浸蛋米醋试制的研究

食醋和鸡蛋是两种营养食品,当鸡蛋浸入食醋后,生鸡蛋中各种有害微生物被杀灭,同时鸡蛋中钙、铁、钴、锌被溶出,蛋膜蛋清中溶菌酶被释放,蛋白质肽链部分断裂,蛋黄中释放卵磷脂、胆碱和生物素等。这些均有益于治病健身。我国汉朝名医张仲景就有鸡蛋浸醋的苦酒汤药方,晋朝名医葛洪有“鸡子七枚、苦汤渍之”的《肘后备急方》。近年来日本广为流行一种醋蛋,醋蛋风普及全国。

料液浸泡法快速制作水晶皮蛋

采用料液浸泡新配方快速制作水晶皮蛋,成本低,口感好,贮藏期可达10～12个月。1.料液配制(按0.5千克鸭蛋计算)生石灰120克,食用酒精30克,纯碱30克,大盐20克,大蒜泥3克,红茶末4克,醋酸锌10克,水550克。将所配料液入锅用文火慢煮至65℃～72℃撤火,将料液温度降至40℃即可。2.入缸浸蛋浸缸用瓦缸,将选好的蛋放进缸中,蛋面距缸口10厘米。

孵化场种蛋的消毒处理

1种蛋消毒方法 甲醛熏蒸消毒法对清洁度差的种蛋,每立方米用42 m L福尔马林（40%甲醛溶液）＋21 g高锰酸钾,温度24℃以上,湿度60%-80%条件下,密闭熏蒸20-25 min,可杀死蛋壳表面绝大多数病原体。

红心咸蛋快速腌制新法

红心咸蛋快速腌制法是对传统腌制方法加以改进创新而成。其特点是加工时间短,产品味道鲜美、色泽诱人,蛋清如凝脂白玉,蛋黄红且流油,吃到嘴里感到松、沙、油、嫩。其制作方法如下。

风味各异的咸蛋卤蛋五香蛋

鸡蛋是人们常用的美味食物之一,营养价值很高。鸡蛋吃法多种多样,烹饪方法也有很多种,下面几种制作方法,为家常常用,简单易行,喜爱蛋食食物的人们不妨一试。咸蛋料理2道咸蛋是平民食物,却能将平凡食物变成美味佳肴。每次想买咸鸭蛋,生怕食品安全问题而止步,这一年来在大卖场常看到有卖鸭蛋,正好拿来做咸鸭蛋。（一）自制咸蛋材料：新鲜鸭蛋（或鸡蛋）1打,

Effects of mutation on a new strain Leptospirillum ferriphilum YXW and bioleaching of gold ore

Leptospirillum ferriphilum YXW was isolated through serial dilution from mixed microorganisms enriched in AMD from Dexing copper mine in Jiangxi Province, China. It was mutated by ultrasonic, UV and microwave to collect more efficient strain for bioleaching of gold ore. Physiological and biochemical characteristics indicate that strain YXW is a strict chemoautotrophic microorganism, and the optimal condition for its growth is temperature of 40 °C and pH 1.5. After mutation by ultrasonic, UV and microwave, the density of bacterial cells reached 9×109, 8.4×109 and 4.3×108 mL-1, increased by 291%, 265%and 87%, respectively, compared with the original culture. The bacterial total protein activity was improved by microwave and UV mutations, but was reduced by ultrasonic. Mutations had effects on bioleaching of gold ore in sequence of microwave〉UV〉ultrasonic. During gold ore bioleaching, the bacterial mutant after mutation by microwave had the best effect on the extraction rates of arsenic and iron, which were 19.6%and 17.7%higher than that of the original strain after bioleaching for 10 d, respectively. The results suggested that the effects of mutation on bioleaching of gold ore may not be mainly due to increase of bacterial cells density, but may be mainly attributed to the improvement of bacterial total protein activity.

Effect Analysis on the Soaking Technics of Corn Wet Milling in the Laboratory

[Objective] The research aimed to find the optimal condition of corn starch production in the laboratory and analyze the soaking effect.[Method] The orthogonal test was used to determine the suitable technological condition.By the light microscope and the scanning electron microscope,the soaking effect was analyzed.[Result] The suitable soaking condition was：soaking time 48 h,soaking temperature 55 ℃ and SO2 concentration 0.2%.The microscopic analysis result was that the protein matrix was sufficiently decomposed in the suitable soaking condition.The soaking effect had the positive correlation with the decomposed degree of protein matrix.[Conclusion] The research provided the basis for the soaking technics research of corn starch in the laboratory.

