Objective： To directly examine the effects ofcarnosine on neuronal excitation and inhibition in rat hippocampus in vivo. Methods： Artificial cerebrospinal fluid with carnosine was directly administrated over the exp...Objective： To directly examine the effects ofcarnosine on neuronal excitation and inhibition in rat hippocampus in vivo. Methods： Artificial cerebrospinal fluid with carnosine was directly administrated over the exposed rat hippocampus. The changes of neuron activity in the CA1 region of hippocampus were evaluated by orthodromically- and antidromically-evoked potentials, as well as paired-pulse stimulation paradigm. Results： In both orthodromic and antidromic response potentials, carnosine transformed population spikes （PSs） with single spike into epileptiform multiple spikes. In addition, similar to the effect of 7-aminobutyric acidA （GABAA） antagonist picrotoxin, camosine decreased paired-pulse stimulating depression significantly. However, no significant change was observed in the spontaneous field potentials during the application of carnosine. Conclusion： The results indicate a disinhibition-induced excitation effect of carnosine on the CA1 pyramidal neurons. It provides important information against the application of carnosine as a potential anticonvulsant in clinical treatment.展开更多
基金Project (Nos. 30570585 and 30770548) supported by the National Natural Science Foundation of China
文摘Objective： To directly examine the effects ofcarnosine on neuronal excitation and inhibition in rat hippocampus in vivo. Methods： Artificial cerebrospinal fluid with carnosine was directly administrated over the exposed rat hippocampus. The changes of neuron activity in the CA1 region of hippocampus were evaluated by orthodromically- and antidromically-evoked potentials, as well as paired-pulse stimulation paradigm. Results： In both orthodromic and antidromic response potentials, carnosine transformed population spikes （PSs） with single spike into epileptiform multiple spikes. In addition, similar to the effect of 7-aminobutyric acidA （GABAA） antagonist picrotoxin, camosine decreased paired-pulse stimulating depression significantly. However, no significant change was observed in the spontaneous field potentials during the application of carnosine. Conclusion： The results indicate a disinhibition-induced excitation effect of carnosine on the CA1 pyramidal neurons. It provides important information against the application of carnosine as a potential anticonvulsant in clinical treatment.
