With the progress of plant genome research, more than 50 plant metallothionein_like (MT_L) genes have been found, but only several MT_L proteins have been detected and no experimental structural information for MT_L p...With the progress of plant genome research, more than 50 plant metallothionein_like (MT_L) genes have been found, but only several MT_L proteins have been detected and no experimental structural information for MT_L proteins has been reported so far. Since detailed knowledge of the protein tertiary structure is required to understand its biological function, a method is needed to determine the structure of these proteins. In this study, the structural data of known mammal MT was used to determine the interatomic distance constraints of the CXC and CXXC motifs and the metal_sulfur chelating cluster. Then several possible MT conformations were predicted using a distance geometry algorithm. The statistical analysis was used to select those with much lower target function values and lower conformation energies as the predicted tertiary structural models of the cysteine_rich (CR) domains of these proteins. A suitable prediction method for modeling the CR domain of the plant MT_L protein was constructed. The accurately predicted result for the known structure of an MT protein from blue crab suggests that this method is practicable. The tertiary structures of CR domains of rape MT_L protein LSC54 was then modeled with this method.展开更多
With the popularity of the automatic precipitation gauges in national weather stations,testing their performance and adjusting their measurements are top priorities. Additionally,because different climatic conditions ...With the popularity of the automatic precipitation gauges in national weather stations,testing their performance and adjusting their measurements are top priorities. Additionally,because different climatic conditions may have different effects on the performance of the precipitation gauges, it is also necessary to test the gauges in different areas. This study mainly analyzed precipitation measurements from the single-Altershielded TRwS204 automatic weighing gauge(TRwS_(SA)) relative to the adjusted manual measurements(reference precipitation) from the Chinese standard precipitation gauge in a doublefence wind shield(CSPG_(DF)) in the Hulu watershed in the Qilian Mountains, China. The measurements were compared over the period from August 2014 to July2017, and the transfer function derived from the work by Kochendorfer et al.(2017 a) for correcting windinduced losses was applied to the TRwS_(SA) measurements. The results show that the average loss of TRwS_(SA) measurements relative to the reference precipitation decreased from 0.55 mm(10.7%) to 0.51 mm(9.9%) for rainfall events, from 0.35 mm(8.5%)to 0.22 mm(5.3%) for sleet events, and from 0.49 mm(18.9%) to 0.33 mm(12.7%) for snowfall events after adjustment. The uncorrected large biases of TRwS_(SA) measurements are considered to be mainly caused by specific errors of TRwS_(SA), different gauge orifice area and random errors. These types of errors must be considered when comparing precipitation measurements for different gauge types, especially in the mountains.展开更多
In this study, we measure the environmental efficiency of western China in 2000-2014 by the super-efficiency slacks-based measure model which considers the undesirable outputs. The results show that the environmental ...In this study, we measure the environmental efficiency of western China in 2000-2014 by the super-efficiency slacks-based measure model which considers the undesirable outputs. The results show that the environmental efficiency of western China is low and rank behind in the national level. The environmental efficiency is lower than the traditional efficiency which reflects that the economic growth in 2000-2014 has paid high environmental costs. The difference between the traditional efficiency and environmental efficiency experienced "inverted U"-type trend change. The environmental efficiency of the western provinces presented differentiated developing trend and the differences between the western provinces were enlarging. The environmental efficiency of the western regions was not only lower than that in the eastern regions with a big gap but also lower than that in the central regions. Through the convergence test, we found that the environmental efficiency gap between the western and the eastern regions was gradually expanding; meanwhile, the environmental efficiency gap between the central and the western regions was narrowing. This paper also analyzes the determinants of environmental efficiency by the tobit model and then addresses.展开更多
文摘With the progress of plant genome research, more than 50 plant metallothionein_like (MT_L) genes have been found, but only several MT_L proteins have been detected and no experimental structural information for MT_L proteins has been reported so far. Since detailed knowledge of the protein tertiary structure is required to understand its biological function, a method is needed to determine the structure of these proteins. In this study, the structural data of known mammal MT was used to determine the interatomic distance constraints of the CXC and CXXC motifs and the metal_sulfur chelating cluster. Then several possible MT conformations were predicted using a distance geometry algorithm. The statistical analysis was used to select those with much lower target function values and lower conformation energies as the predicted tertiary structural models of the cysteine_rich (CR) domains of these proteins. A suitable prediction method for modeling the CR domain of the plant MT_L protein was constructed. The accurately predicted result for the known structure of an MT protein from blue crab suggests that this method is practicable. The tertiary structures of CR domains of rape MT_L protein LSC54 was then modeled with this method.
基金supported primarily by the National Basic Research Program of China (2013CBA01806)the National Natural Sciences Foundation of China (41671029, 41690141, 41401040 and 41501040)
文摘With the popularity of the automatic precipitation gauges in national weather stations,testing their performance and adjusting their measurements are top priorities. Additionally,because different climatic conditions may have different effects on the performance of the precipitation gauges, it is also necessary to test the gauges in different areas. This study mainly analyzed precipitation measurements from the single-Altershielded TRwS204 automatic weighing gauge(TRwS_(SA)) relative to the adjusted manual measurements(reference precipitation) from the Chinese standard precipitation gauge in a doublefence wind shield(CSPG_(DF)) in the Hulu watershed in the Qilian Mountains, China. The measurements were compared over the period from August 2014 to July2017, and the transfer function derived from the work by Kochendorfer et al.(2017 a) for correcting windinduced losses was applied to the TRwS_(SA) measurements. The results show that the average loss of TRwS_(SA) measurements relative to the reference precipitation decreased from 0.55 mm(10.7%) to 0.51 mm(9.9%) for rainfall events, from 0.35 mm(8.5%)to 0.22 mm(5.3%) for sleet events, and from 0.49 mm(18.9%) to 0.33 mm(12.7%) for snowfall events after adjustment. The uncorrected large biases of TRwS_(SA) measurements are considered to be mainly caused by specific errors of TRwS_(SA), different gauge orifice area and random errors. These types of errors must be considered when comparing precipitation measurements for different gauge types, especially in the mountains.
基金supported by Significant Tender Project Supported by the National Social Science Foundation of China,"Study on The Crux and Key Problems of Implementing the Great Western Development Strategy in The Future Ten Years"Grant Number[10&ZD024]Key Tender Subject Supported by the National Social Science Foundation of China,"Study on Collaborative Innovation System of Urban Ecological Civilization"Grant Number[13AZD076]
文摘In this study, we measure the environmental efficiency of western China in 2000-2014 by the super-efficiency slacks-based measure model which considers the undesirable outputs. The results show that the environmental efficiency of western China is low and rank behind in the national level. The environmental efficiency is lower than the traditional efficiency which reflects that the economic growth in 2000-2014 has paid high environmental costs. The difference between the traditional efficiency and environmental efficiency experienced "inverted U"-type trend change. The environmental efficiency of the western provinces presented differentiated developing trend and the differences between the western provinces were enlarging. The environmental efficiency of the western regions was not only lower than that in the eastern regions with a big gap but also lower than that in the central regions. Through the convergence test, we found that the environmental efficiency gap between the western and the eastern regions was gradually expanding; meanwhile, the environmental efficiency gap between the central and the western regions was narrowing. This paper also analyzes the determinants of environmental efficiency by the tobit model and then addresses.