铁在海水中溶解度低,来源有限,是全球40%海域中浮游植物的主要生长限制因子。海洋聚球藻是一种全球分布的超微型蓝藻,也是海洋初级生产力最主要的贡献者之一。受到近岸和大洋环境中铁来源和浓度的影响,海洋聚球藻应对铁限制的适应机制...铁在海水中溶解度低,来源有限,是全球40%海域中浮游植物的主要生长限制因子。海洋聚球藻是一种全球分布的超微型蓝藻,也是海洋初级生产力最主要的贡献者之一。受到近岸和大洋环境中铁来源和浓度的影响,海洋聚球藻应对铁限制的适应机制可能存在差异。本研究基于29株全基因组测序的海洋聚球藻进行了比较基因组学分析。结果表明,海洋聚球藻具有较高的遗传多样性,隶属于GTDB(Genome TaxonomyDatabase)分类法中Cyanobiaceae科的4个属,并且这4个属与原有NCBI(National Center for Biotechnology Information)分类法中海洋聚球藻的亚群分类具有很好的对应关系。功能注释结果表明,近岸和大洋聚球藻的特有基因数量与种类差异较大,近岸株中参与无机离子转运和代谢的特有基因数量大于大洋株。进一步分析海洋聚球藻铁限制相关基因后发现,近岸株在铁的吸收、稳态调节和储存方面的能力强于大洋株,对环境变化的感知和响应能力也更强。本研究重新梳理了海洋聚球藻的进化关系和分类地位,并系统分析了近岸和大洋聚球藻基因组及应对铁限制的适应机制差异,为深入理解海洋聚球藻的环境适应性提供了依据。展开更多
Synechococcus sp.CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation(CA).A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA.The result...Synechococcus sp.CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation(CA).A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA.The results show that Synechococcus sp.CC9311 cells were sensitive to four commonly used antibiotics:ampicillin,kanamycin,spectinomycin,and chloramphenicol.An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV,using a kanamycin resistance gene as selectable marker,was constructed by recombinant polymerase chain reaction.The plasmid was then transformed into Synechococcus sp.CC9311 via electroporation.High transformation efficiency was achieved at a field strength of 2 kV/cm.DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin.In addition,the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.展开更多
文摘铁在海水中溶解度低,来源有限,是全球40%海域中浮游植物的主要生长限制因子。海洋聚球藻是一种全球分布的超微型蓝藻,也是海洋初级生产力最主要的贡献者之一。受到近岸和大洋环境中铁来源和浓度的影响,海洋聚球藻应对铁限制的适应机制可能存在差异。本研究基于29株全基因组测序的海洋聚球藻进行了比较基因组学分析。结果表明,海洋聚球藻具有较高的遗传多样性,隶属于GTDB(Genome TaxonomyDatabase)分类法中Cyanobiaceae科的4个属,并且这4个属与原有NCBI(National Center for Biotechnology Information)分类法中海洋聚球藻的亚群分类具有很好的对应关系。功能注释结果表明,近岸和大洋聚球藻的特有基因数量与种类差异较大,近岸株中参与无机离子转运和代谢的特有基因数量大于大洋株。进一步分析海洋聚球藻铁限制相关基因后发现,近岸株在铁的吸收、稳态调节和储存方面的能力强于大洋株,对环境变化的感知和响应能力也更强。本研究重新梳理了海洋聚球藻的进化关系和分类地位,并系统分析了近岸和大洋聚球藻基因组及应对铁限制的适应机制差异,为深入理解海洋聚球藻的环境适应性提供了依据。
基金Supported by the Key Innovation Project of Institute of Oceanology,Chinese Academy of Sciences(No.2009-2)the Natural Science Foundation of Shandong Province(No.2009ZRB02542)+2 种基金the Foundation of Key Laboratory of Marine Bioactive Substance and Modern Analytical Techniques,SOA(No.MBSMAT-2010-03)the National Natural Science Foundation of China(No.41276164)the Natural Science Foundation of Jiangsu Province(No.BK2012650)
文摘Synechococcus sp.CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation(CA).A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA.The results show that Synechococcus sp.CC9311 cells were sensitive to four commonly used antibiotics:ampicillin,kanamycin,spectinomycin,and chloramphenicol.An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV,using a kanamycin resistance gene as selectable marker,was constructed by recombinant polymerase chain reaction.The plasmid was then transformed into Synechococcus sp.CC9311 via electroporation.High transformation efficiency was achieved at a field strength of 2 kV/cm.DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin.In addition,the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.
