Objective.To establish a flow cytometric internal standard method for counting platelet-derived microparti-cles(PMPs)and to study its clinical significance. Methods. PMPs suspension(platelet poor plasma,PPP) was extra...Objective.To establish a flow cytometric internal standard method for counting platelet-derived microparti-cles(PMPs)and to study its clinical significance. Methods. PMPs suspension(platelet poor plasma,PPP) was extracted by gradual centrifugation. According to the size of PMPs,3 μm and 0.8μm latex beads were used as internal standards for the quantitation. PMPs were counted by adjusting flow cytometric discrimination and voltage of forward scatter and side scatter. Results. In 30 healthy donors,the average concentration of resting PMPs was(1.2×105±5.7×104 )/ml and that of activated PMPs was(1.6×106±9.1×105)/ml. Compared with healthy donors,PMPs mean value was significantly higher(P< 0.001)in 18 patients with coronary artery disease,12 with acute cerebral infraction and 23 with chronic renal failure[the average PMPs concentration,( 6.1×105±2.5×105 )/ml, ( 6.8×105±3.4×105)/ml and(5.9×105±3.1×105)/ml respectively]. However,no significant difference in PMPs concentration was observed in 25 patients with acute leukemia and severe thrombocytopenia during the aplastic phase after chemotherapy [1.3×105±6.1×104)/ml,(P >0.05)] .Conclusions. PMPs is a useful indicator in monitoring platelet activation,and plays an important role in thrombotic disease. By flow cytometric internal standard method,PMPs can be counted rapidly and accurately,which may be very helpful in interlaboratory comparative studies.展开更多
Objective: To develop a new method for the detection of TPMT gene mutations and determine the frequencies of four TPMT alleles, TPMT *1, *3A , *3B and *3C in a healthy Chinese population. Methods: A TDI-FP assay syste...Objective: To develop a new method for the detection of TPMT gene mutations and determine the frequencies of four TPMT alleles, TPMT *1, *3A , *3B and *3C in a healthy Chinese population. Methods: A TDI-FP assay system was set up in out lab. To evaluate this system, 220 healthy individuals were analyzed for the polymorphic sites at positions 460(G→A)and 719(A→G)of the TPMP gene using our new TDI–FP method. Results: Three TPMP*3C(G 460→G 719) heterozygotes were identified, TPMP *3A and TPMP *3B were not found. All mutations were confirmed by conventional DNA sequencing analysis. Conclusion: TDI-FP method has proven to be very efficient as a rapid and accurate approach for TPMP genotyping. TPMP *3C was the only polymorphism identified in this clinical samples we have registered.展开更多
Gateway floor heave control is the key to guarantee mine safe and efficient production. Through analysis of floor instability characteristics and bolting control, gateway floor strata show second level stress under ab...Gateway floor heave control is the key to guarantee mine safe and efficient production. Through analysis of floor instability characteristics and bolting control, gateway floor strata show second level stress under abutment pressure, which causes plastic flow failure in floor strata; gateway floor instability shows mainly shear-break slippage of ＂triangle sliding body＂. Mechanics of floor bolting is mainly a function of connection and combination. Main area of bolting control lies in two gateway floor angles. The paper analyzes mechanics principle of gateway floor instability, constructs stability mechanics model of gateway floor bolting, obtains gateway floor stability criterion of different bolting angles and optimum formula of bolting parameters, carries out the engineering example, and guides better field application. It provides theoretical base for bolting gateway floor instability control.展开更多
For entire roller embedded shapemeter roll, the relationship between the value of interference fit and the sensor pre-pressure, and the pressure transfer performance of shapemeter roll were analyzed by elasticity theo...For entire roller embedded shapemeter roll, the relationship between the value of interference fit and the sensor pre-pressure, and the pressure transfer performance of shapemeter roll were analyzed by elasticity theory during the cold reversible rolling process. Considering the influence of strip temperature on the interference fit, the distributions of contact pressure of the framework's top surface and the sensor pre-pressure on different values of interference fit were analyzed by the finite element technology. The results show that the contact pressure of the framework's top surface and the sensor pre-pressure increase with the increase of the value of interference fit. When the value of interference fit is between 0.05 mm and 0.09 mm, roll body's inner hole surface, the framework and pressure magnetic sensitive component don't separate from each other, and the sensor works in the linear segment of characteristic curve, so the normal operation of shapemeter roll is guaranteed.展开更多
