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一种海洋领鞭毛虫的分离与鉴定
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作者 贾晓燕 熊源 +4 位作者 黄凌风 林施泉 陆家昌 吴林南 李玲 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2015年第3期436-439,共4页
采用微液滴操作法在厦门海域分离培养了一株海洋鞭毛虫C6,运用显微镜观察和18SrDNA基因技术鉴定其种类,并将其与GenBank中已知的14种海洋鞭毛虫18SrDNA序列比较,用NJ法和UPGMA法构建系统发育树.通过PCR扩增获得1 774bp 18SrDNA序列,与N... 采用微液滴操作法在厦门海域分离培养了一株海洋鞭毛虫C6,运用显微镜观察和18SrDNA基因技术鉴定其种类,并将其与GenBank中已知的14种海洋鞭毛虫18SrDNA序列比较,用NJ法和UPGMA法构建系统发育树.通过PCR扩增获得1 774bp 18SrDNA序列,与NCBI已登陆的其他同源序列进行系统分析,结果显示此海洋鞭毛虫属于领鞭毛虫(choanoflagellates).与已知种Monosiga brevicollis的相似性为99%,为进一步研究该种鞭毛虫提供了遗传背景,但要明确其种类还需其他研究佐证. 展开更多
关键词 海洋鞭毛虫 分离 18SrDNA 分子鉴定
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Competition Among Dinoflagellate Alexandrium Tamarense, Raphidophyte Heterosigma Carterae and Diatom Skeletonema Costatum under Combinations of Two Temperatures and Five Salinities 被引量:2
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作者 颜天 周名江 钱培元 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2003年第3期245-250,共6页
Competition among HAB (Harmful Algal Bloom) species Dinoflagellate Alexandrium tamarense , Raphidophyte Heterosigma carterae , and Diatom Skeletonema costatum was studied in the laboratory. Experiments with these thre... Competition among HAB (Harmful Algal Bloom) species Dinoflagellate Alexandrium tamarense , Raphidophyte Heterosigma carterae , and Diatom Skeletonema costatum was studied in the laboratory. Experiments with these three major HAB species under combinations of different salinities (10, 18, 25, 30, 35) and temperatures (19℃, 25℃) were carried out. The results showed that S. costatum successfully competed with the other two species at salinities of 18, 25, 30, and 35 at temperatures of 19℃ and 25℃. However, H. carterae showed its advantage at low salinity of 10 and became the single dominant species at salinity 10 and 25℃. A. tamarense could not compete successfully with the other two species especially at low salinities. However, it could remain at low density in the presence of higher densities of other algae. 展开更多
关键词 Alexandrium tamarense Heterosigma carterae Skeletonema costatum Harmful Algal Bloom competition temperature SALINITY
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