[Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. （Leguminosae） and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean see...[Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. （Leguminosae） and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean seeds of A. edgeworthii, the morphological characteristics were observed and the appropriate temperature for germination was explored. For aerial seeds of A. edgeworthii, the characters of hard seeds were studied, and concentrated sulfuric acid treatment, hot water soak treat- ment and mechanical damage treatment were compared to explore the methods for breaking seed dormancy. [Result] The aerial seeds were oblate and averaged 3.38 mm in length, 3.02 mm in width, 1.88 mm in thickness, 15.32 g in thousand grain weight, with a hard seed rate after of up to 98% natural maturation. The subter- ranean seeds were approximately oblate with a maximum diameter of 15 mm and a hundred grain weight of （50.08-58.26 g）; among all the treatments for breaking hardseededness, cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods, whereas hot water soak treatment was the least effective method; constant temperature between 20 and 30 ℃ was optimum for the germination of aerial seeds, and alternative temperature of 30/20 ℃ was most appropriate for the germination of subterranean seeds. [Conclusion] Cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods to break the hardseededness of A. edgeworthii.展开更多
Mature seeds of H. salicifolia, collected from five provenances （i.e. Hanuman Chatti, Helang, Lata, Rambara and Janggal Chatti） in west Himalaya, India, were treated with stratification （at 4℃ for 15, 30 and 60 d...Mature seeds of H. salicifolia, collected from five provenances （i.e. Hanuman Chatti, Helang, Lata, Rambara and Janggal Chatti） in west Himalaya, India, were treated with stratification （at 4℃ for 15, 30 and 60 days） and in different concentrations of GA3 （5, 10, 20 mM）, KNO3 （50, 100, 200 mM） and Thiourea （50, 100, 200 mM） solution to determine the variations in seed germination. Results reveal that the germination rates of seeds from different provenances under different pre-sowing treatments are significantly increased compared to those in control （24%-30%）. The seeds treated with Thiourea （100 mM） have highest germination rate （76%-83% for different seed sources）, followed by those （63%-71% for different seed sources） pretreated with stratification （4℃, 30 days）. GA3 treatment significantly shortens the mean germination time （MGT） and improves seed germination percentage. Considering the practical applicability and cost effectiveness, thiourea （100 mM） and stratification （at 4℃） treatments for seed germination are recommended for mass multiplication through seeds of H. saliciifolia in village/forest nurseries of the west Himalaya, India.展开更多
基金Supported by the"Twelfth Five-Year"Key Project for Crop Breeding of Sichuan Province(YZGG4-6)the Special Support Program for the Scientific Research Personnel of Sichuan Agricultural University~~
文摘[Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. （Leguminosae） and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean seeds of A. edgeworthii, the morphological characteristics were observed and the appropriate temperature for germination was explored. For aerial seeds of A. edgeworthii, the characters of hard seeds were studied, and concentrated sulfuric acid treatment, hot water soak treat- ment and mechanical damage treatment were compared to explore the methods for breaking seed dormancy. [Result] The aerial seeds were oblate and averaged 3.38 mm in length, 3.02 mm in width, 1.88 mm in thickness, 15.32 g in thousand grain weight, with a hard seed rate after of up to 98% natural maturation. The subter- ranean seeds were approximately oblate with a maximum diameter of 15 mm and a hundred grain weight of （50.08-58.26 g）; among all the treatments for breaking hardseededness, cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods, whereas hot water soak treatment was the least effective method; constant temperature between 20 and 30 ℃ was optimum for the germination of aerial seeds, and alternative temperature of 30/20 ℃ was most appropriate for the germination of subterranean seeds. [Conclusion] Cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods to break the hardseededness of A. edgeworthii.
文摘Mature seeds of H. salicifolia, collected from five provenances （i.e. Hanuman Chatti, Helang, Lata, Rambara and Janggal Chatti） in west Himalaya, India, were treated with stratification （at 4℃ for 15, 30 and 60 days） and in different concentrations of GA3 （5, 10, 20 mM）, KNO3 （50, 100, 200 mM） and Thiourea （50, 100, 200 mM） solution to determine the variations in seed germination. Results reveal that the germination rates of seeds from different provenances under different pre-sowing treatments are significantly increased compared to those in control （24%-30%）. The seeds treated with Thiourea （100 mM） have highest germination rate （76%-83% for different seed sources）, followed by those （63%-71% for different seed sources） pretreated with stratification （4℃, 30 days）. GA3 treatment significantly shortens the mean germination time （MGT） and improves seed germination percentage. Considering the practical applicability and cost effectiveness, thiourea （100 mM） and stratification （at 4℃） treatments for seed germination are recommended for mass multiplication through seeds of H. saliciifolia in village/forest nurseries of the west Himalaya, India.
