An attempt was made to preserve mango slices by treating with 2% calcium chloride solution followed by dipping into sugar syrup of different concentrations i.e. 50°, 60° and 70 °Brix respectively. The t...An attempt was made to preserve mango slices by treating with 2% calcium chloride solution followed by dipping into sugar syrup of different concentrations i.e. 50°, 60° and 70 °Brix respectively. The treated slices were subjected for drying in different modes of drying （oven, microwave oven and cabinet tray drying） and analyzed for various physico-chemical and organoleptic quality characteristics. The study revealed that the osmo-air dried slices of mango produced with partial dehydration facilitated by osmotic agent （sugar syrup of 60 °Brix. A fruit： sugar syrup ratio of 1：4 （w/v） for 18 h at 40 ℃ temperature） followed by mechanical drying showed superiority in sensorial quality attributes over other concentrations of ingredients and the rest of the modes of drying. The good quality osmotically dehydrated mango slices could be preserved with maximum retentions of vitamins with better dehydration, rehydration and sensorial quality characteristics.展开更多
[Objective] The aim was to study on effect of maltose concentration by ultrafiltration.[Method] 40% and 15% maltose syrups were concentrated using ultrafiltration membranes at molecular weight of 6 000 Da in order to ...[Objective] The aim was to study on effect of maltose concentration by ultrafiltration.[Method] 40% and 15% maltose syrups were concentrated using ultrafiltration membranes at molecular weight of 6 000 Da in order to study on concentration effect of maltose and restoration effect of membranes property through backflushing.[Result] When 40% maltose was concentrated with the membrane,membrane flux weakened rapidly and pores blocked.In contrast,when 15% maltose was concentrated with the same membrane,the membrane flux was high and weakened very slowly.Dextrose equivalent(DE) and luminousness changed from 43% and 91%T before filtration to 50% and 98%T after filtration,respectively.[Conclusion] The research provides a practical method to improve transparency of maltose products and extend quality guarantee period.展开更多
The aim of this study was to analyze the point mutation of the exon 1 at codon 54 of the mannose (or mannan)-binding lectin (MBL) gene in healthy individuals of Chinese Hans and Mongolian population, and to find out a...The aim of this study was to analyze the point mutation of the exon 1 at codon 54 of the mannose (or mannan)-binding lectin (MBL) gene in healthy individuals of Chinese Hans and Mongolian population, and to find out any association between the plasma levels of MBL and the gene mutation frequency in both groups of individuals. Blood samples were collected randomly from 56 healthy individuals of Chinese Hans and 37 Mongolian. The detection of the point mutations of the MBL gene was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and detections for plasma levels of MBL were determined by using MBL ELISA kits. A MBL PCR method of assay was established with high specificity, and good reproducibility. By optimizing the PCR condition, the optimal annealing temperature was 55℃, and the lowest detection limit was 160 pg. No bands were found in non-specificity samples (HAV, HBV, HCV and TB), and the sequences of PCR products were the same as the expected ones. Also a MBL PCR-RFLP was established. Upon electrophoresis of the digested products in 3% agarose gel, there were 3 patterns: in which 2 bands correspond to molecule weight 232 bp and 93 bp; 1 band, corresponds to molecule weight 325 bp and 3 bands correspond to molecule weight 325 bp, 232 bp and 93 bp, respectively. Three bands of 325 bp, 232 bp and 93 bp of point mutations were found at codon 54 of MBL coding gene. Frequencies in healthy Han and Mongolian population were 0.2321 and 0.1757 respectively. The average plasma MBL concentration was 1998.750 μg/L, with SD of 1505.152 in 56 healthy Han population and 2525.676 μg/L, with SD of 1955.188 in 37 Mongolian. A negative correlation between MBL concentration and gene mutation frequency was found in healthy Han population. Frequency of point mutation was 1.00 when the MBL concentrations were below 100 μg/L; frequency of point mutation was 0.4524 when the concentration was 100 μg/L to 1000 μg/L; and the frequency of point mutation was 0.0156 when the concentration was over 1000 μg/L. Analysis of association between MBL concentration and gene frequency in healthy Mongolian population showed that frequency of point mutation was 1.00 when the MBL concentrations were below 100 μg/L and the frequency of point mutation was 0.4583 when the MBL concentrations were 100 μg/L to 1000 μg/L; no point mutation was found when the concentration was over 1000 μg/L. It is concluded that the frequencies of mutation at codon 54 of MBL coding gene had been determined in both healthy Hans and Mongolian population, and the frequency was higher in healthy Hans than that of Mongolian, but no statistical significance (χ 2=0.8574, P >0.05). The MBL level was lower in healthy Hans than in Mongolian population, but there was no statistical significance( t =1.448, 0.1< P <0.2). There was a negative correlation between frequency of point mutation and MBL concentrations in both Hans and Mongolian population( r =-0.62, r =-0.641).展开更多
文摘An attempt was made to preserve mango slices by treating with 2% calcium chloride solution followed by dipping into sugar syrup of different concentrations i.e. 50°, 60° and 70 °Brix respectively. The treated slices were subjected for drying in different modes of drying （oven, microwave oven and cabinet tray drying） and analyzed for various physico-chemical and organoleptic quality characteristics. The study revealed that the osmo-air dried slices of mango produced with partial dehydration facilitated by osmotic agent （sugar syrup of 60 °Brix. A fruit： sugar syrup ratio of 1：4 （w/v） for 18 h at 40 ℃ temperature） followed by mechanical drying showed superiority in sensorial quality attributes over other concentrations of ingredients and the rest of the modes of drying. The good quality osmotically dehydrated mango slices could be preserved with maximum retentions of vitamins with better dehydration, rehydration and sensorial quality characteristics.
