Dimethylsulfide(DMS) and dimethylsulfoniopropionate(DMSP) production by Scrippsiella trochoidea and Prorocentrum minimum was investigated to characterize the effects of physiological stage and salinity on DMS and DMSP...Dimethylsulfide(DMS) and dimethylsulfoniopropionate(DMSP) production by Scrippsiella trochoidea and Prorocentrum minimum was investigated to characterize the effects of physiological stage and salinity on DMS and DMSP pools of these two marine phytoplankton species.Axenic laboratory cultures of the two marine algae were tested for DMSP production and its conversion into DMS.The results demonstrated that both algal species could produce DMS,but the average concentration of DMS per cell in S.trochoidea(12.63 fmol/L) was about six times that in P.minimum(2.01 fmol/L).DMS and DMSP concentrations in algal cultures varied significantly at different growth stages,with high release during the late stationary growth phase and the senescent phase.DMS production induced by three salinities(22,28,34) showed that the DMS concentrations per cell in the two algal cultures increased with increasing salinity,which might result from intra-cellular DMSP up-regulation with the change of osmotic stress.Our study specifies the distinctive contributions of different physiological stages of marine phytoplankton on DMSP and DMS production,and clarifies the influence of salinity conditions on the release of DMS and DMSP.As S.trochoidea and P.minimum are harmful algal bloom species with high DMS production,they might play an additional significant role in the sulfur cycle when a red tide occurs.展开更多
Typical harmful micro-algal species constantly occurred in high density in marine aquaculture ponds in Xiangshan and Sanmen Bay, Zhejiang Province. Fates of the microalgal cells influenced by activity of the cultured ...Typical harmful micro-algal species constantly occurred in high density in marine aquaculture ponds in Xiangshan and Sanmen Bay, Zhejiang Province. Fates of the microalgal cells influenced by activity of the cultured animals largely determined the ef fects of the harmful microalgae. However, it is difficult to detect the in situ process. In this paper, toxic activities of three harmful microalga, namely P rymnesium parvum, Pleurochrysis elongata, Karlodinium veneficum, which were isolated from the local ponds, were comparatively studied based on brine shrimp toxic bioassays. Diff erent lethal activities of live cells, cell debris, cellular extracts, and cell free mediums prepared by different process were analyzed. The results showed that,(1) all of the three microalgal species had density and time dependent lethal ef fects on A rtermia nauplii, while P. parvum was the most toxic one and had acute lethal eff ects in 5 h. No such acute lethal eff ects were observed in P. elongata or K. veneficum;(2) live cells, cell debris and cellular extracts of P. parvum had the same lethal pattern. Prymnesins, toxin from P. parvum, is probably not exotoxic active; For P. elongata, toxic activity mainly came from live cells and cell debris; For K. veneficum, toxic activity was relatively lower compared with the other two species. However, Karlotoxin, toxin from K. veneficum, is exotoxic active. Physical disturbance triggered K. veneficum cells actively releasing toxins, which made it an active predator.展开更多
This article discusses the interaction of aniline and ocean algae based on the standard appraisal method of chemical medicine for algae toxicity. It is showed by experimental results that aniline has pretty toxic effe...This article discusses the interaction of aniline and ocean algae based on the standard appraisal method of chemical medicine for algae toxicity. It is showed by experimental results that aniline has pretty toxic effects on algae. Suspended substances in water can offset some effects of aniline. It also discusses the dynamic constant of first order degradation reaction rate of algae on aniline from the point of view of chemical dynamics.展开更多
Two species of marine phytoplankton, Platymonas helgolandica var. tsingtaoensis and Heterosigma akashiwo, were cul- tivated in bi-algal cultures to investigate the effect of anthracene (ANT) on the interaction betwe...Two species of marine phytoplankton, Platymonas helgolandica var. tsingtaoensis and Heterosigma akashiwo, were cul- tivated in bi-algal cultures to investigate the effect of anthracene (ANT) on the interaction between them. Without ANT, H. akashiwo out-competed P. helgolandica at low initial biomass ratios (P. helgolandica (P): H. akashiwo (H) = 1:4 and 1 : 1), but not at the highest (P:H=4:I). This observation was consistent with the description in Lotka-Volterra two species competition model. It was found that P. helgolandica was excluded at low initial biomass ratios, while the unstable equilibrium between two species was predicted at the highest. For both species, carrying capacity and maximal specific growth rate decreased in bi-algal cultures compared to those in monocultures. H. akashiwo exhibited a higher sensitivity to ANT than P helgolandica. This resulted markedly in a reduced cell den- sity of H. akashiwo but an increased cell density ofP. helgolandica. Carrying capacity ofP. helgolandica was consistently higher in bi-algal cultures with ANT than those without ANT, suggesting that ANT, through the elimination of H. akashiwo, generated the dominance of P helgolandica independently of initial biomass ratios. This study showed a density-dependent effect of harmful alga (H. akashiwo) on dietary alga (P helgolandica), and indicated that ocean pollutant ANT could induce the succession of marine phytoplankton.展开更多
基金Supported by the National Natural Science Foundation of China(Nos. 40525017 and 41030858)the Changjiang Scholars Program,Ministry of Education of China,and the National Basic Research Program of China (973 Program) (No. 2010CB428904)
文摘Dimethylsulfide(DMS) and dimethylsulfoniopropionate(DMSP) production by Scrippsiella trochoidea and Prorocentrum minimum was investigated to characterize the effects of physiological stage and salinity on DMS and DMSP pools of these two marine phytoplankton species.Axenic laboratory cultures of the two marine algae were tested for DMSP production and its conversion into DMS.The results demonstrated that both algal species could produce DMS,but the average concentration of DMS per cell in S.trochoidea(12.63 fmol/L) was about six times that in P.minimum(2.01 fmol/L).DMS and DMSP concentrations in algal cultures varied significantly at different growth stages,with high release during the late stationary growth phase and the senescent phase.DMS production induced by three salinities(22,28,34) showed that the DMS concentrations per cell in the two algal cultures increased with increasing salinity,which might result from intra-cellular DMSP up-regulation with the change of osmotic stress.Our study specifies the distinctive contributions of different physiological stages of marine phytoplankton on DMSP and DMS production,and clarifies the influence of salinity conditions on the release of DMS and DMSP.As S.trochoidea and P.minimum are harmful algal bloom species with high DMS production,they might play an additional significant role in the sulfur cycle when a red tide occurs.
