捏指可测病疗疾

捏指(趾)可以测病疗疾的原理来自针灸经络学说，其核心是十二经脉的存在。体表的某些“点”能产生一种古人称之为“得气”而今人称之为“感觉”的传导现象，并且以有规律的线性途径呈现。五指的指尖与脚趾上各有许多经穴，他们分别与内脏有密切的关系。将指甲根的部位捏住然后用力压捻，从小手指开始，一个一个进行。如果哪个手指被捻捏时感到特别疼痛，那么就提示与此相关的内脏有病了。手指的疼痛与其对应的脏腑疾病有下列关系：

基于改进YOLOv8的轻量化小麦病害检测方法

为提高小麦病害检测精度,实现将模型方便快速部署到移动端,该研究提出了一种基于改进YOLOv8的轻量化小麦病害检测方法。首先,使用PP-LCNet模型替换YOLOv8网络结构的骨干网络,并在骨干网络层引入深度可分离卷积(depthwise separable convolution, DepthSepConv)结构,减少模型参数量,提升模型检测性能;其次,在颈部网络部分添加全局注意力机制(global attention mechanism, GAM)模块,强化特征中语义信息和位置信息,提高模型特征融合能力;然后,引入轻量级通用上采样内容感知重组(content-aware reassembly of features,CARAFE)模块,提高模型对重要特征的提取能力;最后,使用Wise-IoU(weighted interpolation of sequential evidence for intersection over union)边界损失函数代替原损失函数,提升网络边界框回归性能和对小目标病害的检测效果。试验结果表明,对于大田环境下所采集的小麦病害数据集,改进后模型的参数量及模型大小相比原YOLOv8n基线模型分别降低了12.5%和11.3%,同时精确度(precision)及平均精度均值(mean average precision,m AP)相较于原模型分别提高了4.5和1.9个百分点,优于其他对比目标检测算法,可为小麦病害检测无人机等移动端检测装备的部署和应用提供参考。

餐后感觉能自我测病

许多疾病与饮食紧密相关。因此，进食后自己的感觉不容忽视。一些异常感觉往往预示着某些疾病的到来或存在。疾病早发现、早治疗是至关重要的。1．进食油腻性食物以后，如果感到右上腹胀痛，并放射到肩部者，很可能患有胆囊炎或胆结石症。

加强鱼病测报 促进渔业发展

万州区地处三峡库区腹心，83km长江江段自西向东纵贯全境，全区有渔业水面16．8万亩，其中捕捞水面4．5万亩，可养殖水面12．3万亩，包括三峡库区可养殖水面5万亩。全区有天然鱼类近80种，其中名特经济鱼类30余种。养殖种类达26个。近几年来，万州渔业发展态势良好，2004年，全区完成水产品产量1．2万吨，其中名优水产品产量3300吨，实现渔业产值1．3亿元。渔业科研成果显著，取得岩原鲤、

脉象的解剖映射、血流映射与脉诊客观化研究的误区分析

脉诊在古今中医学里都是学术热点和临床难点，笔者提出藏象内环境自稳态学说，并以此为基础，进一步深入地发现脉诊的若干问题。

Advance of the Research on Testing of Animal Viruses by LAMP Technology in Abroad

Loop-mediated isothermal amplification（LAMP） is an amplification method developed by Notomi et al and has been applied successfully for the detection of many viruses.This paper introduced the current status of LAMP and recent developments,and the method applying in the diagnosis of animal viruses in abroad.

基于图像模式识别的纸张纸病捡测系统

根据纸张质量分级方法,设计了一种基于图形模式识别的纸张纸病捡测系统。在介绍系统的测量原理和图形模式识别系统的组成及原理的基础上,分别对CCD获取图像、基色选取、图像二值化、一次滤波、图像分析、二次滤波进行了介绍,并简单的列举CCD器件的选取、系统的构成、软件的设计、系统的技术性能指标和影响测试精度的因素。

Development of an One-step Reverse Transcription Loop-mediated Isothermal Amplification Method for Rapid Detection of Bovine Viral Diarrhea Virus

Objective] This study aimed to develop a reverse transcription loop-medi-ated isothermal amplification （RT-LAMP） method for detecting BVDV. [Method] Since gp48 gene of BVDV is among the most conserved regions, a set of four primers was designed to amplify six target sequences at the gp48 gene region for the RT-LAMP assay. The optimization of the RT-LAMP reaction was performed by evaluat-ing reaction temperature and reaction time. [Result] The RT-LAMP aasay was suc-cessful y conducted at 56 ℃ within 40 min under isothermal conditions, and the re-sults could be detected as ladder-like bands using agarose gel electrophoresis. The RT-LAMP assay is highly sensitive and able to detect 3.74 ×100 copies/μl of BVDV RNA, as no cross-reaction was observed with other viruses. [Conclusion] Overal , the newly established RT-LAMP assay indicates the potential application in both clinical diagnosis and field surveil ance of BVDV.

