科研经费之“测试化验加工费”在高校内部的使用与管理实践——以西南石油大学为例

随着国家对科研经费投入的不断提升,作为科研重地的高校,经费增长更为突出。科研经费的增长要求提高相应的经费管理水平。长期以来,对于地方高校,对"测试化验加工费"管理使用成为科研经费管理中的薄弱环节。如何加强管理,才能使经费使用合理、合法、合规,经广泛调研、咨询、探索,出台了"校内测试分析费管理实施细则",解决了"测试化验加工费"的使用难题,使学校纵向科研项目及科研经费得到进一步提升的同时,科研基地的人才培养功能显现、对外开放及其承担科研任务的实力和水平大幅度提升。

抓牢检测环节 打好成果基础 湖北省第三次土壤普查试点内业测试经验分享

作为第三次全国土壤普查(以下简称“土壤三普”)中承上启下的关键环节,做好内业测试化验工作是后期成果编制的基础,总结好试点工作中内业环节的经验做法,能为土壤普查工作的全面铺开打好基础。本文总结了湖北省第三次土壤普查试点工作中内业测试化验环节的经验做法,以期在全省范围内开展好土壤普查工作趟路子、摸经验。

抓进度 控质量 齐河县高质高效推进第三次全国土壤普查

齐河县按照“统一领导、部门协作、分级负责、各方参与”的要求,强化组织领导,压实工作责任,科学组织,压茬推进,严控第三次全国土壤普查各环节质量关口,确保普查质量和成效。2024年1月8日已完成土壤普查955个表层样点和10个剖面样点的内业检测及平台数据上传工作,以德州市第一的速度完成内业化验工作,为下一步有序推进土壤普查数据成果整理汇总集成工作打下了坚实基础。

关于做好第三次全国土壤普查内业质控的几点思考

四川省作为2022年第三次全国土壤普查省、市、县三级联动省份之一,七个试点县圆满完成了土壤三普试点工作。笔者根据在四川省三普办一年的内业测试化验质控试点工作经验,总结了七点实用经验做法,如采取集中制备和在线质控;将检测实验室内部质控前置;统一方法验证要求,提高实验室检测能力等,以期为三普工作的全面铺开提供借鉴。

The response of collision speed caused by the large bus to new flexible barrier

In order to study the response of collision speed caused by the large bus to new flexible barrier,in this paper,with the large bus as the carrier,the full-scale impact tests between flexible barrier and vehicle with the impact velocities of 40 km/h and 60 km/h were carried out separately,following the procedures of the test preparation,test processing,data acquisition, etc,which were based on the test platform of the Large Structure Crash Testing Laboratory of Changsha University of Science and Technology. The important test results which contain the damage of vehicles and barrier,the moving locus of vehicle,the occupant risk index,the maximum dynamic deformation, etc,were obtained through the analysis under the different collision speeds. These provide the necessary reference basis for the further research on the structure topology optimization and improve the comprehensive constraint performance to the flexible barrier.

Effects of trace element and purification on properties of AZ80 magnesium allo

The effects of trace element Fe on the corrosion behavior of AZ80 magnesium alloy were investigated by salt spray test and electrochemical measurements.The results show that the corrosion rate decreases with decreasing the trace element Fe content in an approximately linear relation even though the amount of trace element Fe reduces to 0.000 2%(mass fraction).The electrochemical measurements show that the corrosion potential(φcorr)of the alloy with lower trace element Fe content shifts to less negative value.It is suggested that the control trace element by purification is an effective way to enhance the corrosion resistance of AZ80 magnesium alloy.

Degradation behavior of aggregate skeleton in stone matrix asphalt mixture

The degradation behavior of aggregate skeleton in stone matrix asphalt mixture was investigated based on theoretical analysis, laboratory test and field materials evaluation. A stress-transfer model was established to provide the fundamental understanding of the stress distribution and degradation mechanism of stone matrix asphalt (SMA) aggregate skeleton. Based on the theoretical analysis, crushing test and superpave gyratory compactor (SGC) test were used to evaluate the degradation behavior of aggregate skeleton of SMA. To verify the laboratory test results, gradation analysis was also conducted for the field materials extracted from SMA pavements after long-time service. The results indicate that the degradation of SMA aggregate skeleton is not random but has fixed internal trend and mechanism. Special rule is found for the graded fine aggregates generated from coarse aggregate breakdown and the variation of 4.75 mm aggregate is found to play a key role in the graded aggregates to form well-balanced skeleton to bear external loading. The variation of 4.75 mm aggregate together with the breakdown ratio of aggregate gradation can be used to characterize the degradation behavior of aggregate skeleton. The crushing test and SGC test are proved to be promising in estimating the degradation behavior of SMA skeleton.

