通过优化测定土壤8种有机氯农药净化方法的最优条件,分析对比浓硫酸净化法和层析柱净化法的净化效果。结果表明:浓硫酸净化的最佳条件为:10 mL 50 g/L的硫酸钠溶液洗涤冲洗2~3次,用铜粉脱硫,回收率为:83.9%~113.5%;层析柱净化的最佳条件...通过优化测定土壤8种有机氯农药净化方法的最优条件,分析对比浓硫酸净化法和层析柱净化法的净化效果。结果表明:浓硫酸净化的最佳条件为:10 mL 50 g/L的硫酸钠溶液洗涤冲洗2~3次,用铜粉脱硫,回收率为:83.9%~113.5%;层析柱净化的最佳条件为:以丙酮-正己烷1∶9混合溶剂为洗脱剂,洗脱剂用量为40 mL,回收率为:96.5%~108.2%;两种净化方法均有比较的净化效果,浓硫酸净化能大部分消除基体干扰,呈现更好的效果。展开更多
Axenic microalgal strains are highly valued in diverse microalgal studies and applications. Antibiotics, alone or in combination, are often used to avoid bacterial contamination during microalgal isolation and culture...Axenic microalgal strains are highly valued in diverse microalgal studies and applications. Antibiotics, alone or in combination, are often used to avoid bacterial contamination during microalgal isolation and culture. In our preliminary trials, we found that many microalgae ceased growing in antibiotics at extremely high concentrations but could resume growth quickly when returned to an antibiotics-free liquid medium and formed colonies when spread on a solid medium. We developed a simple and highly efficient method of obtaining axenic microalgal cultures based on this observation. First, microalgal strains of different species or strains were treated with a mixture of ampicillin, gentamycin sulfate, kanamycin, neomycin and streptomycin （each at a concentration of 600 mg/L） for 3 days; they were then transferred to antibiotics-free medium for 5 days; and finally they were spread on solid f/2 media to allow algal colonies to form. With this method, five strains ofNannochloropsis sp. （Eustigmatophyceae）, two strains of Cylindrotheca sp. （Bacillariophyceae）, two strains of Tetraselmis sp. （Chlorodendrophyceae） and one strain ofAmphikrikos sp. （Trebouxiophyceae） were purified successfully. The method shows promise for batch- purifying microalgal cultures.展开更多
文摘通过优化测定土壤8种有机氯农药净化方法的最优条件,分析对比浓硫酸净化法和层析柱净化法的净化效果。结果表明:浓硫酸净化的最佳条件为:10 mL 50 g/L的硫酸钠溶液洗涤冲洗2~3次,用铜粉脱硫,回收率为:83.9%~113.5%;层析柱净化的最佳条件为:以丙酮-正己烷1∶9混合溶剂为洗脱剂,洗脱剂用量为40 mL,回收率为:96.5%~108.2%;两种净化方法均有比较的净化效果,浓硫酸净化能大部分消除基体干扰,呈现更好的效果。
基金Supported by the National Basic Research Program of China(973 Program)(No.2011CB200901)the National Technical Supporting Project Foundation(No.2011BAD14B01)the National High Technology Research and Development Program of China(863 Program)(No.2013AA065801)
文摘Axenic microalgal strains are highly valued in diverse microalgal studies and applications. Antibiotics, alone or in combination, are often used to avoid bacterial contamination during microalgal isolation and culture. In our preliminary trials, we found that many microalgae ceased growing in antibiotics at extremely high concentrations but could resume growth quickly when returned to an antibiotics-free liquid medium and formed colonies when spread on a solid medium. We developed a simple and highly efficient method of obtaining axenic microalgal cultures based on this observation. First, microalgal strains of different species or strains were treated with a mixture of ampicillin, gentamycin sulfate, kanamycin, neomycin and streptomycin （each at a concentration of 600 mg/L） for 3 days; they were then transferred to antibiotics-free medium for 5 days; and finally they were spread on solid f/2 media to allow algal colonies to form. With this method, five strains ofNannochloropsis sp. （Eustigmatophyceae）, two strains of Cylindrotheca sp. （Bacillariophyceae）, two strains of Tetraselmis sp. （Chlorodendrophyceae） and one strain ofAmphikrikos sp. （Trebouxiophyceae） were purified successfully. The method shows promise for batch- purifying microalgal cultures.