基金Supported by Demonstration Project of Ecology Restoration on PollutedWater Area in Estuary(2010BAC68B02)
文摘[Objective] The aim was to study on effect of maltose concentration by ultrafiltration.[Method] 40% and 15% maltose syrups were concentrated using ultrafiltration membranes at molecular weight of 6 000 Da in order to study on concentration effect of maltose and restoration effect of membranes property through backflushing.[Result] When 40% maltose was concentrated with the membrane,membrane flux weakened rapidly and pores blocked.In contrast,when 15% maltose was concentrated with the same membrane,the membrane flux was high and weakened very slowly.Dextrose equivalent(DE) and luminousness changed from 43% and 91%T before filtration to 50% and 98%T after filtration,respectively.[Conclusion] The research provides a practical method to improve transparency of maltose products and extend quality guarantee period.
文摘The aim of this study was to analyze the point mutation of the exon 1 at codon 54 of the mannose (or mannan)-binding lectin (MBL) gene in healthy individuals of Chinese Hans and Mongolian population, and to find out any association between the plasma levels of MBL and the gene mutation frequency in both groups of individuals. Blood samples were collected randomly from 56 healthy individuals of Chinese Hans and 37 Mongolian. The detection of the point mutations of the MBL gene was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and detections for plasma levels of MBL were determined by using MBL ELISA kits. A MBL PCR method of assay was established with high specificity, and good reproducibility. By optimizing the PCR condition, the optimal annealing temperature was 55℃, and the lowest detection limit was 160 pg. No bands were found in non-specificity samples (HAV, HBV, HCV and TB), and the sequences of PCR products were the same as the expected ones. Also a MBL PCR-RFLP was established. Upon electrophoresis of the digested products in 3% agarose gel, there were 3 patterns: in which 2 bands correspond to molecule weight 232 bp and 93 bp; 1 band, corresponds to molecule weight 325 bp and 3 bands correspond to molecule weight 325 bp, 232 bp and 93 bp, respectively. Three bands of 325 bp, 232 bp and 93 bp of point mutations were found at codon 54 of MBL coding gene. Frequencies in healthy Han and Mongolian population were 0.2321 and 0.1757 respectively. The average plasma MBL concentration was 1998.750 μg/L, with SD of 1505.152 in 56 healthy Han population and 2525.676 μg/L, with SD of 1955.188 in 37 Mongolian. A negative correlation between MBL concentration and gene mutation frequency was found in healthy Han population. Frequency of point mutation was 1.00 when the MBL concentrations were below 100 μg/L; frequency of point mutation was 0.4524 when the concentration was 100 μg/L to 1000 μg/L; and the frequency of point mutation was 0.0156 when the concentration was over 1000 μg/L. Analysis of association between MBL concentration and gene frequency in healthy Mongolian population showed that frequency of point mutation was 1.00 when the MBL concentrations were below 100 μg/L and the frequency of point mutation was 0.4583 when the MBL concentrations were 100 μg/L to 1000 μg/L; no point mutation was found when the concentration was over 1000 μg/L. It is concluded that the frequencies of mutation at codon 54 of MBL coding gene had been determined in both healthy Hans and Mongolian population, and the frequency was higher in healthy Hans than that of Mongolian, but no statistical significance (χ 2=0.8574, P >0.05). The MBL level was lower in healthy Hans than in Mongolian population, but there was no statistical significance( t =1.448, 0.1< P <0.2). There was a negative correlation between frequency of point mutation and MBL concentrations in both Hans and Mongolian population( r =-0.62, r =-0.641).
