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氮源流加对Alcaligenes eutrophus积累聚β-羟基丁酸的影响 被引量:3
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作者 堵国成 陈坚 +2 位作者 尹洪波 高海军 伦世仪 《微生物学报》 CAS CSCD 北大核心 2000年第3期290-295,共6页
以真养产碱杆菌 (Alcaligeneseutrophus)为聚 β 羟基丁酸 (PHB)的生产菌株 ,在分析了PHB发酵过程参数变化的基础上 ,进一步探讨了PHB合成期不同的硫酸铵流加速率对PHB合成的影响。研究结果表明 ,在PHB合成阶段 ,培养基中氮源的完全缺... 以真养产碱杆菌 (Alcaligeneseutrophus)为聚 β 羟基丁酸 (PHB)的生产菌株 ,在分析了PHB发酵过程参数变化的基础上 ,进一步探讨了PHB合成期不同的硫酸铵流加速率对PHB合成的影响。研究结果表明 ,在PHB合成阶段 ,培养基中氮源的完全缺乏 ,导致细胞合成PHB能力的下降 ;在PHB合成期 ,不同的氮源流加速率对PHB合成过程存在着显著的影响 ,当流加速率较小时 ,尽管最终胞内PHB含量很高 ,但细胞干重、PHB浓度和PHB生产强度都较低。当氮源流加速率过大时 ,会导致最终胞内PHB含量显著下降 ,使PHB浓度和PHB生产强度降低。当硫酸铵流加速率在 0 5g/h左右时 ,可以得到较好的发酵效果。 展开更多
关键词 聚Β-羟基丁酸 发酵 硫酸铵 流加速率 合成期
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基于结晶动力学模型对胞苷酸初晶点的研究
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作者 张湜 杜远超 +2 位作者 王玲玲 袁奇 陈凯 《现代化工》 CAS CSCD 北大核心 2012年第2期85-88,共4页
结晶提纯5'-胞苷酸二钠的过程存在明显的不确定性和高度的非线性,其中初晶点无法在线检测,给过程优化策略的实施带来了阻碍。利用单纯形法估计出浓液浓度预测模型的参数,通过浓液浓度的变化预测初晶点范围,并建立了关于流加速率对... 结晶提纯5'-胞苷酸二钠的过程存在明显的不确定性和高度的非线性,其中初晶点无法在线检测,给过程优化策略的实施带来了阻碍。利用单纯形法估计出浓液浓度预测模型的参数,通过浓液浓度的变化预测初晶点范围,并建立了关于流加速率对初晶点的模型。模型结果与实验结果基本吻合,建立的模型有效。 展开更多
关键词 5’-胞苷酸二钠 结晶 初晶点 动力学模型 流加速率
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枯草杆菌Bacillus sp F26产过氧化氢酶的发酵条件 被引量:2
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作者 华兆哲 燕国梁 +1 位作者 堵国成 陈坚 《食品与生物技术学报》 CAS CSCD 北大核心 2007年第1期77-83,共7页
从内蒙古呼伦贝尔草原的盐碱湖中分离到的一株低度嗜盐嗜碱细菌Bacillus sp F26,能积累高水平过氧化氢酶(CAT)。对Bacillus sp F26发酵产过氧化氢酶的环境与营养条件的研究结果表明,其积累高水平过氧化氢酶的适宜环境条件为:温度3... 从内蒙古呼伦贝尔草原的盐碱湖中分离到的一株低度嗜盐嗜碱细菌Bacillus sp F26,能积累高水平过氧化氢酶(CAT)。对Bacillus sp F26发酵产过氧化氢酶的环境与营养条件的研究结果表明,其积累高水平过氧化氢酶的适宜环境条件为:温度37℃,种龄20-22h,接种量5%,装液量50mL/(250mL的摇瓶)。适宜发酵培养基组成(g/L)为:葡萄糖15,牛肉膏10,玉米浆10,酵母膏5,磷酸二氢钾1,氯化镁0.2,氯化钠50,碳酸钠10。采用上述条件进行摇瓶分批发酵实验,发酵20h,过氧化氢酶酶活达到16.32U/mL,细胞干重为4.12g/L。进一步研究发现,在对数生长后期(16h)添加2mmol/L的H2O2可以明显刺激产酶,在5L的发酵罐上进一步以指数速率方式流加H2O2,由于该流加方式可降低H2O2对细胞的毒害作用,过氧化氢酶酶活达到29.89U/mL,与分批发酵相比提高了92.8%。 展开更多
关键词 低度嗜盐嗜碱细菌 BACILLUS SP F26 过氧化氢酶 发酵优化 过氧化氢 指数速率
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产朊假丝酵母发酵生产谷胱甘肽的补料研究 被引量:3
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作者 尹良鸿 廖鲜艳 +3 位作者 吴晓玉 李江华 堵国成 陈坚 《工业微生物》 CAS CSCD 2009年第1期1-5,共5页
为了实现产朊假丝酵母(Candida utilis WSH02-08)的高密度培养和提高谷胱甘肽(glutathione,GSH)产量,在分批发酵研究的基础上,考察了指数速率流加和DO-stat反馈流加对产朊假丝酵母合成GSH的影响。结果表明,采用指数速率流加可获得高细... 为了实现产朊假丝酵母(Candida utilis WSH02-08)的高密度培养和提高谷胱甘肽(glutathione,GSH)产量,在分批发酵研究的基础上,考察了指数速率流加和DO-stat反馈流加对产朊假丝酵母合成GSH的影响。结果表明,采用指数速率流加可获得高细胞密度,但不利于GSH的合成,而采用DO-stat反馈流加较适宜细胞积累GSH。因此,提出并运用了指数速率-DO-stat组合流加策略,即采用指数速率流加实现细胞高密度培养,采用DO-stat反馈流加实现GSH的高积累,细胞量、GSH产量和胞内GSH含量分别为82.5 g/L、1120.6 mg/L和1.46%,实现了高细胞密度和高胞内GSH含量的相对统一。 展开更多
关键词 产朊假丝酵母 谷胱甘肽 指数速率 DO—stat反馈 组合方式
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Periodic Packing Mode for Trickle-Bed Reactors: Experiments and Modeling 被引量:2
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作者 刘国柱 米镇涛 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2004年第3期372-378,共7页
