本研究借助低场核磁共振技术(low field nuclear magnetic resonance,LF-NMR)测定经不同电子束辐照(electron beam irradiation,EBI)剂量处理后的大豆浓缩蛋白粉的水分分布信息,并通过热变性实验和傅里叶变换红外光谱(Fourier transform...本研究借助低场核磁共振技术(low field nuclear magnetic resonance,LF-NMR)测定经不同电子束辐照(electron beam irradiation,EBI)剂量处理后的大豆浓缩蛋白粉的水分分布信息,并通过热变性实验和傅里叶变换红外光谱(Fourier transform infrared,FT-IR)初步探究了EBI对大豆浓缩蛋白粉水分变化的影响及其变化机制。结果表明,大豆浓缩蛋白粉中的主要水组分为与大豆蛋白分子紧密结合的结合水(T_(21),占97.0%±0.7%)。随着EBI剂量的增加,结合水和总水分含量均先增大后减小,在5.40 kGy时达到最大值。经热变性实验和FT-IR光谱图分析得知EBI处理不会改变大豆浓缩蛋白粉的变性温度,但是能引起结合水中的O-H基团与氨基酸中的C=O所形成的分子内H键以及分子间H键振动增强。这些结果不仅表明EBI可通过改变大豆蛋白分子内和分子间的H键作用来影响其水分分布及含量,还为进一步探究经EBI处理的蛋白类食品中的水分迁移机制奠定了基础。展开更多
Objective To explore the role of serum fibrotic indices including hyaluronic acid （HA）, procollagen type Ⅲ NH2-terminal peptide （PCIIIP）, and laminin （LN） in assessing the severity of myocardial fibrosis in chr...Objective To explore the role of serum fibrotic indices including hyaluronic acid （HA）, procollagen type Ⅲ NH2-terminal peptide （PCIIIP）, and laminin （LN） in assessing the severity of myocardial fibrosis in chronic congestive heart failure （CHF）. Methods Serum levels of HA, PCIIIP, and LN in 39 patients with CHF E [14 with New York Heart Association （NYHA） functional class II, 21 with class Ⅲ, 4 with class Ⅳ] and in 46 patients with NYHA functional class I were assessed by radioimmunoassay. Results The serum concentrations of HA, PCMP, and LN were 359.75 ± 84.59 μg/L, 77.88 ± 24. 67 μg/L, 86. 73 ± 23.90 μg/L in CHF group, and 211.60 ±54. 80 μg/L, 64.82 ±23.99 μg/L, 82. 26 ±23.98 μg/L in NYHA functional class Ⅰ group, respectively. The HA level was significantly higher in CHF patients as compared with NYHA functional class Ⅰ group （ P 〈 0.05 ）. However, no difference was found in the levels of PCIIIP and LN between CHF group and NYHA functional class Ⅰ group. The serum HA concentration was negatively correlated with left ventricular ejection fraction （ r = - 0.71, P 〈 0.05 ）. Conclusion Serum HA level may act as an indicator for myocardial fibrosis.展开更多
Peformance enhancing drugs are widely used today in different sport fields, and therefore the successful anti-doping activity should be based not only on regular doping controls, but also it should show the real alter...Peformance enhancing drugs are widely used today in different sport fields, and therefore the successful anti-doping activity should be based not only on regular doping controls, but also it should show the real alternative： how to replace the forbidden doping substances with legal and effective supplements. The paper deals with application proposition of protein concentrates, free AA （amino acids）, HMB （hydroxy-methyle-butyrate）, creatine and camitine. If the athlete is involved in strength and power sport （e.g. throwing events in track and field, olympic weightlifting etc.）, the explosive and maximum strength is of primary importance. For strength athletes, these mentioned substances are those legal preparates, supplements, which can help effectively in performance improvement of sport results. The main reason is that using protein concentrates, AAs, HMB, creatine and camitine, the anabolic （and anticatabolic） effect will enhance the protein biosynthesis of the organism, improve the aerob and anaerob capacity of the athletes, activate and stimulate the own hormonal system. And the final result is creating higher loadability because of the faster recovery, and higher performance level on the competition. Of course, the athletes--as a minimum requirement--need an adequate nutrition （good balanced nourishment）, as well, with appropriate application and supplementation of all vitamins and essential minerals.展开更多
The objective of this stud）, was to valorize bovine and ovine cheese whey resulting from small and medium cheese manufacture plants, by producing liquid and dr）＂ whey protein concentrates （LWPC and WPC）. The flex...The objective of this stud）, was to valorize bovine and ovine cheese whey resulting from small and medium cheese manufacture plants, by producing liquid and dr）＂ whey protein concentrates （LWPC and WPC）. The flexibility implemented by batch ultrafiltration （UF） and diafiltration （DF） allowed for the production and ovine WPC with 61 -87% （dry basis）. Diatiltration, performed in of liquid bovine WPC with 43-66% protein contents （dry basis） sequential dilution mode （DFsdm） did not improve significantly the composition of WPC liquid products comparing to the results achieved by conventional UF. However, using DF in volume reduction mode （DFvrm） protein content was increased more than 20% comparing to conventional UF. Ovine products showed higher protein levels （62-84% dry basis） what makes their manufacture attractive. Protein profiles varied with the whey origin and with the concentration process. Using batch DFvrm was possible to obtain richer protein products free of low MW compounds.展开更多