基金Supported by the Earmarked Fund for Modern Agro-industry Technology Research System,China(No.CARS-49)the Ningbo Science and Technology Research Projects,China(No.2017C110003)+2 种基金the Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Development Fundthe National 111 Project of Chinathe K.C.Wong Magna Fund of Ningbo University
文摘Typical harmful micro-algal species constantly occurred in high density in marine aquaculture ponds in Xiangshan and Sanmen Bay, Zhejiang Province. Fates of the microalgal cells influenced by activity of the cultured animals largely determined the ef fects of the harmful microalgae. However, it is difficult to detect the in situ process. In this paper, toxic activities of three harmful microalga, namely P rymnesium parvum, Pleurochrysis elongata, Karlodinium veneficum, which were isolated from the local ponds, were comparatively studied based on brine shrimp toxic bioassays. Diff erent lethal activities of live cells, cell debris, cellular extracts, and cell free mediums prepared by different process were analyzed. The results showed that,(1) all of the three microalgal species had density and time dependent lethal ef fects on A rtermia nauplii, while P. parvum was the most toxic one and had acute lethal eff ects in 5 h. No such acute lethal eff ects were observed in P. elongata or K. veneficum;(2) live cells, cell debris and cellular extracts of P. parvum had the same lethal pattern. Prymnesins, toxin from P. parvum, is probably not exotoxic active; For P. elongata, toxic activity mainly came from live cells and cell debris; For K. veneficum, toxic activity was relatively lower compared with the other two species. However, Karlotoxin, toxin from K. veneficum, is exotoxic active. Physical disturbance triggered K. veneficum cells actively releasing toxins, which made it an active predator.
基金supported by the National Natural Science Foundation of Fujian(B0310001)
文摘This article discusses the interaction of aniline and ocean algae based on the standard appraisal method of chemical medicine for algae toxicity. It is showed by experimental results that aniline has pretty toxic effects on algae. Suspended substances in water can offset some effects of aniline. It also discusses the dynamic constant of first order degradation reaction rate of algae on aniline from the point of view of chemical dynamics.
基金supported by the Ocean Public Welfare Scientific Research Projects, State Oceanic Administration People’s Republic of China (Grant Nos. 200905020, 2010225007, and 201305009)the National Natural Science Foundation of China (Grant No. 31070458)
文摘Two species of marine phytoplankton, Platymonas helgolandica var. tsingtaoensis and Heterosigma akashiwo, were cul- tivated in bi-algal cultures to investigate the effect of anthracene (ANT) on the interaction between them. Without ANT, H. akashiwo out-competed P. helgolandica at low initial biomass ratios (P. helgolandica (P): H. akashiwo (H) = 1:4 and 1 : 1), but not at the highest (P:H=4:I). This observation was consistent with the description in Lotka-Volterra two species competition model. It was found that P. helgolandica was excluded at low initial biomass ratios, while the unstable equilibrium between two species was predicted at the highest. For both species, carrying capacity and maximal specific growth rate decreased in bi-algal cultures compared to those in monocultures. H. akashiwo exhibited a higher sensitivity to ANT than P helgolandica. This resulted markedly in a reduced cell den- sity of H. akashiwo but an increased cell density ofP. helgolandica. Carrying capacity ofP. helgolandica was consistently higher in bi-algal cultures with ANT than those without ANT, suggesting that ANT, through the elimination of H. akashiwo, generated the dominance of P helgolandica independently of initial biomass ratios. This study showed a density-dependent effect of harmful alga (H. akashiwo) on dietary alga (P helgolandica), and indicated that ocean pollutant ANT could induce the succession of marine phytoplankton.