A periodic packing mode of trickle-bed reactor (TBR) for the gas limited reaction was proposed. Hy-drogenation of 2-ethylanthraquinone over Pd/Al2O3 in a laboratory-scale TBR was taken as a test reaction for determini... A periodic packing mode of trickle-bed reactor (TBR) for the gas limited reaction was proposed. Hy-drogenation of 2-ethylanthraquinone over Pd/Al2O3 in a laboratory-scale TBR was taken as a test reaction for determining whether the periodic packing mode is advantageous. The effects of operating conditions and packing type on TBR performance were experimentally examined to demonstrate the cause-effect relationships. A mathe-matic model of TBR considering axial dispersion and fractional wetting was developed to quantitatively illuminate the reason of performance enhancement. 展开更多
关键词 trickle-bed reactor periodic packing mode MODELING HYDROGENATION 2- ethylanthraquinone
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Flow behavior and fracture of Al−Mg−Si alloy at cryogenic temperatures 被引量:3
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作者 Danielle Cristina Camilo MAGALHAES Andrea Madeira KLIAUGA Vitor Luiz SORDI 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2021年第3期595-608,共14页
The tensile and fracture behaviors of AA6061 alloy were investigated in order to provide quantitative data about this alloy at cryogenic temperatures.Specimens of AA6061 alloy were solution heat treated before tensile... The tensile and fracture behaviors of AA6061 alloy were investigated in order to provide quantitative data about this alloy at cryogenic temperatures.Specimens of AA6061 alloy were solution heat treated before tensile tests at 298,173 and 77 K and tested at strain rates in the range from 0.1 to 0.0001 s^(−1).The results indicate the suppression of the Portevin−Le Chatelier(PLC)effect and dynamic strain aging(DSA)at 77 K.In contrast,at 298 K,a remarkable serrated flow,characteristic of the PLC effect,is observed.Furthermore,the tensile behavior at 77 K,compared with that observed at 173 and 298 K,shows a simultaneous increase in strength,uniform elongation,modulus of toughness,strain-hardening exponent and strain rate sensitivity,which is related to a decrease in the dynamic recovery rate at low temperature.These responses are reflected on the fracture morphology,since the dimple size decreases at 77 K,while the area covered by dimples increases.Comparisons of the Johnson−Cook model show that a good agreement can be obtained for tests at 173 and 77 K,in which DSA is suppressed. 展开更多
关键词 cryogenic temperature aluminum alloy flow behavior strain rate sensitivity work-hardening behavior Johnson−Cook model FRACTURE
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Principal Denudation Processes and Their Contribution to Fluvial Suspended Sediment Yields in the Upper Yangtze River Basin and Volga River Basin 被引量:1
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作者 VALENTIN Golosov ZHANG Xin-bao +2 位作者 HE Xiu-bin TANG Qiang ZHOU Ping 《Journal of Mountain Science》 SCIE CSCD 2015年第1期101-122,共22页
This paper synthesized the principal land denudation processes and their role in determining riverine suspended sediment yields(SSY) in two typical geographical environments of the Upper Yangtze River Basin in China a... This paper synthesized the principal land denudation processes and their role in determining riverine suspended sediment yields(SSY) in two typical geographical environments of the Upper Yangtze River Basin in China and the Volga River Basin in Eastern Europe. In the Upper Yangtze River Basin, natural factors including topography, climate,lithology and tectonic activity