文摘Objective.To establish a flow cytometric internal standard method for counting platelet-derived microparti-cles(PMPs)and to study its clinical significance. Methods. PMPs suspension(platelet poor plasma,PPP) was extracted by gradual centrifugation. According to the size of PMPs,3 μm and 0.8μm latex beads were used as internal standards for the quantitation. PMPs were counted by adjusting flow cytometric discrimination and voltage of forward scatter and side scatter. Results. In 30 healthy donors,the average concentration of resting PMPs was(1.2×105±5.7×104 )/ml and that of activated PMPs was(1.6×106±9.1×105)/ml. Compared with healthy donors,PMPs mean value was significantly higher(P< 0.001)in 18 patients with coronary artery disease,12 with acute cerebral infraction and 23 with chronic renal failure[the average PMPs concentration,( 6.1×105±2.5×105 )/ml, ( 6.8×105±3.4×105)/ml and(5.9×105±3.1×105)/ml respectively]. However,no significant difference in PMPs concentration was observed in 25 patients with acute leukemia and severe thrombocytopenia during the aplastic phase after chemotherapy [1.3×105±6.1×104)/ml,(P >0.05)] .Conclusions. PMPs is a useful indicator in monitoring platelet activation,and plays an important role in thrombotic disease. By flow cytometric internal standard method,PMPs can be counted rapidly and accurately,which may be very helpful in interlaboratory comparative studies.
文摘Objective: To develop a new method for the detection of TPMT gene mutations and determine the frequencies of four TPMT alleles, TPMT *1, *3A , *3B and *3C in a healthy Chinese population. Methods: A TDI-FP assay system was set up in out lab. To evaluate this system, 220 healthy individuals were analyzed for the polymorphic sites at positions 460(G→A)and 719(A→G)of the TPMP gene using our new TDI–FP method. Results: Three TPMP*3C(G 460→G 719) heterozygotes were identified, TPMP *3A and TPMP *3B were not found. All mutations were confirmed by conventional DNA sequencing analysis. Conclusion: TDI-FP method has proven to be very efficient as a rapid and accurate approach for TPMP genotyping. TPMP *3C was the only polymorphism identified in this clinical samples we have registered.
基金Supported by the National Natural Science Foundation of China （50874037）
文摘Gateway floor heave control is the key to guarantee mine safe and efficient production. Through analysis of floor instability characteristics and bolting control, gateway floor strata show second level stress under abutment pressure, which causes plastic flow failure in floor strata; gateway floor instability shows mainly shear-break slippage of ＂triangle sliding body＂. Mechanics of floor bolting is mainly a function of connection and combination. Main area of bolting control lies in two gateway floor angles. The paper analyzes mechanics principle of gateway floor instability, constructs stability mechanics model of gateway floor bolting, obtains gateway floor stability criterion of different bolting angles and optimum formula of bolting parameters, carries out the engineering example, and guides better field application. It provides theoretical base for bolting gateway floor instability control.
基金Project(2011BAF15B00)supported by the National Science and Technology Support Plan of ChinaProject(E2011203004)supported by the Hebei Provincial Natural Science Iron and Steel Joint Research Fund Program,China
文摘For entire roller embedded shapemeter roll, the relationship between the value of interference fit and the sensor pre-pressure, and the pressure transfer performance of shapemeter roll were analyzed by elasticity theory during the cold reversible rolling process. Considering the influence of strip temperature on the interference fit, the distributions of contact pressure of the framework's top surface and the sensor pre-pressure on different values of interference fit were analyzed by the finite element technology. The results show that the contact pressure of the framework's top surface and the sensor pre-pressure increase with the increase of the value of interference fit. When the value of interference fit is between 0.05 mm and 0.09 mm, roll body's inner hole surface, the framework and pressure magnetic sensitive component don't separate from each other, and the sensor works in the linear segment of characteristic curve, so the normal operation of shapemeter roll is guaranteed.