Short-term Prediction of Occurrence Period of Main Diseases and Pests of Flos lonicerae using Phenoecology

The occurrence periods of Semiaphis heraclei Takahashi,Frankliniella sp.,Haptonchus luteolus and Microsphara linicerae Enchson wint.in Rabenh.causing damage on Flos lonicerae were investigated in F.lonicerae planting area in XinCheng county of Guangxi Province during 2008-2010,which were coincided with the occurrence periods of related phenology of local Prunus persica Rootstock.With P.persica Rootstock as indicator plant,the occurrence periods of three species of pests and one species of disease were predicted,respectively,and the method was simple and accurate,which could be the foundation for preventing these pests and diseases in the local field.

河南省城市社区卫生服务利用现状

目的了解河南省城市社区慢性病监测网络中高血压、糖尿病患者对社区卫生服务的利用情况。方法对河南省城市社区慢性病监测试点的高血压、糖尿病患者进行面对面问卷调查。结果1/3以上的高血压、糖尿病管理患者将社区作为知识获取的第一途径;对社区卫生服务基本满意率为92.9%;6个月内到社区就诊率平均为64.6%;86.0%的高血压患者和54.1%糖尿病患者能做到1月内定期测量血压和检测血糖;60%的管理患者选择社区就诊;80.03%以上的管理患者接受了药物性治疗,有56.7%～82.3%的管理患者接受了非药物性治疗服务。结论要积极推广社区高血压、糖尿病综合防控适宜技术,加强患者规范管理,提升社区卫生服务能力,促进居民对社区卫生服务的利用。

The Research Status and Enlightenment of Foreign Foodborne Disease Prevention and Control

Foodborne disease is a worldwide il ness and over 1 bil ion people get diseased every year. In China, thousands of foodborne diseases occur every year. It is proved that prevention is the most crucial for control ing foodborne disease. In the research, food safety monitoring system, laws and early warning system in the US and EU were introduced to explore the successful experience and monitoring or control mode suitable for China.

Integrated Surveillance Method of Mosquitoes and Mosquito-borne Diseases

Monitoring on vector-borne virus lays foundation for control of vector-borne disease, and a scientific and highly-efficient surveil ance method is of great signifi-cance for prevention and control ing of vector-borne diseases. The research sum-marized mosquito and mosquito-borne disease monitoring methods and proposed problems in the monitoring system, as wel as introducing new monitoring methods at home and abroad, providing references for improvements of integrated surveil-lance of mosquito or mosquito-borne viruses.

A Rapid and Simple Method for the Detection of Rice Dwarf Virus by Aqueous Extract

Rice dwarf disease caused by rice dwarf virus （RDV） is one of the major rice virus diseases in China,which widely distributes in the rice area of China.A simple and rapid method for detection of RDV in rice plants and Nephotettix cincticeps was investigated in this study,and the whole detection only lasted for 20 min.After diseased leaves of rice （10 mg） and leaves of Nephotettix cincticeps （10 mg） both infected by RDV were ground by sterile water （100 μl）,the supernatant was analyzed by 1% agarose gel electrophoresis to steadily detect five specific electrophoretic bands with 1 000 bp to 5 000 bp.However,there was no electrophoretic band in the healthy rice leaves and aqueous extract of non-viruliferous N.cincticeps.Therefore,this method was more rapid and simple to detect RDV in rice plants and Nephotettix cincticeps,which avoided expensive reagents and tedious steps of conventional serological testing and molecular detection （RT-PCR and Western-blot）.

Regional epidemic laws of poplar Ice Nucleation Active bacterial canker

Through the methods of correlation analysis and main factor analysis, the relationship between the poplar INA bacte-rial canker and circumstances was analyzed and 9 main factors for affecting the disease were selected. Based on the compre-hensive analysis of main factors and induced factors, the standard for risk grades of this disease was promoted and northeast region of China was divided into 4 districts with different risk grades: seriously occurring district, commonly occurring district, occasionally occurring district, and un-occurring district. Nonlinear regression analysis for six model curves showed that the Richard growth model was suitable for describing the temporal dynamics of poplar INA bacterial canker. By stepwise variable selection method, the multi-variable linear regression forecasting equation was set up to predict the next year抯 disease index, and the GM (1,1) model was also set up by grey method to submit middle or long period forecast.

