To explore the applicability of anoxic-oxic (A/O) activated sludge process for petrochemical wastewater treatment,the relationship between bacterial community structure and pollutants loading/removal efficiencies was ...To explore the applicability of anoxic-oxic (A/O) activated sludge process for petrochemical wastewater treatment,the relationship between bacterial community structure and pollutants loading/removal efficiencies was investigated by gas chromatograph-mass spectrometry (GC-MS),polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and other conventional techniques.It showed that when the concentrations of the influent chemical oxygen demand (COD) and ammonia nitrogen (NH4+-N) were 420～560mg/L and 64～100mg/L,respectively,the corresponding average effluent concentra-tions were 160mg/L and 55mg/L,which were 1.6 and 2.2times higher than those of the national standards in China,respectively,demonstrating the inefficient performances of A/O process.Analysis of GC-MS indicated that refractory pollutants were mainly removed by sludge adsorption,but not by biodegradation.PCR-DGGE profile analysis suggested that the biological system was species-rich,but there was apparent succession of the bacterial community structure in different locations of the A/O system.Variations of bacterial community structure and pollutant loadings had obvious influences on pollutants removal efficiencies.Thus,A/O process was inapplicable for the treatment of complicated petrochemical wastewater,and strategies such as the reinforcement of pre-treatment and two-stage A/O process were suggested.展开更多
基金Project supported by the National Basic Research Program(973)of China(No.2004CB418505)the Science and Technology Devel-opment Program of Heilongjiang Province(No.CC05S301),China
文摘To explore the applicability of anoxic-oxic (A/O) activated sludge process for petrochemical wastewater treatment,the relationship between bacterial community structure and pollutants loading/removal efficiencies was investigated by gas chromatograph-mass spectrometry (GC-MS),polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and other conventional techniques.It showed that when the concentrations of the influent chemical oxygen demand (COD) and ammonia nitrogen (NH4+-N) were 420～560mg/L and 64～100mg/L,respectively,the corresponding average effluent concentra-tions were 160mg/L and 55mg/L,which were 1.6 and 2.2times higher than those of the national standards in China,respectively,demonstrating the inefficient performances of A/O process.Analysis of GC-MS indicated that refractory pollutants were mainly removed by sludge adsorption,but not by biodegradation.PCR-DGGE profile analysis suggested that the biological system was species-rich,but there was apparent succession of the bacterial community structure in different locations of the A/O system.Variations of bacterial community structure and pollutant loadings had obvious influences on pollutants removal efficiencies.Thus,A/O process was inapplicable for the treatment of complicated petrochemical wastewater,and strategies such as the reinforcement of pre-treatment and two-stage A/O process were suggested.