Numerical simulation on turbulent flow field in convergentdiveroent nozzle

Because of the complication of turbulence＇s mechanism and law as well as the jet pressure in nozzle is difficult to test by experiment, five turbulent models were applied to numerically simulate the turbulent flow field in convergent-divergent nozzle. Theory analysis and experiment results of mass flow rates conclude that the RNG k-ε model is the most suitable model. The pressure distribution in the convergent-divergent nozzle was revealed by computational fluid dynamic （CFD） simulating on the turbulent flow field under different pressure conditions. The growing conditions of cavitation bubbles were shown; meanwhile, the phenomena in the experiment could be explained. The differential pres- sure between the upstream and downstream in nozzle throat section can improve the cavitating effect of cavitation water jet.

Prediction method of restoring force based on online AdaBoost regression tree algorithm in hybrid test

In order to solve the poor generalization ability of the back-propagation(BP)neural network in the model updating hybrid test,a novel method called the AdaBoost regression tree algorithm is introduced into the model updating procedure in hybrid tests.During the learning phase,the regression tree is selected as a weak regression model to be trained,and then multiple trained weak regression models are integrated into a strong regression model.Finally,the training results are generated through voting by all the selected regression models.A 2-DOF nonlinear structure was numerically simulated by utilizing the online AdaBoost regression tree algorithm and the BP neural network algorithm as a contrast.The results show that the prediction accuracy of the online AdaBoost regression algorithm is 48.3%higher than that of the BP neural network algorithm,which verifies that the online AdaBoost regression tree algorithm has better generalization ability compared to the BP neural network algorithm.Furthermore,it can effectively eliminate the influence of weight initialization and improve the prediction accuracy of the restoring force in hybrid tests.

Experimental study on performance that carbon dioxide inhibits coal oxidation and spontaneous combustion

Adopting oil-bath temperature programming experiment and gas chromatography, CO2＇s inhibitory performance on spontaneous combustion of Tingnan Coal Mine sample was analyzed. Through temperature rise rate test experiment, the accuracy, stability and reliability of the improved oil-bath temperature programming system applied in this experiment was proved to be superior to the traditional system. Spontaneous combustion characters parameters test of coal sample in pure air was carried out with this system and offered comparison standard for research in next stage. Temperature programming to coal sample was further conducted in oil-bath with different concentration of CO2. Testing results are compared with parameters of concentration of CO, O2, temperature, CO generation rate and O2 consumption rate tested and calculated in previous experiment in pure air. Methods of proportioning between concentration of CO and O2, CO concentration and temperature, CO generation rate and O2 consumption rate were applied to eliminate obstructions from certain external factors such as inlet of CO2; meanwhile influences of CO2 of different concentrations to coal oxidation and spontaneous combustion were investigated. Also CO2 inhibition technique was used in spontaneous combustion prevention in workface No. 106 of Tingnan Coal Mine, data collected from which indicate that CO2 performs well in inhibiting coal oxidation and spontaneous combustion.

Development and Lubricating Properties of Rolling Oil for Stainless Steel Cold Rolling

Appropriate base oils and homologous additives such as extreme pressure and anti-wear agents,oiliness agents and antioxidants were selected,and experiments testing the compatibility performance between additive,base oil and other components were carried out to develop the SK and SD series of rolling oils for cold rolling of stainless steel.The developed oils were used in the stainless steel cold rolling lubrication experiments,and were successfully applied in the actual cold rolling operation of stainless steel.Compared with a foreign product,the tribological properties,the thermal oxidation stability,and the rolling lubrication performance of the developed stainless steel cold rolling oils were studied.Test results showed that the tribological properties of the thereby developed rolling oils and the reference one were almost at the same level,and to some extent the performance of rolling was even better than the foreign product,at the same time the stainless steel sheet could retain its well annealed performance.Meanwhile,within a certain range,the lubrication of the rolling oil became better as its viscosity increased at the same level of saponification value,which could provide a lower friction coefficient,so that a higher maximum reduction ratio of the rolled piece through a constant roll gap and a minimum thickness could be secured.Also,similar phenomena appeared as the saponification value increased at a same viscosity level of the rolling oils.

