Glutathione S-transferases (GSTs) are phase II enzymes that facilitate the detoxification of xenobioties and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis ph...Glutathione S-transferases (GSTs) are phase II enzymes that facilitate the detoxification of xenobioties and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis philippinarum GSTs (VpGSTs) and four Mytilus galloprovincialis GSTs (MgGSTs) following exposure to BDE-47. Differential expressions of the seven VpGSTs and four MgGSTs transcripts were observed, with differences between the hepatopancreas and gills. Among these GSTs, the sigma classes (VpGSTS1, VpGSTS2, VpGSTS3, MgGST1, and MgGST3) were highly expressed in response to BDE-47 exposure, demonstrating their potential as molecular biomarkers for environmental biomonitoring studies. We obtained the three-dimensional crystal structures of VpGSTs and MgGSTs by homologous modeling. A model to elucidate the binding interactions between the ligands and receptors was defined by molecular docking, Hydrophobic and n were the most often observed interactions between BDE-47 and the GSTs.展开更多
Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture (IMTA) systems. A beneficial relationship between seaweed and bivalve in the seaweed- based IMTA system has b...Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture (IMTA) systems. A beneficial relationship between seaweed and bivalve in the seaweed- based IMTA system has been confirmed, but the trophic importance of seaweed-derived particulate organic materials to the co-cultured bivalve is still unclear. We evaluated the trophic importance of the kelp Saccharinajaponica to the co-cultured scallop Chlamysfarreri in a typical IMTA farm in Sungo Bay (Weihai, North China). The dynamics of detritus carbon in the water were monitored during the culturing period. The proportion of kelp-derived organic matter in the diet of the co-cultured scallop was assessed via the stable carbon isotope method. Results showed that the detritus carbon in the water ranged from 75.52 to 265.19 ~tg/L, which was 25.6% to 73.8% of total particulate organic carbon (TPOC) during the study period. The amount of detritus carbon and its proportion in the TPOC changed throughout the culture cycle of the kelp. Stable carbon isotope analysis showed that the cultured scallop obtained 14.1% to 42.8% of its tissue carbon from the kelp, and that the percentages were closely correlated with the proportion of detritus carbon in the water (F=0.993, P=0.003). Evaluation showed that for 17 000 tons (wet weight) of annual scallop production, the kelp contributed about 139.3 tons of carbon (535.8 tons of dry mass). This confirms that cultured kelp plays a similar trophic role in IMTA systems as it does in a natural kelp bed. It is a major contributor to the detritus pool and supplies a vital food source to filter-feeding scallops in the IMTA system, especially during winter and early spring when phytoplankton are scarce.展开更多
For the supply of fish and shellfish to consumers in fresh condition, clean handling after catch from the sea is essential. According to HACCP (Hazard Analysis and Critical Control Points), it is important to meet s...For the supply of fish and shellfish to consumers in fresh condition, clean handling after catch from the sea is essential. According to HACCP (Hazard Analysis and Critical Control Points), it is important to meet such requirement by keeping fish and shellfish under a certain low temperature and clean conditions after catching. The deep ocean water (DOW) characterized by low temperature and cleanliness has been chosen for fish and shellfish handlings, particularly for salmon, cod, and sea urchin in Town 'Rausu' in Hokkaido, Japan. DOW below 2.9℃ of an amount of nearly 5 000 m^3 is planned to be pumped up every day from a depth of about 350 m, and temporarily stored in a large simulated tank on land. DOW is then supplied to fish boats through hydrants distributed throughout the harbor and used for keeping salmon in clean and cold conditions. Ice made from DOW is also used for lowering temperature if necessary. DOW and ice made from DOW are also used during the transportation of fish and shellfish. The entire system is scheduled to be completed by the summer of 2005.展开更多
A 12-month program of monitoring potentially toxic microalgae(that produce lipophilic shellfi sh toxins; LSTs) and their toxins in bivalves was conducted from April 2006 to March 2007 in the Nanji Islands, East China ...A 12-month program of monitoring potentially toxic microalgae(that produce lipophilic shellfi sh toxins; LSTs) and their toxins in bivalves was conducted from April 2006 to March 2007 in the Nanji Islands, East China Sea. Two Dinophysis species, D. caudata and D. acuminata, were identifi ed, and D. caudat a was found to be the dominant species. D. caudata was detected in water samples between April and June 2006, and between February and March 2007. It reached its highest abundances in May, with a mean abundance of 1.38×10 2 cells/L in surface water and 1.25×10 2 cells/L in bottom water(<10 m deep). The temporal distribution of D. caudata was associated with the occurrence of LSTs in bivalve samples, which mostly occurred at the same time as D. caudata blooms, between April and July 2006. All of the cultured bivalves sampled between April and June were contaminated with LSTs, with an average toxicity of 85 μg okadaic acid(OA) eq./100 g meat, which was four times higher than the Chinese regulatory limit(20 μg OA eq./100 g meat). Ten out of fi fteen wild samples(66.7%) collected during the same period were positive for LSTs, and contained an average LST toxicity of 45 μg OA eq./100 g meat(more than twice the regulatory value). Cultured Patinopecten yessoensis collected on 15 May 2006 had the highest toxicity, 320 μg OA eq./100 g meat, and relatively high toxicities(80 to 160 μg OA eq./100 g meat) were found in bivalves until the end of July.展开更多
