To achieve a visible inverse Bin-amphiphysin-Rvs （I-BAR）domain recombinant of missing in metastasis （MIM） protein,the green fluorescent protein （GFP）encoding gene was cloned at the terminal of MIM-I-BAR as a pro...To achieve a visible inverse Bin-amphiphysin-Rvs （I-BAR）domain recombinant of missing in metastasis （MIM） protein,the green fluorescent protein （GFP）encoding gene was cloned at the terminal of MIM-I-BAR as a probe.The DNA was successfully constructed on a 6xHis-tagged prokaryotic expression plasmid.The non-GFP labeled MIM-I-BAR encoding plasmid was also constructed as a control. Being successfully transformed into BL21 （DE3 ）cells,the GFP-conjugated MIM-I-BAR （MIM-I-BAR-GFP ） exhibits strong visible fluorescence,and the expression product can be easily detected by visual inspection, a fluorescence microscope, Western blot or ultraviolet and visible spectrophotometer. Moreover, examination of expression efficiency under various culture conditions revealed that the MIM-I-BAR-GFP gene has a high protein yield at 10 ℃,but not at the culture temperature of 37 ℃.This property is much different from that of the non-fluorescent MIM-I-BAR gene. This optimal expression condition is also proved to be feasible for protein production in midi-scale. The fluorescent recombinant MIM-I-BAR-GFP protein can serve as a useful tool in scientific research, biomedical application and pharmaceutical development.展开更多
AIM: To investigate the effects of ghrelin on delayed gastrointestinal transit in alloxan-induced diabetic mice. METHODS: A diabetic mouse model was established by intraperitoneal injection with alloxan. Mice were ran...AIM: To investigate the effects of ghrelin on delayed gastrointestinal transit in alloxan-induced diabetic mice. METHODS: A diabetic mouse model was established by intraperitoneal injection with alloxan. Mice were randomized into two main groups: normal mice group and diabetic mice group treated with ghrelin at doses of 0, 20, 50, 100 and 200 μg/kg ip. Gastric emptying (GE), intestinal transit (IT), and colonic transit (CT) were studied in mice after they had a phenol red meal following injection of ghrelin. Based on the most effective ghrelin dosage, atropine was given at 1 mg/kg 15 min before the ghrelin injection for each measurement. The mice in each group were sacrificed 20 min later and their stomachs, intestines, and colons were harvested immediately. The amount of phenol red was measured. Percentages of GE, IT, and CT were calculated. RESULTS: Percentages of GE, IT, and CT were significantly decreased in diabetic mice as compared to control mice (22.9 ± 1.4 vs 28.1 ± 1.3, 33.5 ± 1.2 vs 43.2 ± 1.9, 29.5 ± 1.9 vs 36.3 ± 1.6, P < 0.05). In the diabetic mice, ghrelin improved both GE and IT, but not CT. The most effective dose of ghrelin was 100 μg/kg and atropine blocked the prokinetic effects of ghrelin on GE and IT.CONCLUSION: Ghrelin accelerates delayed GE and IT but has no effect on CT in diabetic mice. Ghrelin may exert its prokinetic effects via the cholinergic pathway in the enteric nervous system, and therefore has therapeutic potential for diabetic patients with delayed upper gastrointestinal transit.展开更多
AIM: To evaluate the patterns with metastasis of gallbladder carcinoma in lymph nodes and liver. METHODS: A total of 45 patients who had radical surgery were selected. The patterns with metastasis of primary gallbladd...AIM: To evaluate the patterns with metastasis of gallbladder carcinoma in lymph nodes and liver. METHODS: A total of 45 patients who had radical surgery were selected. The patterns with metastasis of primary gallbladder carcinoma in lymph nodes and liver were examined histopathologically and classified as TNM staging of the American Joint Committee on Cancer. RESULTS: Of the 45 patients, 29 (64.4%) had a lymph node positive disease and 20 (44.4%) had a direct invasion of the liver. The frequency of involvement of lymph nodes was strongly influenced by the depth of the primary tumor (P= 0.0001). The postoperative survival rate of patients with negative lymph node metastasis was significantly higher than that of patients with positive lymph node metastasis (P= 0.004), but the postoperative survival rate of patients with Nl lymph node metastasis was not significantly different from that of patients with N2 lymph node metastasis (P= 0.3874). The postoperative survival rate of patients without hepatic invasion was significantly better than that of patients with hepatic invasion (P= 0.0177). CONCLUSION: Complete resection of the regional lymph nodes is important in advanced primary gallbladder carcinoma (PGC). The initial sites of liver spread are located mostly in segments IV and V. It is necessary to achieve negative surgical margins 2 cm from the tumor. In patients with hepatic hilum invasion, extended right hepatectomy with or without bile duct resection or portal vein resection is necessary for curative resection.展开更多
