXP和IaCSF未出现类似的变化。结论“针刺灌流液”可能作为一种针刺信息分子的载体,把针刺信息转移到其他个体上,模拟特定的针刺效应,为进一步研究针刺治病信号的传(转)导机制提供新的实验方法。

刺吸引流-调气和血法治疗高血压性出血型脑卒中急性期临床研究

目的:观察“刺吸引流-调气和血法”对高血压性出血型脑卒中急性期的临床治疗效果。方法:选择符合病例纳入标准的高血压性出血型脑卒中患者64例,治疗组33例,在CT引导下,采用微创,穿刺脑内局部血肿并吸出引流,术后在常规内科治疗的基础上运用中药调气和血治疗;对照组31例,行内科保守治疗。将两组患者治疗后14d的神经功能缺损评分、血浆TNF-αI、L-6含量作为近期疗效指标进行比较,将治疗后3月时的日常生活能力分级作为远期疗效指标进行比较。结果:近期疗效比较,治疗组能明显减少高血压性出血型脑卒中急性期患者的神经功能缺损评分,降低血浆TNF-αI、L-6含量,与对照组比较有显著性差异(P<0.05);远期疗效比较,治疗组患者恢复良好率为72.7%,死亡率为12.1%,明显优于对照组(P<0.05)。结论:“刺吸引流-调气和血法”是高血压性出血型脑卒中急性期的一种创伤小、疗效好的中西医结合治疗方法。

关于《官井洋大黄鱼繁殖保护区管理规定修正案(草案)》的说明

(2010年11月25日在福建省第十一届人民代表大会常务委员会第十八次会议上)主任、各位副主任、秘书长、各位委员:我受省人民政府委托,现就《官井洋大黄鱼繁殖保护区管理规定修正案(草案)》作如下说明:一、修改《规定》的必要性《官井洋大黄鱼繁殖保护区管理规定》(以下简称《规定》)的颁布实施,对保护大黄鱼自然种群及其基因,发展大黄鱼人工繁殖,促进大黄鱼资源的良性循环发挥了重要作用。

Effects of spinal cord stimulation on cerebrovascular flow: role of sym-pathetic and parasympathetic innervations

Objective Cervical spinal cord stimulation （SCS） has been found to augment cerebral blood flow （CBF） in a number of animal models. However, the effective use of SCS is hampered by a lack of understanding of its mechanism（s） of action. In this paper, we focus on the sympathetic and parasympathetic effects of SCS on CBF. Method SpragueDawley rats were selected for the experimental series. The animals were divided into 5 groups to underwent SCS and laser Doppler flowmeter （LDF） recordings. Control group, the animal underwent SCS and LDF recordings without any surgery of the nerve fibers and ganglia. V 1 group, the animal underwent bilateral resection of the nasociliary and post-ganglionic parasympathetic nerve fibbers. SCG group, the animal underwent bilateral resection of supper cervical ganglion. V 1 ＋ SCG group, the animal underwent both surgeries as V1- and SCG-group animals did. Sham group, the animal underwent the carotid manipulation with blunt-tipped forceps as well as the dissection of nasociliary and post-ganglionic parasympathetic nerve fibers around the ethmoidal foramen, but without cutting any nerves. Results During the SCS, the LDF was no statistical difference between the V 1 or SCG group and the control group. Yet, the effects of SCS on CBF are completely abolished in V1＋ SCG group. Conclusions Surgical interruption of both the parasympathetic and sympathetic pathways has the contradict effect on SCS-induced CBF augmentation.