It has been classed 856 species of the mollusks which belong to 120 families and five classes living in western coast of the Tonkin Gulf. The class Gastropod was the most abundant, with 449 species, the Bivalve with 3...It has been classed 856 species of the mollusks which belong to 120 families and five classes living in western coast of the Tonkin Gulf. The class Gastropod was the most abundant, with 449 species, the Bivalve with 368 species, the Scaphopoda with 10 species, the Cephalopods with 19 species and the Amphineura with eight species. Standing stock was estimated about 366,749 tons, that annually caught 80,000 tons. The causes of the resource depletion were human and natural, but the human factors played a key role. The fundamental orientations for developing the resources include: exploiting during harvest seasons, planning suitable aquaculture, moderate exploitation so the resources can restore themselves, combining exploitation and conservation.展开更多
Marine invertebrates and fish are well known for their remarkable genetic diversity, which is commonly explained by large population size and the characteristic dispersive nature of their early, planktonic life histor...Marine invertebrates and fish are well known for their remarkable genetic diversity, which is commonly explained by large population size and the characteristic dispersive nature of their early, planktonic life history. Other potential sources of diversity in marine animals, such as a higher mutation rate, have been much less considered, though evidence for a high genetic load in marine bivalves has been accumulating for nearly half a century. In this review, I examine evidence for a higher genetic load in marine animals from studies of molecular marker segregation and linkage over the last 40 years, and survey recent work examining mutational load with molecular evolution approaches. Overall, marine animals appear to have higher genetic load than terrestrial animals (higher dn/ds ratios, inbreeding load, and segregation dis'tortion), though results are mixed for marine fish and data are lacking for many marine animal groups. Bivalves (oysters) have the highest loads observed among marine animals, comparable only to long-lived plants; however, more data is needed from other bivalves and more marine invertebrate taxa generally. For oysters, a higher load may be related to a chronically lower effective population size that, in concert with a higher mutational rate, elevate the number of deleterious mutations observed. I suggest that future studies use high-throughput sequencing approaches to examine (1) polymorphism in genomescale datasets across a wider range of marine animals at the population level and (2) intergenerational mutational changes between parents and offspring in crosses of aquaculture species to quantify mutation rates.展开更多
The Beiqian site,located in Jimo city,Qingdao,went through three archaeological excavations,and a large number of human and animal bones in the Dawenkou culture and the Zhou Dynasty period were unearthed.By carbon and...The Beiqian site,located in Jimo city,Qingdao,went through three archaeological excavations,and a large number of human and animal bones in the Dawenkou culture and the Zhou Dynasty period were unearthed.By carbon and nitrogen stable isotope analysis,the diet of ancestors is expected to be recovered,and the domesticated plants and animals will be discussed.The investigated results showed that the food structure of ancestors in both periods included food crops,mainly C4 foods,and meat,mainly marine shellfish and livestock.For domestic pigs,they mainly eat C3 plants,and were affected by the lifestyle of ancestors.This study also showed that the diet of ancestors is the shellfish rather than marine fish despite the Beiqian site is located near the shoreline.We can conclude that the diet of ancestors in Dawenkou culture period included millet crops(C4plants),shellfish,marine food,and livestock,representing the lifestyle of farming,domestication,and fishing,supplemented by hunting.In the Zhou dynasty period the meat resource was enriched by domesticated animals and marine fisheries,and the living conditions were significantly improved.展开更多
基金Supported by the National Natural Science Foundation of China(No.21107136)the International Foundation for Science(No.F/5230-1)
文摘Glutathione S-transferases (GSTs) are phase II enzymes that facilitate the detoxification of xenobioties and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis philippinarum GSTs (VpGSTs) and four Mytilus galloprovincialis GSTs (MgGSTs) following exposure to BDE-47. Differential expressions of the seven VpGSTs and four MgGSTs transcripts were observed, with differences between the hepatopancreas and gills. Among these GSTs, the sigma classes (VpGSTS1, VpGSTS2, VpGSTS3, MgGST1, and MgGST3) were highly expressed in response to BDE-47 exposure, demonstrating their potential as molecular biomarkers for environmental biomonitoring studies. We obtained the three-dimensional crystal structures of VpGSTs and MgGSTs by homologous modeling. A model to elucidate the binding interactions between the ligands and receptors was defined by molecular docking, Hydrophobic and n were the most often observed interactions between BDE-47 and the GSTs.
