The presence of feacal-derived pathogens in water is responsible for several infectious diseases and deaths worldwide. As a solution, sources of fecal pollution in waters must be accurately assessed, properly determin...The presence of feacal-derived pathogens in water is responsible for several infectious diseases and deaths worldwide. As a solution, sources of fecal pollution in waters must be accurately assessed, properly determined and strictly controlled. However, the exercise has remained challenging due to the existing overlapping characteristics by different members of faecal coliform bacteria and the inadequacy of information pertaining to the contribution of seasonality and weather condition on tracking the possible sources of pollution. There are continued ef forts to improve the Faecal Contamination Source Tracking(FCST) techniques such as Microbial Source Tracking(MST). This study aimed to make contribution to MST by evaluating the efficacy of combining site specific quantification of faecal contamination indicator bacteria and detection of DNA markers while accounting for seasonality and weather conditions' eff ects in tracking the major sources of faecal contamination in a freshwater system(Donghu Lake, China). The results showed that the use of cyd gene in addition to lacZ and uidA genes differentiates E. coli from other closely related faecal bacteria. The use of selective media increases the pollution source tracking accuracy. BSA addition boosts PCR detection and increases FCST efficiency. Seasonality and weather variability also influence the detection limit for DNA markers.展开更多
Human never stop the journey of exploring the ocean.Since the industry revolution,the advanced technology had been used for drilling platform on sea,submarine cable and subsea tunnel.At the same time,the ocean shippin...Human never stop the journey of exploring the ocean.Since the industry revolution,the advanced technology had been used for drilling platform on sea,submarine cable and subsea tunnel.At the same time,the ocean shipping develops fast in order to pursue the cheaper product materials among countries.But the human activities have greatly influenced the ecology and environment of the ocean."Torres Canyon"oil pollution case,Terrell smeltery arbitration case,Dalian new harbor7.16exploding case all greatly damaged the ocean environment.Therefore,through continual exploration,countries have signed a series of international agreement to construct systematic judging and compensating mechanism.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.31670465,31370504)
文摘The presence of feacal-derived pathogens in water is responsible for several infectious diseases and deaths worldwide. As a solution, sources of fecal pollution in waters must be accurately assessed, properly determined and strictly controlled. However, the exercise has remained challenging due to the existing overlapping characteristics by different members of faecal coliform bacteria and the inadequacy of information pertaining to the contribution of seasonality and weather condition on tracking the possible sources of pollution. There are continued ef forts to improve the Faecal Contamination Source Tracking(FCST) techniques such as Microbial Source Tracking(MST). This study aimed to make contribution to MST by evaluating the efficacy of combining site specific quantification of faecal contamination indicator bacteria and detection of DNA markers while accounting for seasonality and weather conditions' eff ects in tracking the major sources of faecal contamination in a freshwater system(Donghu Lake, China). The results showed that the use of cyd gene in addition to lacZ and uidA genes differentiates E. coli from other closely related faecal bacteria. The use of selective media increases the pollution source tracking accuracy. BSA addition boosts PCR detection and increases FCST efficiency. Seasonality and weather variability also influence the detection limit for DNA markers.
文摘Human never stop the journey of exploring the ocean.Since the industry revolution,the advanced technology had been used for drilling platform on sea,submarine cable and subsea tunnel.At the same time,the ocean shipping develops fast in order to pursue the cheaper product materials among countries.But the human activities have greatly influenced the ecology and environment of the ocean."Torres Canyon"oil pollution case,Terrell smeltery arbitration case,Dalian new harbor7.16exploding case all greatly damaged the ocean environment.Therefore,through continual exploration,countries have signed a series of international agreement to construct systematic judging and compensating mechanism.