Single Polar Compound Bioelectret Material and Its Influence on the Cell Growth

To explore the influence of compound bioelectret material′s dielectric property on the cell growth,several kinds of compound bioelectret materials of collagen/chitosan were developed Their TSDC(thermally stimulated depolarization current)spectra were analyzed,and the compound bioelectret collagen/chitosan whose t α and I α were 37℃ and 2×10 -9 A respectively at polarized state was selected The cell culture study showed that the compound bioelectret material could promote normal cell growth when singly negatively polarized,and could inhibit cancer cell growth when singly positively polarized It proves that the rational designation of compound bioelectret has a broad application for clinical medicine

新型倾斜式框剌网

现在，在水库、湖泊、河流捕捞中，刺网生产的鱼货约占淡水捕捞产量的60～80％。

IMMUNOLOGICAL STUDY ON ACUPUNCTURE TREATMENT OF COLLAGEN-INDUCED ARTHRITIS IN CX43-KNOCK-OUT RATS

Objective To observe the therapeutic effect of acupuncture for collagen-induced arthritis （CIA） in Cx43 knock-out mice and its underlying immunological mechanism.Methods Heterozygote （Cx43^＋/-） mice （n=64） and wild-type （Cx43^＋/＋） mice （n=46） were used in the present study.CIA model was es- tablished by intracutaneous injection of bovine collagenⅡ（immunonization）.Acupuncture of Zúsānlǐ（足三里ST36） was carried out from the 4^th week on after the initial immunization,once a day for 3 weeks.Arthritis score and days of onset of CIA were recorded.Intracellular contents of Th subgroups of splenic lymphocytes were detected with flow cytometry.Results The incidence rate of CIA and arthritis score in Cx43^＋/- mice were significantly lower than those in Cx43^＋/＋ mice （P〈0.05）.In Cx43^＋/＋ mice,3 weeks after acupuncture treatment,the score of arthritis symptoms and signs in acupuncture group was significantly lower than that in model group （P〈0.01）,while in Cx43^＋/- mice,no significant difference was found between model group and acupuncture group in arthritis score （P〉0.05）.In comparison with control group,percentages of both Thl and Th2 and Th1/Th2 increased significantly in 0x43^＋/＋ CIA model group （P〈0.05）,while compared with model group,the percentage of Th1,Th2 and Th1/Th2 in acupuncture group decreased evidently （P〈0.05） or moderately,showing that acupuncture can effectively suppress arthritis-induced increase of Thl in Cx43^＋/＋ mice.In 0x43^＋/- mice,Th1 level and Th1/Th2 of CIA model group increased considerably in comparison with control group （P〈0.05）,while compared with model group,the Th1,Th2 and Th1/Th2 of acupuncture group had no significant changes （P〉0.05）,indicating that the effect of acupuncture in suppressing increase of Th1 percent and Th1/Th2 was eliminated.Conclusion Acupuncture of Zúsānlǐ（足三里ST 36） can effectively relieve CIA symptoms and signs and inhibit increase of splenic Th1 in Cx43^＋/＋ mice but has no any effect on CIA score,Th1,Th2 and Th1/Th2 in Cx43^＋/- mice.It shows the effect of acupuncture in improving CIA is closely related to Cx43 gene.

Preparation and evaluation of ophthalmic thermosensitive in situ gels of penciclovir

Aim To develop pluronic F127 （PF127） based formulations of penciclovir （PCV） aimed at enhancing its ocular bioavailability. Methods Thermosensitive in situ gels of penciclovir were prepared through combination of HPMC K4M or carbopol 934P and pluronic F127. Optimized formulations were examined through measuring gelation temperature, rheology speciality, drug release behavior, pharmacokinetics and ocular irritation. Results The gelation temperature was reduced by adding HPMC K4M or carbopol 934P, and the viscosity was enhanced slightly. Either HPMC K4M or carbopol 934P delayed the release of PCV from in situ gel. PCV was released by non-Fickian diffusion. The study of ocular irritation for different PCV formulations did not show any irritation or damage for the cornea. PCV bioavailability from combination of carbopol 934P and pluronic F127 gels was higher than that obtained from any other gels. Conclusion Pluronic F127 formulations of PCV can be used as liquid for administration by instilling into the eye. Facilitated by the appropriate eye temperature, the formulations were transformed to gel phase. On the basis of in vitro and in vivo results, PCV formulations containing HPMC K4M or carbopol 934P and low concentration of pluronic F127 （12%） showed potential for use as a drug delivery system with improved ocular bioavailability.