Experiment study of optimization on prediction index gases of coal spontaneous combustion

The coal of Anyuan Mine has the characteristic of easy spontaneous combustion. Conventional method is difficult to predict it. Coal samples from this mine were tested in laboratory. The data obtained from laboratory determination were initialized for the value which was defined as "K". The ratio of each index gas and value of "K", and the ratio of combination index gases and value of "K", were analyzed simultaneously. The research results show that for this coal mine, if there is carbon monoxide in the gas sample, the phenomenon of oxidation and temperature rising for coal exists in this mine; if there is C_2H_4 in the gas sample, the temperature of coal perhaps exceeds 130 °C. If the coal temperature is between 35 °C and 130 °C, prediction and forecast for coal spontaneous combustion depend on the value of Φ(CO)/K mainly; if the temperature of coal is between 130 °C and 300 °C, prediction and forecast for coal spontaneous combustion depend on the value of Φ(C_2H_6)/Φ(C_2H_2) and Φ(C_2H_6)/K. The research results provide experimental basis for the prediction of coal spontaneous combustion in Anyuan coal mine, and have better guidance on safe production of this coal mine.

The diagnosis of hyperkalemia induced by potassium chloride in experimental rabbits

Objective： To observe the electrocardiogram （ECG） and heart rate in a rabbit model ofhyperkalemia induced by potassium chloride, and provide theoretical and experimental reference for timely diagnosis of hyperkalemia clinically. Methods： Forty rabbits were randomly assigned into four groups. 0.9% isotonic saline solution was perfused in the group I , while 1.0%, 4.0% and 10.0% potassium chloride solutions were perfused in the other groups, respectively, to induce hyperkalemia. Results： The changes of serum potassium, ECG and heart rate before perfusion were significantly （P〈 0.05） different from those after perfusion in the other groups compared with group I . The effect of ECG variability rates was well consistent （Kappa = 0.724, P〈0.01） with that of serum potassium on diagnostic tests in the other groups. The area under ROC curve in diagnosis effect of ECG variability rates and sermn potassium on hyperkalaemia was much significantly larger （P〈0.01 and P〈0.05） compared with 0.5. There was asignificantly positive correlation between ECG variability rates and serum potassium （r= 0.865, P〈0.05 for bilateral Pearson test）. There was certain positive correlation between heart rate and serum potassium, but the correlation was poor （r= 0.526, R2=0.277）. Conclusion： A combination of serum potassium, ECG variability rates and heart rate should be considered and analyzed by synthesis to establish an accurate and timely diagnosis for hyperkalemia.

Physiological and Phytosanitary Potentials of Coriander and Radish Seeds

Information on seed vigor of the vegetable crops is always important and necessary due to the increasing demand of high quality seeds for sowing and high-value commercial. The cultivation of these species, conducted intensively, should be established with seed high potential physiological and health for the development of a more productive and sustainable agriculture. The present study was conducted to evaluate the potential physiological of seed lots of radish and coriander. The experiment was conducted at the laboratory of seed analysis and greenhouse of the plant science department of the Federal University of Pelotas in South Brazil. Four radish seed lots, cultivar ＂Saxa＂, and four coriander seed lots, cultivar ＂Verdito＂ were used. Germination seed test, first count of germination, accelerated aging test, electrical conductivity and seedling emergence were used to evaluate the physiological quality of the lots. The experimental design was completely randomized design with five replications. Means were compared by Tukey test. The accelerated aging test was the most efficient test in assessing the physiological quality for both lots of radish seeds and coriander and this test provide coherent results with seedlings emergence.

New double yield surface model for coarse granular materials incorporating nonlinear unified failure criterion

A new double-yield-sarface （DYS） model was developed to characterize the strength and deformation behaviors of coarse granular materials （CGMs）. Two kinds of deformation mechanisms, including the shear and compressive plastic deformation, were taken into account in this model, These two deformation mechanisms were described by the shear and compressive yield functions, respectively. The Lode angle dependent formulations of proposed model were deduced by incorporating a 3D nonlinear unified failure criterion. Some comparisons were presented between the numerical predictions of proposed model and test data of true triaxial tests on the modeled rockfills. The model predictions are in good agreement with the test data and capture the strain hardening and plastic volumetric dilation of CGMs. These findings verify the reasonability of current DYS model, and indicate that this model is well suited to reproduce the stress-strain-volume change behavior of CGMs in general.

