Creutzfeldt-Jakob病一例

患者，女，59岁，农民，以进行性痴呆、言语不利、行走不稳4个月为主诉入院。

超声处理对羊瘙痒病脑组织中PrP^(Sc)聚集状态的影响

目的 研究超声处理对感染羊瘙痒症仓鼠脑组织中PrPSc聚集状态的影响 ,寻找产生PrPSc低聚体的条件。方法 裂解液制备脑组织提取物 ,用各种超声条件处理不同阶段的脑组织提取物 ;以蛋白酶消化后的Westernblot方法和图象分析系统观测PrPSc蛋白的分布和聚集状态。结果 适当的超声处理 (15s共 30次 )可增加脑组织匀浆上清中PrPSc含量 1 2 9～ 1 5 8倍 ;同样条件下超声处理可明显增加羊瘙痒因子 2 6 3K感染仓鼠脑组织匀浆上清中PrP蛋白总量 ,而对正常对照仓鼠脑组织匀浆上清中PrP蛋白总量影响不大 ;对经常规高速离心获得的PrPSc的超声处理显示约 90 %的PrPSc存在于离心上清液中。结论 对感染动物脑组织进行超声处理可增加PrPSc 的提取量 ,利于实验室检测。适当的超声处理可破碎大的相对分子质量的PrPSc聚集物 ,产生小的相对分子质量的PrPSc产物。

Characteristics of spongiform leukoencephalopathy induced by heroin: MRI detection

Objective To elucidate the imaging characteristics of spongiform leukoencephalopathy induced by heroin and the diagnostic value of MRI examination.Methods Eleven cases of heroin addiction were examined by MRI.The sequences included T1-weighted imaging(T1WI),T2-weighted imaging(T2WI)and fast fluid-attenuated inversion recovery pulse sequence(Flair).Results Bilateral symmetrical lesions were located in the white matter of the cerebrum and cerebellum,the internal capsule in all cases,and the callosum in 6 cases.Elliptical lesions in the mesencephalon were found in two cases.These lesions were shown as hypointense signals on T1WI and hyperintense signals on T2WI and Flair's imaging.Cerebellar white matter suffered more severely,but the dentate nuclei were not invaded.Conclusion Heroin-induced spongiform leukoencephalopathy has characteristic patterns in MRI,which are very specific to this form of the disease.