The runoff characteristics and harmonic analysis of the soil moisture dynamics in Robinia pseudoacacia stand

Robinia pseudoacacia stands act as a typical ecological protection forest in hilly semi-arid area of China. Two fields of surface runoff were separately set up in R. pseudoacacia stand and its clearcut area in the western Liaoning Province (1850-12225 E, 4024-4234 N) for measuring the characteristics of runoff and sediment as well as soil moisture dynamics. Contractive analysis of the two land types showed that there existed a significant difference in volumes of runoff and sediment between the sites of R. pseudoacacia stand and its clearcut area. The runoff volume and sediment volume in clearcut area were much bigger than those in R. pseudoacacia stand, with an increase amount of 40%-177% for runoff and 180%-400% for sediment. Hydrograph of surface runoff of typical rainfall showed that the peak value of runoff in R. pseudoacacia stand was decreased by 1.0-2.5?0-3m3s-1 compared with that in its clearcut area, and the occurring time of peak value of runoff in R. pseudoacacia stand was 10-20 min later than that in its clearcut area. Harmonic analysis of soil moisture dynamics indicated that the soil moisture in R. pseudoacacia stand was 2.3 % higher than that in clearcut area, and the soil moisture both in R. pseudoacacia stand and its clearcut area could be divided into dry season and humid season and varied periodically with annual rainfall precipitation. It was concluded that R. pseudoacacia stand plays a very important role in storing water, increasing soil moisture, and reducing surface runoff and soil erosion.

Study Under AC Stimulation on Excitement Properties of Weighted Small-World Biological Neural Networks with Side-Restrain Mechanism

In this paper, we propose a new model of weighted small-world biological neural networks based on biophysical Hodgkin-Huxley neurons with side-restrain mechanism. Then we study excitement properties of the model under alternating current （AC） stimulation. The study shows that the excitement properties in the networks are preferably consistent with the behavior properties of a brain nervous system under different AC stimuli, such as refractory period and the brain neural excitement response induced by different intensities of noise and coupling. The results of the study have reference worthiness for the brain nerve electrophysiology and epistemological science.

Effects of unsaturated fatty acids on calcium-activated potassium current in gastric myocytes of guinea pigs

AIM: To investigate the effects of exogenous unsaturated fatty acids on calcium-activated potassium current [IK(Ca)] in gastric antral circular myocytes of guinea pigs. METHODS: Gastric myocytes were isolated by collagenase from the antral circular layer of guinea pig stomach. The whole-cell patch clamp technique was used to record /K(Ca) in the isolated single smooth muscle cells with or without different concentrations of arachidonic acid (AA), linoleic acid (LA), and oleic acid (OA). RESULTS: AA at concentrations of 2,5 and 10 μmol/L markedly increased IK(Ca) in a dose-dependent manner. LA at concentrations of 5, 10 and 20 μmol/L also enhanced /K(Ca) in a dose-dependent manner. The increasing potency of AA, LA, and oleic acid (OA) on /K(Ca)at the same concentration (10μmol/L) was in the order of AA>LA>OA. AA (10 μmol/L)-induced increase of Ik(ca) was not blocked by H-7 (10 μmol/L), an inhibitor of protein kinase C (PKC), or indomethacin (10 μmol/L), an inhibitor of the cyclooxygenase pathway, and 17-octadecynoic acid (10 μmol/L), an inhibitor of the cytochrome P450 pathway, but weakened by nordihydroguaiaretic acid (10μmol/L), an inhibitor of the lipoxygenase pathway. CONCLUSION: Unsaturated fatty acids markedly increase Ik(Ca), and the enhancing potencies are related to the number of double bonds in the fatty acid chain. The lipoxygenase pathway of unsaturated fatty acid metabolism is involved in the unsaturated fatty acid-induced increase of IK(Ca) in gastric antral circular myocytes of guinea pigs.

