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Identification of AOSC-binding proteins in neurons
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作者 刘明 聂琴 +1 位作者 辛现良 耿美玉 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2008年第4期394-399,共6页
Acidic oligosaccharide sugar chain (AOSC), a D-mannuronic acid oligosaccharide, derived from brownalgaepolysaccharide, has been completed Phase I clinical trial in China as an anti-Alzheimer's Disease (AD) drug c... Acidic oligosaccharide sugar chain (AOSC), a D-mannuronic acid oligosaccharide, derived from brownalgaepolysaccharide, has been completed Phase I clinical trial in China as an anti-Alzheimer's Disease (AD) drug candidate. The identification of AOSC-binding protein(s) in neurons is very important for understanding its action mechanism. To determine the binding protein(s) of AOSC in neurons mediating its anti-AD activities, confocal microscopy, affinity chromatography, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis were used. Confocal microscopy analysis shows that AOSC binds to SH-SY5Y cells in concentration-, time-, and temperature-dependent fashions. The AOSC binding proteins were purified by affinity chromatography and identified by LC-MS/MS analysis. The results showed that there are 349 proteins binding AOSC, including clathrin, adaptor protein-2 (AP-2) and amyloid precursor protein (APP). These results suggest that the binding/entrance of AOSC to neurons is probably responsible for anti-AD activities. 展开更多
关键词 AOSC Alzheimer's disease marine oligosaccharide binding protein(s)
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