制备型高效液相色谱(preparative high performance liquid chromatography,Prep-HPLC)是一种使用高压、大流量液体输送系统在高分辨率、大内径、高载量分离柱上进行样品高纯度分离的液相色谱制备方法.应用该方法分离的产品在纯度... 制备型高效液相色谱(preparative high performance liquid chromatography,Prep-HPLC)是一种使用高压、大流量液体输送系统在高分辨率、大内径、高载量分离柱上进行样品高纯度分离的液相色谱制备方法.应用该方法分离的产品在纯度、回收率、分离效率等方面远远优于传统的制备方法,因此在生物制品和药物研究、生产领域得到广泛应用[1-3].本文就近年来Prep-HPLC方法的研究进展及其在生物医药制品领域的应用做一综述.……展开更多
The flow behavior in porous media with threshold pressure gradient(TPG) is more complex than Darcy flow and the equations of motion, and outer boundary and inner boundary with TPG are also different from Darcy flow fo...The flow behavior in porous media with threshold pressure gradient(TPG) is more complex than Darcy flow and the equations of motion, and outer boundary and inner boundary with TPG are also different from Darcy flow for unsteady flow of a producing well in a reservoir. An analytic method to solve this kind of problem is in a need of reestablishment. The classical method of Green's function and Newman product principle in a new way are used to solve the unsteady state flow problems of various shapes of well and reservoir while considering the TPG. Four Green's functions of point, line, band and circle while considering the TPG are achieved. Then, two well models of vertical well and horizontal well are built and simultaneously the function to calculate the moving boundary of each well model is provided. The results show that when considering TPG the pressure field is much different, which has a sudden pressure change, with a moving boundary in it. And the moving boundary of each well model increases with time but slows down rapidly, especially when the TGP is large.展开更多
Anthropogenic activities have become more and more important in characterizing the landscape, but their impacts are still restricted by natural environments. This paper discusses the interactions of anthropogenic acti...Anthropogenic activities have become more and more important in characterizing the landscape, but their impacts are still restricted by natural environments. This paper discusses the interactions of anthropogenic activity, vegetation activity and topography through describing the spatial distribution of land cover and vegetation activity (represented by Normalized Difference Vegetation Index, NDVI) along topographic gradient in a mountainous area of southwestern China. Our results indicate that the existing landscape pattern is controlled by anthropogenic activities as well as topographic factors. Intensive anthropogenic activities mainly occur in areas with relatively low elevation, gentle and concave slopes, as these areas are easy and convenient to attain for human. Because of the destruction by human, some land cover types (mainly grassland and shrub) are only found in relatively harsher environments. This study also finds that topographic wetness index (W) used in other places only reflects runoff generation capacity, but not indicate the real spatial pattern of soil water content in this area. The relationships between NDVI and W, and NDVI and length slope factor (LSF) show that runoff and erosion have complex effects on vegetation activity. Greater values of W and LSF will lead to stronger capacity to produce runoff and transport sediment, and thereby increase soil water content and soil deposition, whereas beyond a certain threshold runoff and erosion are so strong that they would destruct vegetation growth. This study provides information needed to successfully restore native vegetation, improve land management, and promote sustainable development in mountainous areas, especially for developing regions.展开更多
In this paper, a numerical study of flow in the turbulence boundary layer with adverse and pressure gradients (APGs) is conducted by using Reynolds-averaged Navier-Stokes (RANS) equations. This research chooses si...In this paper, a numerical study of flow in the turbulence boundary layer with adverse and pressure gradients (APGs) is conducted by using Reynolds-averaged Navier-Stokes (RANS) equations. This research chooses six typical turbulence models, which are critical to the computing precision, and to evaluating the issue of APGs. Local frictional resistance coefficient is compared between numerical and experimental results. The same comparisons of dimensionless averaged velocity profiles are also performed. It is found that results generated by Wilcox (2006) k-co are most close to the experimental data. Meanwhile, turbulent quantities such as turbulent kinetic energy and Reynolds-stress are also studied.展开更多