Frequency domain wave equation forward modeling is a problem of solving large scale linear sparse systems which is often subject to the limits of computational efficiency and memory storage. Conventional Gaussian elim...Frequency domain wave equation forward modeling is a problem of solving large scale linear sparse systems which is often subject to the limits of computational efficiency and memory storage. Conventional Gaussian elimination cannot resolve the parallel computation of huge data. Therefore, we use the Gaussian elimination with static pivoting （GESP） method for sparse matrix decomposition and multi-source finite-difference modeling. The GESP method does not only improve the computational efficiency but also benefit the distributed parallel computation of matrix decomposition within a single frequency point. We test the proposed method using the classic Marmousi model. Both the single-frequency wave field and time domain seismic section show that the proposed method improves the simulation accuracy and computational efficiency and saves and makes full use of memory. This method can lay the basis for waveform inversion.展开更多
Three goats were fed with ^15N-labelled rice straw to study the characteristics of digestion,assimilation,transformation and excretion of C and N compounds from rice straw.It was shown that the amount of ^15N transfor...Three goats were fed with ^15N-labelled rice straw to study the characteristics of digestion,assimilation,transformation and excretion of C and N compounds from rice straw.It was shown that the amount of ^15N transformed into the bodies of the two slaughtered goats accounted for 38.5 and 23.6% of the total amount of ^15N deposition of the experimental diet taken by each goat.The ^15N excreted through the feces and urine for the three goats accounted for 34.8,33.2 and 33.9% of the total amount of ^15N deposition in the feed of the 3 goats.The recovery of total ^15N for the two slaughtered goats were 73.3 and 57.5%,with the corresponding rates of ^15N loss 26.7 and 42.5% respectively.The digestibilities of total amino acids for Goats 1 and 3 were 68.7 and 54.0%,and the digestibilties of carbohydrates for the two goats were 74.8 and 67.7% respectively.展开更多
As a form of consumption based on the digital content and traits of goods and services,digital consumption is characterized by a strong dependence on the digital technology infrastructure,precise identification of con...As a form of consumption based on the digital content and traits of goods and services,digital consumption is characterized by a strong dependence on the digital technology infrastructure,precise identification of consumer demand,adaptability to new business modes,swift consumer feedback and response,and great market potentials.Under the dual driving forces of technology empowerment and consumption upgrade,China s digital consumption has been growing rapidly,digitalization is empowering traditional sectors of consumption,and the consumption of digital content is expanding.Consumer potential for digital consumption is being unleashed in low-tier cities,and new-generation consumers are gaining influence.With an increasing number of digital consumers,new consumption modes and services have proliferated thanks to the improving digital infrastructure.Growing digital consumption has created an increasing demand-pulling effect that spurs product and service innovation,corporate internal process digitalization,and changes in corporate organization and decision-making.Driven by digital consumption,industrial chain upgrade and restructuring and business mode innovations will improve user experience and firm efficiency,contributing to the quality,efficiency,and dynamism of the manufacturing industry.Amid surging digital consumption,the digital transition of manufacturing is still faced with some problems and challenges.It is important to deepen the user-centric concepts and modes of value creation,create a differentiated service system,optimize digital resource allocation,enhance digital brand management,and scale up R&D and innovation of products and services.展开更多
基金The National Basic Research Program of China(973Program)(No.2011CB933503)the National Natural Science Foundation of China for Key Project of International Cooperation(No.61420106012)China Postdoctoral Science Foundation(No.2013M541592)
文摘To achieve a visible inverse Bin-amphiphysin-Rvs （I-BAR）domain recombinant of missing in metastasis （MIM） protein,the green fluorescent protein （GFP）encoding gene was cloned at the terminal of MIM-I-BAR as a probe.The DNA was successfully constructed on a 6xHis-tagged prokaryotic expression plasmid.The non-GFP labeled MIM-I-BAR encoding plasmid was also constructed as a control. Being successfully transformed into BL21 （DE3 ）cells,the GFP-conjugated MIM-I-BAR （MIM-I-BAR-GFP ） exhibits strong visible fluorescence,and the expression product can be easily detected by visual inspection, a fluorescence microscope, Western blot or ultraviolet and visible spectrophotometer. Moreover, examination of expression efficiency under various culture conditions revealed that the MIM-I-BAR-GFP gene has a high protein yield at 10 ℃,but not at the culture temperature of 37 ℃.This property is much different from that of the non-fluorescent MIM-I-BAR gene. This optimal expression condition is also proved to be feasible for protein production in midi-scale. The fluorescent recombinant MIM-I-BAR-GFP protein can serve as a useful tool in scientific research, biomedical application and pharmaceutical development.
