γ-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter, and the GABAergic synaptic transmission is normally terminated by the rapid uptake through GABA transporters. With transgenic mice ubiquitously...γ-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter, and the GABAergic synaptic transmission is normally terminated by the rapid uptake through GABA transporters. With transgenic mice ubiquitously overexpressing GABA transporter subtype I (GAT1), the present study explored the pathophysiological role of GAT1 in epileptogenesis. Though displaying no spontaneous seizure activity, these mice exhibit altered electroencephalographic patterns and increased susceptibility to seizure induced by kainic acid. In addition, the GABAA receptor and glutamate transporters are up-regulated in transgenic mice, which perhaps reflects a compensatory or corrective change to the elevated level of GAT1. These preliminary findings support the hypothesis that excitatory and inhibitory neurotransmission, and seizure susceptibility can be altered by neurotransmitter transporters.展开更多
The present paper studies the sedimentary characteristics and mechanism of the shell ridges on the southwestern coast of Bohai Bay, which are the largest and have the highest shell (or shell fragment) content in the w...The present paper studies the sedimentary characteristics and mechanism of the shell ridges on the southwestern coast of Bohai Bay, which are the largest and have the highest shell (or shell fragment) content in the world. These shell ridges are composed of two sedimentary subfacies: the ridge subfacies and the ridge infill subfacies with different inner textures and sedimentary structures. The ridge subfacies primarily consists of fresh shells and/or shell fragments with parallel beddings and high-angle oblique beddings. The ridge infill subfacies consists of finer shell fragments and silty sand with parallel beddings oblique to land. The evolution of the shell ridge is controlled by the accumulation of substantial shell material,the relative slow erosion of shoreline, storm waves, winds and the shift of river routes. Wind tunnel tests indicate that the critical movement velocity of shell is lower than that of quartz sand of the same grain size. Deltaic progradation alternates with shell ridge growth. While the Yellow River empties into the sea through this area, accompanied by deltaic progradating, the shell material is scarce, which is unfavorable to the growth of the shell ridge. Conversely, erosion occurs along an abandoned delta coast where a shell ridge may develop, e.g. the shell ridge on the southwest of Bohai Bay.展开更多
Objective. To examine the effect of isobutyramide synthesized in our laboratory on human and murine globin gene expression and to test cell toxicity ofthe drug.Methods. MEL cells were transfected with the recombinant ...Objective. To examine the effect of isobutyramide synthesized in our laboratory on human and murine globin gene expression and to test cell toxicity ofthe drug.Methods. MEL cells were transfected with the recombinant construct μLCRAγψβδβand the stable transformants were cultured in the medium with different concentrations of isobutyramide. The experimental mice and rabbit were injected with different doses of isobutyramide. The globin mRNAs were analyzed by RNase protection assay. The hematological toxicity and electrolyte toxicity ofthe drug were tested.Results. An inducible and dose dependent expression of the human γ , β and mouse α globin gene was observed in the transfected MEL cells. The induction of the human γ globin gene is significant stronger than that of the β globin gene. With 2.5～5 mmol/L isobutyramide, the induction of the human γ globin gene is even more effective than that of mouse α globin gene. After a 15 day injection under the doses of 500～900mg·kg-1·d-1, the level of the mouse embryonic εy globin mRNA could be significantly induced up to 3～4 fold of that of uninjected controls. The changes of hemoglobin(Hb), RBC, hematocrit(HCT), WBC, derived from mice injected with different doses of isobutyramide at the interval of 24 hours for 2～4 weeks, were generally within the normal range. In rabbits injected with isobutyramide in the same regiment for 2 weeks, the concentration of blood K+, Na+, Cl-and CO2 were all within normal range and serum ionic osmotic pressure remained stable as well. Conclusion. Our results suggested that isobutyramide is a weak inducer ofcell differentiation, but it can selectively activate transcription of human γ globin gene at a certain degree, and it can act on early stages of erythroid progenitor differentiation in adult mice and activate transcription of embryonic εy-globin gene and have no hematological toxicity. Our results have further proved the potential value of isobutyramide in treatment of β-thalassemia and sickle cell disease.展开更多
This paper presents research aimed at identifying the methods with which prehistoric man interacted with marine and inland water environments. The objective is to determine the degree to which resources from these env...This paper presents research aimed at identifying the methods with which prehistoric man interacted with marine and inland water environments. The objective is to determine the degree to which resources from these environments influenced the socio-economic organization of these peoples. The coastline around Cagliari and its hinterland （southern Sardinia, Italy） were chosen as the subject, on the basis of the presence of both the sea and several ponds. The territory is noted as being home to both Neolithic and Eneolithic settlements. Among these the archaeological excavation of the village of Su Coddu/Canelles produced a large quantity of malacofauna. Data has been processed from part of the settlement that gave radiocarbon dating from the period between 3640 and 2900 cal. BC, and in which it was possible to carry out the level of sampling that was necessary to complete the research. In particular this interdisciplinary project, which aims to make an overall study of the settlement, the data relating to the geography of the area, the archaeological documentation, the fauna and shell artefacts will be extrapolated. Numerous methods are applied above all in reference to the marines and ponds resources. Additionally, the morphological anatomical-comparative analysis （in order to determine the typology of the faunal remains and to identify each individual species） and the technological analysis of the manufactured shells （so as to detect the selection and the transformation type of raw materials） are accomplished. The research has led to the identification of a settlement model in which agriculture formed the primary means of subsistence, that was complemented by the rearing of livestock, through hunting and gathering. The exploitation of marine and inland waters resources, as food source, have to be important because 99% of the faunal remains are shells. Different shell species are recognized, among those the Ostrea edulis provides the raw material to produce bevelled objects, which are documented inside the settlement in considerable number. Some plant species, typical of the lagoon ecosystem, were used for building and craft production.展开更多
文摘γ-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter, and the GABAergic synaptic transmission is normally terminated by the rapid uptake through GABA transporters. With transgenic mice ubiquitously overexpressing GABA transporter subtype I (GAT1), the present study explored the pathophysiological role of GAT1 in epileptogenesis. Though displaying no spontaneous seizure activity, these mice exhibit altered electroencephalographic patterns and increased susceptibility to seizure induced by kainic acid. In addition, the GABAA receptor and glutamate transporters are up-regulated in transgenic mice, which perhaps reflects a compensatory or corrective change to the elevated level of GAT1. These preliminary findings support the hypothesis that excitatory and inhibitory neurotransmission, and seizure susceptibility can be altered by neurotransmitter transporters.