In low permeability porous media which permeability anisotropy is ubiquitous,the percolation of fluid no longer follows linear Darcy’s Law.Oil-water two phase flow equation of low permeability reservoir with permeabi...In low permeability porous media which permeability anisotropy is ubiquitous,the percolation of fluid no longer follows linear Darcy’s Law.Oil-water two phase flow equation of low permeability reservoir with permeability anisotropy is established based on generalized Darcy’s law and starting pressure gradient,corresponding finite element program is developed and simulated based on the Finite Element Program Generator system (FEPG).The results show that energy-gathering exists in the flow event of flowing area front in low permeability reservoir.In the process of energy-gathering,the flow velocity changed little but increased rapidly as soon as the pressure gradient exceeded the starting pressure gradient of the reservoir,then gradually stabilized.The greater the starting pressure gradient is,the greater the near wellbore pressure drop is,the smaller the area influenced by the reservoir pressure changes caused by water injection and oil recovery.The greater the starting pressure gradient is,the lower the water saturation in same point of the reservoir is,the smaller the water flood swept area is.There will be more difficulties in water injection to the same extent.展开更多
Based on the distribution characteristic of magnetic field along the polish wheel,the four-axis linkage technique is advanced to replace a standard five-axis one to figure low-gradient optical surfaces with a raster t...Based on the distribution characteristic of magnetic field along the polish wheel,the four-axis linkage technique is advanced to replace a standard five-axis one to figure low-gradient optical surfaces with a raster tool-path in magnetorheological finishing(MRF).After introducing the fundaments of such simplification,the figuring reachability of a four-axis system for the low-gradient optics was theoretically analyzed.Further validation including magnetic field intensity and influence function characteristic was performed to establish its application.To demonstrate the correctness,feasibility and applicability of such technique,a K4 spherical part was figured by two iterations of MRF with surface form error improved to 0.219λPV and 0.027λRMS.Meanwhile,the surface roughness was also improved a lot in MRF process.These theoretical analyses and experimental results both indicate that high form accuracy and excellent surface quality can be obtained by using the four-axis linkage technique in the process of figuring low-gradient optical elements,and the four-axis linkage system undoubtedly is much more easy to control and much more economical.展开更多
基金Project(51304220) supported by the National Natural Science Foundation of ChinaProject(3144033) supported by the Beijing Natural Science Foundation,ChinaProject(20130007120014) supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China
文摘The flow behavior in porous media with threshold pressure gradient(TPG) is more complex than Darcy flow and the equations of motion, and outer boundary and inner boundary with TPG are also different from Darcy flow for unsteady flow of a producing well in a reservoir. An analytic method to solve this kind of problem is in a need of reestablishment. The classical method of Green's function and Newman product principle in a new way are used to solve the unsteady state flow problems of various shapes of well and reservoir while considering the TPG. Four Green's functions of point, line, band and circle while considering the TPG are achieved. Then, two well models of vertical well and horizontal well are built and simultaneously the function to calculate the moving boundary of each well model is provided. The results show that when considering TPG the pressure field is much different, which has a sudden pressure change, with a moving boundary in it. And the moving boundary of each well model increases with time but slows down rapidly, especially when the TGP is large.