基金Supported by the National Key Technologies R&D Program of China(No.2011BAD13B02)the NSFC-Shandong Joint Fund for Marine Science Research Centers(No.U1406403)the Marine Strategic Priority Research Program of Chinese Academy of Sciences(No.XDA11020704)
文摘Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture (IMTA) systems. A beneficial relationship between seaweed and bivalve in the seaweed- based IMTA system has been confirmed, but the trophic importance of seaweed-derived particulate organic materials to the co-cultured bivalve is still unclear. We evaluated the trophic importance of the kelp Saccharinajaponica to the co-cultured scallop Chlamysfarreri in a typical IMTA farm in Sungo Bay (Weihai, North China). The dynamics of detritus carbon in the water were monitored during the culturing period. The proportion of kelp-derived organic matter in the diet of the co-cultured scallop was assessed via the stable carbon isotope method. Results showed that the detritus carbon in the water ranged from 75.52 to 265.19 ~tg/L, which was 25.6% to 73.8% of total particulate organic carbon (TPOC) during the study period. The amount of detritus carbon and its proportion in the TPOC changed throughout the culture cycle of the kelp. Stable carbon isotope analysis showed that the cultured scallop obtained 14.1% to 42.8% of its tissue carbon from the kelp, and that the percentages were closely correlated with the proportion of detritus carbon in the water (F=0.993, P=0.003). Evaluation showed that for 17 000 tons (wet weight) of annual scallop production, the kelp contributed about 139.3 tons of carbon (535.8 tons of dry mass). This confirms that cultured kelp plays a similar trophic role in IMTA systems as it does in a natural kelp bed. It is a major contributor to the detritus pool and supplies a vital food source to filter-feeding scallops in the IMTA system, especially during winter and early spring when phytoplankton are scarce.
文摘For the supply of fish and shellfish to consumers in fresh condition, clean handling after catch from the sea is essential. According to HACCP (Hazard Analysis and Critical Control Points), it is important to meet such requirement by keeping fish and shellfish under a certain low temperature and clean conditions after catching. The deep ocean water (DOW) characterized by low temperature and cleanliness has been chosen for fish and shellfish handlings, particularly for salmon, cod, and sea urchin in Town 'Rausu' in Hokkaido, Japan. DOW below 2.9℃ of an amount of nearly 5 000 m^3 is planned to be pumped up every day from a depth of about 350 m, and temporarily stored in a large simulated tank on land. DOW is then supplied to fish boats through hydrants distributed throughout the harbor and used for keeping salmon in clean and cold conditions. Ice made from DOW is also used for lowering temperature if necessary. DOW and ice made from DOW are also used during the transportation of fish and shellfish. The entire system is scheduled to be completed by the summer of 2005.
