为了解海洋生物修复的发展趋势与研究前沿,本研究以Web of Science核心数据库近17年(1996-2022)的文献为研究对象,运用CiteSpace软件进行文献计量分析与可视化知识图谱分析。研究发现,近年来我国海洋生物修复领域快速发展,发文量位列第...为了解海洋生物修复的发展趋势与研究前沿,本研究以Web of Science核心数据库近17年(1996-2022)的文献为研究对象,运用CiteSpace软件进行文献计量分析与可视化知识图谱分析。研究发现,近年来我国海洋生物修复领域快速发展,发文量位列第一;该领域主要研究内容为海洋污染物、海洋污染治理技术和海洋环境,研究热点集中在“生物降解”和“原油污染生物修复”;国际间的学术合作呈现出“大分散、小集中”的特征,研究机构之间交流较少,跨地区合作亟待加强;当前研究聚焦于海洋生物修复技术的开发与优化,国内外学者应该加强学术交流与合作,突破技术难关,加快恢复海洋生态环境。展开更多
Polycyclic aromatic hydrocarbons （PAHs） in soil retain for a quite long period due to their hydrophobicity and aggregation properties. Biofilm-forming marine bacterial consortium （named as NCPR）, composed of Steno...Polycyclic aromatic hydrocarbons （PAHs） in soil retain for a quite long period due to their hydrophobicity and aggregation properties. Biofilm-forming marine bacterial consortium （named as NCPR）, composed of Stenotrophomonas acidaminiphila NCW702, Alcaligenes faecalis NCW402, Pseudomonas mendocina NR802, Pseudornonas aeruginosa N6P6, and Pseudomonas pseudoalcaligenes NP103, was used for the bioremediation of PAHs in a soil microcosm. Phenanthrene and pyrene were used as reference PAHs. Parameters that can affect PAH degradation, such as chemotaxis, solubility of PAHs in extracellular polymeric substances （EPS）, and catechol 2,3-dioxygenase （C230） activity, were evaluated. P. aeruginosa N6P6 and P. pseudoalcaligenes NP103 showed chemotactic movement towards both the reference PAHs. The solubility of both the PAHs was increased with an increase in EPS concentration （extracted from all the 5 selected isolates）. Significantly （P 〈 0.001） high phenanthrene （70.29%） and pyrene （55.54%） degradation was observed in the bioaugmented soil microcosm. The C230 enzyme activity was significantly （P 〈 0.05） higher in the bioaugmented soil mi- crocosm with phenanthrene added at 173.26 ± 2.06 nmol rain-1 mg-1 protein than with pyrene added at 61.80 ± 2.20 nmol min-1 mg-1 protein. The C230 activity and gas chromatography-mass spectrometer analyses indicated catechol pathway of phenanthrene metabolism. However, the metabolites obtained from the soil microcosm added with pyrene revealed both the catechol and phthalate pathways for pyrene degradation.展开更多
文摘为了解海洋生物修复的发展趋势与研究前沿,本研究以Web of Science核心数据库近17年(1996-2022)的文献为研究对象,运用CiteSpace软件进行文献计量分析与可视化知识图谱分析。研究发现,近年来我国海洋生物修复领域快速发展,发文量位列第一;该领域主要研究内容为海洋污染物、海洋污染治理技术和海洋环境,研究热点集中在“生物降解”和“原油污染生物修复”;国际间的学术合作呈现出“大分散、小集中”的特征,研究机构之间交流较少,跨地区合作亟待加强;当前研究聚焦于海洋生物修复技术的开发与优化,国内外学者应该加强学术交流与合作,突破技术难关,加快恢复海洋生态环境。
基金supported in part by the Department of Biotechnology, Ministry of Science and Technology, Government of India (No. BT/PR14998/GBD/ 27/279/2010)
文摘Polycyclic aromatic hydrocarbons （PAHs） in soil retain for a quite long period due to their hydrophobicity and aggregation properties. Biofilm-forming marine bacterial consortium （named as NCPR）, composed of Stenotrophomonas acidaminiphila NCW702, Alcaligenes faecalis NCW402, Pseudomonas mendocina NR802, Pseudornonas aeruginosa N6P6, and Pseudomonas pseudoalcaligenes NP103, was used for the bioremediation of PAHs in a soil microcosm. Phenanthrene and pyrene were used as reference PAHs. Parameters that can affect PAH degradation, such as chemotaxis, solubility of PAHs in extracellular polymeric substances （EPS）, and catechol 2,3-dioxygenase （C230） activity, were evaluated. P. aeruginosa N6P6 and P. pseudoalcaligenes NP103 showed chemotactic movement towards both the reference PAHs. The solubility of both the PAHs was increased with an increase in EPS concentration （extracted from all the 5 selected isolates）. Significantly （P 〈 0.001） high phenanthrene （70.29%） and pyrene （55.54%） degradation was observed in the bioaugmented soil microcosm. The C230 enzyme activity was significantly （P 〈 0.05） higher in the bioaugmented soil mi- crocosm with phenanthrene added at 173.26 ± 2.06 nmol rain-1 mg-1 protein than with pyrene added at 61.80 ± 2.20 nmol min-1 mg-1 protein. The C230 activity and gas chromatography-mass spectrometer analyses indicated catechol pathway of phenanthrene metabolism. However, the metabolites obtained from the soil microcosm added with pyrene revealed both the catechol and phthalate pathways for pyrene degradation.
