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全沟硬蜱Salp15蛋白家族成员基因的克隆与融合表达 被引量:1
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作者 王欣 孙毅 +1 位作者 牛思博 蒋宝贵 《寄生虫与医学昆虫学报》 CAS 2013年第2期102-109,共8页
为明确全沟硬蜱涎腺Salp15蛋白家族的基因表达情况,本文根据已知的Salp15序列设计引物对全沟硬蜱幼蜱的总RNA进行RT—PCR,克隆到可与莱姆病螺旋体外膜蛋白OspC互作的涎腺蛋白Salp15CDS序列,命名为I.Plarva5。对该蛋白做生物信息学... 为明确全沟硬蜱涎腺Salp15蛋白家族的基因表达情况,本文根据已知的Salp15序列设计引物对全沟硬蜱幼蜱的总RNA进行RT—PCR,克隆到可与莱姆病螺旋体外膜蛋白OspC互作的涎腺蛋白Salp15CDS序列,命名为I.Plarva5。对该蛋白做生物信息学预测,其肽链在N端含有信号肽,在c端含有与CD4分子的结合位点。与其他蜱种Salp15蛋白的相似性分别为:62.3%(北美的肩突硬蜱Ixodesscapularis)、74.5%(欧洲的篦子硬蜱Lricinusiric3)、59.4%(西伯利亚全沟硬蜱Lpersulcatus)、67.6%(日本地区的全沟硬蜱iperl)。将该cDNA序列分别加上EcoRI,SalI酶切位点,插入pMAL.c4X表达载体上,经IPTG诱导。在Transetta(DE3)上融合蛋白成功表达。此工作为研究全沟硬蜱的Salp15与莱姆病螺旋体,以及与宿主动物的相互关系奠定了基础。 展开更多
关键词 涎腺蛋白 全沟硬蜱 宿主免疫 克隆 表达
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The oncofetal protein IMP3 is an indicator of early recurrence and poor outcome in mucoepidermoid carcinoma of salivary glands 被引量:5
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作者 Mohamed R.Elshafey Rehab A.Ahmed +1 位作者 Mohamed I Mourad Essam T.Gaballah 《Cancer Biology & Medicine》 SCIE CAS CSCD 2016年第2期286-295,共10页
Objective: Mucoepidermoid carcinoma(MEC) is the most common primary malignancy of the salivary glands. Insulin-like growth factor-II m RNA-binding protein-3(IMP3) is an important prognostic factor in some cancers and ... Objective: Mucoepidermoid carcinoma(MEC) is the most common primary malignancy of the salivary glands. Insulin-like growth factor-II m RNA-binding protein-3(IMP3) is an important prognostic factor in some cancers and a tool that differentiates between benign and malignant pancreatic lesions. This study aimed to identify a relationship between the expression of IMP3 and the outcome of salivary gland MEC, as well as to differentiate MEC from pleomorphic adenoma(PA).Methods: Tissue specimens from 70 cases of salivary gland MEC, 40 cases of PA, and 10 cases with normal salivary gland were examined immunohistochemically for IMP3. The association among the expression of IMP3, clinicopathological characteristics and patient's survival was assessed.Results: IMP3 was present in 51.4% of MEC but absent in PA and normal salivary gland tissues. IMP3 expression was associated with age > 60 years, submandibular gland tumors, tumor size > 4 cm, high-grade tumors, lymph node metastasis, involvement of surgical margins, perineural invasion, distant metastasis, advanced TNM stage, tumor relapse, and death(P<0.05). Increased expression of IMP3, tumors of the submandibular gland, and lymph node metastasis were independent prognostic factors of disease-free survival(DFS). In addition, IMP3 was a strong predictor of overall survival(OS) together with distant metastasis and intermediate and high-grade tumors.Conclusions: IMP3 expression is highly important in evaluating the outcome of MEC. IMP3 can be used to differentiate MEC from PA of salivary glands. 展开更多
关键词 IMP3 mucoepidermoid carcinoma salivary glands PROGNOSIS
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