目的:探讨海红果多糖(polysaccharide from Malus prunifolia Borkh,PFM)提取工艺条件及其体外抗氧化活性。方法:采用三因素三水平正交试验设计,考察提取温度、时间和料液比3个因素对海红果多糖提取工艺的影响;以VC为对照,应用DPPH法测...目的:探讨海红果多糖(polysaccharide from Malus prunifolia Borkh,PFM)提取工艺条件及其体外抗氧化活性。方法:采用三因素三水平正交试验设计,考察提取温度、时间和料液比3个因素对海红果多糖提取工艺的影响;以VC为对照,应用DPPH法测定海红果多糖清除有机自由基能力、ABTS法测定其总抗氧化能力、Fenton反应测定其清除羟自由基能力。结果:PFM最佳提取工艺条件为提取温度90℃、提取时间6h、料液比1:10(g/mL),此条件下PFM得率为6.75%;PFM对羟自由基具有较强的清除作用(P<0.05),效果优于清除DPPH自由基和ABTS+自由基,但弱于VC。结论:优化海红果提取工艺得到的PFM具有较强的体外抗氧化活性。展开更多
Factors influencing the stability of the red pigment extracting form Haihongguo fruit,a natural fruit,were investigated in this paper.The results showed that the pigment was stable in acid,under the light and heat con...Factors influencing the stability of the red pigment extracting form Haihongguo fruit,a natural fruit,were investigated in this paper.The results showed that the pigment was stable in acid,under the light and heat condition.The food additions such as starch,carbohydrate,ascorbie acid,sucrose,glucose sodium benzoate ect.,have no bad influence on the color quality of the pigment,so did the reace element for human body such as K+,Na+,Ca2+,Zn2+,ect.,However the Fe3+,Sn2+,H2O and Na2SO3,have redox reaction with the pigment and change its color.The exaction of red pigment in the Haihongguo Fruit may provide the new sources for the food addition.展开更多
文摘目的:探讨海红果多糖(polysaccharide from Malus prunifolia Borkh,PFM)提取工艺条件及其体外抗氧化活性。方法:采用三因素三水平正交试验设计,考察提取温度、时间和料液比3个因素对海红果多糖提取工艺的影响;以VC为对照,应用DPPH法测定海红果多糖清除有机自由基能力、ABTS法测定其总抗氧化能力、Fenton反应测定其清除羟自由基能力。结果:PFM最佳提取工艺条件为提取温度90℃、提取时间6h、料液比1:10(g/mL),此条件下PFM得率为6.75%;PFM对羟自由基具有较强的清除作用(P<0.05),效果优于清除DPPH自由基和ABTS+自由基,但弱于VC。结论:优化海红果提取工艺得到的PFM具有较强的体外抗氧化活性。
文摘Factors influencing the stability of the red pigment extracting form Haihongguo fruit,a natural fruit,were investigated in this paper.The results showed that the pigment was stable in acid,under the light and heat condition.The food additions such as starch,carbohydrate,ascorbie acid,sucrose,glucose sodium benzoate ect.,have no bad influence on the color quality of the pigment,so did the reace element for human body such as K+,Na+,Ca2+,Zn2+,ect.,However the Fe3+,Sn2+,H2O and Na2SO3,have redox reaction with the pigment and change its color.The exaction of red pigment in the Haihongguo Fruit may provide the new sources for the food addition.