盐渍海带盐中可培养嗜盐古菌多样性、胞外酶活及拮抗作用探究

盐渍海带盐是一种高盐环境,其中可能生存有嗜盐微生物,如嗜盐古菌。嗜盐古菌是一类生活于高盐环境的极端环境微生物。为了探究盐渍海带盐中嗜盐微生物的物种多样性,筛查分离了菌株的几种常见胞外功能酶活性和拮抗活性。采用纯培养技术,从盐渍海带盐样品中分离培养嗜盐微生物,并对其16S rRNA基因进行扩增和测序;基于16S rRNA基因序列的相似度分析,确定分离菌株在属级水平的分类地位;依据种属信息,挑选代表菌株,进行胞外常见功能酶活性和拮抗作用测定。从盐渍海带盐样品中分离到来自Haloarcula(盐盒菌属)、Halorubrum(盐红菌属)、Halarchaeum、Halobacterium(盐杆菌属)、Halococcus(盐球菌属)、Halolamina(盐薄片形菌属)和Haloplanus(盐扁平菌属)等7个属的131株嗜盐古菌;检测到产胞外蛋白酶菌株1株,产酯酶菌2株,产明胶酶菌7株,产氧化酶菌1株和产触菌酶5株;此外,筛选到6株具有拮抗活性的菌株,其中来自盐红菌属菌株Halorubrum sp. ZSA68较其他菌株生长快,产抑菌活性物质快,并显示出较强的抑菌活性和较广的抑菌谱,初步推测Halorubrum sp. ZSA68所产物质为多肽或蛋白类抑菌活性物质,其分子量大小约为30~50 kDa,该抑菌活性物质在60℃以上或NaCl浓度低于2%时容易失去活性。通过本研究认识了海带盐中可培养嗜盐古菌的分布,获得多株具有较高胞外酶活性及具拮抗作用的菌株,积累了丰富的嗜盐古菌菌株资源,这在高盐食品的防腐方面具有潜在的应用价值。

Denitrification and Nitrate Reduction to Ammoniumin Taihu Lake and Yellow Sea Inter-Tidal MarineSediments

Denitrification and nitrate reduction to ammonium in Taihu Lake and Yellow Sea inter-tidal marinesediments were studied. The sediment samples were made into slurry containing 150 g dry matter per liter.Various amounts of glucose and 5 mmol L-1 of potassium nitrate were added in order to achieve differentratios of glucose-C to nitrate-N. Acetylene inhibition technique was applied to measure denitrification in theslumes. All samples were incubated anaerobically under argon atmosphere. Data showed that Taihu Lakesediment produced more N2O than marine sediment. Denitrification potential was higher in Taihu Lakesediment than in marne one. Glucose added increased denitrification activity but not the denitrification po-tential of the sediments. Dissimilatory nitrate reduction to ammonium seemed to occur in marine sediment,but not in freshwater one. When the marine sediment was treated with 25 mmol L-1 glucose, its denitrification poteatial, as indicated by maximum N2O production by acetylene blockage, was lower than that treatedwith no or 2.5 mmol L-l glucose. Acetylene was suspected to have inhibitory effect on dissimilatory nitratereduction to ammonium.

Freshening biases in the freshwater flux of CORE data

The authors investigate biases in the freshwater flux（FWF） of CORE.v2—a common data-set for stand-alone ocean models—based on the results of a set of experiments using an OGCM. The authors identify freshening biases in the FWF in the subtropical regions of the North Pacific, South Pacific, and South Atlantic, which may be caused by the weak surface wind, high specific humidity,or high precipitation in the CORE.v2 data. The authors also find biases in sea surface salinity that are caused by ocean dynamics, such as in the North Atlantic, and that cannot be corrected by correcting surface forcing.

Thermohaline finestructure observed near the northern Philippine coast

Using hydrographic measurements from three recent surveys in the western tropical Pacific, this study revealed the existence and general features of thermohaline finestructure near the northem Philippine coast. Pronounced finestructttres were detected in the layers of the North Pacific Tropical Water （NPTW） and the North Pacific Intermediate Water （NPIW） during all three cruises and shown to be mainly thermohaline intrusions. Characteristics of the intrusions were further investigated with spiciness curvature and salinity anomaly methods. The vertical scale of the intrusions was 20-50 m and 50-100 m in the NPTW and NPIW layers, respectively. Within the NPTW layer, the Turner angle distribution and correlation between salinity and density anomalies suggested that diffusive convection between surface fresh water and subsurface saline water played an important role in the development and maintenance of the intrusions. In addition, connection between thermohaline finestructure and larger-scale oceanic processes was explored using historical hydrographic data. The results reveal that the salinity field and the distribution of the intrusions in this region were largely determined by mesoscale eddies. As a result of eddy stirring, both isopycnal and diapycnal temperature/salinity gradients were strengthened, which gave rise to the development of thermohaline intrusions. The intrusions acted to enhance heat and salt fluxes and resulted in the mixing of water masses being more efficient. By linking mesoscale eddy stirring to micro-scale diffusion, thermohaline finestructure plays a vital role in the ocean energy cascade and water mass conversion in the northern Philippine Sea.

Ecological-sedimentary zonations and carbonate deposition,Xiaodonghai Reef Flat,Sanya,Hainan Island,China

Modern fringing reefs are developed on the intertidal to subtidal area of Xiaodonghai bay, Sanya, Hainan Island. The reef flat extends several dozen meters toward the sea. Various ecological and sedimentary zonations can be distinguished, including, from land to sea, beachrocks, large massive corals, inner reef flat, outer reef flat, and fore-reef slope. The carbonate sediments and constituent hermatypic coral communities are different in these zones. The beachrocks are composed mainly of biological sands, including coral skeletons, algae, gastropods, bivalves, and benthic foraminifera. Quartz sands are less common in this zone. Carbonate diagenesis in beachrocks is characterized by early cementation. The fringing reef flat is progradationally developed toward the sea. Large massive corals grew in the zone next to the beachrocks. These corals are large, flat-toped, and autochthonous in the production of bioclastic grains and lime mud. Large and massive coral skeletons are surrounded by coral skeletal grains, bioclasts, and lime-mud sediments. The inner reef flat consists mainly of skeletons of branching corals that are covered by bioclasts and lime mud, and living corals are mostly massive in form. Statistical analysis of coral-community dynamics shows that the outer reef flat with strong waves is the most suitable place for hermatypic corals to flourish. There, the living corals are most densely distributed. Both massive corals and branching corals (e.g., Acropora corymbosa) are exceptionally healthy in this zone. Bioclasts and lime mud are washed out due to strong wave action. Microbial carbonates generally are deposited within cavities of the beachrocks and coral skeletal cavities and on the surfaces of bioclastic grains. The latter two types, however, are more common. Ecological-sedimentary zonations across the Xiaodonghai reef flat from beachrocks to outer reef flat are controlled by variations in wave strength and water energy. Microbial carbonates rarely developed on outer reef flat under strong wave action.