With the progress of plant genome research, more than 50 plant metallothionein_like (MT_L) genes have been found, but only several MT_L proteins have been detected and no experimental structural information for MT_L p...With the progress of plant genome research, more than 50 plant metallothionein_like (MT_L) genes have been found, but only several MT_L proteins have been detected and no experimental structural information for MT_L proteins has been reported so far. Since detailed knowledge of the protein tertiary structure is required to understand its biological function, a method is needed to determine the structure of these proteins. In this study, the structural data of known mammal MT was used to determine the interatomic distance constraints of the CXC and CXXC motifs and the metal_sulfur chelating cluster. Then several possible MT conformations were predicted using a distance geometry algorithm. The statistical analysis was used to select those with much lower target function values and lower conformation energies as the predicted tertiary structural models of the cysteine_rich (CR) domains of these proteins. A suitable prediction method for modeling the CR domain of the plant MT_L protein was constructed. The accurately predicted result for the known structure of an MT protein from blue crab suggests that this method is practicable. The tertiary structures of CR domains of rape MT_L protein LSC54 was then modeled with this method.展开更多
The existence of thermocline changes the acoustic structure and effects the direction of the stared rays. This paper analyzes the working processs of the active sorer, and the mathematical models. The detection probai...The existence of thermocline changes the acoustic structure and effects the direction of the stared rays. This paper analyzes the working processs of the active sorer, and the mathematical models. The detection probaility of the active sonar under thenmoline is studied. First, the detection distance without thermocline is estimat- ed, then the effect of thermocline＇s depth and sound velocity changes on detecting submarine probability are discussed, and based on this, the effects of the sea condition on searching submarine probability is discussed, lastly the distance of active sonar is calculatod under thermocline. The results indicate that tufter thennocline, the distance of the active sonar becomes obvious short, and with the sea condition becoming rough, the effect is more dear.展开更多
In this paper, we first combine tetra-peptide structural words with contact number for protein secondary structure prediction. We used the method of increment of diversity combined with quadratic discriminant analysis...In this paper, we first combine tetra-peptide structural words with contact number for protein secondary structure prediction. We used the method of increment of diversity combined with quadratic discriminant analysis to predict the structure of central residue for a sequence fragment. The method is used tetra-peptide structural words and long- range contact number as information resources. The accuracy of Q3 is over 83% in 194 proteins. The accuracies of predicted secondary structures for 20 amino acid residues are ranged from 81% to 88%. Moreover, we have introduced the residue long-range contact, which directly indicates the separation of contacting residue in terms of the position in the sequence, and examined the negative influence of long-range residue interactions on predicting secondary structure in a protein. The method is also compared with existing prediction methods. The results show that our method is more effective in protein secondary structures prediction.展开更多
文摘With the progress of plant genome research, more than 50 plant metallothionein_like (MT_L) genes have been found, but only several MT_L proteins have been detected and no experimental structural information for MT_L proteins has been reported so far. Since detailed knowledge of the protein tertiary structure is required to understand its biological function, a method is needed to determine the structure of these proteins. In this study, the structural data of known mammal MT was used to determine the interatomic distance constraints of the CXC and CXXC motifs and the metal_sulfur chelating cluster. Then several possible MT conformations were predicted using a distance geometry algorithm. The statistical analysis was used to select those with much lower target function values and lower conformation energies as the predicted tertiary structural models of the cysteine_rich (CR) domains of these proteins. A suitable prediction method for modeling the CR domain of the plant MT_L protein was constructed. The accurately predicted result for the known structure of an MT protein from blue crab suggests that this method is practicable. The tertiary structures of CR domains of rape MT_L protein LSC54 was then modeled with this method.
基金supported by the National Natural Science Foundation of China(No.50979009)Doctoral Fund of Ministry of Education of China(No.200801510002)the Major State Basic Research Development Program of China(973Program)(No.2009CB320805)
文摘The existence of thermocline changes the acoustic structure and effects the direction of the stared rays. This paper analyzes the working processs of the active sorer, and the mathematical models. The detection probaility of the active sonar under thenmoline is studied. First, the detection distance without thermocline is estimat- ed, then the effect of thermocline＇s depth and sound velocity changes on detecting submarine probability are discussed, and based on this, the effects of the sea condition on searching submarine probability is discussed, lastly the distance of active sonar is calculatod under thermocline. The results indicate that tufter thennocline, the distance of the active sonar becomes obvious short, and with the sea condition becoming rough, the effect is more dear.
文摘In this paper, we first combine tetra-peptide structural words with contact number for protein secondary structure prediction. We used the method of increment of diversity combined with quadratic discriminant analysis to predict the structure of central residue for a sequence fragment. The method is used tetra-peptide structural words and long- range contact number as information resources. The accuracy of Q3 is over 83% in 194 proteins. The accuracies of predicted secondary structures for 20 amino acid residues are ranged from 81% to 88%. Moreover, we have introduced the residue long-range contact, which directly indicates the separation of contacting residue in terms of the position in the sequence, and examined the negative influence of long-range residue interactions on predicting secondary structure in a protein. The method is also compared with existing prediction methods. The results show that our method is more effective in protein secondary structures prediction.
