高级建筑大修翻建时白蚁综合治理实施方案的探讨

对已建成的高级建筑发现白蚁危害严重，应采取那些防治措施，如何控制蚁害，保证房屋建筑不再遭受危害，是一个新课题。关键是根据其建筑结构、白蚁种类、危害程度、周围环境等进行勘察调研掌握第一手资料，从而有针对性的制定综合治理方案，采用先进的技术和合理的施工方法，才能达到长期而有效的白蚁防治。我们于1987年对遭受白蚁严重危害的杭州华侨饭店在大修翻建时经治理至今二十多年来未发现白蚁危害，达到预期效果。现将综合治理的实施方案提供给大家参考。

Expression Detection of DMRTs and Two sox9 Genes in Takifugu rubripes (Tetraodontidae, Vertebrata)

Sex determination and sex differentiation are important phenomena in fish, but the mechanisms of sex determination in Takifugu rubripes are poorly understood. In our study, the expression patterns of genes for DMRTs （DMRT1, DMRT2 and DMRT3）,sox9a and sox9b in T. rubripes tissues were verified with the Reverse Transcription （RT）-PCR detection. It is showed that DMRT1 expressions in testis and ovaries were much lower, and no expressions were fotmd in muscle, blood and tailfin. However, expressions for DMRT2 and DMRT3 were not found in the tissues stated above. Transcripts of sox9a were detected in muscle, fin, ovary and testis, but not in blood, whereas sox9b expression was only detected in ovary. The expression patterns of DMRTs, sox9a and sox9b in T. rubripes gonads suggest that these genes may not be sex-specific.

Small objects detection in UAV aerial images based on improved Faster R-CNN

In order to solve the problem of small objects detection in unmanned aerial vehicle(UAV)aerial images with complex background,a general detection method for multi-scale small objects based on Faster region-based convolutional neural network(Faster R-CNN)is proposed.The bird’s nest on the high-voltage tower is taken as the research object.Firstly,we use the improved convolutional neural network ResNet101 to extract object features,and then use multi-scale sliding windows to obtain the object region proposals on the convolution feature maps with different resolutions.Finally,a deconvolution operation is added to further enhance the selected feature map with higher resolution,and then it taken as a feature mapping layer of the region proposals passing to the object detection sub-network.The detection results of the bird’s nest in UAV aerial images show that the proposed method can precisely detect small objects in aerial images.

A niche model to predict Microcystis bloom decline in Chaohu Lake, China

Cyanobacterial blooms occur frequently in lakes due to eutrophication. Although a number of models have been proposed to forecast algal blooms, a good and applicable method is still lacking. This study explored a simple and effective mathematical-ecological model to evaluate the growth status and predict the population dynamics of Microcystis blooms. In this study, phytoplankton were collected and identified from 8 sampling sites in Chaohu Lake every month from July to October, 2010. The niche breadth and niche overlap of common species were calculated using standard equations, and the potential relative growth rates of Microcystis were calculated as a weighted-value of niche overlap. In July, the potential relative growth rate was 2.79 (a.u., arbitrary units) but then rapidly declined in the following months to -3.99 a.u. in September. A significant correlation (R=0.998, P<0.01) was found in the model between the net-increase in biomass of Microcystis in the field and the predicted values calculated by the niche model, we concluded that the niche model is suitable for forecasting the dynamics of Microcystis blooms. Redundancy analysis indicated that decreases in water temperature, dissolved oxygen and total dissolved phosphorus might be major factors underlying bloom decline. Based on the theory of community succession being caused by resource competition, the growth and decline of blooms can be predicted from a community structure. This may provide a basis for early warning and control of algal blooms.

Detection of Mycoplasma Hyorhinis Infection in Ovarian Cancer with in situ Hybridization and Immunohistochemistry

OBJECTIVE To detect Mycoplasma hyorhinis in ovarian cancer tissues and the relationship between mycoplasma infection and ovarian cancer. METHODS All specimens obtained from 109 cases with ovarian cancer were fixed in freshly prepared 10% neutral buffered formalin, embedded in paraffin, and cut into 4-μm sections for insitu hybridization （ISH） and then detected with immunohistochemistry （IHC）. The expressions of 16S rRNA and P37 protein from mycoplasma hyorhinis were detected respectively using ISH and IHC. SPSS 13.0 software was employed to analyze the relationship between the results of the study and clinical pathological materials. RESULTS The expression rate of mycoplasma hyorhinis 16S rRNA gene and P37 protein was 20.2% （22/109） and 43.1% （47/109 cases） in ovarian cancer tissues, respectively, but it was 0 （0/30 cases） in the normal ovarian tissues. The difference in mycoplasma infection ratio between ovarian cancer tissues and normal tissues was extremely significant （P 〈 0.001）. Anyhow, we didn＇t found any association between the mycoplasma infection and clinical pathological characters. CONCLUSION There was a mycoplasma infection in ovarian cancer tissues, which may play a role in oncogenesis of ovarian cancer.