Estimation of Digestible Energy Values of Plant Protein Supplement in Pig

[Objective] The aim of the study was to establish the effective and accurate formulas for estimating the digestible energy （DE） values of plant protein supplement in pig. [Method] By difference method with different amount of alternative feeds （20% -50%）, two4 x4 Latin- square-designed trials were taken on eight castrated male pigs [ Yorkshire x Landrace x Neijiang pig, initial body-weight： （46 ±2） kg ] to deter- mine the apparent digestible energy （ADE） of the eight kinds of plant protein supplement commonly used in China, that is, corn gluten meal （sol.）, soybean meal （ sol. ）, fababean, pea, rapeseed meal （ sol. ）, sesame meal （ sol. ）, rapeseed meal （ exp. ） and cotton seed meal （sol.）. [Resultl （1） Fiber was the most important factor to estimate the ADE of plant protein supplement in pigs, and ADF was the best one. （2） The most effective equations were as below： （ 1 ） OE （kJ/kg DM） = 14 741.86 - 185.01ADF＋54.01SCHO＋22.45CP （ R =0.988,RSD= 67.9,P〈0.01 ） ; （2） DE （kJ/kg DM） =22 223.26 -209.58ADF＋26.79SCHO-1.09GE （ Ff =0.989,RSD=66.9, P〈0.01 ） . [Conclusion] The accurate, practical and specific regression equations were established for DE prediction of plant protein supplement in pig.

日本“口笛”减肥法

"口笛"减肥法日本NHK国际放送广播电台在2008年年底,曾花了一周时间,用16种语言面向全世界广播介绍了日本"口笛"大流行的社会现象。日语中的"口笛"其实就是我们常说的吹口哨。日本"口笛"的演奏者认为"口笛"的基本功就是腹式呼吸,呼气的"吹音"和吸气的"吸音",伴着瑜伽。

A Study on the Meat and Bone Meal or Poultry By-product Meal as Protein Substitutes of Fishmeal in Concentrated Diets for Paralichthys olivaceus

A study was conducted to evaluate the effects of meat and bone meal （MBM） and poultry by-product meal （PBM） as the replacement of fishmeal in the diets on the growth performance, survival and apparent digestibility coefficient （ADC） of Japanese flounder （Paralichthys olivaceus）. The experimental diets included 0%, 20%, 40%, 60% and 80% MBM or PBM replacement of total fishmeal respectively. All diets were iso-nitrogenous and isocaloric. The results showed that there are no significant differences （P 〉0.05） in growth performance among the treatments fed with 0% -60% MBM replacement of fishmeal, while the percent weight gain （WG, % ）, body length gain （BLG, % ） and ADC significantly decrease when fishmeal is replaced by 80% MBM. The result showed also that there are no significant differences （P 〉0.05） in growth performance and ADC among all treatments fed with the diets with 0% -80% replacements of fishmeal with PBM.

Clinical signification of high-mobility group box 1protein(HMGB1) expression in infiltrating ductalcarcinoma breast tissue

Objective: Exploring the clinical signification of high-mobility group box 1 protein(HMGB1) expression in infiltrating ductal carcinoma(IDC) breast tissue. Methods: The expression of HMGB1 protein in IDC breast tissue was detected by immunohistochemistry, and the relations among size of tumour, lymph node metastasis, clinical staging, estrogen receptor(ER), progesterone receptor(PR) and human epidermal growth factor receptor 2(HER-2) were also analyzed. Results: Fortysix cases out of 60 cases of IDC breast tissue showed positive or strong positive HMGB1 expression(76.67%), statistical significance was observed between HMGB1 expression with clinical staging(P < 0.01), lymph node metastasis(P < 0.01), breast cancer ER(P < 0.05) and HER-2(P < 0.05), however same conclusion can not be drawn between HMGB1 with either size of tumour or PR expression(P > 0.05) in IDC breast tissue. Spearman analysis showed negative correlation between HMGB1 expression and ER, and positive correlation between HMGB1 expression and clinical staging, lymph node metastasis together with HER-2. Conclusion: It's promising that HMGB1 expression in IDC tissue can be one of biological indicators of poor prognosis.