基金part of which has been presented here,are sponsored by the National Natural Science Foundation of China(No.40246026).
文摘The present paper studies the sedimentary characteristics and mechanism of the shell ridges on the southwestern coast of Bohai Bay, which are the largest and have the highest shell (or shell fragment) content in the world. These shell ridges are composed of two sedimentary subfacies: the ridge subfacies and the ridge infill subfacies with different inner textures and sedimentary structures. The ridge subfacies primarily consists of fresh shells and/or shell fragments with parallel beddings and high-angle oblique beddings. The ridge infill subfacies consists of finer shell fragments and silty sand with parallel beddings oblique to land. The evolution of the shell ridge is controlled by the accumulation of substantial shell material,the relative slow erosion of shoreline, storm waves, winds and the shift of river routes. Wind tunnel tests indicate that the critical movement velocity of shell is lower than that of quartz sand of the same grain size. Deltaic progradation alternates with shell ridge growth. While the Yellow River empties into the sea through this area, accompanied by deltaic progradating, the shell material is scarce, which is unfavorable to the growth of the shell ridge. Conversely, erosion occurs along an abandoned delta coast where a shell ridge may develop, e.g. the shell ridge on the southwest of Bohai Bay.
文摘Objective. To examine the effect of isobutyramide synthesized in our laboratory on human and murine globin gene expression and to test cell toxicity ofthe drug.Methods. MEL cells were transfected with the recombinant construct μLCRAγψβδβand the stable transformants were cultured in the medium with different concentrations of isobutyramide. The experimental mice and rabbit were injected with different doses of isobutyramide. The globin mRNAs were analyzed by RNase protection assay. The hematological toxicity and electrolyte toxicity ofthe drug were tested.Results. An inducible and dose dependent expression of the human γ , β and mouse α globin gene was observed in the transfected MEL cells. The induction of the human γ globin gene is significant stronger than that of the β globin gene. With 2.5～5 mmol/L isobutyramide, the induction of the human γ globin gene is even more effective than that of mouse α globin gene. After a 15 day injection under the doses of 500～900mg·kg-1·d-1, the level of the mouse embryonic εy globin mRNA could be significantly induced up to 3～4 fold of that of uninjected controls. The changes of hemoglobin(Hb), RBC, hematocrit(HCT), WBC, derived from mice injected with different doses of isobutyramide at the interval of 24 hours for 2～4 weeks, were generally within the normal range. In rabbits injected with isobutyramide in the same regiment for 2 weeks, the concentration of blood K+, Na+, Cl-and CO2 were all within normal range and serum ionic osmotic pressure remained stable as well. Conclusion. Our results suggested that isobutyramide is a weak inducer ofcell differentiation, but it can selectively activate transcription of human γ globin gene at a certain degree, and it can act on early stages of erythroid progenitor differentiation in adult mice and activate transcription of embryonic εy-globin gene and have no hematological toxicity. Our results have further proved the potential value of isobutyramide in treatment of β-thalassemia and sickle cell disease.
文摘This paper presents research aimed at identifying the methods with which prehistoric man interacted with marine and inland water environments. The objective is to determine the degree to which resources from these environments influenced the socio-economic organization of these peoples. The coastline around Cagliari and its hinterland （southern Sardinia, Italy） were chosen as the subject, on the basis of the presence of both the sea and several ponds. The territory is noted as being home to both Neolithic and Eneolithic settlements. Among these the archaeological excavation of the village of Su Coddu/Canelles produced a large quantity of malacofauna. Data has been processed from part of the settlement that gave radiocarbon dating from the period between 3640 and 2900 cal. BC, and in which it was possible to carry out the level of sampling that was necessary to complete the research. In particular this interdisciplinary project, which aims to make an overall study of the settlement, the data relating to the geography of the area, the archaeological documentation, the fauna and shell artefacts will be extrapolated. Numerous methods are applied above all in reference to the marines and ponds resources. Additionally, the morphological anatomical-comparative analysis （in order to determine the typology of the faunal remains and to identify each individual species） and the technological analysis of the manufactured shells （so as to detect the selection and the transformation type of raw materials） are accomplished. The research has led to the identification of a settlement model in which agriculture formed the primary means of subsistence, that was complemented by the rearing of livestock, through hunting and gathering. The exploitation of marine and inland waters resources, as food source, have to be important because 99% of the faunal remains are shells. Different shell species are recognized, among those the Ostrea edulis provides the raw material to produce bevelled objects, which are documented inside the settlement in considerable number. Some plant species, typical of the lagoon ecosystem, were used for building and craft production.
