Skeletonema tropicum is regarded as a species with an affinity to warm waters and it has never been reported in seas where temperatures drop below 11℃ in winter. Previous studies in China reported that S. tropicum wa...Skeletonema tropicum is regarded as a species with an affinity to warm waters and it has never been reported in seas where temperatures drop below 11℃ in winter. Previous studies in China reported that S. tropicum was restricted to subtropical and warm temperate seas （East and South China Seas）, but the species was recently found during August cruises of 2009 and 2010 in Jiaozhou Bay, Yellow Sea, located several hundred kilometers to the north. Here, winter water temperatures often drop below 5℃. Identification of S. tropicum was confirmed under light and scanning electronic microscopes and maximum cell abundance in Jiaozhou Bay was estimated as 1.73× 10^4 celI/L. This record of S. tropicum in Jiaozhou Bay represents a significant northward expansion in the geographic range of the species. Ship ballast water was identified as a possible carrier of S. tropicum from southern places along Chinese coastline, and in addition, thermal pollution from local power stations and seawater desalination plants may provide suitable conditions for species over-wintering.展开更多
Rapid,high-throughput and reliable methods are urgently required to accurately detect and monitor harmful algae,which are responsible for algal blooms,such as red and green tides. In this study,we successfully develop...Rapid,high-throughput and reliable methods are urgently required to accurately detect and monitor harmful algae,which are responsible for algal blooms,such as red and green tides. In this study,we successfully developed a multiplex PCR-based DNA microarray method capable of detecting nine harmful algal species simultaneously,namely A lexandrium tamarense,Gyrodinium instriatum,Heterosigma akashiwo,Karenia mikimotoi,Prorocentrum donghaiense,Prorocentrum minimum,Ulva compressa,Ulva ohnoi and Ulva prolifera. This method achieved a limit of detection(LOD) of 0.5 ng of genomic DNA(orders of magnitude of the deci-nanogram range) in the tested algae cultures. Altogether,230 field samples from ship ballast waters and seaport waters were used to evaluate the DNA microarray. The clinical sensitivity and specificity of the DNA microarray assay in detecting field samples were 96.4% and 90.9%,respectively,relative to conventional morphological methods. This indicated that this high-throughput,automatic,and specific method is well suited for the detection of algae in water samples.展开更多
The pioneer technical policy carried out by Federal State Institution ＂Novorossiysk Maritime Port Administration＂ in mutual cooperation with the Southern Scientific Centre of Russian Academy of Sciences on control a...The pioneer technical policy carried out by Federal State Institution ＂Novorossiysk Maritime Port Administration＂ in mutual cooperation with the Southern Scientific Centre of Russian Academy of Sciences on control and management of ballast waters and ecosystem monitoring of marine environment in areas of ballast water discharge provides the task of complex approach for minimization of risk of introduction of biologically negative invaders by marine transport and preservation of biodiversity of the Black Sea.展开更多
基金Supported by the National Natural Science Foundation of China (Nos.40976097, 41006040)the Strategic Priority Research Program of Chinese Academy of Sciences (No. XDA05130703)the Marine Special Scientific Fund for Non-profit Public Industry (No. 200805031)
文摘Skeletonema tropicum is regarded as a species with an affinity to warm waters and it has never been reported in seas where temperatures drop below 11℃ in winter. Previous studies in China reported that S. tropicum was restricted to subtropical and warm temperate seas （East and South China Seas）, but the species was recently found during August cruises of 2009 and 2010 in Jiaozhou Bay, Yellow Sea, located several hundred kilometers to the north. Here, winter water temperatures often drop below 5℃. Identification of S. tropicum was confirmed under light and scanning electronic microscopes and maximum cell abundance in Jiaozhou Bay was estimated as 1.73× 10^4 celI/L. This record of S. tropicum in Jiaozhou Bay represents a significant northward expansion in the geographic range of the species. Ship ballast water was identified as a possible carrier of S. tropicum from southern places along Chinese coastline, and in addition, thermal pollution from local power stations and seawater desalination plants may provide suitable conditions for species over-wintering.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA092001)the Science and Technology Project of Zhejiang Province(No.2013C03045-1)+2 种基金the Zhejiang Marine Biotechnology Innovation Team(No.2010R50029-12)the Natural Science Foundation of Ningbo City of China(No.2013A610168)the KC Wong Magna Fund in Ningbo University
文摘Rapid,high-throughput and reliable methods are urgently required to accurately detect and monitor harmful algae,which are responsible for algal blooms,such as red and green tides. In this study,we successfully developed a multiplex PCR-based DNA microarray method capable of detecting nine harmful algal species simultaneously,namely A lexandrium tamarense,Gyrodinium instriatum,Heterosigma akashiwo,Karenia mikimotoi,Prorocentrum donghaiense,Prorocentrum minimum,Ulva compressa,Ulva ohnoi and Ulva prolifera. This method achieved a limit of detection(LOD) of 0.5 ng of genomic DNA(orders of magnitude of the deci-nanogram range) in the tested algae cultures. Altogether,230 field samples from ship ballast waters and seaport waters were used to evaluate the DNA microarray. The clinical sensitivity and specificity of the DNA microarray assay in detecting field samples were 96.4% and 90.9%,respectively,relative to conventional morphological methods. This indicated that this high-throughput,automatic,and specific method is well suited for the detection of algae in water samples.
文摘The pioneer technical policy carried out by Federal State Institution ＂Novorossiysk Maritime Port Administration＂ in mutual cooperation with the Southern Scientific Centre of Russian Academy of Sciences on control and management of ballast waters and ecosystem monitoring of marine environment in areas of ballast water discharge provides the task of complex approach for minimization of risk of introduction of biologically negative invaders by marine transport and preservation of biodiversity of the Black Sea.