are responsible for the spatial variation in the magnitude of denudation rates.Human disturbances have contributed to the temporal changes of soil erosion and fluvial SSY during the past decades. On one hand, land use change caused by deforestation and land reclamation has played an important role in the acceleration of sediment production from the central hilly area and lower Jinsha catchment; On the other hand, diverse soil conservation practices(e.g., reforestation,terracing) have contributed to a reduction of soil erosion and sediment production since the late 1980 s.It was difficult to explicitly decouple the effect of mitigation measures in the Lower Jinsha River Basindue to the complexity associated with sediment redistribution within river channels(active channel migration and significant sedimentation). The whole basin can be subdivided into seven sub-regions according to the different proportional inputs of principal denudation processes to riverine SSY. In the Volga River Basin, anthropogenic sheet, rill and gully erosion are the predominant denudation processes in the southern region, while channel bank and bed erosion constitutes the main source of riverine suspended sediment flux in the northern part of the basin. Distribution of cultivated lands significantly determined the intensity of denudation processes.Local relief characteristics also considerably influence soil erosion rates and SSY in the southern Volga River Basin. Lithology, soil cover and climate conditions determined the spatial distribution of sheet, rill and gully erosion intensity, but they play a secondary role in SSY spatial variation. 展开更多
关键词 Land denudation Anthropogenicdisturbance Suspended sediment yield UpperYangtze River Volga River
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Heat Transfer and Flow Analysis in Loop Heat Pipe with Multiple Evaporators Using Network Model
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作者 Shigeki Hirasawa Tsuyoshi Kawanami Katsuaki Shirai 《Journal of Mechanics Engineering and Automation》 2016年第7期319-325,共7页
Thermal performance of a loop heat pipe with two evaporators and two condensers was examined using a lumped network model analysis. Thermosyphon-type vertical loop heat pipe and capillary-pump-type horizontal loop hea... Thermal performance of a loop heat pipe with two evaporators and two condensers was examined using a lumped network model analysis. Thermosyphon-type vertical loop heat pipe and capillary-pump-type horizontal loop heat pipe were calculated by examining the change of heating rate of two evaporators. Calculation results showed that the vapor and liquid flow rates in the loop heat pipe and the thermal conductance of the heat pipe changed significantly depending on the distribution ratio of the heating rate of the multiple evaporators. The thermal performance of the vertical loop heat pipe with two evaporators was also examined and experimental results of flow direction and thermal conductance of the heat pipe agreed with the analytical results. The lumped network model analysis is therefore considered accurate and preferable for the practical design of a loop heat pipe with multiple evaporators. 展开更多
关键词 Loop heat pipe multiple evaporators thermal conductance network model analysis two phase flow.