Extractum trametes robiniophila murr protects murine podocyte via actin cytoskeleton stabilization

The Chinese medicine Extractum trametes robiniophila murr(ETRM)is the extract of a type of fungus.Recent studies have suggested that ETRM efficiently improves the effacement of podocyte foot processes in adriamycin(ADR)-induced nephrotic rats.In the present study,we aimed to assess whether ETRM modulated the actin rearrangements of podocytes and involved signaling molecules,includingα-actinin-4 and nephrin.Podocytes were treated with ADR(0.5μmol/L),ADR(0.5μmol/L)+dexamethasone(Dex)(1μmol/L),ADR(0.5μmol/L)+ETRM(10 mg/mL).The F-actin in the podocytes was stained by fluorescent phallotoxins and observed by confocal microscopy.The expression levels ofα-actinin-4 and nephrin were tested by real-time PCR and Western blotting analysis.The administration of ETRM could significantly prevent ADR-treated podocytes from actin rearrangement.Both ETRM and Dex could stabilize podocyte actin cytoskeletons.Moreover,α-actinin-4 might act as a potential target for ETRM functionality in podocyte actin rearrangements.However,pretreatment with ETRM could not inhibit the up-regulation of nephrin as a result of ADR treatment.ETRM could improve the cytoskeleton stability in ADR-induced actin rearrangement of podocytes via regulatingα-actinin-4 expression.

Outcome ofJudet＇s quadricepsplasty for knee contractures and the effect of local infiltration of epinephrine on reducing blood loss

Objective： To evaluate the effectiveness ofJudet＇s quadricepsplasty for treatment of knee contractures and to identify the effect of local infiltration of epinephrine on blood loss associated with this procedure. Methods： A retrospective cohort study was conducted in which all cases of knee contractures managed with Judet＇s quadricepsplasty from 1st January 2009 to 31st December 2013 were included and were divided into two groups. The epinephrine group included patients who were infiltrated with diluted epinephrine （1：400,000） along with xylocaine, around the operative field 15 min prior to the incision time, while the control group did not receive any infiltration. Judet＇s outcome, blood loss, drop in he- moglobin and required blood transfusion were noted for all patients and compared between both groups. Results： Most common preceding pathology identified for the development of knee contractures was periarticular fracture while ilizarov application was the most common etiology. Both groups were found similar in all preoperative characteristics except preoperative flexion contracture （p = 0.02）. All func- tional outcome measures including Judet＇s outcome were similar in both groups. In contrast, duration of surgery （p = 0.01）, blood loss （p = 0.02）, drop in hemoglobin （p = 0.01） and number of transfusions （p = 0.03） were significantly reduced in epinephrine group. Conclusion： Judet＇s quadricepsplasty is a useful procedure to increase the range of motion of rigid knees and local infiltration of epinephrine is effective in decreasing the amount of subsequent blood loss and transfusion requirements.

Cathepsin C promotes the progression of periapical periodontitis

Although the role of cathepsin C (Cat C) in inflammation is gradually being elucidated, its function in periapical periodontitis, which is one of the most common infectious diseases worldwide, has not been studied. This study evaluated a surgically-induced model of periapical periodontitis in cathepsin C (Cat C) knock-down (KD) mice, which was constructed with a tetracycline operator, to evaluate the role of Cat C in the pathogenesis and progression of periapical periodontitis. Our results showed, for the first time, that there was a statistically significant increase in the expression of Cat C as periapical periodontitis progressed;this increase started from 1 week after surgery and reached a peak at 3 weeks after surgery, before gradually decreasing. The volume of periapical bone resorption in Cat C KD mice was significantly smaller than that in wild-type mice at 3 and 4 weeks after surgery (P<0.05). Inflammatory cell infiltration into the apical tissues of wild-type mice was also significantly higher than that of Cat C KD mice. The expression of receptor activator of nuclear factor-j B ligand (RANKL) in wild-type mice was also higher than that in Cat C KD mice. The difference in the number of osteoclasts in the apical area between the two groups was statistically significant after 2 weeks. Correlation analysis showed that there was a significant correlation between Cat C and RANKL expression (r= 0.835). Therefore, our data indicated that Cat C promoted the apical inflammation and bone destruction in mice.