用高灵敏度分光光度法测定海豚肝脏中的微量硒

A highly sensitive spectrophotometric method for determination of selenium(IV) has been developed.It has been based on Se(IV) oxidation of I - in a weak acid medium and formation of the 1∶1 ion associatin complex of I - with Butylrhodamine B in the presence of poly(vinyl alcohol).The wavelength of maximum absorbance of this complex was at 590nm,The molar absorptivity was 2.57 ×10 5L.mol -1 ·cm -1 and Beer’s law was obeyed in the concentration range of 40～500ng/mL.The detection limit was 40ng/mL.Interfering ions were eliminated by using thiohydroxy cotton so that the selectivity of this method was good.The color system was used to determine the microamounts of Se in the liver of dophine and huangqi.Satisfactory results were obtained.

微波消化冷原子吸收法测定海豚肝脏中的汞

采用微波消化样品后用冷原子吸收法测定海豚肝脏中的汞 ,仅用 1:1HNO3 消化样品 ,有良好的汞溶出率和回收率 ,方法简便、快速。检出限 1.6 5ng ,线性范围 0～ 5 0 μg/L ,相对标准偏差 4.0 9% ,测定回收率 98.8%～ 10 0 .4%。用该方法测定四个海豚肝脏粉末样中汞含量分别为 6 35 .5、6 77.1、6 72 .8、6 0 3.3μg/g。