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Preliminary Research on Primary Energy Source of High Current Electron Beam Accelerator Based on Spiral Pulse Forming Line
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作者 詹天文 刘金亮 +1 位作者 葛斌 殷毅 《Chinese Physics C》 SCIE CAS CSCD 北大核心 2008年第z1期295-297,共3页
In this paper,the primary energy source of high current electron beam accelerator based on spiral pulse forming line is investigated.It consists of the constant-current power supply,the high voltage pulse capacitor,th... In this paper,the primary energy source of high current electron beam accelerator based on spiral pulse forming line is investigated.It consists of the constant-current power supply,the high voltage pulse capacitor,the field distortion switch,and the protection system.The primary energy source can discharge to the primary winding of the transformer with high voltage pulses whose amplitude of voltage is 40kV,current is 80kA,pulse width is 8μs and repetition frequency is less than 5Hz.The primary energy source is applied to a high current electron beam accelerator, and is featured by its compactness,stability and reliability. 展开更多
关键词 primary energy source repetition frequency high current electron beam accelerator
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吸水链霉菌发酵生产谷氨酰胺转胺酶的补料研究 被引量:1
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作者 许晓娟 张东旭 +3 位作者 王淼 李江华 陈坚 堵国成 《中国生物工程杂志》 CAS CSCD 北大核心 2009年第5期78-82,共5页
在吸水链霉菌(Streptomyces hygroscopicus)分批发酵研究的基础上,通过在菌体生长阶段指数流加葡萄糖,进行高细胞密度培养,获得了较高的菌体量;待菌体生长进入产酶期后,通过补加氮源,为产酶提供充足的氮源,其中通过流加蛋白质氮源,可以... 在吸水链霉菌(Streptomyces hygroscopicus)分批发酵研究的基础上,通过在菌体生长阶段指数流加葡萄糖,进行高细胞密度培养,获得了较高的菌体量;待菌体生长进入产酶期后,通过补加氮源,为产酶提供充足的氮源,其中通过流加蛋白质氮源,可以减少蛋白酶对成熟MTG的分解,促进产酶。结果表明,8~16h采用较高的的比生长速率(0.15h-1),后期降低比生长速率(0.10h-1),此时得到的菌体量较高,可达到36g/L,比分批发酵下的菌体量提高了80%。同时在培养基中添加50g/L的豆饼粉,最终酶活可达到5.79U/ml,提高了83%。 展开更多
关键词 吸水链霉菌 高密度培养 谷氨酰胺转胺酶 指数速率 氮源
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Assessment of Plant-Driven Mineral Weathering in an Aggrading Forested Watershed in Subtropical China 被引量:3
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作者 ZUO Shuangmiao YANG Jinling +2 位作者 HUANG Laiming David G.ROSSITER ZHANG Ganlin 《Pedosphere》 SCIE CAS CSCD 2016年第6期817-828,共12页
Plant growth contributes to mineral weathering, but this contribution remains poorly understood. Weathering rates in an aggrading forested watershed in subtropical China were studied by means of geochemical mass balan... Plant growth contributes to mineral weathering, but this contribution remains poorly understood. Weathering rates in an aggrading forested watershed in subtropical China were studied by means of geochemical mass balance. Rainfall, dry deposition, and streamwater were monitored from March 2007 to February 2012. Samples of vegetative components, rainfall, dry deposition, streamwater, representative soils, and parent rock were collected and determined for mass balance calculation and clarifying plant-driven weathering mechanisms stoichiometrically. Ignoring biomass, weathering rates of Ca^2+, Mg^2+, Na^+, and Si were 25.6, 10.7, 2.8, and 51.0 kg ha^-1 year^-1, respectively. Taking biomass into consideration, weathering rates of Ca^2+, Mg^2+, and Si and the sum of weathering rates of Ca^2+, Mg^2+, Na^+, K^+, and Si were 2.6, 1.8, 1.2, and 1.5-fold higher than those ignoring biomass, respectively. This is attributed to plant-driven weathering due to the nutrient (e.g., Ca^2+, Mg^2+, and K^+) absorption by vegetation and substantial proton production during assimilation of these nutrients, with the former acting as a pump for removing weathering products and the latter being a source of weathering agents solubilizing mineral components. The same pattern of weathering, i.e., higher rates of weathering with than without including biomass in mass balance calculation, was reported in previous studies; however, the extent to which plants drive weathering rates varied with vegetation types and climatic zones. The documented biological weathering driven by plants is expected to play a critical role in regulating nutrient cycling and material flows within the Earth's Critical Zone. 展开更多
关键词 BIOGEOCHEMISTRY BIOMASS Earth's Critical Zone geochemical mass balance nutrient absorption stoichiometric analysis vegetation weathering mechanisms
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