Objective: To investigate the effects of seawater immersion on the function of myocardium and hepatocyte mitochondria in experimental hemorrhagic shock rats. Methods: Twenty-four male Wistar rats were divided into thr...Objective: To investigate the effects of seawater immersion on the function of myocardium and hepatocyte mitochondria in experimental hemorrhagic shock rats. Methods: Twenty-four male Wistar rats were divided into three groups (n=8 in each group): control group, HSL group (hemorrhagic shock group on land) and HSS group (hemorrhagic shock group in seawater). The hemodynamic parameters, activities of H+-ATPase (adenosinetriphosphatase), succinate dehydrogenase (SDH) and Ca 2+-Mg 2+-ATPase, the calcium contents in myocardium and hepatocyte mitochondria were measured and the changes of proton translocation across the inner mitochondrial membrane were analyzed. Results: The hemodynamic indexes and the activities of H+-ATPase, SDH, Ca 2+-Mg 2+-ATPase in HSS group were significantly lower than those in control group and HSL group (P< 0.05). In HSS group the calcium levels in tissue and mitochondria of myocardium and hepatocyte were elevated significantly compared with control group and HSL group(P< 0.05). There was no significant difference in proton translocation among three groups. Conclusions: This investigation demonstrates that seawater immersion can aggravate the conditions of hemorrhagic shock rats.展开更多
文摘Objective: To investigate the effects of seawater immersion on the function of myocardium and hepatocyte mitochondria in experimental hemorrhagic shock rats. Methods: Twenty-four male Wistar rats were divided into three groups (n=8 in each group): control group, HSL group (hemorrhagic shock group on land) and HSS group (hemorrhagic shock group in seawater). The hemodynamic parameters, activities of H+-ATPase (adenosinetriphosphatase), succinate dehydrogenase (SDH) and Ca 2+-Mg 2+-ATPase, the calcium contents in myocardium and hepatocyte mitochondria were measured and the changes of proton translocation across the inner mitochondrial membrane were analyzed. Results: The hemodynamic indexes and the activities of H+-ATPase, SDH, Ca 2+-Mg 2+-ATPase in HSS group were significantly lower than those in control group and HSL group (P< 0.05). In HSS group the calcium levels in tissue and mitochondria of myocardium and hepatocyte were elevated significantly compared with control group and HSL group(P< 0.05). There was no significant difference in proton translocation among three groups. Conclusions: This investigation demonstrates that seawater immersion can aggravate the conditions of hemorrhagic shock rats.
