Great advances have been achieved in abstinence treatment on heroin-dependence and prevention of drug-reusing, and a variety of traditional Chinese medicine (TCM) drugs or preparations have been developed and applied ...Great advances have been achieved in abstinence treatment on heroin-dependence and prevention of drug-reusing, and a variety of traditional Chinese medicine (TCM) drugs or preparations have been developed and applied in clinic in recent years. The Zheng Tong Ning Granules (正通宁颗粒冲剂), for example, has been tested on healthy volunteers and proved to be more tolerable than clonidine1. However, they are designed only for certain symptoms appeared in a certain stage of the abstinence treatment. A comprehensive study on TCM syndrome types of heroin-dependence and the relevant treatment are still lacking. Following is our pioneer attempt made in this field.展开更多
Objective： To observe the effects of electroacupuncture on withdrawal anxiety in heroin-dependent patients. Methods： Sixty heroin-withdrawal subjects with negative urine morphine were digitally randomized into two g...Objective： To observe the effects of electroacupuncture on withdrawal anxiety in heroin-dependent patients. Methods： Sixty heroin-withdrawal subjects with negative urine morphine were digitally randomized into two groups： acupuncture group in which 30 cases were treated by electroacupuncture and control group in which 30 cases were given no treatment. The patients were assessed by Hamilton anxiety scale before treatment and one week, two weeks and three weeks after treatment. Results： After treatment, the anxiety symptoms of heroin-dependent patients were improved more significantly in the acupuncture group than in the control group （P〈0.01）. Conclusion： Electroacupuncture has good effects on anxiety symptoms and emotions of heroin-dependent patients.展开更多
Obj ective： To observe and analyze the intervention effects of needling different points for post- withdrawal syndrome of heroin dependence, and thus provide clinical basis for screening specific points. Methods： A ...Obj ective： To observe and analyze the intervention effects of needling different points for post- withdrawal syndrome of heroin dependence, and thus provide clinical basis for screening specific points. Methods： A total of 60 males with heroin dependence were randomly allocated into 3 groups, the Neiguan （PC 6） group, the Shenmen （HT 7） group and the control group. The results were observed and evaluated before, during, and 3 weeks after treatment using the rating scale for post-withdrawal syndrome and Hamilton anxiety scale （HAMA）. Results： In regards to the control of post-withdrawal symptoms, the groups of Neiguan （PC 6） and Shenmen （HT 7） showed statistical difference when compared with the control group （P〈0.05, P〈0.01）; however, there was no statistical difference between the Neiguan （PC 6） group and Shenmen （HT 7） group （P〉0.05）. Conclusion： Acupuncture can alleviate the post-withdrawal syndrome and anxiety of heroin dependence. Although both Neiguan （PC 6） and Shenmen （HT 7） have equally remarkable effects, Shenmen （HT 7） have a better tendency for marked effect for heart-mind related symptoms.展开更多
Objective：To observe the effect of acupuncture in regulating ubiquitin-proteasome pathway （UPP）,and discuss the action of acupuncture in intervening heroin-induced brain damage.Methods：Thirty male Sprague-Dawley ...Objective：To observe the effect of acupuncture in regulating ubiquitin-proteasome pathway （UPP）,and discuss the action of acupuncture in intervening heroin-induced brain damage.Methods：Thirty male Sprague-Dawley （SD） rats were divided into a control group,a model group and an acupuncture group by using the random number table.Rats in the model and acupuncture groups received intramuscular heroin injection for successive 8 d at a progressively increased dose.Afterwards,the injection was suspended for 5 d for withdrawal.The heroin relapse rat model was established by repeating the drug addiction and withdrawal process for 3 times.The control group followed the step of the model establishment,but was given intramuscular injection of normal saline at the stage of addiction and no intervention at the stage of withdrawal;the model group was given intramuscular heroin injection at a progressively increased dose at the addiction stage and no intervention at the withdrawal stage;the acupuncture group was dealt in the same way as the model group at the addiction stage,but received acupuncture at Baihui （GV 20） and Dazhui （GV 14） at the withdrawal stage,with the needles retained for 30 min each time,1 session a day,for successive 5 d.On the 39th day,brain tissues were extracted from the hippocampus and ventral tegmental area （VTA） of the three groups of rats.The apoptosis of brain nerve cells was detected by using terminal deoxynucleotidyl transferase-mediated nick and labeling （TUNEL）.The mRNA and protein expressions of ubiquitin （Ub）,ubiquitin protein ligase （E3） and 26S were examined by immunohistochemistry and quantitative real-time polymerase chain reaction （RT-qPCR）.Results：Compared with the model group,rat＇s hippocampus and VTA in the acupuncture group showed significantly fewer cells positively stained by TUNEL staining （P〈0.01）,and its mRNA and protein expressions of Ub,E3,26S were significantly lower （P〈0.01）.Conclusion：Reducing nerve cell apoptosis and regulating the mRNA and protein expressions of Ub,E3 and 26S in rat＇s hippocampus and VTA are possibly one of the action mechanisms of acupuncture in intervening heroin-induced brain damage.展开更多