基金Supported by National Natural Science Foundation of China, No. 30400429
文摘AIM: To investigate the effects of ghrelin on delayed gastrointestinal transit in alloxan-induced diabetic mice. METHODS: A diabetic mouse model was established by intraperitoneal injection with alloxan. Mice were randomized into two main groups: normal mice group and diabetic mice group treated with ghrelin at doses of 0, 20, 50, 100 and 200 μg/kg ip. Gastric emptying (GE), intestinal transit (IT), and colonic transit (CT) were studied in mice after they had a phenol red meal following injection of ghrelin. Based on the most effective ghrelin dosage, atropine was given at 1 mg/kg 15 min before the ghrelin injection for each measurement. The mice in each group were sacrificed 20 min later and their stomachs, intestines, and colons were harvested immediately. The amount of phenol red was measured. Percentages of GE, IT, and CT were calculated. RESULTS: Percentages of GE, IT, and CT were significantly decreased in diabetic mice as compared to control mice (22.9 ± 1.4 vs 28.1 ± 1.3, 33.5 ± 1.2 vs 43.2 ± 1.9, 29.5 ± 1.9 vs 36.3 ± 1.6, P < 0.05). In the diabetic mice, ghrelin improved both GE and IT, but not CT. The most effective dose of ghrelin was 100 μg/kg and atropine blocked the prokinetic effects of ghrelin on GE and IT.CONCLUSION: Ghrelin accelerates delayed GE and IT but has no effect on CT in diabetic mice. Ghrelin may exert its prokinetic effects via the cholinergic pathway in the enteric nervous system, and therefore has therapeutic potential for diabetic patients with delayed upper gastrointestinal transit.
文摘AIM: To evaluate the patterns with metastasis of gallbladder carcinoma in lymph nodes and liver. METHODS: A total of 45 patients who had radical surgery were selected. The patterns with metastasis of primary gallbladder carcinoma in lymph nodes and liver were examined histopathologically and classified as TNM staging of the American Joint Committee on Cancer. RESULTS: Of the 45 patients, 29 (64.4%) had a lymph node positive disease and 20 (44.4%) had a direct invasion of the liver. The frequency of involvement of lymph nodes was strongly influenced by the depth of the primary tumor (P= 0.0001). The postoperative survival rate of patients with negative lymph node metastasis was significantly higher than that of patients with positive lymph node metastasis (P= 0.004), but the postoperative survival rate of patients with Nl lymph node metastasis was not significantly different from that of patients with N2 lymph node metastasis (P= 0.3874). The postoperative survival rate of patients without hepatic invasion was significantly better than that of patients with hepatic invasion (P= 0.0177). CONCLUSION: Complete resection of the regional lymph nodes is important in advanced primary gallbladder carcinoma (PGC). The initial sites of liver spread are located mostly in segments IV and V. It is necessary to achieve negative surgical margins 2 cm from the tumor. In patients with hepatic hilum invasion, extended right hepatectomy with or without bile duct resection or portal vein resection is necessary for curative resection.
基金supported by China State Key Science and Technology Project on Marine Carbonate Reservoir Characterization (No. 2008ZX05004-006)
文摘Frequency domain wave equation forward modeling is a problem of solving large scale linear sparse systems which is often subject to the limits of computational efficiency and memory storage. Conventional Gaussian elimination cannot resolve the parallel computation of huge data. Therefore, we use the Gaussian elimination with static pivoting （GESP） method for sparse matrix decomposition and multi-source finite-difference modeling. The GESP method does not only improve the computational efficiency but also benefit the distributed parallel computation of matrix decomposition within a single frequency point. We test the proposed method using the classic Marmousi model. Both the single-frequency wave field and time domain seismic section show that the proposed method improves the simulation accuracy and computational efficiency and saves and makes full use of memory. This method can lay the basis for waveform inversion.
文摘Three goats were fed with ^15N-labelled rice straw to study the characteristics of digestion,assimilation,transformation and excretion of C and N compounds from rice straw.It was shown that the amount of ^15N transformed into the bodies of the two slaughtered goats accounted for 38.5 and 23.6% of the total amount of ^15N deposition of the experimental diet taken by each goat.The ^15N excreted through the feces and urine for the three goats accounted for 34.8,33.2 and 33.9% of the total amount of ^15N deposition in the feed of the 3 goats.The recovery of total ^15N for the two slaughtered goats were 73.3 and 57.5%,with the corresponding rates of ^15N loss 26.7 and 42.5% respectively.The digestibilities of total amino acids for Goats 1 and 3 were 68.7 and 54.0%,and the digestibilties of carbohydrates for the two goats were 74.8 and 67.7% respectively.
文摘As a form of consumption based on the digital content and traits of goods and services,digital consumption is characterized by a strong dependence on the digital technology infrastructure,precise identification of consumer demand,adaptability to new business modes,swift consumer feedback and response,and great market potentials.Under the dual driving forces of technology empowerment and consumption upgrade,China s digital consumption has been growing rapidly,digitalization is empowering traditional sectors of consumption,and the consumption of digital content is expanding.Consumer potential for digital consumption is being unleashed in low-tier cities,and new-generation consumers are gaining influence.With an increasing number of digital consumers,new consumption modes and services have proliferated thanks to the improving digital infrastructure.Growing digital consumption has created an increasing demand-pulling effect that spurs product and service innovation,corporate internal process digitalization,and changes in corporate organization and decision-making.Driven by digital consumption,industrial chain upgrade and restructuring and business mode innovations will improve user experience and firm efficiency,contributing to the quality,efficiency,and dynamism of the manufacturing industry.Amid surging digital consumption,the digital transition of manufacturing is still faced with some problems and challenges.It is important to deepen the user-centric concepts and modes of value creation,create a differentiated service system,optimize digital resource allocation,enhance digital brand management,and scale up R&D and innovation of products and services.