Influence of Clothing Fabrics on Skin Microcirculation

This study investigated the effects of clothing fabric on human skin microcirculation.Once skin is covered with a clothing fabric,human sensations,namely,coolness,warmth,softness,and roughness,are aroused immediately,and the cutaneous microcirculation may be changed consequently.Since the complex relationships of the human skin,the environment,and the clothing,there is few publication focusing on the physiological responses of the skin to the fabrics.In this paper,a Laser Doppler Flowmetry(LDF)was used to test the dynamic responses of the skin blood flow when the fabric was placed on the skin.Effects of different fabrics on the skin blood flux were investigated.The results show that cold stimulation of fabric has remarkable influences on the skin blood flux,and the surface properties of fabric are of importance to affect the human skin blood flow.

Dynamic Overmodulation in the Synchronous Reference Frame for IPMSMs

Overmodulation is frequently required during the transient interval at high speed operations of IPMSMs （interior permanent magnet synchronous motors）. In this work, an overmodulation method is developed in the synchronous reference frame with a provision of assigning different priorities to d- and q-axes currents. During the current boosting in motoring, the d-axis current is increased by priority for a fast current response, and to minimize the overmodulation period. However in the regeneration process, the q-axis current is decreased by priority during a load reduction. Simulation and experimental results show that the proposed method, compared with the existing minimum-phase error method, is better for a fast current response, and in shortening the overmodulation period.

STUDY ON SCALP ELECTROACUPUNCTURE TREATMENT OF APOPLEXY AND ITS INFLUENCE ON HEMORHEOLOGY IN ISCHEMIC APOPLEXY PATIENTS

Objectives: To compare the therapeutic effect of scalp electroacupuncture with that of conventional body acupuncture for apoplexy (wind stroke) and to observe the influence of both acupuncture therapies on hemorheology of ischemic apoplexy.Methods: 183 patients, whose diseases are attributed to apoplexy according to the diagnostic criteria, are randomly divided into treatment group (93 cases) and control group (90 cases) and treated respectively with scalp electroacupuncture and conventional body acupuncture. 11 items of hemorheology of patients’ blood samples are tested with NXE-1 Viscometer which are taken in the early morning before and after treatment under fasting condition. Results: Both scalp electroacupuncture and conventional body acupuncture exert therapeutic effect for apoplexy, but the effect of scalp electroacupuncture is obviously better than that of conventional acupuncture (P< 0.05 ); both treatment group and control group are statistically significant in reducing plasma viscosity, high shear reduced viscosity, low shear reduced viscosity, erythrocyte sedimentation rate, hematocrit and fibrinogen, (P<0.01 and 0.05); while the treatment group is statistically significant in reducing whole blood viscosity, ratio of whole blood viscosity, erythrocyte index of rigidity and electrophoresis time of erythrocyte (P<0.01～ 0.05), but the control group is not significant statistically in influencing these indexes (P>0.05).

EFFECT OF ACUPUNCTURE ON BLOOD FLOW OF VERTEBRAL AND BASILAR ARTERIES IN STROKE PATIENTS

Objective: To observe the short-term effect and long-term effect of acupuncture on blood flow of vertebral and basilar arteries in stroke patients. Methods: A total of 58 stroke patients (34 males and 24 females) were agreed to participate in this study, and changes of blood flow of the vertebral and basilar arteries were detected after one week’s and 45 days’ continuous acupuncture treatment by using a Doppler’s ultrasonic diagnosis apparatus. Bilateral Fengchi (GB 20), Wangu (GB 12), Tianzhu (BL 10), etc. were punctured and stimulated with small amplitude, high frequency and twirling-reinforcing method for 3 min. The treatment was conducted once daily. Results: Following one week’s and 45 days’ acupuncture treatment, diastolic velocity (DV), systolic velocity (SV) and mean velocity (MV) of the basilar artery increased significantly in comparison with pre-treatment (P<0.05～0.01). After one week’s acupuncture treatment, DV, SV and MV of the vertebral artery on the affected side of the brain and after 45 days’ treatment, DV, SV and MV of the vertebral artery on the bilateral sides of the brain all increased considerably compared with their individual basic levels of pre-treatment (P<0.05～0.01). No significant differences were found between pre-treatment and post-treatment in pulse index (PI) of the basilar artery and bilateral vertebral arteries (P> 0.05). Results indicated that acupuncture treatment could effectively improve blood supply of the basilar artery and regulate the uneven blood flow of the bilateral vertebral arteries. Conclusion: Acupuncture treatment can raise blood flow of the brain in stroke patients.