文摘本研究借助低场核磁共振技术(low field nuclear magnetic resonance,LF-NMR)测定经不同电子束辐照(electron beam irradiation,EBI)剂量处理后的大豆浓缩蛋白粉的水分分布信息,并通过热变性实验和傅里叶变换红外光谱(Fourier transform infrared,FT-IR)初步探究了EBI对大豆浓缩蛋白粉水分变化的影响及其变化机制。结果表明,大豆浓缩蛋白粉中的主要水组分为与大豆蛋白分子紧密结合的结合水(T_(21),占97.0%±0.7%)。随着EBI剂量的增加,结合水和总水分含量均先增大后减小,在5.40 kGy时达到最大值。经热变性实验和FT-IR光谱图分析得知EBI处理不会改变大豆浓缩蛋白粉的变性温度,但是能引起结合水中的O-H基团与氨基酸中的C=O所形成的分子内H键以及分子间H键振动增强。这些结果不仅表明EBI可通过改变大豆蛋白分子内和分子间的H键作用来影响其水分分布及含量,还为进一步探究经EBI处理的蛋白类食品中的水分迁移机制奠定了基础。
文摘Objective To explore the role of serum fibrotic indices including hyaluronic acid （HA）, procollagen type Ⅲ NH2-terminal peptide （PCIIIP）, and laminin （LN） in assessing the severity of myocardial fibrosis in chronic congestive heart failure （CHF）. Methods Serum levels of HA, PCIIIP, and LN in 39 patients with CHF E [14 with New York Heart Association （NYHA） functional class II, 21 with class Ⅲ, 4 with class Ⅳ] and in 46 patients with NYHA functional class I were assessed by radioimmunoassay. Results The serum concentrations of HA, PCMP, and LN were 359.75 ± 84.59 μg/L, 77.88 ± 24. 67 μg/L, 86. 73 ± 23.90 μg/L in CHF group, and 211.60 ±54. 80 μg/L, 64.82 ±23.99 μg/L, 82. 26 ±23.98 μg/L in NYHA functional class Ⅰ group, respectively. The HA level was significantly higher in CHF patients as compared with NYHA functional class Ⅰ group （ P 〈 0.05 ）. However, no difference was found in the levels of PCIIIP and LN between CHF group and NYHA functional class Ⅰ group. The serum HA concentration was negatively correlated with left ventricular ejection fraction （ r = - 0.71, P 〈 0.05 ）. Conclusion Serum HA level may act as an indicator for myocardial fibrosis.
文摘Peformance enhancing drugs are widely used today in different sport fields, and therefore the successful anti-doping activity should be based not only on regular doping controls, but also it should show the real alternative： how to replace the forbidden doping substances with legal and effective supplements. The paper deals with application proposition of protein concentrates, free AA （amino acids）, HMB （hydroxy-methyle-butyrate）, creatine and camitine. If the athlete is involved in strength and power sport （e.g. throwing events in track and field, olympic weightlifting etc.）, the explosive and maximum strength is of primary importance. For strength athletes, these mentioned substances are those legal preparates, supplements, which can help effectively in performance improvement of sport results. The main reason is that using protein concentrates, AAs, HMB, creatine and camitine, the anabolic （and anticatabolic） effect will enhance the protein biosynthesis of the organism, improve the aerob and anaerob capacity of the athletes, activate and stimulate the own hormonal system. And the final result is creating higher loadability because of the faster recovery, and higher performance level on the competition. Of course, the athletes--as a minimum requirement--need an adequate nutrition （good balanced nourishment）, as well, with appropriate application and supplementation of all vitamins and essential minerals.
文摘The objective of this stud）, was to valorize bovine and ovine cheese whey resulting from small and medium cheese manufacture plants, by producing liquid and dr）＂ whey protein concentrates （LWPC and WPC）. The flexibility implemented by batch ultrafiltration （UF） and diafiltration （DF） allowed for the production and ovine WPC with 61 -87% （dry basis）. Diatiltration, performed in of liquid bovine WPC with 43-66% protein contents （dry basis） sequential dilution mode （DFsdm） did not improve significantly the composition of WPC liquid products comparing to the results achieved by conventional UF. However, using DF in volume reduction mode （DFvrm） protein content was increased more than 20% comparing to conventional UF. Ovine products showed higher protein levels （62-84% dry basis） what makes their manufacture attractive. Protein profiles varied with the whey origin and with the concentration process. Using batch DFvrm was possible to obtain richer protein products free of low MW compounds.