Objective： To observe the influence of acupuncture on sleep disorders and anxiety in patients with heroin dependence. Methods： Sixty-two male patients with heroin dependence were divided into two groups by random nu...Objective： To observe the influence of acupuncture on sleep disorders and anxiety in patients with heroin dependence. Methods： Sixty-two male patients with heroin dependence were divided into two groups by random number table. Thirty-five cases in the acupuncture group were treated by puncturing Baihui （GV 20）, Neiguan （PC 6）, and Shenmen （HT 7） and moxibustion at Zusanli （ST 36）. Twenty-seven cases in the control group did not receive any therapeutic measures. The therapeutic effects were summarized after observation of 8 weeks. Results： After treatment, the global scores and standard scores of anxiety declined in comparison with those before the treatment in both two groups, but there was statistical difference only in the acupuncture group （P〈0.05）. There were no statistical differences in the global score and the standard score of anxiety between the two groups, but the standard scores in the acupuncture group reached the normal level. After treatment, only the sleep latency was obviously improved in the control group （P〈0.05）. The syndromes of sleep disorder were improved noticeable in the acupuncture group （P〈0.05）, and the actual sleep time was improved better than those in the control group （P〈0.05）, and PSQI global scores decreased below the normal level. Conclusion： Acupuncture has the effects to improve sleep disorders and anxious emotion in the patients with heroin dependence.展开更多
Objective： This present study is to establish a rat model of intravenous heroin self-administration and observe the effect of electro-acupuncture on the heroin seeking behavior of the heroin self-administrating rat. ...Objective： This present study is to establish a rat model of intravenous heroin self-administration and observe the effect of electro-acupuncture on the heroin seeking behavior of the heroin self-administrating rat. Methods： Six Male Wistar rats were surgically implanted with an intra jugular intravenous catheters under sodium pentobarbital anesthesia, following a minimum recovery of 7 days, the rats were trained by nose-poke response with one daily 4-hour session for 14 consecutive days to self administer heroin （50 μg /kg / per injection）, the session ended after 25 infusions were earned or 4 hours had passed, whichever came first, the reinforcement schedule was fixed ratio 1 （FR1） that the rat received a heroin injection for each nose poke response. And then the rats were given electro-acupuncture treatment daily 30min prior to each session and then were trained with one daily 4-h session for 7 consecutive days. Results： Stable intravenous heroin self-administration behavior of rats was established after 7-8 days＇ drug training, electro-acupuncture given to the stable heroin self-administrating rat 30 min prior to each session could reduce the drug self-administration behavior, namely the total heroin intake in each session was reduced, especially the 1^th hour heroin intake, the initiation of first nose-poke response in each session was relayed, and the haste heroin-seeking behavior also became slower. Conclusions： The above results suggested that the electro-acupuncture had better detoxification effect.展开更多
文摘Great advances have been achieved in abstinence treatment on heroin-dependence and prevention of drug-reusing, and a variety of traditional Chinese medicine (TCM) drugs or preparations have been developed and applied in clinic in recent years. The Zheng Tong Ning Granules (正通宁颗粒冲剂), for example, has been tested on healthy volunteers and proved to be more tolerable than clonidine1. However, they are designed only for certain symptoms appeared in a certain stage of the abstinence treatment. A comprehensive study on TCM syndrome types of heroin-dependence and the relevant treatment are still lacking. Following is our pioneer attempt made in this field.
基金Shanghai Municipal Education Commission(CZ0523)Shanghai Leading Academic Discipline Project(T0302)
文摘Objective： To observe the effects of electroacupuncture on withdrawal anxiety in heroin-dependent patients. Methods： Sixty heroin-withdrawal subjects with negative urine morphine were digitally randomized into two groups： acupuncture group in which 30 cases were treated by electroacupuncture and control group in which 30 cases were given no treatment. The patients were assessed by Hamilton anxiety scale before treatment and one week, two weeks and three weeks after treatment. Results： After treatment, the anxiety symptoms of heroin-dependent patients were improved more significantly in the acupuncture group than in the control group （P〈0.01）. Conclusion： Electroacupuncture has good effects on anxiety symptoms and emotions of heroin-dependent patients.
基金supported by Shanghai Leading Academic Discipline Project(S30304)Youth Foundation of Shanghai Municipal Health Bureau(2008Y124)Innovation Project of Shanghai Municipal Education Commission(12YZ070)
文摘Obj ective： To observe and analyze the intervention effects of needling different points for post- withdrawal syndrome of heroin dependence, and thus provide clinical basis for screening specific points. Methods： A total of 60 males with heroin dependence were randomly allocated into 3 groups, the Neiguan （PC 6） group, the Shenmen （HT 7） group and the control group. The results were observed and evaluated before, during, and 3 weeks after treatment using the rating scale for post-withdrawal syndrome and Hamilton anxiety scale （HAMA）. Results： In regards to the control of post-withdrawal symptoms, the groups of Neiguan （PC 6） and Shenmen （HT 7） showed statistical difference when compared with the control group （P〈0.05, P〈0.01）; however, there was no statistical difference between the Neiguan （PC 6） group and Shenmen （HT 7） group （P〉0.05）. Conclusion： Acupuncture can alleviate the post-withdrawal syndrome and anxiety of heroin dependence. Although both Neiguan （PC 6） and Shenmen （HT 7） have equally remarkable effects, Shenmen （HT 7） have a better tendency for marked effect for heart-mind related symptoms.