The Establishment of Immunochemistry Test Based on a Synthetic Peptide Antibody for the Detection of a Porcine Circovirus-Like Virus P1

Recently, a novel porcine circovirus-like virus P1 with a circular DNA genome of 0.648 kb was identified. P1 antigen was detected both in vitro and in vivo by synthetic peptide-derived polyclonal antibody-based immunochemistry. The designed peptides were synthesized by solid-phase technique, purified by high performance liquid chromatography, coupled to Keyhole limpet hemocyanin, and injected into rabbits to prepare polyclonal antibody. The emergence of positive cells revealed that synthetic peptide could elicit antibodies against P1 and viral protein could be synthesized. The polyclonal peptide antibodies described here was successfully applied to immunochemical staining and proved helpful in diagnosing P1.

Use of Chromium Oxide and Alkane Indicator Methods for Determination of Feed Intake for Grazing Sheep

This experiment was carried out to determine feed intake and digestibility of grazing sheep on pasture. A total of 14 animals randomly divided into two groups with seven animals each group were used in the experiment. Digestibility of pastures was determined using two types of markers-alkane （C32-C36） and chromium oxide. Dry matter intake （DMI） was 717.22 g/d based on chromium oxide method in the experiment, while according to alkane method, DMI was 965.93 g/d and 1,051.07 g/d for C32 and C36, respectively. In conclusion, pastures met 74%-81% of crude protein （CP） and 57%-61% of energy requirements of lambs grazing on Yuzuncu Yil University pasture, who are mid-quality and 4-7 month-old with a 275 g/d expected daily gain. It was calculated that when 628-693 g/d of barley is given, both CP and metabolizable energy （ME） requirements of animals can be met.

Synthesis and Study of Glutamine Acid Containing Citrates

Terms of synthesis were defined for the creation of new generation chelate fertilizers and for their experimental testing. Glutamine acid and citric acid containing chelate citrates of the general formulae M.gI.HL＇nHO were synthesized, where, M = Mn, Zn, Fe, Co, Cu; n = 0; 1; 1.5; gl-glutamine acid; HL-citric acid anion. Identity and composition of synthesized compounds were determined by microelemental analysis, melting temperature and X-ray diffraction analysis. X-ray diffraction analysis of the compounds and glutamine acid and citric acid （H4L） was used to determine their crystalline structure and roentgen-amorphous and iso-structural orders. Their solubility was studied in various solvents.

Application of Genetic Algorithms to Optimize Neural Networks for Selected Tribological Tests

This paper presents a method of determining the friction coefficient in metal forming using multilayer artificial neural networks based on experimental data obtained from strip drawing test. The number of input variables of the artificial neural network has been optimized using genetic algorithm. This process is based on surface parameters of the sheet and dies, sheet material parameters and clamping force as input parameters to train the neural network. In addition to demonstrating the fact that regression statistics model using genetic selection and intelligent problem solver are better than models without preprocessing of input data, the sensitivity analysis of the input variables has been conducted. This avoids the time-consuming testing of neurons in finding the best network architecture. The obtained results from this study have also pointed out that genetic algorithm can successfully be applied to optimize the training set and the outputs agree with experimental results. This allows reduction or elimination of expensive experimental tests to determine friction coefficient value.

Preparation of monoclonal antibody to P53 and its clinical application

Objective：The aim of this study was to prepare monoclonal antibody against P53, a kind of tumor suppressor protein,and use the antibody initial y in clinical immunoassay. Methods：Monoclonal antibody was prepared and identified via the classic protocol of monoclonal antibody preparation. Identified monoclonal antibodies were purified by af inity chro-matography. Antibody titer was determined by enzyme linked immunosorbent assay （ELISA）. The specific binding activity of antibody was detected by Western blotting and immunohistochemistry. Results：Three strains of monoclonal antibodies named 1P15, 2P37 and 3P40 were obtained and purified by af inity chromatography. The purity of antibodies was higher than 90%. The titers of antibodies were more than 1：6000. Western blot and immunohistochemistry assay showed that the specific antibody can combine with endogenous P53 protein in the tumor celllines and determine the expression of P53 in tumor tis-sue. Conclusion：Three strains of monoclonal antibodies with high af inity to P53 were successful y established, which can be used for detecting the expression of P53 in tumor cells or tissue.