INFLUENCE OF THE FIVE ELEMENT THEORY ON ACUPUNCTUROLOGY

In the present paper, the authors make a simple comment on the influence of the theory of five elements of TCM on the acupuncturology from ① its influence on the meridian-collateral theory and acupoint system; ② its influence on acu-moxibustion therapeutics including the application of mother and son points for reinforcing and reducing, the application of the five element theory to the midnight-noon ebb-flow needling, and guiding point selection in different seasons; and ③ its influence on acupuncture techniques.

EFFECT OF ELECTROACUPUNCTURE ON CEREBRAL BLOOD FLOW, VIP AND ET IN RATS WITH VASCULAR DEMENTIA

Objective: To investigate the relationship between electroacupuncture (EA)-induced improvement of regional cerebral blood flow and the alternations of vasoactive intestinal peptide (VIP) and endothelin (ET) in rats with experimental vascular dementia (VD). Methods: 40 Wistar rats were evenly randomized into sham-operation, model, medication (Nimotone) and EA groups. Vascular dementia model was established by repeated cerebral ischemia-reperfusion which was induced by occlusion and reopen of the bilateral common carotid arteries. EA (2～200 Hz, 2～3 mA) was applied to "Baihui"(GV 20), "Dazhui"(GV 14) and "Zusanli"(ST 36) for 30 min, once daily and continuously for 15 days. The regional cerebral blood flow (rCBF) in parietal lobe and hippocampus was determined with method of hydrogen clearance; a step-down avoidance test was adopted to observe the rats’ behavior change; and plasma VIP and ET contents were assayed by radioimmunoassay. Results: In comparison with sham-operation group, the correct rate of step-down avoidance test, rCBF in parietal lobe and hippocampus and plasma VIP level in VD model group lowered significantly (P<0.01) and plasma ET increased considerably (P<0.01). However, compared with model group, the correct rate of step-down avoidance test, rCBF values and plasma VIP in EA group raised obviously while plasma ET declined significantly. No significant differences were found between EA and medication groups in the 4 indexes. Conclusion: EA can raise rCBF in the parietal lobe and hippocampus, elevate plasma VIP level and reduce plasma ET in rats with VD.

Possible effect of fluid shear stress on osteoclastogenesis

Bone remodeling is performed under the joint action of osteoblasts and osteoclasts. Since the effect of osteoclasts has been gradually recognized on bone and joint diseases, targeted researches toward osteoclasts have become a hot research field. This article reviews the relevant medical literature concerning the possible effects of the fluid shear stress (FSS) on the osteoclastogenesis chiefly from the aspects of RANKL-RANK-OPG system, the macrophage colony-stimulating factor (M-CSF), and calcitonin receptor (CTR). On the basis of the changes of the expression of osteoclastic activities, it is suggested that FSS is a potent, important regulator of bone metabolism.

INFLUENCE OF ELECTROACUPUNCTURE ON ARTIFICIAL ABORTION- INDUCED SIDE EFFECTS

In the present study, the effect of electroacupuncture (EA) of acupoints of Ren, Spleen and Stomach Meridians on artificial abortion induced side effects was observed in 100 artificial abortion women. In comparison with 45 artificial abortion women in the control group (who had not accepted EA treatment), EA possessed significant effects in relieving abdominal pain, reducing vaginal bleeding duration, lowering infection rate and infertility rate after artificial abortion operation.