Determination of Epibionts of the Marine Turtle Lepidochelys Ofivacea （Eschscholtz, 1829） Nesting in Ceuta Beach, Sinaloa, Mexico

Epibionts are floristic and faunistic benthonic organisms living attached to the skin of another animal; this phenomenon is well known as epibiosis and has been documented for all species of marine turtles. The interaction between the turtle and epibiotic organisms is to provide substrate for the epibionts communities, their presence are based mainly in the shell and in other anatomical structures in lower intensity. It has been registered that some epibionts affect growth, and can cause diverse damage in anatomical structures like the eyes, mouth, and limbs. They are usually vectors of disease. For such reasons the objective of the present work is to determinate the epibionts species associated with the marine turtle Lepidochelys olivacea in Ceuta beach, Sinaloa, Mexico. During the nesting season May-December of 2008-2009, the female turtles nesting and strandings were monitored and epibionts were collected. Eleven species distributed in the following taxa were obtained： fishes of the family Echeneidae, Hirudineos （Ozobranchus branchiatus, Ozobranchus margoi）, Arthropods （Chelonibia testudinaria, Lepas sp., Conchoderma virgatum） and the first registry of Stephanolepas muricata for Lepidochelys olivacea. According to the records from Ceuta Beach the species of epibionts are still being registered with the exception of Ozobranchus margoi, and Stephanolepas muricata, thus, suggesting further research on epibiont organisms.

Expression of the chemoresistance-related proteins and p53 gene as a predictor of chemotherapy response and a prognostic factor in patients with ovarian cancer at the stages of II-IV

Objective To clarify the prognostic significance of histologic subtype and its correlation to expression of chemoresistance-related proteins （CRPs） in ovarian cancer. Methods A total of 107 stage II-IV ovarian cancers, where the proportion of serous, endometrioid, mucinous, and clear cell subtype was 62.6%, 15.9%, 14.0%, and 7.5%, respectively, were investigated for glutathione Stransferase-pi （GST-pi）, MDR （multidrug resistance）-l, and p53 expression using immunohistochemistry. Results GST-pi expression was detected in 62.6% of the tumors and was not related to histologic subtype of tumor. MDR-1 expression was observed in 52.3% of the tumors tested and was more frequently detected in serous adenocarcinomas than other histologic subtypes of tumor （P〈0.001）. P53 expression was found in 43.3% of serous, 35.3% of endometrioid, 40.0% of mucinous adenocarcinomas and 37.5% of clear cell adenocarcinomas. In univariate analysis, there are direct correlations between CRPs and overall survival. In multivariate analysis, GST-pi expression （P=0.0052）, MDR-1 expression （P=0.0058）, histologic subtype （P=0.0067）, FIGO stage （P=0.0089）, and residual tumor （P=0.0041） were found to be significant independent prognostic factors. Conclusion Histologic subtype proved to be the significant independent prognostic factor in addition to FlGO stage and residual tumor in stage II-IV ovarian cancer. GST-pi, MDR-1, and p53 expression pattern is closely related to histologic subtype of ovarian cancer, at the same time they are the significant predictors of survival.

Detection of ＂ Candidatus Phytoplasma asteris＂ in Brussels Sprout and Its Possible Association with Flower Bud Failure in Poland

Severe growth abnormalities including shoot stunting, leaf blade reduction and flower bud failure of Brussels sprout were observed in Poland. The presence of phytoplasma in diseased as well as in healthy looking plants, was demonstrated by nested polymerase chain reaction assay employing phytoplasma universal rRNA primer pairs-P1/P7 followed by R16F2n/R16R2. Products of PCR primed by R 16F2n/R 16R2 primer pair from naturally infected Brussels sprouts were sequenced. Comparison of the obtained 16S rDNAs revealed high nucleotide sequence identity between analyzed phytoplasma isolates （99.8%-100%）. They were also nearly identical with the sequences of other phytoplasmas isolates from sub-group 16SrI-B, and they were classified as members of ＂Candidatus Phytoplasma asteris＂.