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增加ABI-377自动测序仪测序长度的改进措施
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作者 刘敬忠 李文艳 Steve S.Sommer 《中华检验医学杂志》 CAS CSCD 北大核心 2002年第1期41-41,T001,共2页
关键词 DNA技术 ABI-377自动 测序长度
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血管紧张素转换酶基因多态性与白族脑血管病患者的相关性研究 被引量:2
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作者 许虹 范茜君 +4 位作者 郭春 聂荔 常履华 聂志 杨桂益 《中风与神经疾病杂志》 CAS CSCD 北大核心 2010年第3期211-214,共4页
目的研究血管紧张素转换酶(ACE)基因插入/缺失多态性与云南大理白族脑血管病患者的相关性。方法应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术,对73例白族脑血管病(ICVD)组患者(其中脑梗死40例,脑出血33例)和43例性别年龄相... 目的研究血管紧张素转换酶(ACE)基因插入/缺失多态性与云南大理白族脑血管病患者的相关性。方法应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术,对73例白族脑血管病(ICVD)组患者(其中脑梗死40例,脑出血33例)和43例性别年龄相匹配的白族健康对照组进行ACE基因型检测和基因插入/缺失多态性分析;并进行ACE基因双向测序。结果(1)脑梗死组的DD基因型频率和D等位基因频率明显高于对照组(P<0.05);(2)脑出血组与对照组相比较,未发现DD基因型频率和D等位基因频率有明显差异(P>0.05);(3)脑血管组内伴高血压与不伴高血压,伴糖尿病与不伴糖尿病,伴血脂异常与血脂正常者相比较,未发现DD基因型频率和D等位基因频率有显著差异(P>0.05);(4)D等位基因和I等位基因其核苷酸序列和长度与国内外文献报道无明显差别。结论(1)ACE基因插入/缺失多态性与脑梗死的发生有关联性,DD基因型和D等位基因是脑梗死患者的高危因素;(2)ACE基因插入/缺失多态性与脑出血发生未发现有关联性;(3)脑血管病的其他相关危险因素如高血压,糖尿病,血脂与ACE基因多态性可能无关联;(4)ACE基因16内含子中一段287bp的插入片段造成I/D多态性。 展开更多
关键词 血管紧张素转换酶 基因多态性 脑血管病 聚合酶链反应-限制性片段长度多态性(PCR-RFLP)基因
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Sequential Detection of Digital Watermarking
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作者 李莉 俞玉莲 王沛 《Journal of Donghua University(English Edition)》 EI CAS 2005年第5期129-134,共6页
The paper analyzed a new watermarking detection paradigm including double detection thresholds based on sequential hypothesis testing. A joint design of watermarking encoding and detection was proposed. The paradigm h... The paper analyzed a new watermarking detection paradigm including double detection thresholds based on sequential hypothesis testing. A joint design of watermarking encoding and detection was proposed. The paradigm had good immunity to noisy signal attacks and high detection probability. Many experiments proved that the above algorithm can detect watermarks about 66% faster than popular detectors, which could have significant impact on many applications such as video watermarking detection and watermark-searching in a large database of digital contents. 展开更多
关键词 digital watermarking watermarking detection sequential testing watermarking length
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Investigation on the interaction length and access resistance of a nanopore with an atomic force microscopy
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作者 SI Wei YANG HaoJie +7 位作者 LI Kun WU GenSheng ZHANG Yin KAN YaJing XIE Xiao SHA JingJie LIU Lei CHEN YunFei 《Science China(Technological Sciences)》 SCIE EI CAS CSCD 2017年第4期552-560,共9页
Nanopore devices have attracted a lot of attention for their potential application in DNA sequencing. Here, we study how an occluding object placed near a nanopore affects its access resistance by integrating an atomi... Nanopore devices have attracted a lot of attention for their potential application in DNA sequencing. Here, we study how an occluding object placed near a nanopore affects its access resistance by integrating an atomic force microscopy with a nanopore sensor. It is found that there exists a critical hemisphere around the nanopore, inside which the tip of an atomic force microscopy will affect the ionic current. The radius of this hemisphere, which is a bit smaller than the theoretical capture radius of ions, increases linearly with the applied bias voltage and quadratically with the nanopore diameter, but is independent of the operation modes and scanning speeds of the atomic force microscopy. A theoretical model is also proposed to describe how the tip position and geometrical parameters affect the access resistance. 展开更多
关键词 solid-state nanopore ionic current access resistance interaction length AFM capture radius
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