A sandwich format immunochromatographic assay for detecting foot-and-mouth disease virus (FMDV) serotypes was developed. In this rapid test,affinity purified polyclonal antibodies from Guinea pigs which were immunized...A sandwich format immunochromatographic assay for detecting foot-and-mouth disease virus (FMDV) serotypes was developed. In this rapid test,affinity purified polyclonal antibodies from Guinea pigs which were immunized with sucking-mouse adapted FMD virus (A/AV88(L) strain) were conjugated to colloidal gold beads and used as the capture antibody,and affinity purified polyclonal antibodies from rabbits which were immunized with cell-culture adapted FMD virus (A/CHA/09 strain) were used as detector antibody. On the nitrocellulose membrane of the immunochromatographic strip,the capture antibody was laid on a sample pad,the detector antibody was printed at the test line(T) and goat anti-guinea pigs IgG antibodies were immobilized to the control line(C). The lower detection limit of the test for a FMDV 146S antigen is 11.7ng/ml as determined in serial tests after the strip device was assembled and the assay condition optimization. No cross reactions were found with FMDV serotype C,Swine vesicular disease (SVD),Vesicular stomatiti svirus (VSV) and vesicular exanthema of swine virus (VES) viral antigens with this rapid test. Clinically,the diagnostic sensitivity of this test for FMDV serotypes A was 88.7% which is as same as an indirect-sandwich ELISA. The specificity of this strip test was 98.2% and is comparable to the 98.7% obtained with indirect-sandwich ELISA. This rapid strip test is simple,easy and fast for clinical testing on field sites; no special instruments and skills are required,and the result can be obtained within 15 min. To our knowledge,this is the first rapid immunochromatogarpic assay for serotype A of FMDV.展开更多
The amount of microbeads ingested by Daphnia magna decreases on exposure to toxic materials; tins oenawor was used to develop a toxicity test. To determine the toxicity of seven metals, D. magna were collected and hom...The amount of microbeads ingested by Daphnia magna decreases on exposure to toxic materials; tins oenawor was used to develop a toxicity test. To determine the toxicity of seven metals, D. magna were collected and homogenized, and the fluorescence intensity of the microbeads ingested by D. magna was measured. The amount of ingestion was determined from fluorescence intensity. The fluorescence intensity was half of that of the controls which was measured as the 30 min-FI50, and these data correlated well with those from an acute immobilization method （24 h-EC50）. An advantage of the method using fluorescent beads is that an estimate of the 24 h-EC50 can be obtained.展开更多
Potency is one of the most important indexes of inactivated vaccines.A number of methods have been established to assay the potency,of which the NIH test and single-dose mouse protection test are the "prescribed ...Potency is one of the most important indexes of inactivated vaccines.A number of methods have been established to assay the potency,of which the NIH test and single-dose mouse protection test are the "prescribed methods".Here,we report a method to semi-quantitatively assay the potency of an inactivated rabies vaccine,which uses fewer animals and takes less time to complete.Depending on the quality requirements of a vaccine(e.g.minimum potency),a rabies reference vaccine is,for example,diluted to the minimum potency,and 50 μL of the dilution is taken to inoculate 10 mice.The same amount of the test rabies vaccine is inoculated into another 10 mice.After two weeks,all mice are bled and serum samples are assayed for viral neutralizing antibody by the fluorescent antibody virus neutralization(FAVN) test.By comparing the median and interquartile range of antibody titers of the reference vaccine with those of the test vaccine,the test vaccine potency can be semi-quantitatively judged as to whether it is in accord with the required quality.The reliability of this method was also confirmed in dogs.The procedure can be recommended for batch potency testing during inactivated rabies vaccine production.展开更多
This paper demonstrates a new framework of vocabulary learning processes（VLPs） with fill/ justification of involved learning steps, in response to inefficient self-directed vocabulary learning（SDVL） of college stu...This paper demonstrates a new framework of vocabulary learning processes（VLPs） with fill/ justification of involved learning steps, in response to inefficient self-directed vocabulary learning（SDVL） of college students in China's Mainland. Based on the review of established frameworks for second language acquisition and vocabulary learning, a more systematic and comprehensive framework of VLPs is constructed with six new VLPs explored and specified sequentially in a cycle. It aims to help initiate learners＇ mental efforts in a rational way and thereby achieve learners＇ long-term word retention and good word transfer. Besides, the newly-designed VLPs are embodied in authentic learning material for learners＇ self-study of vocabulary in particular domains. The efficacy of the material is also tested in an empirical study for the purpose of validating the effectiveness of these VLPs.展开更多