Genetic Variation Analysis of 3D Gene and Molecular Detection of Porcine Kobuvirus in 2013-2014

[Objective] This study aimed to investigate the prevalence and variation of porcine kobuvirus （PKV） in suckling piglets in China. [Method] In 2013-2014, 224 feces samples from suckling piglets with diarrhea in 27 pig farms of five provinces in China were collected to detect 3D genes of PKV with RT-PCR method; the sequences and genetic variation of 29 PKV 3D genes were analyzed. [Result] Total positive rate of PKV in feces samples from suckling piglets with diarrhea was 65.18% （146/224）; total positive rate of PKV in pig farms was 85,2% （23/27）; nucleotide sequences and the deduced amino acid sequences of 29 PKV 3D genes shared 87.0%-100% and 92.7%-100% homologies with six PKV-related 3D sequences, respectively. [Conclusion] PKV infection is prevalent in suckling piglets in China; PKV 3D genes exhibit high diversity.

Development of TaqMan-based Real-time PCR Assay for Detecting Transmissible Gastroenteritis Virus and Its Application in Vaccine Evaluation

[Objective] This study aimed to establish a TaqMan-based real-time PCR assay for detecting transmissible gastroenteritis virus （TGEV）. [Method] Primers and a probe were designed according to the conserved sequence of N gene in TGEV genome. After gradient dilution, the recombinant plasmid harboring the N gene was used as a standard for real-time PCR assay to establish the standard curve. [Re- sult] The results showed that the established real-time PCR assay exhibited a good linear relationship within the range of 102-10^10 copies/ul; the correlation coefficient was above 0.99 and the amplification efficiency ranged from 90% to 110%. The de- tection limit of real-time PCR assay for TGEV was 10 copies/μl, suggesting a high sensitivity; there was no cross reaction with other porcine viruses, indicating a good specificity; coefficients of variation within and among batches were lower than 3%, suggesting a good repeatability. The established real-time PCR method could be ap- plied in quantitative analysis and evaluation of the immune efficacy of TGEV vac- cines and detection of TGEV in clinical samples. [Conclusion] The TaqMan-based real-time PCR assay established in this study is highly sensitive and specific, which can provide technical means for the epidemiological survey of TGEV, development of TGEV vaccines and investigation of the pathogenesis of TGE.

Identification of Strawberry Anthracnose Pathogens and Screening of Germicides

[Objective] This study aimed to identify strawberry anthracnose pathogens and screen appropriate germicides. [Method] Strawberry anthracnose pathogens in Jurong City of Jiangsu Province were isolated and identified. The toxicities of sever-al germicides to Col etotrichum fragariae were determined with indoor mycelial growth inhibition assay. Field control test was preformed to further verify the control efficacy of germicides. [Result] The isolated strawberry anthracnose pathogenic strains were identified as C. gloeosporioide from genus Col etotrichum of subphylum Deuteromycotina. Prochloraz, pyrazole Kresoxim and hexaconazole al exhibited good indoor control effects; 25% pyrazole Kresoxim EC2000-fold solution, 24% azoxys-trobin＆#183;hexaconazole （azoxystrobin∶hexaconazole=1∶2） SC1500-fold solution and 25%prochloraz EC1000-fold solution al exhibited significant field control efficacy. [Con-clusion] The study provides reference for the alternate use of germicides in actual production.

Two cameras detect more lesions in the small-bowel than one

AIM:To explore the feasibility of dual camera capsule (DCC)small-bowel(SB)imaging and to examine if two cameras complement each other to detect more SB lesions.METHODS:Forty-one eligible,consecutive patients underwent DCC SB imaging.Two experienced investigators examined the videos and compared the total number of detected lesions to the number of lesions detected by each camera separately.Examination tolerability was assessed using a questionnaire.RESULTS:One patient was excluded.DCC cameras detected 68 positive findings(POS)in 20(50%)cases.Fifty of them were detected by the"yellow"camera,48 by the"green"and 28 by both cameras;44%(n=22)of the"yellow"camera’s POS were not detected by the"green"camera and 42%(n=20)of the"green" camera’s POS were not detected by the"yellow"camera.In two cases,only one camera detected significant findings.All participants had 216 findings of unknown significance(FUS).The"yellow","green"and both cameras detected 171,161,and 116 FUS,respectively;32%(n=55)of the"yellow"camera’s FUS were not detected by the"green"camera and 28%(n=45)of the"green"camera’s FUS were not detected by the "yellow"camera.There were no complications related to the examination,and 97.6%of the patients would repeat the examination,if necessary.CONCLUSION:DCC SB examination is feasible and well tolerated.The two cameras complement each other to detect more SB lesions.