基金the National Natural Science Foundation of China (40621061)the Project of Chinese Academy of Sciences (KZCX2-XB2-02-31) for their financial support
文摘Anthropogenic activities have become more and more important in characterizing the landscape, but their impacts are still restricted by natural environments. This paper discusses the interactions of anthropogenic activity, vegetation activity and topography through describing the spatial distribution of land cover and vegetation activity (represented by Normalized Difference Vegetation Index, NDVI) along topographic gradient in a mountainous area of southwestern China. Our results indicate that the existing landscape pattern is controlled by anthropogenic activities as well as topographic factors. Intensive anthropogenic activities mainly occur in areas with relatively low elevation, gentle and concave slopes, as these areas are easy and convenient to attain for human. Because of the destruction by human, some land cover types (mainly grassland and shrub) are only found in relatively harsher environments. This study also finds that topographic wetness index (W) used in other places only reflects runoff generation capacity, but not indicate the real spatial pattern of soil water content in this area. The relationships between NDVI and W, and NDVI and length slope factor (LSF) show that runoff and erosion have complex effects on vegetation activity. Greater values of W and LSF will lead to stronger capacity to produce runoff and transport sediment, and thereby increase soil water content and soil deposition, whereas beyond a certain threshold runoff and erosion are so strong that they would destruct vegetation growth. This study provides information needed to successfully restore native vegetation, improve land management, and promote sustainable development in mountainous areas, especially for developing regions.
基金Foundation item: Supported by the National Natural Science Foundation of China (Nos.51309040, 51379033, 51209027, 51309025), Open Research Fund of State Key Laboratory of Ocean Engineering (Shanghai Jiao Tong University) (Grant No.1402), and Fundamental Research Fund for the Central Universities (DMU3132015089).
文摘In this paper, a numerical study of flow in the turbulence boundary layer with adverse and pressure gradients (APGs) is conducted by using Reynolds-averaged Navier-Stokes (RANS) equations. This research chooses six typical turbulence models, which are critical to the computing precision, and to evaluating the issue of APGs. Local frictional resistance coefficient is compared between numerical and experimental results. The same comparisons of dimensionless averaged velocity profiles are also performed. It is found that results generated by Wilcox (2006) k-co are most close to the experimental data. Meanwhile, turbulent quantities such as turbulent kinetic energy and Reynolds-stress are also studied.
文摘In low permeability porous media which permeability anisotropy is ubiquitous,the percolation of fluid no longer follows linear Darcy’s Law.Oil-water two phase flow equation of low permeability reservoir with permeability anisotropy is established based on generalized Darcy’s law and starting pressure gradient,corresponding finite element program is developed and simulated based on the Finite Element Program Generator system (FEPG).The results show that energy-gathering exists in the flow event of flowing area front in low permeability reservoir.In the process of energy-gathering,the flow velocity changed little but increased rapidly as soon as the pressure gradient exceeded the starting pressure gradient of the reservoir,then gradually stabilized.The greater the starting pressure gradient is,the greater the near wellbore pressure drop is,the smaller the area influenced by the reservoir pressure changes caused by water injection and oil recovery.The greater the starting pressure gradient is,the lower the water saturation in same point of the reservoir is,the smaller the water flood swept area is.There will be more difficulties in water injection to the same extent.
基金Project(91023042)supported by the National Natural Science Foundation of ChinaProject(2011CB013200)supported by the National Basic Research Program of China+1 种基金Project(B090302)supported by the Fund of Innovation,Graduate School of National University of Defense Technology,ChinaProject(CX2009B004)supported by the Hunan Provincial Innovation Foundation for Postgraduate,China
文摘Based on the distribution characteristic of magnetic field along the polish wheel,the four-axis linkage technique is advanced to replace a standard five-axis one to figure low-gradient optical surfaces with a raster tool-path in magnetorheological finishing(MRF).After introducing the fundaments of such simplification,the figuring reachability of a four-axis system for the low-gradient optics was theoretically analyzed.Further validation including magnetic field intensity and influence function characteristic was performed to establish its application.To demonstrate the correctness,feasibility and applicability of such technique,a K4 spherical part was figured by two iterations of MRF with surface form error improved to 0.219λPV and 0.027λRMS.Meanwhile,the surface roughness was also improved a lot in MRF process.These theoretical analyses and experimental results both indicate that high form accuracy and excellent surface quality can be obtained by using the four-axis linkage technique in the process of figuring low-gradient optical elements,and the four-axis linkage system undoubtedly is much more easy to control and much more economical.