基金Financial supported by the Gansu ProvincialSci. & Tech. Department (1002NKDA037)
文摘A sandwich format immunochromatographic assay for detecting foot-and-mouth disease virus (FMDV) serotypes was developed. In this rapid test,affinity purified polyclonal antibodies from Guinea pigs which were immunized with sucking-mouse adapted FMD virus (A/AV88(L) strain) were conjugated to colloidal gold beads and used as the capture antibody,and affinity purified polyclonal antibodies from rabbits which were immunized with cell-culture adapted FMD virus (A/CHA/09 strain) were used as detector antibody. On the nitrocellulose membrane of the immunochromatographic strip,the capture antibody was laid on a sample pad,the detector antibody was printed at the test line(T) and goat anti-guinea pigs IgG antibodies were immobilized to the control line(C). The lower detection limit of the test for a FMDV 146S antigen is 11.7ng/ml as determined in serial tests after the strip device was assembled and the assay condition optimization. No cross reactions were found with FMDV serotype C,Swine vesicular disease (SVD),Vesicular stomatiti svirus (VSV) and vesicular exanthema of swine virus (VES) viral antigens with this rapid test. Clinically,the diagnostic sensitivity of this test for FMDV serotypes A was 88.7% which is as same as an indirect-sandwich ELISA. The specificity of this strip test was 98.2% and is comparable to the 98.7% obtained with indirect-sandwich ELISA. This rapid strip test is simple,easy and fast for clinical testing on field sites; no special instruments and skills are required,and the result can be obtained within 15 min. To our knowledge,this is the first rapid immunochromatogarpic assay for serotype A of FMDV.
文摘The amount of microbeads ingested by Daphnia magna decreases on exposure to toxic materials; tins oenawor was used to develop a toxicity test. To determine the toxicity of seven metals, D. magna were collected and homogenized, and the fluorescence intensity of the microbeads ingested by D. magna was measured. The amount of ingestion was determined from fluorescence intensity. The fluorescence intensity was half of that of the controls which was measured as the 30 min-FI50, and these data correlated well with those from an acute immobilization method （24 h-EC50）. An advantage of the method using fluorescent beads is that an estimate of the 24 h-EC50 can be obtained.
基金the China National"863"Program(Approval No.2011AA10A212)Special Fund for Agro-Scientific Research in the Public Interest(ApprovalNo.201203056)
文摘Potency is one of the most important indexes of inactivated vaccines.A number of methods have been established to assay the potency,of which the NIH test and single-dose mouse protection test are the "prescribed methods".Here,we report a method to semi-quantitatively assay the potency of an inactivated rabies vaccine,which uses fewer animals and takes less time to complete.Depending on the quality requirements of a vaccine(e.g.minimum potency),a rabies reference vaccine is,for example,diluted to the minimum potency,and 50 μL of the dilution is taken to inoculate 10 mice.The same amount of the test rabies vaccine is inoculated into another 10 mice.After two weeks,all mice are bled and serum samples are assayed for viral neutralizing antibody by the fluorescent antibody virus neutralization(FAVN) test.By comparing the median and interquartile range of antibody titers of the reference vaccine with those of the test vaccine,the test vaccine potency can be semi-quantitatively judged as to whether it is in accord with the required quality.The reliability of this method was also confirmed in dogs.The procedure can be recommended for batch potency testing during inactivated rabies vaccine production.
文摘This paper demonstrates a new framework of vocabulary learning processes（VLPs） with fill/ justification of involved learning steps, in response to inefficient self-directed vocabulary learning（SDVL） of college students in China's Mainland. Based on the review of established frameworks for second language acquisition and vocabulary learning, a more systematic and comprehensive framework of VLPs is constructed with six new VLPs explored and specified sequentially in a cycle. It aims to help initiate learners＇ mental efforts in a rational way and thereby achieve learners＇ long-term word retention and good word transfer. Besides, the newly-designed VLPs are embodied in authentic learning material for learners＇ self-study of vocabulary in particular domains. The efficacy of the material is also tested in an empirical study for the purpose of validating the effectiveness of these VLPs.
