The effects of marine environmental factors-temperature （T）, dissolved oxygen （DO）, salinity （S） and pH--on the oxidation-reduction potential （ORP） of natural seawater were studied in laboratory. The results s...The effects of marine environmental factors-temperature （T）, dissolved oxygen （DO）, salinity （S） and pH--on the oxidation-reduction potential （ORP） of natural seawater were studied in laboratory. The results show an indistinct relationship between these four factors and the ORE but they did impact the ORP. Common mathematical methods were not applicable for describing the relationship. Therefore, a grey relational analysis （GRA） method was developed. The degrees of correlation were calculated according to GILA and the values of T, pH, DO and S were 0.744, 0.710, 0.692 and 0.690, respectively. From these values, the relations of these factors to the ORP could be described and evaluated, and those of T and pH were relatively major. In general, ORP is influenced by the synergic effect of T, DO, pH and S, with no single factor having an outstanding role.展开更多
A group of coenocytic marine algae differs from higher plants,whose totipotency depends on an intact cell(or protoplast).Instead,this alga is able to aggregate its extruded protoplasm in sea water and generate new mat...A group of coenocytic marine algae differs from higher plants,whose totipotency depends on an intact cell(or protoplast).Instead,this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals.It is thought that lectins play a key role in the aggregation process.We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides.The lectin was ca.27 kDa with a pI between pH 5 and pH 6.The absence of carbohydrate suggested that the lectin was not a glycoprotein.The hemagglutinating activity(HA) of the lectin was not dependent on the presence of divalent cations and was inhibited by N-Acetylgalactosamine,N-Acetylglucosamine,and the glycoprotein bovine submaxillary mucin.The lectin preferentially agglutinated Gram-negative bacterium.The HA of this lectin was stable between pH 4 to pH 10.Cell organelles outside the cytoplasm were agglutinated by the addition of lectin solution(0.5 mg ml-1).Our results suggest that the regeneration of B.hypnoides is mediated by this lectin.We also demonstrated that the formation of cell organelle aggregates was inhibited by nigericin in natural seawater(pH 8.0).Given that nigericin dissipates proton gradients across the membrane,we hypothesize that the aggregation of cell organelles was proton-gradient dependent.展开更多
Over the past few years, harmful algal blooms （HABs）, such as red tides, have been frequently observed in coastal zones worldwide. The natural allelopathic interactions among macroalgae and red tide microalgae can a...Over the past few years, harmful algal blooms （HABs）, such as red tides, have been frequently observed in coastal zones worldwide. The natural allelopathic interactions among macroalgae and red tide microalgae can alter the structure and succession of aquatic ecosystems. We investigated the influence of four environmental factors （temperature, salinity, light, and pH） on the allelopathic effects of the macroalgae Corallina pilulifera on red-tide forming Heterosigma akashiwo under laboratory conditions. Each of the factors had four levels： temperature （15, 20, 25, and 30℃）, salinity （10, 20, 30, and 40）, light （20, 100, 200 and 400 gmol/（m2.s））, and pH （5.5, 7, 8.5, and 10. Two-factor experiments were designed for each two environmental factors, with six combination treatments （temperature-salinity, temperature-light, temperature-pH, salinity-light, salinity-pH, and light-pH）. Results showed that the allelopathic effect was significantly influenced by temperature, salinity, light, and pH. As single factors, the low temperature （15 ℃）, low salinity （10）, high-intensity light （400umol/（m2.s））, and high pH （10） treatments substantially enhanced the allelopathic effect. The strongest allelopathic effect of C. pilulifera on H. akashiwo was observed under the following treatments： 15℃ and salinity of 40, 25℃ and pH 10, 25℃ with medium- to high-intensity light at 200-400 umol/（m2.s）, 400umol/（m2.s） and salinity of 10, 400 gmol/（m2·s） and pH 10, and pH 10 with a salinity of 40.展开更多
基金Supporte by the Knowledge Innovation Project of the Chinese Academy of Sciences (No KZCX2-YW-210)National Key Technology Research and Development Program (No2007BAB27B04)the High Technology Research and Development Program of China (No 2001AA635080)
文摘The effects of marine environmental factors-temperature （T）, dissolved oxygen （DO）, salinity （S） and pH--on the oxidation-reduction potential （ORP） of natural seawater were studied in laboratory. The results show an indistinct relationship between these four factors and the ORE but they did impact the ORP. Common mathematical methods were not applicable for describing the relationship. Therefore, a grey relational analysis （GRA） method was developed. The degrees of correlation were calculated according to GILA and the values of T, pH, DO and S were 0.744, 0.710, 0.692 and 0.690, respectively. From these values, the relations of these factors to the ORP could be described and evaluated, and those of T and pH were relatively major. In general, ORP is influenced by the synergic effect of T, DO, pH and S, with no single factor having an outstanding role.
基金Supported by the Natural Science Foundation of China (Nos 40806063,30830015)the National High Technology Research and Development Program of China (863 Program) (Nos2007AA09Z406,2006AA10A413)
文摘A group of coenocytic marine algae differs from higher plants,whose totipotency depends on an intact cell(or protoplast).Instead,this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals.It is thought that lectins play a key role in the aggregation process.We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides.The lectin was ca.27 kDa with a pI between pH 5 and pH 6.The absence of carbohydrate suggested that the lectin was not a glycoprotein.The hemagglutinating activity(HA) of the lectin was not dependent on the presence of divalent cations and was inhibited by N-Acetylgalactosamine,N-Acetylglucosamine,and the glycoprotein bovine submaxillary mucin.The lectin preferentially agglutinated Gram-negative bacterium.The HA of this lectin was stable between pH 4 to pH 10.Cell organelles outside the cytoplasm were agglutinated by the addition of lectin solution(0.5 mg ml-1).Our results suggest that the regeneration of B.hypnoides is mediated by this lectin.We also demonstrated that the formation of cell organelle aggregates was inhibited by nigericin in natural seawater(pH 8.0).Given that nigericin dissipates proton gradients across the membrane,we hypothesize that the aggregation of cell organelles was proton-gradient dependent.
基金Supported by the National Natural Science Foundation of China(No.31200400)
文摘Over the past few years, harmful algal blooms （HABs）, such as red tides, have been frequently observed in coastal zones worldwide. The natural allelopathic interactions among macroalgae and red tide microalgae can alter the structure and succession of aquatic ecosystems. We investigated the influence of four environmental factors （temperature, salinity, light, and pH） on the allelopathic effects of the macroalgae Corallina pilulifera on red-tide forming Heterosigma akashiwo under laboratory conditions. Each of the factors had four levels： temperature （15, 20, 25, and 30℃）, salinity （10, 20, 30, and 40）, light （20, 100, 200 and 400 gmol/（m2.s））, and pH （5.5, 7, 8.5, and 10. Two-factor experiments were designed for each two environmental factors, with six combination treatments （temperature-salinity, temperature-light, temperature-pH, salinity-light, salinity-pH, and light-pH）. Results showed that the allelopathic effect was significantly influenced by temperature, salinity, light, and pH. As single factors, the low temperature （15 ℃）, low salinity （10）, high-intensity light （400umol/（m2.s））, and high pH （10） treatments substantially enhanced the allelopathic effect. The strongest allelopathic effect of C. pilulifera on H. akashiwo was observed under the following treatments： 15℃ and salinity of 40, 25℃ and pH 10, 25℃ with medium- to high-intensity light at 200-400 umol/（m2.s）, 400umol/（m2.s） and salinity of 10, 400 gmol/（m2·s） and pH 10, and pH 10 with a salinity of 40.