文摘Objective：To observe the effect of acupuncture in regulating ubiquitin-proteasome pathway （UPP）,and discuss the action of acupuncture in intervening heroin-induced brain damage.Methods：Thirty male Sprague-Dawley （SD） rats were divided into a control group,a model group and an acupuncture group by using the random number table.Rats in the model and acupuncture groups received intramuscular heroin injection for successive 8 d at a progressively increased dose.Afterwards,the injection was suspended for 5 d for withdrawal.The heroin relapse rat model was established by repeating the drug addiction and withdrawal process for 3 times.The control group followed the step of the model establishment,but was given intramuscular injection of normal saline at the stage of addiction and no intervention at the stage of withdrawal;the model group was given intramuscular heroin injection at a progressively increased dose at the addiction stage and no intervention at the withdrawal stage;the acupuncture group was dealt in the same way as the model group at the addiction stage,but received acupuncture at Baihui （GV 20） and Dazhui （GV 14） at the withdrawal stage,with the needles retained for 30 min each time,1 session a day,for successive 5 d.On the 39th day,brain tissues were extracted from the hippocampus and ventral tegmental area （VTA） of the three groups of rats.The apoptosis of brain nerve cells was detected by using terminal deoxynucleotidyl transferase-mediated nick and labeling （TUNEL）.The mRNA and protein expressions of ubiquitin （Ub）,ubiquitin protein ligase （E3） and 26S were examined by immunohistochemistry and quantitative real-time polymerase chain reaction （RT-qPCR）.Results：Compared with the model group,rat＇s hippocampus and VTA in the acupuncture group showed significantly fewer cells positively stained by TUNEL staining （P〈0.01）,and its mRNA and protein expressions of Ub,E3,26S were significantly lower （P〈0.01）.Conclusion：Reducing nerve cell apoptosis and regulating the mRNA and protein expressions of Ub,E3 and 26S in rat＇s hippocampus and VTA are possibly one of the action mechanisms of acupuncture in intervening heroin-induced brain damage.
基金supported by Anhui Provincial Programs for Science and Technology Development(07010302205) and Provincial Key Lab of Acupuncture Basis and Technology
文摘Objective： To observe the influence of acupuncture on sleep disorders and anxiety in patients with heroin dependence. Methods： Sixty-two male patients with heroin dependence were divided into two groups by random number table. Thirty-five cases in the acupuncture group were treated by puncturing Baihui （GV 20）, Neiguan （PC 6）, and Shenmen （HT 7） and moxibustion at Zusanli （ST 36）. Twenty-seven cases in the control group did not receive any therapeutic measures. The therapeutic effects were summarized after observation of 8 weeks. Results： After treatment, the global scores and standard scores of anxiety declined in comparison with those before the treatment in both two groups, but there was statistical difference only in the acupuncture group （P〈0.05）. There were no statistical differences in the global score and the standard score of anxiety between the two groups, but the standard scores in the acupuncture group reached the normal level. After treatment, only the sleep latency was obviously improved in the control group （P〈0.05）. The syndromes of sleep disorder were improved noticeable in the acupuncture group （P〈0.05）, and the actual sleep time was improved better than those in the control group （P〈0.05）, and PSQI global scores decreased below the normal level. Conclusion： Acupuncture has the effects to improve sleep disorders and anxious emotion in the patients with heroin dependence.
文摘Objective： This present study is to establish a rat model of intravenous heroin self-administration and observe the effect of electro-acupuncture on the heroin seeking behavior of the heroin self-administrating rat. Methods： Six Male Wistar rats were surgically implanted with an intra jugular intravenous catheters under sodium pentobarbital anesthesia, following a minimum recovery of 7 days, the rats were trained by nose-poke response with one daily 4-hour session for 14 consecutive days to self administer heroin （50 μg /kg / per injection）, the session ended after 25 infusions were earned or 4 hours had passed, whichever came first, the reinforcement schedule was fixed ratio 1 （FR1） that the rat received a heroin injection for each nose poke response. And then the rats were given electro-acupuncture treatment daily 30min prior to each session and then were trained with one daily 4-h session for 7 consecutive days. Results： Stable intravenous heroin self-administration behavior of rats was established after 7-8 days＇ drug training, electro-acupuncture given to the stable heroin self-administrating rat 30 min prior to each session could reduce the drug self-administration behavior, namely the total heroin intake in each session was reduced, especially the 1^th hour heroin intake, the initiation of first nose-poke response in each session was relayed, and the haste heroin-seeking behavior also became slower. Conclusions： The above results suggested that the electro-acupuncture had better detoxification effect.
