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PTEN/PI3K信号转导相关蛋白在非小细胞肺癌组织中的表达及其意义 被引量:13
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作者 廖东卫 王莉 +1 位作者 张新光 刘铭球 《癌症》 SCIE CAS CSCD 北大核心 2006年第10期1238-1242,共5页
背景与目的:PTEN/PI3K信号转导途径能调节细胞的增殖与存活,与多种肿瘤的发生发展密切相关,但在肺癌中所起的作用及其相互关系尚不十分明确。本研究旨在探讨PTEN/PI3K信号转导相关蛋白IGF-1R、PTEN和PI3K在非小细胞肺癌(non-smallcelllu... 背景与目的:PTEN/PI3K信号转导途径能调节细胞的增殖与存活,与多种肿瘤的发生发展密切相关,但在肺癌中所起的作用及其相互关系尚不十分明确。本研究旨在探讨PTEN/PI3K信号转导相关蛋白IGF-1R、PTEN和PI3K在非小细胞肺癌(non-smallcelllungcarcinoma,NSCLC)中的表达及其意义。方法:利用免疫组织化学SP法检测59例NSCLC组织、19例转移的淋巴结组织、16例癌旁增生肺组织和7例正常肺组织中IGF-1R、PTEN和PI3K蛋白的表达。IGF-1R、PTEN和PI3K蛋白阳性率的差异比较采用卡方检验和Fisher确切概率法。结果:59例NSCLC组织中IGF-1R、PTEN和PI3K蛋白阳性率分别为:72.88%、27.12%和84.75%。IGF-1R以及PI3K蛋白的阳性率与NSCLC的临床病理特征无显著相关(P>0.05)。PTEN蛋白的阳性率与NSCLC的组织学类型、细胞分化程度无关(P>0.05),但与淋巴结转移显著相关(P=0.009)。NSCLC组及转移淋巴结组中PI3K、PTEN蛋白的阳性率与正常肺组织和癌旁增生肺组织相比,差异均有显著性(P<0.05)。59例NSCLC组织中IGF和PI3K之间具有显著正相关性(P=0.001),Pearson列联系数为0.432。PTEN和PI3K之间具有显著负相关(P=0.000),Pearson列联系数为0.505。结论:IGF-1R、PI3K蛋白的过表达和PTEN蛋白的低表达与肺癌的发生及浸润转移相关。 展开更多
关键词 肺肿瘤 PTEN P13K IGF-1R 免疫组化 浸润转
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Role of Tissue Factor Pathway Inhibitor-2 in Ovarian Tumor Migration and Invasion 被引量:2
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作者 仲任 黄瑞滨 宋善俊 《The Chinese-German Journal of Clinical Oncology》 CAS 2005年第1期53-55,68-69,共5页
Objective: To elucidate the relation between human tissue factor pathwayinhibitor-2 (TFPI-2) expression and ovarian tumor migration and invasion. Methods: Human TFPI-2expression vector pBos-Cite-neo/TFPI-2 was transfe... Objective: To elucidate the relation between human tissue factor pathwayinhibitor-2 (TFPI-2) expression and ovarian tumor migration and invasion. Methods: Human TFPI-2expression vector pBos-Cite-neo/TFPI-2 was transfected into ovarian tumor cells line A2780- Afterthe transfected cells were selected by G418, transfected and nontransfected cells were screened forTFPI-2 mRNA and protein by reverse transcription-polymerase chain reaction and Western blotanalysis, respectively. The number of transfected or nontransfected cells passing through membraneof Boyden chamber was counted as the basis assessing tumor cells migratory and invasive behaviors.Results: Expression of mRNA and protein of TFPI-2 was detectable in transfected cells. In invasionassay, the number of TFPI-2-expressing cells to traverse a Matrigel-coated membrane was obviouslydecreased compared with that of nonexpressing cells (59.3±6.5 vs 109.7±5.5, P 【 0.01); While inmigration assay, no significant difference through a noncoated membrane was observed amongtransfected and nontransfected cells (114.7±8.6 vs 127.3±7.1, P 】 0.05). Conclusion: Expression ofTFPI-2 may strongly inhibit the invasive ability of ovarian tumor cells in vitro, but has no effecton the migratory ability which provides an experimental basis for genotherapy of human ovariantumor. 展开更多
关键词 tissue factor pathway inhibitor-2 ovarian tumor INVASION MIGRATION
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