annular beam tri-heterodyne confocal microscope has been proposed to improve the anti-environmental interference capability and the resolution of a eonfoeal microscope. It simultaneously detects far-, on-, and near-fo...annular beam tri-heterodyne confocal microscope has been proposed to improve the anti-environmental interference capability and the resolution of a eonfoeal microscope. It simultaneously detects far-, on-, and near-focus signals with given phase differences by dividing the measured light path of the eonfoeal microscope into three sub-paths （signals）. Pair-wise real-time heterodyne subtraction of the three signals is used to improve the anti-environmental interference capability, axial resolution, and linearity; and a shaped annular beam super-resolution technique is used to improve lateral resolution. Theoretical analyses and preliminary experiments indicate that an axial resolution of about 1 nm can be achieved with a shaped annular beam tri-heterodyne confoeal microscope and its lateral resolution can be better than 0.2 um for A = 632.8 nm, the numerical aperture of the lens of the microscope is NA = 0.85, and the normalized radius e = 0.5.展开更多
基金Project supported by the National Natural Science Foundation of China （Grant No 50475035）, the Doctoral Program of Higher Education of China （Grant No 20050213035） and the Program for New Century Excellent Talents in University of China （Grant No NCET-05-0348）.
文摘annular beam tri-heterodyne confocal microscope has been proposed to improve the anti-environmental interference capability and the resolution of a eonfoeal microscope. It simultaneously detects far-, on-, and near-focus signals with given phase differences by dividing the measured light path of the eonfoeal microscope into three sub-paths （signals）. Pair-wise real-time heterodyne subtraction of the three signals is used to improve the anti-environmental interference capability, axial resolution, and linearity; and a shaped annular beam super-resolution technique is used to improve lateral resolution. Theoretical analyses and preliminary experiments indicate that an axial resolution of about 1 nm can be achieved with a shaped annular beam tri-heterodyne confoeal microscope and its lateral resolution can be better than 0.2 um for A = 632.8 nm, the numerical aperture of the lens of the microscope is NA = 0.85, and the normalized radius e = 0.5.