基金Supported by the National Marine Public Welfare Research Project of China(No.201305010)the National Natural Science Foundation of China(No.41106090)+5 种基金the Open Foundation of Key Laboratory of Marine Bio-resources Sustainable Utilization,Chinese Academy of Sciences(No.LMB111003)the Special Fund for Basic Scientific Research of Central Universities(No.21612401)the Natural Science Foundation of Guangdong Province(No.S2011040003113)the National Basic Research Program of China(973 Program)(No.2013CB956503)the Special Project for Science and Technology of Environmental Protection of Zhejiang Province(No.2011A31)the Wenzhou Science and Technology Plan Program(No.S2006A007)
文摘A 12-month program of monitoring potentially toxic microalgae(that produce lipophilic shellfi sh toxins; LSTs) and their toxins in bivalves was conducted from April 2006 to March 2007 in the Nanji Islands, East China Sea. Two Dinophysis species, D. caudata and D. acuminata, were identifi ed, and D. caudat a was found to be the dominant species. D. caudata was detected in water samples between April and June 2006, and between February and March 2007. It reached its highest abundances in May, with a mean abundance of 1.38×10 2 cells/L in surface water and 1.25×10 2 cells/L in bottom water(<10 m deep). The temporal distribution of D. caudata was associated with the occurrence of LSTs in bivalve samples, which mostly occurred at the same time as D. caudata blooms, between April and July 2006. All of the cultured bivalves sampled between April and June were contaminated with LSTs, with an average toxicity of 85 μg okadaic acid(OA) eq./100 g meat, which was four times higher than the Chinese regulatory limit(20 μg OA eq./100 g meat). Ten out of fi fteen wild samples(66.7%) collected during the same period were positive for LSTs, and contained an average LST toxicity of 45 μg OA eq./100 g meat(more than twice the regulatory value). Cultured Patinopecten yessoensis collected on 15 May 2006 had the highest toxicity, 320 μg OA eq./100 g meat, and relatively high toxicities(80 to 160 μg OA eq./100 g meat) were found in bivalves until the end of July.
文摘It has been classed 856 species of the mollusks which belong to 120 families and five classes living in western coast of the Tonkin Gulf. The class Gastropod was the most abundant, with 449 species, the Bivalve with 368 species, the Scaphopoda with 10 species, the Cephalopods with 19 species and the Amphineura with eight species. Standing stock was estimated about 366,749 tons, that annually caught 80,000 tons. The causes of the resource depletion were human and natural, but the human factors played a key role. The fundamental orientations for developing the resources include: exploiting during harvest seasons, planning suitable aquaculture, moderate exploitation so the resources can restore themselves, combining exploitation and conservation.
文摘Marine invertebrates and fish are well known for their remarkable genetic diversity, which is commonly explained by large population size and the characteristic dispersive nature of their early, planktonic life history. Other potential sources of diversity in marine animals, such as a higher mutation rate, have been much less considered, though evidence for a high genetic load in marine bivalves has been accumulating for nearly half a century. In this review, I examine evidence for a higher genetic load in marine animals from studies of molecular marker segregation and linkage over the last 40 years, and survey recent work examining mutational load with molecular evolution approaches. Overall, marine animals appear to have higher genetic load than terrestrial animals (higher dn/ds ratios, inbreeding load, and segregation dis'tortion), though results are mixed for marine fish and data are lacking for many marine animal groups. Bivalves (oysters) have the highest loads observed among marine animals, comparable only to long-lived plants; however, more data is needed from other bivalves and more marine invertebrate taxa generally. For oysters, a higher load may be related to a chronically lower effective population size that, in concert with a higher mutational rate, elevate the number of deleterious mutations observed. I suggest that future studies use high-throughput sequencing approaches to examine (1) polymorphism in genomescale datasets across a wider range of marine animals at the population level and (2) intergenerational mutational changes between parents and offspring in crosses of aquaculture species to quantify mutation rates.
基金supported by the National Social Science Fund(Grant Nos.11CKG004 and 11AZD116)Natural Science Foundation of Shandong Province(Grant No.ZR2012BQ025)Innovation Fund of Shandong University(Grant No.IFYT12038)
文摘The Beiqian site,located in Jimo city,Qingdao,went through three archaeological excavations,and a large number of human and animal bones in the Dawenkou culture and the Zhou Dynasty period were unearthed.By carbon and nitrogen stable isotope analysis,the diet of ancestors is expected to be recovered,and the domesticated plants and animals will be discussed.The investigated results showed that the food structure of ancestors in both periods included food crops,mainly C4 foods,and meat,mainly marine shellfish and livestock.For domestic pigs,they mainly eat C3 plants,and were affected by the lifestyle of ancestors.This study also showed that the diet of ancestors is the shellfish rather than marine fish despite the Beiqian site is located near the shoreline.We can conclude that the diet of ancestors in Dawenkou culture period included millet crops(C4plants),shellfish,marine food,and livestock,representing the lifestyle of farming,domestication,and fishing,supplemented by hunting.In the Zhou dynasty period the meat resource was enriched by domesticated animals and marine fisheries,and the living conditions were significantly improved.