The distributed passive measurement is an important technology for networkbehavior research. To achieve a consistent measurement, the same packets should be sampled atdistributed measurement points. And in order to es...The distributed passive measurement is an important technology for networkbehavior research. To achieve a consistent measurement, the same packets should be sampled atdistributed measurement points. And in order to estimate the character of traffic statistics, thetraffic sample should be random in statistics. A distributed samplingmask measurement model isintroduced to tackle the difficulty of measuring the full trace of high-speed networks. The keypoint of the model is to choose some bits that are suitable to be sampling mask. In the paper, thebit entropy and bit flow entropy of IP packet headers in CERNET backbone are analyzed, and we findthat the 16 bits of identification field in IP packet header are fit to the matching field ofsampling mask. Measurement traffic also can be used to analyze the statistical character ofmeasurement sample and the randomicity of the model. At the same time the experiment resultsindicate that the model has a good sampling performance.展开更多
[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []V...[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients （FF） of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.展开更多
The self-absorption of γ-ray emitted from cylindrical fissile materials, such as 235 U and 239 Pu, does not possess spherical symmetry. The analytical formulae of self-absorption for γ-ray throughout the cylinder ha...The self-absorption of γ-ray emitted from cylindrical fissile materials, such as 235 U and 239 Pu, does not possess spherical symmetry. The analytical formulae of self-absorption for γ-ray throughout the cylinder have been obtained. The intensity of γ-ray is a function of γ-ray outgoing directions and cylindrical configurations, accordingly one can acquire the information about geometrical configuration of cylindrical fissile materials through multi-location measurements. Further more, the method is given in this article. The result can be applied to the fissile material safe- guard, such as nuclear monitoring and verifying.展开更多
文摘The distributed passive measurement is an important technology for networkbehavior research. To achieve a consistent measurement, the same packets should be sampled atdistributed measurement points. And in order to estimate the character of traffic statistics, thetraffic sample should be random in statistics. A distributed samplingmask measurement model isintroduced to tackle the difficulty of measuring the full trace of high-speed networks. The keypoint of the model is to choose some bits that are suitable to be sampling mask. In the paper, thebit entropy and bit flow entropy of IP packet headers in CERNET backbone are analyzed, and we findthat the 16 bits of identification field in IP packet header are fit to the matching field ofsampling mask. Measurement traffic also can be used to analyze the statistical character ofmeasurement sample and the randomicity of the model. At the same time the experiment resultsindicate that the model has a good sampling performance.
基金Supported by National Natural Science Foundation of China(31072155)Natural Science Foundation of Jiangsu Province(BK2010068)+1 种基金Fund for Independent Innovation of Agricultural Science in Jiangsu Province[CX(11)2060]Special Fund for Agroscientific Research in the Public Interest(201303041)~~
文摘[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients （FF） of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.
文摘The self-absorption of γ-ray emitted from cylindrical fissile materials, such as 235 U and 239 Pu, does not possess spherical symmetry. The analytical formulae of self-absorption for γ-ray throughout the cylinder have been obtained. The intensity of γ-ray is a function of γ-ray outgoing directions and cylindrical configurations, accordingly one can acquire the information about geometrical configuration of cylindrical fissile materials through multi-location measurements. Further more, the method is given in this article. The result can be applied to the fissile material safe- guard, such as nuclear monitoring and verifying.
